scholarly journals Bone Regeneration of a 3D-Printed Alloplastic and Particulate Xenogenic Graft with rhBMP-2

2021 ◽  
Vol 22 (22) ◽  
pp. 12518
Author(s):  
Ji-In Ryu ◽  
Byoung-Eun Yang ◽  
Sang-Min Yi ◽  
Hyo-Geun Choi ◽  
Sung-Woon On ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Bone Morphogenetic Protein 2 ◽  
Control Group ◽  
Group 12 ◽  
Defect Sites ◽  
Significant Difference ◽  
3D Printed

This study aimed to evaluate the bone regeneration capacity of a customized alloplastic material and xenograft with recombinant human bone morphogenetic protein-2 (rhBMP-2). We prepared hydroxyapatite (HA)/tricalcium phosphate (TCP) pure ceramic bone blocks made using a 3D printing system and added rhBMP-2 to both materials. In eight beagle dogs, a total of 32 defects were created on the lower jaws. The defective sites of the negative control group were left untreated (N group; 8 defects), and those in the positive control group were filled with particle-type Bio-Oss (P group; 12 defects). The defect sites in the experimental group were filled with 3D-printed synthetic bone blocks (3D group; 12 defects). Radiographic and histological evaluations were performed after healing periods of 6 and 12 weeks and showed no significant difference in new bone formation and total bone between the P and 3D groups. The 3D-printed custom HA/TCP graft with rhBMP-2 showed bone regeneration effects similar to that of particulate Bio-Oss with rhBMP-2. Through further study and development, the application of 3D-printed customized alloplastic grafts will be extended to various fields of bone regeneration.

scholarly journals Bone Regeneration Capability of 3D Printed Ceramic Scaffolds

2020 ◽  
Vol 21 (14) ◽  
pp. 4837 ◽  
Author(s):  
Ju-Won Kim ◽  
Byoung-Eun Yang ◽  
Seok-Jin Hong ◽  
Hyo-Geun Choi ◽  
Sun-Ju Byeon ◽  
...  
Keyword(s):  
3D Printing ◽  
Bone Regeneration ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Digital Light Processing ◽  
Defect Sites ◽  
Significant Difference ◽  
Printing System ◽  
3D Printed

In this study, we evaluated the bone regenerative capability of a customizable hydroxyapatite (HA) and tricalcium phosphate (TCP) scaffold using a digital light processing (DLP)-type 3D printing system. Twelve healthy adult male beagle dogs were the study subjects. A total of 48 defects were created, with two defects on each side of the mandible in all the dogs. The defect sites in the negative control group (sixteen defects) were left untreated (the NS group), whereas those in the positive control group (sixteen defects) were filled with a particle-type substitute (the PS group). The defect sites in the experimental groups (sixteen defects) were filled with a 3D printed substitute (the 3DS group). Six dogs each were exterminated after healing periods of 4 and 8 weeks. Radiological and histomorphometrical evaluations were then performed. None of the groups showed any specific problems. In radiological evaluation, there was a significant difference in the amount of new bone formation after 4 weeks (p < 0.05) between the PS and 3DS groups. For both of the evaluations, the difference in the total amount of bone after 8 weeks was statistically significant (p < 0.05). There was no statistically significant difference in new bone between the PS and 3DS groups in both evaluations after 8 weeks (p > 0.05). The proposed HA/TCP scaffold without polymers, obtained using the DLP-type 3D printing system, can be applied for bone regeneration. The 3D printing of a HA/TCP scaffold without polymers can be used for fabricating customized bone grafting substitutes.

scholarly journals Evaluation of the Sealing Ability of Three Obturation Techniques Using a Glucose Leakage Test

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Katarzyna Olczak ◽  
Halina Pawlicka
Keyword(s):  
Continuous Wave ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Thermal Methods ◽  
Root Canals ◽  
Anterior Teeth ◽  
Sealing Ability ◽  
Significant Difference

The aim of this study was to evaluate the sealing ability of three different canal filling techniques. Sixty-four roots of extracted human maxillary anterior teeth were prepared using ProTaper® rotary instruments. The specimens were then randomly divided into 3 experimental groups (n=16) and 2 control groups (n=8). The root canals were filled using cold lateral compaction (CLC group), continuous wave condensation technique using the Elements Obturation Unit® (EOU group), and ProTaper obturators (PT group). For the negative control group, 8 roots were filled using lateral compaction as in the CLC group, and the teeth were covered twice with a layer of nail varnish (NCG group). Another 8 roots were filled using lateral compaction, but without sealer, and these were used as the positive control (PCG group). A glucose leakage model was used for quantitative evaluation of microleakage for 24 hours and 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12 weeks. No significant difference in the cumulative amount of leakage was found between the three experimental groups at all observation times. The lateral condensation of cold gutta-percha can guarantee a similar seal of canal fillings as can be achieved by using thermal methods, in the round canals.

scholarly journals A Digital Microscopic Inspection of Dentinal Defects after Using Endodontic Retreatment Files

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Ammar AbuMostafa ◽  
Hala Almoqayyad ◽  
Al-Omari Mohammad
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Filling Material ◽  
Root Canals ◽  
Root Canal Retreatment ◽  
Significant Difference ◽  
Digital Microscope ◽  
Microscopic Inspection

Aim. The study aimed at evaluating the incidence of dentinal defects after root canal retreatment with ProTaper Universal retreatment (PTUR) and XP-endo Shaper and Finisher R (XP). Materials and Methods. Sixty extracted single-rooted human premolars were selected and divided into 4 groups of 15 teeth each. In the negative control group, the teeth were left unprepared. In the positive control group, the teeth were prepared with ProTaper Next and obturated with no further retreatment. In the PTUR and XP groups, the teeth were prepared and obturated followed by removal of the filling material at body temperature using PTUR and XP instruments, respectively. The roots were then sectioned at 3, 6, and 9 mm from the apex and observed under a digital microscope to detect defects. Results. PTUR group showed significantly higher ( p value <0.05) incidence of defects than the other groups. Comparison of no defects versus defects between groups in different areas of root canals demonstrated significant difference among the groups in the apical and cervical regions. Conclusion. Within the limitations of the present study, PTUR files created significantly more dentinal defects than XP files, with most of those defects at the cervical and apical areas of the root canals.

scholarly journals HISTOPATHOLOGICAL STRUCTURE OF WHITE RATS LIVER AFTER GIVING ANT NEST EXTRACT DUE TO INDUCED PARACETAMOL TOXIC DOSE

2019 ◽  
pp. 14
Author(s):  
I Made Merdana ◽  
I Made Kardena ◽  
Ketut Budiasa ◽  
I Made Dodi Gunawan
Keyword(s):  
Rat Liver ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Toxic Dose ◽  
White Rats ◽  
Significant Difference ◽  
Ant Nest ◽  
Histopathologic Changes

This study aim was to determine the influence ant nest plant extract (Myrmecodia pendans) on histopathological changeof white rat liver (Rattus novergicus) due to induced with paracetamol toxic dose. This study used 24 male white rats, divided into four groups, negative control group (P0) given placebo, positive control group (P1) given paracetamol dose 250 mg / kg bw for 10 days, P2 given ant nest extract 250 mg / kg bw and paracetamol dose 250 mg / kg bw for 10 days, P3 given ants nest extract 250 mg / kg bw for seven days, then continued by giving paracetamol and ants nest extract with dose 250 mg / kg bw for ten days. After the treatment done, all the rats were dinecropsed. Liver organs were taken and processed for making histopathology preparations. Parameters examined included hemorrhage, congestion, degeneration and necrosis. The data obtained were analyzed statistically by using Kruskal Wallis test followed by Mann Whitney test. Mann Whitney test results for all categories of histopathologic changes in  hemorrhagic, congestion, degeneration, and necrosis between negative control group (P0) and positive control group (P1) were significantly different (P <0.05), between negative control (P0) with P2 and P3 there was no significant difference (P> 0,05). Afterward, between the positive control (P1) and P2 with P3 there was a significant difference (P <0.05). I can be concludedthat the administration of paracetamol dose 250 mg/kg bw for 10 days affects the histopathologic changes of white rat liver. The administration of ant nest plant extracts can reduce the side effects of toxic doses of paracetamol.

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals The Effect of Tempeh Gembus Variations to Serum Levels of High Sensitivity C-Reactive Protein (hsCRP) and Serum Levels of Fibrinogen of Sprague Dawley Rats with Aterogenic Diet

2018 ◽  
Vol 25 (1) ◽  
pp. 91-97 ◽  
Author(s):  
Partika Kharunia Dewi ◽  
Diana Nur Afifah ◽  
Ninik Rustanti ◽  
Mohammad Sulchan ◽  
Gemala Anjani
Keyword(s):  
Serum Levels ◽  
Negative Control Group ◽  
Positive Control ◽  
Atherogenic Diet ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Standard Diet ◽  
Sprague Dawley ◽  
Significant Difference

Abstract Background and aims: Cardiovascular diseases are widespread and causes many deaths in the world. The concentration of acute phase protein: C-reactive protein (CRP) and fibrinogen will rise dramatically when inflammation happens, which that can be used as an early marker of cardiovascular disease risk. Tempeh gembus contains fiber, unsaturated fatty acids and isoflavones are believed to reduce the inflammatory reaction. The aim of the study was to determinate the effect of tempeh gembus variations to levels of hcCRP and levels of fibrinogen of Sprague Dawley rats with atherogenic diet. Material and methods: This study was quasi-experimental with posttest only randomized control group design using 35 Sprague Dawley mice. The rats were randomized into 5 groups: negative control group given the standard diet, the positive control group given standard diet and atherogenic diet, and three treatment groups were given the standard diet, atherogenic diet and variation of tempeh gembus (tempeh gembus, heated tempeh gembus and tempeh gembus with bromelain enzyme) for 28 days. Serum levels of hsCRP and fibrinogen examined using ELISA (Enzyme-linked Immunosorbent Assay). Results and conclusions: The administration of tempeh gembus with bromelain enzyme is the most effective treatment for hsCRP serum level indicated a significant difference (p=0.028) between the negative control group, positive control group and first group with the third group. Fibrinogen serum levels showed significant differences in all treatment groups (p =0.042), administration of tempeh gembus with bromelain enzyme is the most effective treatment is shown by a significant difference between the negative control group and the positive control group with third group. The administration of tempeh gembus with bromelain enzyme for 28 days can reduce the serum levels of hsCRP and fibrinogen on rats significantly.

scholarly journals Efektivitas ekstrak metanol batang pisang mauli 100%padapenyembuhan luka punggung mencit (Mus musculus)ditinjau dari jumlah sel radang (Effectiveness of 100%extract of Mauli Banana stem to healing process of mice’s (Mus musculus)back from the perspective of the number of inflamed cell)

2014 ◽  
Vol 13 (1) ◽  
pp. 33
Author(s):  
Maharani Laillyza Apriasari ◽  
Amy Nindia Carabelly ◽  
Gusti Febby Aprilia
Keyword(s):  
Mus Musculus ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Banana Plant ◽  
Significant Difference ◽  
Stem Extract ◽  
Banana Stem

Banana plant especially its trunk contains many kinds of fitokimia such as saponin, flavonoid, and tannin whichfunction as antibiotic and fasten the healing process. Mauli banana stem extract is often used by the people in HuluSungai Utara Banjarmasin Province to fasten healing skin injury. The purpose of this research is to histopathologicallyexamine the effectiveness of Mauli Banana stem extract to the healing process of the mice’s back (Mus musculus)onthe 3rd day. This purely true experimental research with post test only with control design used 27 mices which weredivided to 3 groups. Treatment group was given methanol extract of mauli banana stem whose average scores ofneutrofil and macrofag are representatively 2.333 and 1; negative control group was given aquadest with its averageneutrofil and macrofag scores are representatively 1.055 dan 1.678; and positive control group was given standardmedicine which contained Aloe veraextract with its average neutrofil and macrofag scores are representatively 1.066dan 1.667. The result of Kruskall-Wallis and Mann-Whiney showed a significant difference between treatment groupand negative control group. It can be concluded that the extract methanol of mauli banana stem on the healing processof injury to mancit’s back histopathologically influences the decreasing acute inflamed (neutrofil) cell and theincreasing chronic inflamed (macrophage) cell on the third day. In this case the extract of mauli banana stem ismedically potential to fasten the healing process of injury.

scholarly journals Topical application of snail mucin gel enhances the number of osteoblasts in periodontitis rat model

2019 ◽  
Vol 52 (2) ◽  
pp. 61
Author(s):  
H. Hendrawati ◽  
Hanindya Noor Agustha ◽  
Rezmelia Sari
Keyword(s):  
Treatment Group ◽  
Negative Control Group ◽  
Repair Process ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Important Indicator ◽  
Rat Models ◽  
Bone Damage ◽  
Significant Difference

Background: Repair of bone damage represents a fundamental issue in the treatment of periodontitis. The important indicator employed to monitor the bone damage repair process is the number of osteoblast cells. Achatina Fulica snail mucin (SM) contains glycosaminoglycans which have the potential to increase their number. However, the use of SM in dentistry remains limited. Purpose: To determine and prove the effect of SM gel in increasing the number of osteoblasts in rat models suffering from periodontitis. Methods: This study used 36 rat models divided into three groups, namely; a treatment group (T: 20% snail mucin gel, n = 12), a positive-control group (P: hyaluronic acid gel, n = 12) and a negative-control group (N: CMC-Na gel, n = 12). 0.2 ml of all material was applied to a pocket by means of a tuberculin syringe once a day for 14 days. Histologic observations using Haematoxylin-Eosin staining were carried out on days 3, 5, 7 and 14. Data was analyzed by two-way ANOVA followed by a post-hoc LSD. Results: A significant difference existed between the number of osteoblasts in the test groups. The highest number of osteoblasts observed was consistently that in the treatment group. Conclusion: The application of 20% snail mucin gel was effective in enhancing the number of osteoblasts in rats suffering from periodontitis.

scholarly journals Sealing Ability of Mineral Trioxide Aggregate, Biodentine and Glass Ionomer as Root-End Materials: A Question of Choice

2021 ◽  
Vol 49 (3) ◽  
pp. 232
Author(s):  
Aida Džanković ◽  
Naida Hadžiabdić ◽  
Samra Korać ◽  
Irmina Tahmiščija ◽  
Alma Konjhodžić ◽  
...  
Keyword(s):  
Mineral Trioxide Aggregate ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Dye Penetration ◽  
Anterior Teeth ◽  
Sealing Ability ◽  
Significant Difference ◽  
The Difference

<p class="Default"> </p><p class="Default"><strong><span>Objectives</span></strong><span>. The goal of this study was to determine the sealing ability of MTA, Biodentine, and Fuji IX as root-end materials after ultrasonic retrograde preparation using a dye penetration method. </span></p><p class="Default"><strong><span>Materials and Methods</span></strong><span>. Seventy permanent anterior teeth were used in this study. Root canals were prepared using rotary endodontic files, and obturated using the lateral condensa­tion technique. The resection of the root apices was performed perpendicularly to the long axis, and 3 mm deep retrocavities were made by ultrasonic tips. The samples were randomly divided into three experimental groups (N=20) and two control groups (N=5). The root-end cavities were filled with mineral-trioxide aggregate (Rootdent MTA, TehnoDent, Russia) in Group 1, Biodentine (Septodont, Saint Maur des Fossés, France) in Group 2 and Fuji IX GP (GC Corporation, Tokyo, Japan) in Group 3. The retrocavities of the positive control group were left unfilled, while retrocavities in the negative control group were sealed using a flowable composite material. The samples were made transparent, and dye penetration was assessed under a stereomi­croscope (x10). The data were analyzed with the Kruskal-Wallis test and the Mann-Whitney U-test. </span></p><p class="Default"><strong><span>Results</span></strong><span>. The Biodentine group had a lower mean leakage value than the MTA and Fuji IX groups, and the difference was statistically significant (P&lt;0.01). No statistical difference in apical microleakage was found between the MTA and Fuji IX groups (P&gt;0.05). </span></p><p class="Default"><strong><span>Conclusion</span></strong><span>. Within the limitations of this study, it was concluded that Biodentine provides better sealing ability as a root-end material than MTA and Fuji IX. There was no statistically significant difference between the sealing ability of MTA and Fuji IX as root-end materials.</span></p>

scholarly journals Spectrophotometric Analysis Evaluating Apical Microleakage in Retrograde Filling using GIC, MTA and Biodentine: An in-vitro study

2020 ◽  
Author(s):  
Manisha Nepal ◽  
Snigdha Shubham ◽  
Rupam Tripathi ◽  
Jwolan Khadka ◽  
Deepa Kunwar ◽  
...  
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Filling Materials ◽  
Diamond Bur ◽  
Retrograde Filling ◽  
Significant Difference ◽  
Supernatant Solution

Abstract Background The present study compares the apical microleakage of three different root-end filling materials in which the retrograde cavity is prepared by two different burs. Methods Eighty extracted single rooted maxillary and mandibular premolars were taken. Root canal treatment was completed. Apical 3 mm of all the teeth were resected with diamond disk. The tooth were divided into four groups with two subgroups for each group containing 10 tooth (N= 10) as: Group IA (Negative Control and IB (Positive Control); Group IIA and IIB: Prepared with round carbide bur and round diamond bur respectively, filled with GIC; Group IIIA and IIIB: Prepared with round carbide bur and round diamond bur respectively, filled with MTA; Group IVA and IVB: Prepared with round carbide bur and round diamond bur, filled with Biodentine. After applying two coats of nail varnish leaving apical 3 mm (except for negative control group) all teeth were immersed in 2% methylene blue for 3 days and again in 65% nitric acid for next 3 days for extraction of dye. The obtained solution was then transferred to eppendorf tube and centrifuged in microcentrifuges at 14,000 revolution per minutes (RPM) for 5 minutes. Optical density or absorbance of the supernatant solution was measured with UV spectrophotometer at 550 nm. Results The absorbance of the supernatant solution after dye extraction is decreasing in the order of positive control> GIC> MTA> Biodentine> negative control group. The significant difference was observed between GIC and MTA (p=0.0001) and GIC and Biodentine (p=0.0001) with two different burs but statistically non-significant difference was observed between MTA and Biodentine with Carbide bur (p=0.127) and Diamond bur (p=0.496) respectively. Conclusions Within the limitations of the present study, it can be concluded that Biodentine and MTA showed less microleakage as compared to GIC. There is no significant difference between mean microleakage of MTA and Biodentine. However, the mean OD of the Biodentine was least of all evaluated materials. Preparation of the root-end using round carbide bur as well as round diamond burs showed comparable microleakage for all three filling materials.

Sign in / Sign up

Export Citation Format

Share Document