Light-Photoreceptors and Proteins Related to Monilinia laxa Photoresponses

Light represents a ubiquitous source of information for organisms to evaluate their environment. The influence of light on colony growth and conidiation was determined for three Monilinia laxa isolates. The highest mycelial growth rate was observed under red light for the three M. laxa isolates, followed by green light, daylight or darkness. However, reduced sporulation levels were observed in darkness and red light, but conidiation enhancement was found under daylight, black and green light with more hours of exposure to light. Putative photoreceptors for blue (white-collar and cryptochromes), green (opsins), and red light (phytochromes) were identified, and the photoresponse-related regulatory family of velvet proteins. A unique ortholog for each photoreceptor was found, and their respective domain architecture was highly conserved. Transcriptional analyses of uncovered sets of genes were performed under daylight or specific color light, and both in time course illumination, finding light-dependent triggered gene expression of MlVEL2, MlPHY2, MlOPS2, and MlCRY2, and color light as a positive inductor of MlVEL3, MlVEL4, MlPHY1, and MlCRY1 expression. M. laxa has a highly conserved set of photoreceptors with other light-responsive fungi. Our phenotypic analyses and the existence of this light-sensing machinery suggest transcriptional regulatory systems dedicated to modulating the development and dispersion of this pathogen.

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Differential responses of coral larvae to the colour of ambient light guide them to suitable settlement microhabitat

Royal Society Open Science ◽

10.1098/rsos.150358 ◽

2015 ◽

Vol 2 (10) ◽

pp. 150358 ◽

Cited By ~ 21

Author(s):

Marie E. Strader ◽

Sarah W. Davies ◽

Mikhail V. Matz

Keyword(s):

Fluorescent Protein ◽

Critical Role ◽

Red Light ◽

Green Light ◽

Ambient Light ◽

Coral Larvae ◽

Acropora Millepora ◽

Light Sensing ◽

Natal Habitat

Reef-building corals produce planktonic planula larvae that must select an appropriate habitat to settle and spend the rest of their life, a behaviour that plays a critical role in survival. Here, we report that larvae obtained from a deep-water population of Pseudodiploria strigosa settled more readily under blue light and in the dark, which aligns well with the light field characteristics of their natal habitat. By contrast, larvae of the shallow-water coral Acropora millepora settled in high proportions under blue and green light while settlement was less in the dark. Acropora millepora larvae also showed reduced settlement under red light, which should be abundant at shallow depth. Hypothesizing that this might be a mechanism preventing the larvae from settling on the exposed upwards-facing surfaces, we quantified A. millepora settlement in manipulated light chambers in situ on the reef. While A. millepora larvae naturally preferred settling on vertical rather than exposed horizontal surfaces, swapping the colours of upwards-facing and sideways-facing light fields was sufficient to invert this preference. We also tested if the variation in intrinsic red fluorescence in A. millepora larvae correlates with settlement rates, as has been suggested previously. We observed this correlation only in the absence of light, indicating that larval red fluorescent protein is probably not directly involved in light sensing. Our study reveals previously under-appreciated light-sensory capabilities in coral larvae, which could be an important axis of ecological differentiation between coral species and/or populations.

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Green light perception paved the way for the diversification of GAF domain photoreceptors

10.1101/2021.09.27.462012 ◽

2021 ◽

Author(s):

Nibedita Priyadarshini ◽

Niklas Steube ◽

Dennis Wiens ◽

Rei Narikawa ◽

Annegret Wilde ◽

...

Keyword(s):

Red Light ◽

Visible Region ◽

Green Light ◽

Ancestral Sequence ◽

Light Harvesting Complexes ◽

Ph Titration ◽

Light Sensing ◽

Ancestral Sequence Reconstruction ◽

Sequence Reconstruction ◽

Light Signals

AbstractPhotoreceptors are proteins that sense incident light and then trigger downstream signaling events. Phytochromes are linear tetrapyrrole-binding photoreceptors present in plants, algae, fungi, and various bacteria. Most phytochromes respond to red and far-red light signals. Among the phytochrome superfamily, cyanobacteria-specific cyanobacteriochromes show much more diverse optical properties covering the entire visible region. Both phytochromes and cyanobacteriochromes share the GAF domain scaffold to cradle the chromophore as the light-sensing region. It is unknown what physiological demands drove the evolution of cyanobacteriochromes in cyanobacteria. Here we utilize ancestral sequence reconstruction and report that the resurrected ancestral cyanobacteriochrome proteins reversibly respond to green and red light signals. pH titration analyses indicate that the deprotonation of the bound phycocyanobilin chromophore enables the photoreceptor to perceive green light. The ancestral cyanobacteriochromes show modest thermal reversion to the green light-absorbing form, suggesting that they evolved to sense green-rich irradiance rather than red light, which is preferentially utilized for photosynthesis. In contrast to plants and green algae, many cyanobacteria can utilize green light for photosynthesis with their special light-harvesting complexes, phycobilisomes. The evolution of green/red sensing cyanobacteriochromes may therefore have allowed ancient cyanobacteria to acclimate to different light environments by rearranging the absorption capacity of the cyanobacterial antenna complex by chromatic acclimation.Significance StatementLight serves as a crucial environmental stimulus affecting the physiology of organisms across all kingdoms of life. Photoreceptors serve as important players of light responses, absorbing light and actuating biological processes. Among a plethora of photoreceptors, cyanobacteriochromes arguably have the wealthiest palette of color sensing, largely contributing to the success of cyanobacteria in various illuminated habitats. Our ancestral sequence reconstruction and the analysis of the resurrected ancestral proteins suggest that the very first cyanobacteriochrome most probably responded to the incident green-to-red light ratio, in contrast to modern red/far-red absorbing plant phytochromes. The deprotonation of the light-absorbing pigment for green light-sensing was a crucial molecular event for the invention of the new class of photoreceptors with their huge color tuning capacity.

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Postnatal growth rate and gonadal development in circadian tau mutant hamsters reared in constant dim red light

Reproduction ◽

10.1530/reprod/118.2.327 ◽

2000 ◽

pp. 327-330 ◽

Cited By ~ 7

Author(s):

RJ Lucas ◽

JA Stirland ◽

YN Mohammad ◽

AS Loudon

Keyword(s):

Time Course ◽

Red Light ◽

Somatic Growth ◽

Postnatal Growth ◽

Gonadal Development ◽

Testicular Development ◽

Wild Type ◽

Similar Time ◽

Syrian Hamsters ◽

Body Weights

The role of the circadian clock in the reproductive development of Syrian hamsters (Mesocricetus auratus was examined in wild type and circadian tau mutant hamsters reared from birth to 26 weeks of age under constant dim red light. Testis diameter and body weights were determined at weekly intervals in male hamsters from 4 weeks of age. In both genotypes, testicular development, subsequent regression and recrudescence exhibited a similar time course. The age at which animals displayed reproductive photosensitivity, as exhibited by testicular regression, was unrelated to circadian genotype (mean +/- SEM: 54 +/- 3 days for wild type and 59 +/- 5 days for tau mutants). In contrast, our studies revealed a significant impact of the mutation on somatic growth, such that tau mutants weighed 18% less than wild types at the end of the experiment. Our study reveals that the juvenile onset of reproductive photoperiodism in Syrian hamsters is not timed by the circadian system.

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Perancangan Sistem Kontrol Lampu Lalulintas Cerdas Dengan Menggunakan Mikrokontroler dan Kamera

Jurnal MIPA ◽

10.35799/jmuo.8.3.2019.26198 ◽

2019 ◽

Vol 8 (3) ◽

pp. 200

Author(s):

Tjerie Pangemanan ◽

Arnold Rondonuwu

Keyword(s):

Real Time ◽

Digital Image ◽

Red Light ◽

Green Light ◽

Processing Method ◽

Traffic Lights ◽

Traffic System ◽

Road Users ◽

Adjustment System ◽

System Time

Masalah lalu lintas merupakan salah satu masalah yang sangat sulit diatasi dengan hanya menggunakan system waktu (timer). Oleh sebab itu diperlukan suatu system pengaturan otomatis yang bersifat real-time sehingga waktu pengaturan lampu lalu lintas dapat disesuaikan dnegan keadaan di lapangan. Penelitian ini bertujuan mengembangkan suatu simulasi sistem yang mampu mengestimasi panjang antrian kendaraan menggunakan metoda pengolahan citra digital hanya dengan menggunakan satu kamera untuk dijadikan parameter masukan dalam menghitung lama waktu nyala lampu merah dan lampu hijau. Oleh karena itu, sistem lalulintas sangatlah diperlukan, sebagai sarana dan prasarana untuk menjadikan lalulintas lancar, aman, bahkan sebagai media pembelajaran disiplin bagi masyarakat pengguna jalan raya. Penelitian ini penulis menggunakan sistem pengontrolan berbasis citra digital dimana camera sebagai sensor. Untuk aplikasi dari semua metode dalam penelitian ini digunakan Microcontroller AurdinoTraffic problems is one of the problems that is very difficult to overcome by only using the system time (timer). Therefore we need an automatic real-time adjustment system so that the time settings for traffic lights can be adjusted according to the conditions on the ground. This study aims to develop a system simulation that is able to estimate the length of the vehicle queue using a digital image processing method using only one camera to be used as input parameters in calculating the length of time the red light and green light. Therefore, the traffic system is very necessary, as a means and infrastructure to make traffic smooth, safe, even as a medium for disciplined learning for road users. In this study the authors used a digital image-based control system where the camera as a sensor. For the application of all methods in this study, Aurdino Microcontroller is used

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Modeling Energy LED Light Consumption Based on an Artificial Intelligent Method Applied to Closed Plant Production System

Applied Sciences ◽

10.3390/app11062735 ◽

2021 ◽

Vol 11 (6) ◽

pp. 2735

Author(s):

Ernesto Olvera-Gonzalez ◽

Martín Montes Rivera ◽

Nivia Escalante-Garcia ◽

Eduardo Flores-Gallegos

Keyword(s):

Energy Consumption ◽

Energy Savings ◽

Nonlinear Models ◽

Light Emitting Diode ◽

Red Light ◽

Green Light ◽

Plant Production ◽

Production Plant ◽

Light Component ◽

Gp Model

Artificial lighting is a key factor in Closed Production Plant Systems (CPPS). A significant light-emitting diode (LED) technology attribute is the emission of different wavelengths, called light recipes. Light recipes are typically configured in continuous mode, but can also be configured in pulsed mode to save energy. We propose two nonlinear models, i.e., genetic programing (GP) and feedforward artificial neural networks (FNNs) to predict energy consumption in CPPS. The generated models use the following input variables: intensity, red light component, blue light component, green light component, and white light component; and the following operation modes: continuous and pulsed light including pulsed frequency, and duty cycle as well energy consumption as output. A Spearman's correlation was applied to generate a model with only representative inputs. Two datasets were applied. The first (Test 1), with 5700 samples with similar input ranges, was used to train and evaluate, while the second (Test 2), included 160 total datapoints in different input ranges. The metrics that allowed a quantitative evaluation of the model's performance were MAPE, MSE, MAE, and SEE. Our implemented models achieved an accuracy of 96.1% for the GP model and 98.99% for the FNNs model. The models used in this proposal can be applied or programmed as part of the monitoring system for CPPS which prioritize energy efficiency. The nonlinear models provide a further analysis for energy savings due to the light recipe and operation light mode, i.e., pulsed and continuous on artificial LED lighting systems.

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Artificial complementary chromatic acclimation gene expression system in Escherichia coli

Microbial Cell Factories ◽

10.1186/s12934-021-01621-3 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Dwi Ariyanti ◽

Kazunori Ikebukuro ◽

Koji Sode

Keyword(s):

Gene Expression ◽

Escherichia Coli ◽

Light Exposure ◽

Expression System ◽

Red Light ◽

Green Light ◽

Light Illumination ◽

Multiple Gene ◽

Gene Expression System ◽

Chromatic Acclimation

Abstract Background The development of multiple gene expression systems, especially those based on the physical signals, such as multiple color light irradiations, is challenging. Complementary chromatic acclimation (CCA), a photoreversible process that facilitates the control of cellular expression using light of different wavelengths in cyanobacteria, is one example. In this study, an artificial CCA systems, inspired by type III CCA light-regulated gene expression, was designed by employing a single photosensor system, the CcaS/CcaR green light gene expression system derived from Synechocystis sp. PCC6803, combined with G-box (the regulator recognized by activated CcaR), the cognate cpcG2 promoter, and the constitutively transcribed promoter, the PtrcΔLacO promoter. Results One G-box was inserted upstream of the cpcG2 promoter and a reporter gene, the rfp gene (green light-induced gene expression), and the other G-box was inserted between the PtrcΔLacO promoter and a reporter gene, the bfp gene (red light-induced gene expression). The Escherichia coli transformants with plasmid-encoded genes were evaluated at the transcriptional and translational levels under red or green light illumination. Under green light illumination, the transcription and translation of the rfp gene were observed, whereas the expression of the bfp gene was repressed. Under red light illumination, the transcription and translation of the bfp gene were observed, whereas the expression of the rfp gene was repressed. During the red and green light exposure cycles at every 6 h, BFP expression increased under red light exposure while RFP expression was repressed, and RFP expression increased under green light exposure while BFP expression was repressed. Conclusion An artificial CCA system was developed to realize a multiple gene expression system, which was regulated by two colors, red and green lights, using a single photosensor system, the CcaS/CcaR system derived from Synechocystis sp. PCC6803, in E. coli. The artificial CCA system functioned repeatedly during red and green light exposure cycles. These results demonstrate the potential application of this CCA gene expression system for the production of multiple metabolites in a variety of microorganisms, such as cyanobacteria.

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AB0612 THE FRACTURE PREVENTION UNIT OF THE VIRGEN MACARENA HOSPITAL REDUCES THE GAP IN THE MANAGEMENT OF OP, PARTICULARLY IN MALES AND MEETS IOF/ESCEO QUALITY STANDARDS

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2021-eular.1448 ◽

2021 ◽

Vol 80 (Suppl 1) ◽

pp. 1341.2-1341

Author(s):

B. Hernández-Cruz ◽

F. J. Olmo Montes ◽

M. J. Miranda García ◽

M. D. Jimenez Moreno ◽

M. A. Vázquez Gómez ◽

...

Keyword(s):

Fragility Fracture ◽

Red Light ◽

Green Light ◽

Quality Standards ◽

Type A ◽

Traffic Light ◽

Patient Identification ◽

Reference Time ◽

Fracture Liaison

Background:The Virgen Macarena University Hospital belongs to the Public Health System of Andalusia and serves 481,296 inhabitants in Seville, Spain. In 2018 the Fracture Liaison Service switched to a multidisciplinary unit.Objectives:To describe FLS, to know the characteristics of patients with emphasis on gender differences and to know the completion of International Osteoporosis Foundation quality standards.Methods:Prospective, observational, analytical, research of usual clinical practice. All the consecutive patients attended from May 2018 to October 2019, ≥50 years, with a fragility fracture (occurred in the previous 24 months) were included. The study was approved by the Ethics Committee, Code 1084-N-16.Results:Our FLS is a type A multidisciplinary Unit, with a high level of intervention in the evaluation, estimation of fracture risk and fall risk, treatment prescription and follow-up of the patients. We included 408 patients, 80% females, one third with ≥80 years. Fragility fractures recorded in 328 women were hip (132, 40%), clinical vertebral (81, 25%) and no hip no vertebral (115, 35%). Those recorded in 82 males were hip (53, 66%), clinical vertebral (20, 24%) and no hip no vertebral (9, 10%), p=0.0001. Males had a higher rate of secondary causes of OP, drinker, and smoking. The most relevant gender difference was the low percentage of patients receiving pre-FF OP treatment. Forty-nine (16%) women versus 9 (7%) males had received it at some point in their life, p=0.04. Two hundred and seventy-one (86%) women vs 48 males (63%) had received it at after their FF in their reference unit, and all them were treated after the FLS evaluation. The probability of a male not receiving prior treatment was 2.5 (95% CI 1.01- 6.51); p=0,04. This probability was 0.64 (0.38-1.09) after the FF. After twelve months of follow-up in FLs, 96% continued treatment, with no differences between men and women. The completion of IOF quality standards was bad (red light) for patient identification items and FLS reference time. It was poor (amber traffic light) for initial OP screening standard and was good (green light) for the remaining 10 indicators. The completion of IOF quality standards was bad (red light) for patient identification items and FLS reference time. It was poor (amber traffic light) for initial OP screening standard and was good (green light) for the remaining 10 indicators (Figure 1).Figure 1.Figure 1.Conclusion:The FLS is a multidisciplinary type A. Its operation has narrowed the gap in diagnosis, treatment, and follow-up of FF patients, especially males. It is essential to improve patient recruitment, reduce referral times and increase the overall assessment of the patients.References:[1]Ganda K. et al. Models of care for the secondary prevention of osteoporotic fractures: a systematic review and meta-analysis, Osteoporos Int 2013;24:293-406.[2]Javaid MK et al. A patient-level key performance indicator set to measure the effectiveness of fracture liaison services and guide quality improvement: a position paper of the IOF Capture the Fracture Working Group, National Osteoporosis Foundation and Fragility Fracture Network. Osteoporos Int. 2020 Jul;31(7):1193-1204.Acknowledgements:Spanish Society of Research in Mineral and Bone Metabolism for its support through the competitive project FLS Excellence 2018 to obtain a training grant from the case management nurse.Disclosure of Interests:Blanca Hernández-Cruz Speakers bureau: Sociedad Española de Reumatología, Abbvie, Roche, Bristol, MSD, Lilly, Pfizer, Amgen, Sanofi, Consultant of: Abbvie, Lilly, Sanofi, STADA, UCB, Amgen, Galapagos., Grant/research support from: Fundación para la Investigación Sevilla, Junta de AndalucíaFundación Andaluza de Reumatología, Sociuedad Española de Reumatología., Francisco Jesús Olmo Montes: None declared., Maria José Miranda García: None declared., María Dolores Jimenez Moreno: None declared., María Angeles Vázquez Gómez: None declared., Mercedes Giner García: None declared., Miguel Angel Colmenero Camacho: None declared., José Javier Pérez Venegas: None declared., María José Montoya García: None declared.

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Abundance Changes of the Response Regulator RcaC Require Specific Aspartate and Histidine Residues and Are Necessary for Normal Light Color Responsiveness

Journal of Bacteriology ◽

10.1128/jb.00762-08 ◽

2008 ◽

Vol 190 (21) ◽

pp. 7241-7250 ◽

Cited By ~ 10

Author(s):

Lina Li ◽

David M. Kehoe

Keyword(s):

Dna Binding ◽

Dynamic Range ◽

Response Regulator ◽

Red Light ◽

Green Light ◽

Binding Activity ◽

Transcriptional Responses ◽

Dna Binding Activity ◽

Normal Light ◽

Light Color

ABSTRACT RcaC is a large, complex response regulator that controls transcriptional responses to changes in ambient light color in the cyanobacterium Fremyella diplosiphon. The regulation of RcaC activity has been shown previously to require aspartate 51 and histidine 316, which appear to be phosphorylation sites that control the DNA binding activity of RcaC. All available data suggest that during growth in red light, RcaC is phosphorylated and has relatively high DNA binding activity, while during growth in green light RcaC is not phosphorylated and has less DNA binding activity. RcaC has also been found to be approximately sixfold more abundant in red light than in green light. Here we demonstrate that the light-controlled abundance changes of RcaC are necessary, but not sufficient, to direct normal light color responses. RcaC abundance changes are regulated at both the RNA and protein levels. The RcaC protein is significantly less stable in green light than in red light, suggesting that the abundance of this response regulator is controlled at least in part by light color-dependent proteolysis. We provide evidence that the regulation of RcaC abundance does not depend on any RcaC-controlled process but rather depends on the presence of the aspartate 51 and histidine 316 residues that have previously been shown to control the activity of this protein. We propose that the combination of RcaC abundance changes and modification of RcaC by phosphorylation may be necessary to provide the dynamic range required for transcriptional control of RcaC-regulated genes.

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