Soft Tissue Interface with Various Kinds of Implant Abutment Materials

Various materials, such as titanium, zirconia and platinum-gold (Pt-Au) alloy, have been utilized for dental implant trans-mucosal parts. However, biological understanding of soft tissue reaction toward these materials is limited. The aim of this study was to compare the response of cell lines and soft tissue to titanium, zirconia and Pt-Au substrata. The surface hydroxyl groups and protein adsorption capacities of the substrata were measured. Next, gingival epithelial-like cells (Sa3) and fibroblastic cells (NIH3T3) were cultured on the materials, and initial cell attachment was measured. Immuno-fluorescent staining of cell adhesion molecules and cytoskeletal proteins was also performed. In the rat model, experimental implants constructed from various materials were inserted into the maxillary tooth extraction socket and the soft tissue was examined histologically and immunohistochemically. No significant differences among the materials were observed regarding the amount of surface hydroxyl groups and protein adsorption capacity. Significantly fewer cells of Sa3 and NIH3T3 adhered to the Pt-Au alloy compared to the other materials. The expression of cell adhesion molecules and a well-developed cytoskeleton was observed, both Sa3 and NIH3T3 on each material. In an animal model, soft tissue with supracrestal tissue attachment was observed around each material. Laminin-5 immuno-reactivity was seen in epithelia on both titanium and zirconia, but only in the bottom of epithelia on Pt-Au alloy. In conclusion, both titanium and zirconia, but not Pt-Au alloy, displayed excellent cell adhesion properties.

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Expression of neural cell adhesion molecules and neurofilament protein isoforms in Ewing's sarcoma of bone and soft tissue sarcomas other than rhabdomyosarcoma

Human Pathology ◽

10.1016/s0046-8177(99)90039-4 ◽

1999 ◽

Vol 30 (10) ◽

pp. 1207-1212 ◽

Cited By ~ 16

Author(s):

W.M Molenaar ◽

F.L.H Muntinghe

Keyword(s):

Cell Adhesion ◽

Soft Tissue ◽

Adhesion Molecules ◽

Cell Adhesion Molecules ◽

Soft Tissue Sarcomas ◽

Neural Cell ◽

Protein Isoforms ◽

Neurofilament Protein ◽

Neural Cell Adhesion Molecules ◽

Neural Cell Adhesion

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Expression of cell-adhesion molecules in E. coli: a high-throughput method to identify paracellular modulators

10.1101/2021.04.08.439041 ◽

2021 ◽

Author(s):

Jay Rollins ◽

Tyler Worthington ◽

Emily Hooke ◽

Joseph Hobson ◽

Jacob Wengler ◽

...

Keyword(s):

Cell Adhesion ◽

Membrane Proteins ◽

Adhesion Molecules ◽

High Throughput ◽

Cell Adhesion Molecules ◽

Ultra Sound ◽

Adhesion Properties ◽

E Coli ◽

The Individual ◽

Extracellular Matrix Interactions

Cell-adhesion molecules (CAM) are membrane proteins responsible for cell-cell interactions or cell-extracellular matrix interactions. Among these proteins, claudins (CLDN), occludin (OCLN), and junctional adhesion molecules (JAM) are components of the tight junction (TJ), the single proteic structure tasked with safeguarding the paracellular space. The TJ is responsible for controlled permeability of blood-tissue barriers, regulating the passage of molecule passage by size and charge. Currently there is no translational solution to manipulate the TJ with the exception of Focused Ultra-sound (FUS) and Micro bubbling (MB) techniques, still in clinical trials. Here we describe the expression of TJ proteins in the outer membrane of E. coli and report its consequences. When expression is induced, the unicellular behavior of E. coli is replaced with multicellular aggregations that can be quantified using Flow Cytometry (FC). The adhesion properties of the aggregates are representative of the individual membrane proteins expressed. This method, called iCLASP (inspection of cell-adhesion molecules aggregation through FC protocols), allows the high-throughput interrogation of small-molecules influence on paracellular permeability, enabling for the first time the discovery of its modulators for therapeutic strategies.

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Dynamics of Cell Interactions and Communications during Melanoma Development

Critical Reviews in Oral Biology & Medicine ◽

10.1177/154411130201300107 ◽

2002 ◽

Vol 13 (1) ◽

pp. 62-70 ◽

Cited By ~ 54

Author(s):

G. Li ◽

K. Satyamoorthy ◽

M. Herlyn

Keyword(s):

Cell Adhesion ◽

Adhesion Molecules ◽

Intercellular Communication ◽

Cell Adhesion Molecules ◽

Cell Interactions ◽

Adhesion Properties ◽

Environmental Controls ◽

And Migration ◽

E Cadherin ◽

Cell Cell

Melanoma development not only involves genetic and epigenetic changes that take place within the cell, but also involves processes determined collectively by micro-environmental factors, including cell-cell interactions and communications. During the transition from normal cells to benign and malignant lesions, and subsequently to metastatic cancer, stepwise changes in intercellular communications provide tumor cells with the ability to overcome cell-cell adhesion and micro-environmental controls from the host and to invade surrounding tissues and disperse to distant locations. Cadherins are major cell–cell adhesion molecules involved in the development and maintenance of skin. E-cadherin expressed in normal melanocytes mediates growth and invasion control by keratinocytes. Progressive loss of E-cadherin and gain of N-cadherin during melanoma development not only free melanoma cells from control by keratinocytes, but also provide new adhesion properties, resulting in switched partnerships with fibroblasts and vascular endothelial cells. The cadherin subtype switching also dictates gap junctional specificity in melanocytic cells during tumor development. This selective intercellular communication may contribute to the regulation of cell growth, differentiation, apoptosis, and migration of melanocytic cells in both physiologic and pathologic conditions. Abnormal up-regulation of the immunoglobin repeat-containing cell adhesion molecules Mel-CAM and L1-CAM potentiates invasion and migration of melanoma. Thus, abnormal expression of intercellular adhesion receptors and dysregulated intercellular communication underlies melanoma development and progression.

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