scholarly journals A Whey Fraction Rich in Immunoglobulin G Combined with Bifidobacterium longum subsp. infantis ATCC 15697 Exhibits Synergistic Effects against Campylobacter jejuni

2020 ◽  
Vol 21 (13) ◽  
pp. 4632
Author(s):  
Erinn M. Quinn ◽  
Michelle Kilcoyne ◽  
Dan Walsh ◽  
Lokesh Joshi ◽  
Rita M. Hickey
Keyword(s):  
Campylobacter Jejuni ◽  
Immunoglobulin G ◽  
Infant Nutrition ◽  
Synergistic Effects ◽  
Bifidobacterium Longum ◽  
Whey Proteins ◽  
Growth Curves ◽  
Sodium Metaperiodate ◽  
Biological Recognition ◽  
Ht 29

Evidence that whey proteins and peptides have health benefits beyond basic infant nutrition has increased dramatically in recent years. Previously, we demonstrated that a whey-derived immunoglobulin G-enriched powder (IGEP) enhanced adhesion of Bifidobacterium longum subsp. infantis ATCC 15697 (B. infantis) to HT-29 cells. In this study, we investigated the synergistic effect of IGEP-treated B. infantis on preventing the attachment of highly invasive Campylobacter jejuni 81–176 (C. jejuni) to intestinal HT-29 cells. The combination decreased the adherence of C. jejuni to the HT-29 cells by an average of 48% compared to the control (non-IGEP-treated B. infantis). We also confirmed that treatment of IGEP with sodium metaperiodate, which disables the biological recognition of the conjugated oligosaccharides, reduced adhesion of B. infantis to the intestinal cells. Thus, glycosylation of the IGEP components may be important in enhancing B. infantis adhesion. Interestingly, an increased adhesion phenotype was not observed when B. infantis was treated with bovine serum-derived IgG, suggesting that bioactivity was unique to milk-derived immunoglobulin-rich powders. Notably, IGEP did not induce growth of B. infantis within a 24 hours incubation period, as demonstrated by growth curves and metabolite analysis. The current study provides insight into the functionality of bovine whey components and highlights their potential in positively impacting the development of a healthy microbiota.

scholarly journals Bifidobacterium longum subsp. infantis ATCC 15697 and Goat Milk Oligosaccharides Show Synergism In Vitro as Anti-Infectives against Campylobacter jejuni

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 348 ◽  
Author(s):  
Erinn M. Quinn ◽  
Helen Slattery ◽  
Dan Walsh ◽  
Lokesh Joshi ◽  
Rita M. Hickey
Keyword(s):  
Campylobacter Jejuni ◽  
Bifidobacterium Longum ◽  
Goat Milk ◽  
Host Cells ◽  
Intestinal Cells ◽  
Milk Oligosaccharides ◽  
Bifidobacterium Strain ◽  
Ht 29 ◽  
Human Intestinal Cells

Bifidobacteria are known to inhibit, compete with and displace the adhesion of pathogens to human intestinal cells. Previously, we demonstrated that goat milk oligosaccharides (GMO) increased the attachment of Bifidobacterium longum subsp. infantis ATCC 15697 to intestinal cells in vitro. In this study, we aimed to exploit this effect as a mechanism for inhibiting pathogen association with intestinal cells. We examined the synergistic effect of GMO-treated B. infantis on preventing the attachment of a highly invasive strain of Campylobacter jejuni to intestinal HT-29 cells. The combination decreased the adherence of C. jejuni to the HT-29 cells by an average of 42% compared to the control (non-GMO treated B. infantis). Increasing the incubation time of the GMO with the Bifidobacterium strain resulted in the strain metabolizing the GMO, correlating with a subsequent 104% increase in growth over a 24 h period when compared to the control. Metabolite analysis in the 24 h period also revealed increased production of acetate, lactate, formate and ethanol by GMO-treated B. infantis. Statistically significant changes in the GMO profile were also demonstrated over the 24 h period, indicating that the strain was digesting certain structures within the pool such as lactose, lacto-N-neotetraose, lacto-N-neohexaose 3′-sialyllactose, 6′-sialyllactose, sialyllacto-N-neotetraose c and disialyllactose. It may be that early exposure to GMO modulates the adhesion of B. infantis while carbohydrate utilisation becomes more important after the bacteria have transiently colonised the host cells in adequate numbers. This study builds a strong case for the use of synbiotics that incorporate oligosaccharides sourced from goat′s milk and probiotic bifidobacteria in functional foods, particularly considering the growing popularity of formulas based on goat milk.

scholarly journals Identification, Characterization, and Antioxidant Potential of Bifidobacterium longum subsp. longum Strains Isolated From Feces of Healthy Infants

2021 ◽  
Vol 12 ◽  
Author(s):  
Li Zhao ◽  
Song Wang ◽  
Jiahuan Dong ◽  
Jialu Shi ◽  
Jiaqi Guan ◽  
...  
Keyword(s):  
Infant Nutrition ◽  
Bifidobacterium Longum ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Heat Shock Protein 60 ◽  
Intestinal Epithelial ◽  
Intact Cells ◽  
Bifidobacterium Animalis ◽  
Healthy Infants

Increasing evidence has indicated that oxidative stress is associated with the health of infants. Bifidobacterium, especially B. longum subsp. longum strains, are abundant in the gut microbiota of infants, which may have the potential to ameliorate oxidative damage. Thus, this study aimed to isolate and screen B. longum subsp. longum strains with probiotic characters and antioxidant properties as infants’ dietary supplements. In this study, 24 B. longum subsp. longum strains were isolated from 15 healthy infants identified via 16S rRNA and heat shock protein 60 (hsp60) sequences. B. longum subsp. longum B13, F2, K4, K5, K10, K13, and K15 strains were selected based on high values obtained from autoaggregation, hydrophobicity, and adhesion assays to HT-29 cells. Among these seven strains, B. longum subsp. longum F2, K5, K10, and K15 were selected according to the high tolerance of gastrointestinal tract conditions compared to Bifidobacterium animalis subsp. lactis BB-12. Among these four strains, B. longum subsp. longum K5 was susceptible to common antibiotics and showed the highest intestinal epithelial cell proliferation of CCD 841 CoN. Additionally, B. longum subsp. longum K5 showed a strong antioxidant capacity, and its supernatant exhibited better activity of reducing power, hydroxyl radical scavenging, and DPPH radical scavenging than that of the intact cells with cell-free extracts. The findings indicated that B. longum subsp. longum K5 could be used as a probiotic candidate in infant nutrition.

scholarly journals Synergistic Combinations of Curcumin, Sulforaphane, and Dihydrocaffeic Acid against Human Colon Cancer Cells

2020 ◽  
Vol 21 (9) ◽  
pp. 3108 ◽  
Author(s):  
Jesús Santana-Gálvez ◽  
Javier Villela-Castrejón ◽  
Sergio O. Serna-Saldívar ◽  
Luis Cisneros-Zevallos ◽  
Daniel A. Jacobo-Velázquez
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Synergistic Effects ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Acid Metabolite ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells ◽  
Synergistic Combinations ◽  
Ht 29

Nutraceutical combinations that act synergistically could be a powerful solution against colon cancer, which is the second deadliest malignancy worldwide. In this study, curcumin (C), sulforaphane (S), and dihydrocaffeic acid (D, a chlorogenic acid metabolite) were evaluated, individually and in different combinations, over the viability of HT-29 and Caco-2 colon cancer cells, and compared against healthy fetal human colon (FHC) cells. The cytotoxic concentrations to kill 50%, 75%, and 90% of the cells (CC50, CC75, and CC90) were obtained, using the MTS assay. Synergistic, additive, and antagonistic effects were determined by using the combination index (CI) method. The 1:1 combination of S and D exerted synergistic effects against HT-29 at 90% cytotoxicity level (doses 90:90 µM), whereas CD(1:4) was synergistic at all cytotoxicity levels (9:36–34:136 µM) and CD(9:2) at 90% (108:24 µM) against Caco-2 cells. SD(1:1) was significantly more cytotoxic for cancer cells than healthy cells, while CD(1:4) and CD(9:2) were similarly or more cytotoxic for healthy cells. Therefore, the SD(1:1) combination was chosen as the best. A model explaining SD(1:1) synergy is proposed. SD(1:1) can be used as a basis to develop advanced food products for the prevention/co-treatment of colon cancer.

Mechanism of synergistic effects of cytokine and hyperthermia on cytotoxicity in HT-29 and MCF-7 cells: Expression of MnSOD gene

1993 ◽  
Vol 18 (4) ◽  
pp. 269-274 ◽  
Author(s):  
Yong J. Lee ◽  
Zi-Zheng Hou ◽  
LindaLi Curetty ◽  
Joong M. Cho ◽  
Peter M. Corry
Keyword(s):  
Synergistic Effects ◽  
Ht 29 ◽  
Mnsod Gene ◽  
Mcf 7

Thermal denaturation of bovine immunoglobulin G and its association with other whey proteins

2017 ◽  
Vol 72 ◽  
pp. 350-357 ◽  
Author(s):  
Dimuthu Bogahawaththa ◽  
Jayani Chandrapala ◽  
Todor Vasiljevic
Keyword(s):  
Immunoglobulin G ◽  
Thermal Denaturation ◽  
Whey Proteins ◽  
Bovine Immunoglobulin

scholarly journals Detection and Characterization of Autoagglutination Activity by Campylobacter jejuni

2000 ◽  
Vol 68 (11) ◽  
pp. 6168-6175 ◽  
Author(s):  
Naoaki Misawa ◽  
Martin J. Blaser
Keyword(s):  
Campylobacter Jejuni ◽  
Natural Transformation ◽  
Host Cells ◽  
Vitro Assay ◽  
Sodium Metaperiodate ◽  
Bacterial Hydrophobicity ◽  
Phosphate Buffered Saline ◽  
Common Cell

ABSTRACT In several gram-negative bacterial pathogens, autoagglutination (AAG) activity is a marker for interaction with host cells and virulence. Campylobacter jejuni strains also show AAG, but this property varies considerably among strains. To examine the characteristics of C. jejuni AAG, we developed a quantitative in vitro assay. For strain 81-176, which shows high AAG, activity was optimal for cells grown for ≤24 h, was independent of growth temperature, and was best measured for cells suspended in phosphate-buffered saline at 25°C for 24 h. AAG activity was heat labile and was abolished by pronase or acid-glycine (pH 2.2) treatment but not by lipase, DNase, or sodium metaperiodate. Strain 4182 has low AAG activity, but extraction with water increased AAG, suggesting the loss of an inhibitor. Strain 6960 has weak AAG with no effect due to water extraction. Our study with clinical isolates suggests that C. jejuni strains may be grouped into three AAG phenotypes. A variant derived from strain 81116 that is flagellate but immotile showed the strong AAG exhibited by the parent strain, suggesting that motility per se is not necessary for the AAG activity. AAG correlated with both bacterial hydrophobicity and adherence to INT407 cells. Mutants which lack flagella (flaA,flaB, and flbA) or common cell surface antigen (peb1A) were constructed in strain 81-176 by natural transformation-mediated allelic exchange. Both AAG activity and bacterial hydrophobicity were abolished in the aflagellate mutants but not the peb1A mutant. In total, these findings indicate that C. jejuni AAG is highly associated with flagellar expression.

scholarly journals Inhibitory effect of goat and cow milk fermented by Bifidobacterium longum on Serratia marcescens and Campylobacter jejuni

2011 ◽  
Vol 24 (No. 4) ◽  
pp. 164-171 ◽  
Author(s):  
H. Pavlović ◽  
J. Hardi ◽  
V. Slačanac ◽  
M. Halt ◽  
D. Kocevski
Keyword(s):  
Serratia Marcescens ◽  
Campylobacter Jejuni ◽  
Bifidobacterium Longum ◽  
Goat Milk ◽  
Fermented Milk ◽  
Inhibitory Effect ◽  
Cow Milk ◽  
Fermentation Time ◽  
Ph Values ◽  
Fermentation Parameters

This study was performed to determine the influence of fermented goat and cow milk produced by the use of Bifidobacterium longum Bb-46 on pathogenic Serratia marcescens and Campylobacter jejuni strains. The correlation between the inhibitory effect and some fermentation parameters (the number of viable probiotic cells and pH of fermented milk) was also determined. Bifidobacterium longum counts and pH values were also measured in milk samples during fermentation. The results showed that the inhibitory effect of Bifidobacterium longum Bb-46 fermented goat milk on Serratia marcescens increased with the fermentation time. The highest inhibitory effect of fermented cow milk occurred in the middle course of fermentation. Statistically significant correlation between the inhibition degree of Serratia marcescens growth and pH values of fermented goat milk was noted as opposed to the correlation between the inhibition degree of Serratia marcescens growth and pH values of fermented cow milk which was not statistically significant. All samples of goat and cow fermented milk exhibited inhibitory effects on the growth of Campylobacter jejuni.  

Bovine immunoglobulin G, β-lactoglobulin, α-lactalbumin and serum albumin in colostrum and milk during the early post partum period

1999 ◽  
Vol 66 (3) ◽  
pp. 421-430 ◽  
Author(s):  
DIDIER LEVIEUX ◽  
ALAIN OLLIER
Keyword(s):  
Serum Albumin ◽  
Immunoglobulin G ◽  
Post Partum ◽  
Whey Proteins ◽  
Milk Supply ◽  
Holstein Friesian ◽  
The Mean ◽  
Bovine Immunoglobulin ◽  
Β Lactoglobulin ◽  
Friesian Cows

Colostrum and milk samples from 60 Holstein–Friesian cows were analysed for concentrations and yields of immunoglobulin G (IgG), β-lactoglobulin (β-lg), α-lactalbumin (α-la) and serum albumin (BSA) throughout the first 16 milkings post partum (8 d of lactation) using a single radial immunodiffusion assay. Concentrations (mg/ml, means±SD) at first milking were IgG 59·8±28·5, β-lg 14·3±4·6, α-la 2·04±0·6, BSA 1·21±0·44. Large variations were recorded for IgG concentrations (15·3–176·2 mg/ml) and yields (0·2–925 g). Cows in their first lactation produced significantly lower concentrations and yields of colostral IgG than cows in later lactations. A colostral yield of IgG below the 100 g required to prevent calf hypo-γ-globulinaemia was found in 18·3% of the cows. The concentrations of IgG, β-lg and BSA dropped abruptly in subsequent milkings and α-la concentration decreased slowly. The mean IgG concentration was <2 mg/ml after eight milkings and <1 mg/ml after fifteen milkings. However, IgG concentration did not differ significantly, at the 1% level, during milkings 11–15. The results were tabulated to make it possible to calculate the excess of whey proteins that would be obtained if early milks were illegally added to the milk supply.

scholarly journals Probiotic Colonization of the Adherent Mucus Layer of HT29MTXE12 Cells Attenuates Campylobacter jejuni Virulence Properties

2010 ◽  
Vol 78 (6) ◽  
pp. 2812-2822 ◽  
Author(s):  
Abofu Alemka ◽  
Marguerite Clyne ◽  
Fergus Shanahan ◽  
Thomas Tompkins ◽  
Nicolae Corcionivoschi ◽  
...  
Keyword(s):  
Cell Line ◽  
Campylobacter Jejuni ◽  
Bifidobacterium Longum ◽  
Early Time ◽  
Lactobacillus Helveticus ◽  
Mucus Layer ◽  
Junction Protein ◽  
Probiotic Treatment ◽  
Virulence Properties

ABSTRACT The HT29MTXE12 (E12) cell line harbors an adherent mucus layer, providing a novel technique to model mucosal infection in vitro. In this study, we have characterized the interaction of Campylobacter jejuni with the E12 cell line and exploited its unique mucus layer to examine the potential efficacy of probiotic treatment to attenuate C. jejuni virulence properties. C. jejuni 81-176 colonized and reproduced in E12 mucus. Adhesion to and internalization of C. jejuni were enhanced in E12 cells harboring mucus compared to parental cells without mucus. Translocation of C. jejuni occurred at early time points following infection. C. jejuni aligned with tight junctions and colocalized with the tight junction protein occludin, suggesting a paracellular route of translocation. Probiotic strains Lactobacillus rhamnosus R0011, Lactobacillus helveticus R0052, Lactobacillus salivarius AH102, Bifidobacterium longum AH1205, a commercial combination of L. rhamnosus R0011 and L. helveticus R0052 (Lacidofil), and a cocktail consisting of L. rhamnosus, L. helveticus, and L. salivarius (RhHeSa) colonized E12 mucus and bound to underlying cells. Probiotics attenuated C. jejuni association with and internalization into E12 cells and translocation to the basolateral medium of transwells. Live bacteria and prolonged precolonization of E12 cells with probiotics were necessary for probiotic action. These results demonstrate the potential for E12 cells as a model of mucosal pathogenesis and provide a rationale for the further investigation of probiotics as prophylaxis against human campylobacteriosis.

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