scholarly journals Efficacy of Disinfectants against Fusarium oxysporum f. sp. cubense Tropical Race 4 Isolated from La Guajira, Colombia

2021 ◽  
Vol 7 (4) ◽  
pp. 297
Author(s):  
Luisa F. Izquierdo-García ◽  
Sandra L. Carmona ◽  
Paola Zuluaga ◽  
Gustavo Rodríguez ◽  
Miguel Dita ◽  
...  
Keyword(s):  
Disease Management ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Contaminated Soil ◽  
Quaternary Ammonium ◽  
Contact Time ◽  
Soil Particles ◽  
Reproductive Structures ◽  
Race 4 ◽  
Ammonium Compounds

Banana, the main export fruit for Colombia, is threatened by Fusarium wilt (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), tropical race 4 (TR4). Pathogen containment through disinfecting tools, machinery, shoes, and any means that may carry contaminated soil particles with proper disinfectants is at the forefront of disease management. In this study, the biocide efficacy of 10 commercial quaternary ammonium compounds (QACs) products and one based on glutaraldehyde (GA) were evaluated on both reproductive structures (microconidia and macroconidia) and survival spores (chlamydospores) of Foc TR4 (strain 140038) isolated from La Guajira, Colombia. QACs were evaluated at 1200 ppm and two exposure times: <1 and 15 min in the absence or presence of soil. For GA disinfectant, four different concentrations (500, 800, 1200, and 2000 ppm) were evaluated at both contact times in the presence of soil. In the absence of soil, all QACs showed 100% biocidal efficiency against microconidia, macroconidia, and chlamydospores at both <1 and 15 min. The presence of soil decreased the efficacy of disinfectants, but some of them, such as QAC3_1st, QAC7_4th, and QAC5_4th, showed 98%, 98%, and 100% efficacy against Foc TR4 chlamydospores, respectively, after <1 min of contact time. For instance, the GA-based disinfectant was able to eliminate all Foc TR4 propagules after 15 min for all concentrations tested.

Effect of Temperature and Contact Time on the Activity of Eight Disinfectants - A Classification

1984 ◽  
Vol 47 (11) ◽  
pp. 841-847 ◽  
Author(s):  
P. GÉLINAS ◽  
J. GOULET ◽  
G. M. TASTAYRE ◽  
G. A. PICARD
Keyword(s):  
Quaternary Ammonium ◽  
Contact Time ◽  
Test Organism ◽  
Amphoteric Surfactant ◽  
Effect Of Temperature ◽  
Dilution Method ◽  
Ammonium Compound ◽  
Optimum Growth Temperature ◽  
Ammonium Compounds

The combined influence of temperature (4, 20, 37 and 50°C) and contact time (10, 20 and 30 min) on the efficacy of eight commercial disinfectants was evaluated by the Association of Official Analytical Chemists use-dilution method. An increase of temperature greatly enhanced the activity of all tested solutions, particularly glutaraldehyde, chlorhexidine acetate and the amphoteric surfactant, whereas contact time mainly enhanced the efficacy of sodium hypochlorite, the quaternary ammonium compound and the amphoteric surfactant. Temperature and contact time influenced the activity profile of the disinfectants tested, with a maximum efficacy near the optimum growth temperature (37°C) of the test organism (Pseudomonas aeruginosa ATCC 15442). This organism was highly resistant to the amphoteric surfactant as well as to the two quaternary ammonium compounds. Classification of disinfectants is proposed on the basis of their mode of action, temperature dependence and activation energies, heat and light stability, and tolerance to organic matter.

scholarly journals First Report of Fusarium Wilt on Cavendish Bananas, Caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (VCG 01213/16), in Vietnam

Plant Disease ◽  
2018 ◽  
Vol 102 (2) ◽  
pp. 448-448 ◽  
Author(s):  
T. N. Hung ◽  
N. Q. Hung ◽  
D. Mostert ◽  
A. Viljoen ◽  
C. P Chao ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
First Report ◽  
Race 4

scholarly journals The Distribution of Fusarium Wilt of Blackeyed Cowpeas within California Caused by Fusarium oxysporum f. sp. tracheiphilum Race 4

Plant Disease ◽  
1999 ◽  
Vol 83 (7) ◽  
pp. 694-694 ◽  
Author(s):  
S. N. Smith ◽  
D. M. Helms ◽  
S. R. Temple ◽  
C. Frate
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
San Joaquin Valley ◽  
University Of California ◽  
Crop Damage ◽  
Breeding Lines ◽  
1960S And 1970S ◽  
Single Field ◽  
The 1960S ◽  
Race 4

Fusarium wilt of blackeyed cowpeas has been known in California since the 1930s, and breeding for resistance to this disease pathogen has been a continuous effort. During the 1960s and 1970s, California Blackeye 5 (CB 5) cowpea (Vigna unguiculata L. Walp.), a widely grown cultivar of the time, became increasingly diseased by Fusarium oxysporum f. sp. tracheiphilum (Fot) Race 3 (2) throughout the growing regions of California. University of California cultivars CB 46 and CB 88 (1) were developed for resistance to Fot Races 1, 2, and 3. CB 46 is currently the principal blackeye cultivar grown on the majority of the acreage in the San Joaquin Valley. In 1989, a new race we designate “Fot Race 4” was isolated from wilted plants at a single field site in Stanislaus County. In years prior to identification, Fot Race 4 had caused severe wilt of CB 46 and CB 88 in this field. Even though the new Fot Race 4 remained confined to a small area for a number of years, sources of host plant resistance to Fot Race 4 were identified, hybridized, and screened, resulting in new progeny with desirable commercial agronomic characteristics. As observed in Stanislaus County, F. oxysporum f. sp. tracheiphilum Race 4 has the potential to cause serious crop damage, depending on virulence and soil inoculum levels, which may vary from year to year. In 1997 and 1998, an entirely different area in the southern San Joaquin Valley, about 140 miles from the original site in Stanislaus County, was found to have plants infected with Fot Race 4. Diseased plants were collected from patches in three separate CB 46 or CB 88 field sites in Tulare County. About 30 cultures were isolated from the diseased plants, which showed stunting, yellowing, and vascular discoloration. In greenhouse fusarium dip tests CB 46, CB 88, CB 5, and several Fot Race 4 resistant breeding lines were inoculated with all the collected isolates and evaluated. CB 46, CB 88, and CB 5 proved to be susceptible to these isolates, showing typical Fot Race 4 symptoms. The Fot Race 4 pathogen was then reisolated from greenhouse-grown, diseased stem tissue of CB 46, CB 88, and CB 5. These findings emphasize the importance of vigilance and necessity of continual disease surveys. They serve as an early alert for the University of California breeding program, and validate local cooperation with University of California Extension Farm Advisors. As a result of this effort new cultivar candidates with resistance to Fot Race 4 are in the final phases of multi-year commercial testing. References: (1) D. M. Helms et al. Crop Sci. 31:1703, 1991. (2) K. S. Rigert and K. W. Foster. Crop Sci. 27:220, 1987.

scholarly journals First Report of Fusarium oxysporum f. sp. cubense Tropical Race 4 Causing Fusarium Wilt of Cavendish Bananas in Israel

Plant Disease ◽  
2018 ◽  
Vol 102 (12) ◽  
pp. 2655 ◽  
Author(s):  
M. Maymon ◽  
U. Shpatz ◽  
Y. M. Harel ◽  
E. Levy ◽  
G. Elkind ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
First Report ◽  
Race 4

scholarly journals Evaluation of the Efficacy of Commercial Disinfectants Against Fusarium oxysporum f. sp. cubense Race 1 and Tropical Race 4 Propagules

Plant Disease ◽  
2019 ◽  
Vol 103 (4) ◽  
pp. 721-728 ◽  
Author(s):  
T. V. Nguyen ◽  
L. T. T. Tran-Nguyen ◽  
C. L. Wright ◽  
P. Trevorrow ◽  
K. Grice
Keyword(s):  
Fusarium Oxysporum ◽  
Sodium Hypochlorite ◽  
Active Ingredient ◽  
Panama Disease ◽  
Banana Production ◽  
Race 1 ◽  
Race 4 ◽  
On Farm ◽  
Farm Biosecurity ◽  
Ammonium Compounds

Panama disease caused by Fusarium oxysporum f. sp. cubense has devastated banana production worldwide. This work aimed to determine effective disinfectants against two races of F. oxysporum f. sp. cubense, race 1 and tropical race 4 (TR4), for implementation with on-farm biosecurity procedures against this disease following the outbreak of TR4 in North Queensland in 2015. A total of 32 commercial disinfectants were screened and their activity was assessed after ≤30 s, 5 min, 30 min, and 24 h of contact with an F. oxysporum f. sp. cubense suspension containing 105 chlamydospores/ml without and with soil added (0.05 g/ml). Of the disinfectants tested, the quaternary ammonium compounds containing ≥10% active ingredient were found to be the most effective against both F. oxysporum f. sp. cubense races. These products, when used at a 1:100 dilution, completely inhibited the survival of all F. oxysporum f. sp. cubense propagules across all the contact times regardless of the absence or presence of soil. The bioflavonoid product EvoTech 213 and bleach (10% sodium hypochlorite) used at a 1:10 dilution also eliminated all F. oxysporum f. sp. cubense propagules across all the contact times. None of the detergent-based or miscellaneous products tested were completely effective against both F. oxysporum f. sp. cubense races even used at a 1:10 dilution. Soil decreases the efficacy of disinfectants and therefore must be removed from contaminated items before treatments are applied.

scholarly journals Non-Synergistic Effect of Trichoderma harzianum and Glomus spp. in Reducing Infection of Fusarium Wilt in Banana

Pathogens ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 43 ◽  
Author(s):  
Arfe Castillo ◽  
Cecirly Puig ◽  
Christian Cumagun
Keyword(s):  
Biological Control ◽  
Synergistic Effect ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Trichoderma Harzianum ◽  
Root Weight ◽  
Biological Control Agents ◽  
Competitive Effects ◽  
Glomus Spp ◽  
Race 4

Philippine banana is currently threatened by Fusarium oxysporum f. sp. cubense Tropical Race 4 (FocR4). This study investigated the use of Trichoderma harzianum pre-treated with Glomus spp, as a means of managing Fusarium wilt on young ‘Lakatan’ banana seedlings. Results showed that Glomus applied basally significantly improved banana seedling growth with increased increment in plant height and pseudostem diameter and heavier root weight. The application of Glomus spp. alone offered 100% protection to the ‘Lakatan’ seedlings against FocR4 as indicated by the absence of the wilting symptom. A combination of T. harzianum and Glomus spp. also gave significant effect against Fusarium wilt through delayed disease progression in the seedlings but was not synergistic. Competitive effects were suspected when application of the two biological control agents on banana roots was done simultaneously.

Characterization of current Fusarium oxysporum f. sp. vasinfectum isolates from cotton in the San Joaquin Valley of California and Lower Valley El Paso Texas

Plant Disease ◽  
2020 ◽  
Author(s):  
Josue Diaz ◽  
Jorge Garcia ◽  
Celeste Lara ◽  
Robert B. Hutmacher ◽  
Mauricio Ulloa ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
El Paso ◽  
Resistance Mechanisms ◽  
Development Stage ◽  
Seedling Development ◽  
San Joaquin Valley ◽  
Resistance Response ◽  
Different Response ◽  
Race 4

Fusarium oxysporum f. sp. vasinfectum (FOV) race 4, is a causal agent of Fusarium wilt of cotton (Gossypium spp.). This study aimed to characterize the existing distribution and frequency of current field populations of FOV race 4 genotypes in the San Joaquin Valley (SJV) of California and Lower Valley El Paso, Texas and examine representative isolates for aggressiveness during different stages of seedling development. A survey was conducted from 2017 to 2019 across 13 locations in the SJV and one location in El Paso, Texas during 2018. From the SJV, isolates identified as the FOV race 4 T genotype were dispersed across the SJV, while isolates identified as the FOV race 4 N genotype were most frequently isolated from cotton fields in the northern county of Merced. The FOV race 4 isolates from the Texas location were identified as the MT genotype. A selection of representative isolates was evaluated using three inoculation assays, a rolled towel, FOV infested-oat seed, and a root dip inoculation assay to test the isolates’ abilities to produce symptoms during seedling stages of cotton development. All isolates tested were capable of producing symptoms on cotton, however isolate aggressiveness varied within and across inoculation assays. In all assays, higher levels of disease development were observed in the moderately susceptible Pima (G. barbadense L.) cultivars (DP-340 or PYH-830) when compared to the moderately tolerant Upland (G. hirsutum L.) cultivar (FM-2334). However, no correlation was found among the different response variables for the rolled towel assay when compared with the root dip and infested-oat seed assays. These results suggest that different genes are involved in the resistance response during the early seedling development stage measured in the rolled towel assay compared to the later seedling development stages measured during the root dip inoculation and infested-oat seed assays, revealing the complexity of the Fusarium wilt disease and host-plant resistance mechanisms.

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