scholarly journals Deficiency of T-Cell Intracellular Antigen 1 in Murine Embryonic Fibroblasts Is Associated with Changes in Mitochondrial Morphology and Respiration

2021 ◽  
Vol 22 (23) ◽  
pp. 12775
Author(s):  
Isabel Carrascoso ◽  
Beatriz Ramos Velasco ◽  
José M. Izquierdo
Keyword(s):  
T Cell ◽  
Rna Binding ◽  
Mitochondrial Dynamics ◽  
Rna Binding Protein ◽  
Mitochondrial Morphology ◽  
Embryonic Fibroblasts ◽  
Intracellular Antigen ◽  
Molecular Components ◽  
Shed Light

T-cell intracellular antigen 1 (TIA1) is a multifunctional RNA-binding protein involved in regulating gene expression and splicing during development and in response to environmental stress, to maintain cell homeostasis and promote survival. Herein, we used TIA1-deficient murine embryonic fibroblasts (MEFs) to study their role in mitochondria homeostasis. We found that the loss of TIA1 was associated with changes in mitochondrial morphology, promoting the appearance of elongated mitochondria with heterogeneous cristae density and size. The proteomic patterns of TIA1-deficient MEFs were consistent with expression changes in molecular components related to mitochondrial dynamics/organization and respiration. Bioenergetics analysis illustrated that TIA1 deficiency enhances mitochondrial respiration. Overall, our findings shed light on the role of TIA1 in mitochondrial dynamics and highlight a point of crosstalk between potential pro-survival and pro-senescence pathways.

scholarly journals Smooth muscle-specific HuR knockout induces defective autophagy and atherosclerosis

2021 ◽  
Vol 12 (4) ◽  
Author(s):  
Shanshan Liu ◽  
Xiuxin Jiang ◽  
Xiuru Cui ◽  
Jingjing Wang ◽  
Shangming Liu ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Cardiovascular Events ◽  
Rna Binding ◽  
Rna Binding Protein ◽  
Plaque Burden ◽  
Atherosclerotic Plaques ◽  
Ampk Activation ◽  
Homeostatic Regulation ◽  
Human Antigen R

AbstractHuman antigen R (HuR) is a widespread RNA-binding protein involved in homeostatic regulation and pathological processes in many diseases. Atherosclerosis is the leading cause of cardiovascular disease and acute cardiovascular events. However, the role of HuR in atherosclerosis remains unknown. In this study, mice with smooth muscle-specific HuR knockout (HuRSMKO) were generated to investigate the role of HuR in atherosclerosis. HuR expression was reduced in atherosclerotic plaques. As compared with controls, HuRSMKO mice showed increased plaque burden in the atherosclerotic model. Mechanically, HuR could bind to the mRNAs of adenosine 5′-monophosphate-activated protein kinase (AMPK) α1 and AMPKα2, thus increasing their stability and translation. HuR deficiency reduced p-AMPK and LC3II levels and increased p62 level, thereby resulting in defective autophagy. Finally, pharmacological AMPK activation induced autophagy and suppressed atherosclerosis in HuRSMKO mice. Our findings suggest that smooth muscle HuR has a protective effect against atherosclerosis by increasing AMPK-mediated autophagy.

scholarly journals Critical role of tristetraprolin and AU‐rich element RNA‐binding protein 1 in the suppression of cancer cell growth by globular adiponectin

FEBS Open Bio ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 1964-1976 ◽  
Author(s):  
Nirmala Tilija Pun ◽  
Amrita Khakurel ◽  
Aastha Shrestha ◽  
Sang‐Hyun Kim ◽  
Pil‐Hoon Park
Keyword(s):  
Cell Growth ◽  
Cancer Cell ◽  
Rna Binding ◽  
Binding Protein ◽  
Critical Role ◽  
Rna Binding Protein ◽  
Cancer Cell Growth ◽  
Globular Adiponectin

scholarly journals The control of inflammation via the phosphorylation and dephosphorylation of tristetraprolin: a tale of two phosphatases

2016 ◽  
Vol 44 (5) ◽  
pp. 1321-1337 ◽  
Author(s):  
Andrew R. Clark ◽  
Jonathan L.E. Dean
Keyword(s):  
Inflammatory Mediators ◽  
Knockout Mouse ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Human Orthologue ◽  
And Function ◽  
Detailed Picture ◽  
Lines Of Evidence

Twenty years ago, the first description of a tristetraprolin (TTP) knockout mouse highlighted the fundamental role of TTP in the restraint of inflammation. Since then, work from several groups has generated a detailed picture of the expression and function of TTP. It is a sequence-specific RNA-binding protein that orchestrates the deadenylation and degradation of several mRNAs encoding inflammatory mediators. It is very extensively post-translationally modified, with more than 30 phosphorylations that are supported by at least two independent lines of evidence. The phosphorylation of two particular residues, serines 52 and 178 of mouse TTP (serines 60 and 186 of the human orthologue), has profound effects on the expression, function and localisation of TTP. Here, we discuss the control of TTP biology via its phosphorylation and dephosphorylation, with a particular focus on recent advances and on questions that remain unanswered.

scholarly journals Role of RNA‐binding protein HuR and CUGBP1 in LPS‐induced Interleukin‐6 Expression in Macrophages

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Weibin Zha ◽  
Guangji Wang ◽  
Beth S. Pecora ◽  
Elaine Studer ◽  
Phillip B Hylemon ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein

scholarly journals The role of the RNA-binding protein Sam68 in mammary tumourigenesis

2010 ◽  
Vol 222 (3) ◽  
pp. 223-226 ◽  
Author(s):  
David J Elliott ◽  
Prabhakar Rajan
Keyword(s):  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein

Chitin binding protein as a possible RNA binding protein in Leishmania parasites

2020 ◽  
Vol 78 (1) ◽  
Author(s):  
Sajad Rashidi ◽  
Kurosh Kalantar ◽  
Celia Fernandez-Rubio ◽  
Enayat Anvari ◽  
Paul Nguewa ◽  
...  
Keyword(s):  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Novel Proteins ◽  
Transcriptional Gene Regulation ◽  
Chitin Binding Protein ◽  
Effective Drugs ◽  
Genus Leishmania ◽  
Leishmania Parasites

ABSTRACT Leishmaniasis includes a broad spectrum of pathological outcomes in humans caused by protozoan parasites from the genus Leishmania. In recent years, proteomic techniques have introduced novel proteins with critical functions in Leishmania parasites. Based on our report of a Chitin binding protein (CBP) in our previous immunoproteomic study, this article suggests that CBP might be an RNA binding protein (RBP) in Leishmania parasites. RBPs, as key regulatory factors, have a role in post-transcriptional gene regulation. The presence of RBPs in Leishmania parasites has not been considered so far; however, this study aims to open a new venue regarding RBPs in Leishmania parasites. Confirming CBP as an RBP in Leishmania parasites, exploring other RBPs and their functions might lead to interesting issues in leishmaniasis. In fact, due to the regulatory role of RBPs in different diseases including cancers and their further classification as therapeutic targets, the emerging evaluation of CBP and RBPs from Leishmania parasites may allow the discovery of novel and effective drugs against leishmaniasis.

scholarly journals The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging

2019 ◽  
Vol 73 (4) ◽  
pp. 775-787.e10 ◽  
Author(s):  
Davide D’Amico ◽  
Adrienne Mottis ◽  
Francesca Potenza ◽  
Vincenzo Sorrentino ◽  
Hao Li ◽  
...  
Keyword(s):  
Rna Binding ◽  
Mitochondrial Dynamics ◽  
Binding Protein ◽  
Rna Binding Protein
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