scholarly journals Bioethanol Production from Cellulose-Rich Corncob Residue by the Thermotolerant Saccharomyces cerevisiae TC-5

2021 ◽  
Vol 7 (7) ◽  
pp. 547
Author(s):  
Pinpanit Boonchuay ◽  
Charin Techapun ◽  
Noppol Leksawasdi ◽  
Phisit Seesuriyachan ◽  
Prasert Hanmoungjai ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Elevated Temperatures ◽  
Ethanol Concentration ◽  
Ethanol Productivity ◽  
Solid Loading ◽  
Bioethanol Production ◽  
Fed Batch ◽  
Separate Hydrolysis And Fermentation ◽  
Ssf Process ◽  
Simultaneous Saccharification

This study aimed to select thermotolerant yeast for bioethanol production from cellulose-rich corncob (CRC) residue. An effective yeast strain was identified as Saccharomyces cerevisiae TC-5. Bioethanol production from CRC residue via separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and prehydrolysis-SSF (pre-SSF) using this strain were examined at 35–42 °C compared with the use of commercial S. cerevisiae. Temperatures up to 40 °C did not affect ethanol production by TC-5. The ethanol concentration obtained via the commercial S. cerevisiae decreased with increasing temperatures. The highest bioethanol concentrations obtained via SHF, SSF, and pre-SSF at 35–40 °C of strain TC-5 were not significantly different (20.13–21.64 g/L). The SSF process, with the highest ethanol productivity (0.291 g/L/h), was chosen to study the effect of solid loading at 40 °C. A CRC level of 12.5% (w/v) via fed-batch SSF resulted in the highest ethanol concentrations of 38.23 g/L. Thereafter, bioethanol production via fed-batch SSF with 12.5% (w/v) CRC was performed in 5-L bioreactor. The maximum ethanol concentration and ethanol productivity values were 31.96 g/L and 0.222 g/L/h, respectively. The thermotolerant S. cerevisiae TC-5 is promising yeast for bioethanol production under elevated temperatures via SSF and the use of second-generation substrates.

scholarly journals A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation of corn meal

2008 ◽  
Vol 14 (4) ◽  
pp. 231-234 ◽  
Author(s):  
Svetlana Nikolic ◽  
Ljiljana Mojovic ◽  
Marica Rakin ◽  
Dusanka Pejin ◽  
Dragisa Savic
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Concentration ◽  
Microwave Treatment ◽  
Ethanol Productivity ◽  
Bioethanol Production ◽  
Corn Meal ◽  
Microwave Assisted ◽  
Batch System ◽  
Ssf Process ◽  
Simultaneous Saccharification

A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation (SSF) of corn meal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. An optimal power of microwaves of 80 W and the 5-min duration of the microwave treatment were selected by following the concentration of glucose released from the corn meal suspensions at hidromodul of 1:3 (corn meal to water ratio) in the liquefaction step. The results indicated that the microwave pretreatment could increase the maximum ethanol concentration produced in the SSF process for 13.4 %. Consequently, a significant increase of the ethanol productivity on substrate (YP/S), as well as the volumetric ethanol productivity (P) in this process, could be achieved.

scholarly journals Bioethanol Production from Vineyard Waste by Autohydrolysis Pretreatment and Chlorite Delignification via Simultaneous Saccharification and Fermentation

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2606
Author(s):  
Lacrimioara Senila ◽  
Eniko Kovacs ◽  
Daniela Alexandra Scurtu ◽  
Oana Cadar ◽  
Anca Becze ◽  
...  
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Degradation Products ◽  
Liquid Fraction ◽  
Operating Conditions ◽  
Solid Loading ◽  
Sodium Chlorite ◽  
Chemical Compositions ◽  
Bioethanol Production ◽  
Ssf Process ◽  
Simultaneous Saccharification

In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF). The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol. The autohydrolysis of the vine-shoot wastes was carried out at 165 and 180 °C for 10 min residence time, and the resulted solid and liquid phases composition were analysed. The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products. The solid fraction was delignified using the sodium chlorite method for the separation of lignin and easier access of enzymes to the cellulosic sugars, and then, converted to ethanol by the SSF process. The maximum bioethanol production (6%) was obtained by autohydrolysis (165 °C), chlorite delignification and SSF process at 37 °C, 10% solid loading, 72 h. The principal component analysis was used to identify the main parameters that influence the chemical compositions of vine-shoot waste for different varieties.

scholarly journals Bioethanol Production from Several Tropical Wood Species using Simultaneous Saccharification and Fermentation Processes

2017 ◽  
Vol 4 (1) ◽  
pp. 106-116
Author(s):  
M. Daud ◽  
Wasrin Syafii ◽  
Khaswar Syamsu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Wood Species ◽  
Simultaneous Saccharification And Fermentation ◽  
Dry Mass ◽  
Bioethanol Production ◽  
Yeast Saccharomyces Cerevisiae ◽  
Tropical Wood ◽  
Kraft Process ◽  
Ssf Process ◽  
Simultaneous Saccharification

The study was conducted to determine the best method of hydrolysis (saccharification) and fermentation for bioethanol production using simultaneous saccharification and fermentation process. Three different tropical wood species namely gmelina wood (Gmelina sp.), pine wood (Pinus merkusii) and oil palm (Elaeis guineensis Jacq.) were pre-treated using kraft process and then converted into bioethanol using simultaneous saccharification and fermentation (SSF) processes. The pulp produced by kraft process was analized to determine their chemical properties before treatments. SSF was performed in 500 ml fermentors with total slurry of 200 ml. The substrate and nutrient media were autoclaved (121ºC and 20 min). The samples diluted to 2.5% (w/v) of total slurry were used as substrate. The enzyme preparation used commercial cellulase enzyme. The amount of cellulase added was 4 and 8% (w/w) of dry mass of samples. All SSF process was conducted by inoculating yeast Saccharomyces cerevisiae into fermentor in the amount of 10% (v/v) 1.5 x 109 CFU/cc. The SSF experiments run for 96 h, and the data were investigated periodically every 24 h. The results showed total sugar and reducing sugar tended to decrease with time of inoculation whereas ethanol concentration increases significantly. The growth of yeast Saccharomyces cerevisiae tended to incease in initial inoculation and decrease by the end of inoculation. The best method of hydrolysis (saccharification) and fermentation using SSF process for all tropical wood species tested were using cellulase 8% of dry mass (DM) and 10% (v/v) of Saccharomyces cerevisiae which produced bioethanol with concentration of 0.98; 0.57 and 0.51% for gmelina, pine and oil palm respectively and produced yields 11.21, 5.85 and 3.20%, in that order. 

scholarly journals High-Solid Loading Enzymatic Hydrolysis of Waste Office Paper for Poly-3-Hydroxybutyrate Production Through Simultaneous Saccharification and Fermentation

2021 ◽  
Author(s):  
Huda Al-Battashi ◽  
Nallusamy Sivakumar
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Waste Paper ◽  
Batch Experiment ◽  
Solid Loading ◽  
Fed Batch ◽  
Fixed Amount ◽  
Characteristic Features ◽  
Ssf Process ◽  
Simultaneous Saccharification ◽  
Office Paper

Abstract Waste paper holds great potential as a substrate for the microbial production of bioplastic (Poly-3-hydroxybutyrate (PHB)). This study aimed to produce PHB by utilizing office paper as a substrate using Cupriavidus necator through batch and fed-batch simultaneous saccharification and fermentation (SSF) approach. For the batch experiment, different loadings of shredded office paper (3, 5 and 10%) with two different pretreatments H2O2 (OPH) and H2O2 and Triton X-100 (OPTH) were carried out. For the fed-batch experiment, paper loading started with 3% and two more additions were made at 36 and 84 h. Both experiments were conducted at 30°C, 200 rpm and pH 7 using 55.5 FPU/g of cellulase and 37.5 CBU/g of β-glucosidase with a fixed amount of nitrogen source. High PHB yield was observed with OPH in all loadings, though the OPHT showed a better hydrolysis. Maximum PHB yield (4.27 g/L) was achieved with 10% OP at six days of fermentation in batch SSF. Whereas, maximum PHB yield (4.19 g/L) was obtained within a shorter time (66 h) in the fed-batch OPH paper. The extracted PHB showed well-matched characteristic features to the standard PHB. Finally, this study proves the feasibility of employing SSF process for PHB production using waste paper as an alternative approach to overcome the shortcoming of the separate hydrolysis and fermentation (SHF) process.

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