scholarly journals The TZM-bl Reporter Cell Line Expresses Kynureninase That Can Neutralize 2F5-like Antibodies in the HIV-1 Neutralization Assay

2022 ◽  
Vol 23 (2) ◽  
pp. 641
Author(s):  
Vladimir Morozov ◽  
Sylvie Lagaye ◽  
Alexey Morozov
Keyword(s):  
Cell Line ◽  
Neutralizing Antibodies ◽  
False Negative ◽  
Neutralization Assay ◽  
Reporter Cell Line ◽  
Reporter Cell ◽  
Negative Results ◽  
Viral Vaccine ◽  
Neutralizing Monoclonal Antibody ◽  
Hiv 1

Induction of broadly neutralizing antibodies targeting ectodomain of the transmembrane (TM) glycoprotein gp41 HIV-1 provides a basis for the development of a universal anti-viral vaccine. The HeLa cell-derived TZM-bl reporter cell line is widely used for the estimation of lentiviruses neutralization by immune sera. The cell line is highly permissive to infection by most strains of HIV, SIV, and SHIV. Here we demonstrated that TZM-bl cells express a 48 kDa non-glycosylated protein (p48) recognized by broadly neutralizing monoclonal antibody (mAb) 2F5 targeting the ELDKWA (aa 669–674) epitope of gp41TM of HIV-1. A significant amount of p48 was found in the cell supernatant. The protein was identified as human kynureninase (KYNU), which has the ELDKWA epitope. The protein is further called “p48 KYNU”. The HIV-1 neutralization by mAb 2F5 and 4E10 in the presence of p48KYNU was tested on Jurkat and TZM-bl cells. It was demonstrated that p48KYNU reduces neutralization by 2F5-like antibodies, but it has almost no effect on mAb 4E10. Therefore, p48KYNU can attenuate HIV-1 neutralization by 2F5-like antibodies and hence create false-negative results. Thus, previously tested immune sera that recognized the ELDKWA-epitope and demonstrated a “weak neutralization” of HIV-1 in TZM-bl assay should be reevaluated.

Use of a novel GFP reporter cell line to examine replication capacity of CXCR4- and CCR5-tropic HIV-1 by flow cytometry

2006 ◽  
Vol 131 (2) ◽  
pp. 134-142 ◽  
Author(s):  
Mark A. Brockman ◽  
Giancarlo O. Tanzi ◽  
Bruce D. Walker ◽  
Todd M. Allen
Keyword(s):  
Flow Cytometry ◽  
Cell Line ◽  
Replication Capacity ◽  
Reporter Cell Line ◽  
Reporter Cell ◽  
Gfp Reporter ◽  
Hiv 1

A Sensitive Reporter Cell Line for HIV-1tatActivity, HIV-1 Inhibitors, and T Cell Activation Effects

1994 ◽  
Vol 10 (3) ◽  
pp. 295-301 ◽  
Author(s):  
ESTUARDO AGUILAR-CORDOVA ◽  
JAVIER CHINEN ◽  
LARRY DONEHOWER ◽  
DOROTHY E. LEWIS ◽  
JOHN W. BELMONT
Keyword(s):  
T Cell ◽  
Cell Line ◽  
T Cell Activation ◽  
Cell Activation ◽  
Reporter Cell Line ◽  
Reporter Cell ◽  
Hiv 1

scholarly journals A High-Throughput Drug Screening System for HIV-1 Transcription Inhibitors

2006 ◽  
Vol 11 (7) ◽  
pp. 807-815 ◽  
Author(s):  
Mirjam-Colette Kempf ◽  
Jennifer Jones ◽  
Marintha L. Heil ◽  
Olaf Kutsch
Keyword(s):  
Cell Line ◽  
False Positive ◽  
De Novo ◽  
Drug Effects ◽  
Repeated Measurements ◽  
Cell Manipulation ◽  
Reporter Cell Line ◽  
Reporter Cell ◽  
Transcription Inhibitors ◽  
Hiv 1

Identification of HIV-1 transcription inhibitors was previously performed using infectivity assays. As de novo HIV-1 infection is highly sensitive to even minor compound toxicities, these assays are plagued by extremely high levels of false-positive hits. Hit identification is further complicated because infectivity assays lack target specificity. The authors demonstrate that it is possible to overcome these limitations by establishing a stable, chronically actively HIV-1-infected reporter cell line that exclusively identifies HIV-1 transcription inhibitors. In the reporter cell line, 2 spectrally separated fluorescence proteins serve as simultaneously accessible quantitative markers of HIV-1 expression and drug toxicity. The combined analysis of these markers drastically reduces the level of false-positive hits. As determination of fluorescence intensity in a plate-based format can be performed in a noninvasive manner, repeated measurements of fluorescence levels over several days after compound addition can be used to define the kinetic and dynamic characteristics of inhibitory drug effects. In addition, because of the stable nature of the reporter cell line, the assay requires no cell manipulation during assay preparation or assay analysis, rendering the system extremely cost-effective and reliable.

scholarly journals Generation of a NKX2.1 – p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4)

2020 ◽  
Vol 42 ◽  
pp. 101659
Author(s):  
Nora Drick ◽  
Anais Sahabian ◽  
Praeploy Pongpamorn ◽  
Sylvia Merkert ◽  
Gudrun Göhring ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Cell Line ◽  
Pluripotent Stem Cells ◽  
Reporter Cell Line ◽  
Reporter Cell ◽  
Induced Pluripotent

scholarly journals Discussing the Efficacy and Safety of Covid-19 Vaccine Available in India- A Mini Review

2021 ◽  
Vol 23 (11) ◽  
pp. 330-343
Author(s):  
Yogendra Shrestha ◽  
◽  
Jeet Bahadur Moktan ◽  
Renukaradhya Chitti ◽  
Shiv Kumar Yadav ◽  
...  
Keyword(s):  
Disease Transmission ◽  
Neutralizing Antibodies ◽  
False Negative ◽  
Emergency Situation ◽  
Epidemiological Studies ◽  
Self Awareness ◽  
Negative Results ◽  
Wide Range ◽  
The Government ◽  
Restricted Use

Background: Many variants detected after Wuhan-Hu-1 reference which were able to develop the resistance against the neutralizing antibodies induced by vaccine and may cause false negative results in diagnostic test. Novel variant B1.617 was detected in India and the Covid-19 cases hiked to its maximum; forcing the government towards approving a new vaccine for restricted use in emergency situation to cover a maximum population. Aims: This review looks at the efficacy, safety, and economical aspects of vaccines that have been authorized in India. Materials and methods: Wide-ranging assessment and analysis of accessible resources on online database. Results: The rAd26-s & rAd5-s demonstrate high efficacy as well as safety, followed by BBV152 and AZD1222. Various combinations of the vaccines with different platforms or vectors may induce wide range of immunity than a specific one. As per economical aspect, AZD1222 is more economical than the other two currently approved in India. Conclusion: There is a lack of clear end point to measure efficacy of the vaccine so the epidemiological studies with huge number of populations is required which may predict the perfect endpoint for efficacy measurement. Until then, inoculation with locally accessible vaccines and self-awareness about disease transmission prevention are the main options for reducing fatalities, protecting the health-care system, and eventually disease control.

scholarly journals Generation of a Jurkat-based fluorescent reporter cell line to evaluate lipid antigen interaction with the human iNKT cell receptor

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Piotr Humeniuk ◽  
Sabine Geiselhart ◽  
Claire Battin ◽  
Tonya Webb ◽  
Peter Steinberger ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Receptor ◽  
Inkt Cell ◽  
Reporter Cell Line ◽  
Reporter Cell ◽  
Fluorescent Reporter ◽  
Lipid Antigen ◽  
Antigen Interaction
Sign in / Sign up

Export Citation Format

Share Document