scholarly journals Impact of C57BL/6J and SV-129 Mouse Strain Differences on Ischemia-Induced Postnatal Angiogenesis and the Associated Leukocyte Infiltration in a Murine Hindlimb Model of Ischemia

2021 ◽  
Vol 22 (21) ◽  
pp. 11795
Author(s):  
Matthias Kübler ◽  
Philipp Götz ◽  
Anna Braumandl ◽  
Sebastian Beck ◽  
Hellen Ishikawa-Ankerhold ◽  
...  
Keyword(s):  
Mouse Strain ◽  
Tissue Damage ◽  
Macrophage Polarization ◽  
Inbred Mouse ◽  
Strain Differences ◽  
Mouse Strains ◽  
Leukocyte Infiltration ◽  
Hematoxylin And Eosin ◽  
Gastrocnemius Muscles ◽  
Shed Light

Strain-related differences in arteriogenesis in inbred mouse strains have already been studied excessively. However, these analyses missed evaluating the mouse strain-related differences in ischemia-induced angiogenic capacities. With the present study, we wanted to shed light on the different angiogenic potentials and the associated leukocyte infiltration of C57BL/6J and SV-129 mice to facilitate the comparison of angiogenesis-related analyses between these strains. For the induction of angiogenesis, we ligated the femoral artery in 8–12-week-old male C57BL/6J and SV-129 mice and performed (immuno-) histological analyses on the ischemic gastrocnemius muscles collected 24 h or 7 days after ligation. As evidenced by hematoxylin and eosin staining, C57BL/6J mice showed reduced tissue damage but displayed an increased capillary-to-muscle fiber ratio and an elevated number of proliferating capillaries (CD31+/BrdU+ cells) compared to SV-129 mice, thus showing improved angiogenesis. Regarding the associated leukocyte infiltration, we found increased numbers of neutrophils (MPO+ cells), NETs (MPO+/CitH3+/DAPI+), and macrophages (CD68+ cells) in SV-129 mice, whereas macrophage polarization (MRC1- vs. MRC1+) and total leukocyte infiltration (CD45+ cells) did not differ between the mouse strains. In summary, we show increased ischemia-induced angiogenic capacities in C57BL/6J mice compared to SV-129 mice, with the latter showing aggravated tissue damage, inflammation, and impaired angiogenesis.

scholarly journals Dysregulated Inflammatory Response to Candida albicans in a C5-Deficient Mouse Strain

2004 ◽  
Vol 72 (10) ◽  
pp. 5868-5876 ◽  
Author(s):  
Alaka Mullick ◽  
Miria Elias ◽  
Serge Picard ◽  
Lucie Bourget ◽  
Orce Jovcevski ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Inflammatory Response ◽  
Mouse Strain ◽  
Host Response ◽  
Inbred Mouse ◽  
Differential Susceptibility ◽  
Mouse Strains ◽  
Causative Role ◽  
Loss Of Function ◽  
Necrosis Factor Alpha

ABSTRACT Experimental infection of inbred mouse strains with Candida albicans provides a good model system to identify host genetic determinants that regulate onset of, response to, and ultimate outcome of disseminated candidiasis. The A/J mouse strain is exquisitely sensitive to infection with C. albicans, while the C57BL/6J strain is relatively resistant, as measured by survival following intravenous injection of Candida blastospores. This differential susceptibility is caused by an A/J-specific loss-of-function mutation in the C5 component of the complement pathway. C5 plays several critical roles in host response to infection, including target lysis and phagocyte recruitment. Therefore, to determine which of its functions were required for host resistance to candidiasis, a detailed comparative analysis of pathophysiology and host response to acute C. albicans infection was conducted in A/J and C57BL/6J mice. C5-sufficient C57BL/6J mice were found to succumb late in infection due to severe kidney pathology, typified by fungal replication and robust neutrophil-based inflammatory response associated with extensive tissue damage. In contrast, A/J mice were moribund within 24 h postinfection but displayed little if any kidney damage despite an inability to mobilize granulocytes and a high fungal load in the kidney. Rather, C5 deficiency in A/J mice was associated with higher levels of circulating cytokines tumor necrosis factor alpha, interleukin-6, monocyte chemotactic protein 1 (MCP-1), MCP-5, and eotaxin in response to C. albicans. Transfer of the C5-defective allele from A/J onto a C57BL/6J genetic background in recombinant congenic strain BcA17 recapitulated the phenotypic aspects of the susceptibility of A/J mice to C. albicans, confirming the causative role of C5 deficiency in the dysregulated cytokine response.

scholarly journals Working memory and pattern separation in founder strains of the BXD recombinant inbred mouse panel

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Price E. Dickson ◽  
Guy Mittleman
Keyword(s):  
Working Memory ◽  
Mouse Strain ◽  
Cognitive Abilities ◽  
Recombinant Inbred ◽  
Inbred Mouse ◽  
Strain Differences ◽  
Pattern Separation ◽  
Horizontal Distance ◽  
Recombinant Inbred Mouse ◽  
Genetic Mechanisms

AbstractWorking memory and pattern separation are fundamental cognitive abilities which, when impaired, significantly diminish quality of life. Discovering genetic mechanisms underlying innate and disease-induced variation in these cognitive abilities is a critical step towards treatments for common and devastating neurodegenerative conditions such as Alzheimer's disease. In this regard, the trial-unique nonmatching-to-location assay (TUNL) is a touchscreen operant conditioning procedure allowing simultaneous quantification of working memory and pattern separation in mice and rats. In the present study, we used the TUNL assay to quantify these cognitive abilities in C57BL/6J and DBA/2J mice. These strains are the founders of the BXD recombinant inbred mouse panel which enables discovery of genetic mechanisms underlying phenotypic variation. TUNL testing revealed that pattern separation was significantly influenced by mouse strain, whereas working memory was not. Moreover, horizontal distance and vertical distance between choice-phase stimuli had dissociable effects on TUNL performance. These findings provide novel data on mouse strain differences in pattern separation and support previous findings of equivalent working memory performance in C57BL/6J and DBA/2J mice. Although working memory of the BXD founder strains was equivalent in this study, working memory of BXD strains may be divergent because of transgressive segregation. Collectively, data presented here indicate that pattern separation is heritable in the mouse and that the BXD panel can be used to identify mechanisms underlying variation in pattern separation.

Endotoxin sensitivity of inbred mouse strains

1973 ◽  
Vol 19 (7) ◽  
pp. 767-769 ◽  
Author(s):  
Stephen I. Vas ◽  
Raymond S. Roy ◽  
Hugh G. Robson
Keyword(s):  
Mouse Strain ◽  
Inbred Mouse ◽  
Toxic Effects ◽  
Mouse Strains ◽  
Inbred Mouse Strains

Inbred mouse strains show characteristic susceptibility to S. typhimurium infections. The sensitivity of the same strains to endotoxin is not parallel. While C3H/He J and C57B1/6J mice were highly susceptible to infection they showed more resistance to purified endotoxin than A/J, a mouse strain relatively resistant to infection. These findings suggest that the death of mice during S. typhimurium infection is not due only to toxic effects of its lipopolysaccharide.

scholarly journals The Japanese Wild-Derived Inbred Mouse Strain, MSM/Ms in Cancer Research

Cancers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 1026
Author(s):  
Kazuhiro Okumura ◽  
Megumi Saito ◽  
Eriko Isogai ◽  
Yuichi Wakabayashi
Keyword(s):  
Mouse Strain ◽  
Inbred Mouse ◽  
Embryonic Stem ◽  
Bac Library ◽  
Inbred Mouse Strain ◽  
Wild Mouse ◽  
Artificial Chromosome ◽  
Mouse Strains ◽  
Inbred Mouse Strains ◽  
Genetic Traits

MSM/Ms is a unique inbred mouse strain derived from the Japanese wild mouse, Mus musculus molossinus, which has been approximately 1 million years genetically distant from standard inbred mouse strains mainly derived from M. m. domesticus. Due to its genetic divergence, MSM/Ms has been broadly used in linkage studies. A bacterial artificial chromosome (BAC) library was constructed for the MSM/Ms genome, and sequence analysis of the MSM/Ms genome showed approximately 1% of nucleotides differed from those in the commonly used inbred mouse strain, C57BL/6J. Therefore, MSM/Ms mice are thought to be useful for functional genome studies. MSM/Ms mice show unique characteristics of phenotypes, including its smaller body size, resistance to high-fat-diet-induced diabetes, high locomotive activity, and resistance to age-onset hearing loss, inflammation, and tumorigenesis, which are distinct from those of common inbred mouse strains. Furthermore, ES (Embryonic Stem) cell lines established from MSM/Ms allow the MSM/Ms genome to be genetically manipulated. Therefore, genomic and phenotypic analyses of MSM/Ms reveal novel insights into gene functions that were previously not obtained from research on common laboratory strains. Tumorigenesis-related MSM/Ms-specific genetic traits have been intensively investigated in Japan. Furthermore, radiation-induced thymic lymphomas and chemically-induced skin tumors have been extensively examined using MSM/Ms.

scholarly journals B6 Mouse Strain: The Best Fit for LPS-Induced Interstitial Cystitis Model

2021 ◽  
Vol 22 (21) ◽  
pp. 12053
Author(s):  
Ching-Hao Chen ◽  
Chun-Hou Liao ◽  
Kuo-Chiang Chen ◽  
Kuan-Lin Wang ◽  
Xiao-Wen Tseng ◽  
...  
Keyword(s):  
Interstitial Cystitis ◽  
Mouse Strain ◽  
Chronic Inflammatory Disease ◽  
Bladder Function ◽  
Mouse Strains ◽  
Control Group ◽  
Bladder Pain ◽  
Model Studies ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain

Interstitial cystitis (IC) is a chronic inflammatory disease characterized by bladder pain and increased urinary frequency. Although the C57BL/6J (B6) and FVB/NJ (FVB) mouse strains are commonly used as animal models for studies involving the urinary system, few reports have compared their lower urinary tract anatomy, despite the importance of such data. Our study aimed to characterize bladder function changes in FVB and B6 mouse strains with lipopolysaccharide (LPS)-induced IC, to understand mouse model-based bladder research. The bladder function parameters were measured by cystometrogram. Histological assay was examined by hematoxylin and eosin stain, Masson’s trichrome stain, and immunofluorescence staining. Results indicated that the two strains in the control group exhibited different bladder structures and functions, with significant anatomical differences, including a larger bladder size in the FVB than in the B6 strain. Furthermore, cystometry tests revealed differences in bladder function pressure. LPS-treated B6 mice presented significant changes in peak pressure, with decreased intercontraction intervals; these results were similar to symptoms of IC in humans. Each strain displayed distinct characteristics, emphasizing the care required in choosing the appropriate strain for bladder-model studies. The results suggested that the B6 mouse strain is more suitable for IC models.

scholarly journals Strain Differences in the Morphology of the Gametes of the Mouse

1959 ◽  
Vol 12 (1) ◽  
pp. 65 ◽  
Author(s):  
AWH Braden
Keyword(s):  
Inbred Mouse ◽  
Strain Differences ◽  
Sperm Head ◽  
Mouse Strains ◽  
Inbred Mouse Strains ◽  
Posterior Portion ◽  
Interstrain Differences

Characteristic differences in the morphology of the spenTI head have been noted between. the inbred mouse strains C57BL, CBA, A, and RIlL There were interstrain differences in the shape of the posterior portion of the sperm head and in the length and width of the .head. The spenTI of mice derived from certain interstrain crosses were also studied.

Genetic variability in forced and voluntary endurance exercise performance in seven inbred mouse strains

2002 ◽  
Vol 92 (6) ◽  
pp. 2245-2255 ◽  
Author(s):  
Imanuel Lerman ◽  
Brooke C. Harrison ◽  
Kalev Freeman ◽  
Timothy E. Hewett ◽  
David L. Allen ◽  
...  
Keyword(s):  
Exercise Performance ◽  
Treadmill Exercise ◽  
Inbred Mouse ◽  
Cardiac Contractility ◽  
Strain Differences ◽  
Voluntary Exercise ◽  
Mouse Strains ◽  
Apparent Relationship ◽  
Molecular Indicators ◽  
Voluntary Wheel

The goal of this study was to characterize the genetic contribution to both forced and voluntary exercise performance and to determine whether performance in these two paradigms is controlled by similar genetic influences. There were marked strain differences in treadmill exercise performance, with Swiss Webster (SW) and FVB/NJ mice showing elevated performance and C57BL/6J animals showing decreased performance compared with all other strains. There was no apparent relationship between treadmill performance and voluntary wheel performance, with the exception of SW mice, which demonstrated high performances on both the treadmill and the voluntary wheel. Numerous properties were measured to attempt to understand the basis for these differences in exercise performance. DBA/1J and SW mice exhibited significantly greater cardiac contractility than all other analyzed strains. Conversely, BALB/cByJ mice exhibited significantly reduced cardiac contractility compared with all other strains. Expression of molecular indicators of hypertrophy (atrial natriuretic factor and β-myosin heavy chain) was significantly elevated in DBA/2J myocardium compared with all other analyzed strains.

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