scholarly journals A Simple and Low-Cost Strategy to Improve Conidial Yield and Stress Resistance of Trichoderma guizhouense through Optimizing Illumination Conditions

2022 ◽  
Vol 8 (1) ◽  
pp. 50
Author(s):  
Yifan Li ◽  
Xiya Meng ◽  
Degang Guo ◽  
Jia Gao ◽  
Qiwei Huang ◽  
...  
Keyword(s):  
Blue Light ◽  
Stress Responses ◽  
Stress Resistance ◽  
Low Cost ◽  
Hog Pathway ◽  
Precise Control ◽  
Blue Light Receptor ◽  
Expression Of Genes ◽  
Low Cost Strategy ◽  
Stress Related Genes

Light is perceived by photoreceptors in fungi and further integrated into the stress-activated MAPK HOG pathway, and thereby potentially activates the expression of genes for stress responses. This indicates that the precise control of light conditions can likely improve the conidial yield and stress resistance to guarantee the low cost and long shelf life of Trichoderma-based biocontrol agents and biofertilizers. In this study, effects of wavelengths and intensities of light on conidial yield and stress tolerance to osmotic, oxidative and pH stresses in Trichoderma guizhouense were investigated. We found that 2 μmol photons/(m2 × s) of blue light increased the conidial yield more than 1000 folds as compared to dark condition and simultaneously enhanced conidial stress resistance. The enhanced conidial stress resistance is probably due to the upregulated stress-related genes in blue light, which is under the control of the blue light receptor BLR1 and the MAP kinase HOG1.

scholarly journals rpoS Mutations and Loss of General Stress Resistance in Escherichia coli Populations as a Consequence of Conflict between Competing Stress Responses

2002 ◽  
Vol 184 (3) ◽  
pp. 806-811 ◽  
Author(s):  
Lucinda Notley-McRobb ◽  
Thea King ◽  
Thomas Ferenci
Keyword(s):  
Escherichia Coli ◽  
Nutrient Limitation ◽  
Stress Responses ◽  
Stress Resistance ◽  
Acid Stress ◽  
Selective Advantage ◽  
General Stress ◽  
Expression Of Genes ◽  
Laboratory Populations ◽  
External Stresses

ABSTRACT The general stress resistance of Escherichia coli is controlled by the RpoS sigma factor (φS), but mutations in rpoS are surprisingly common in natural and laboratory populations. Evidence for the selective advantage of losing rpoS was obtained from experiments with nutrient-limited bacteria at different growth rates. Wild-type bacteria were rapidly displaced by rpoS mutants in both glucose- and nitrogen-limited chemostat populations. Nutrient limitation led to selection and sweeps of rpoS null mutations and loss of general stress resistance. The rate of takeover by rpoS mutants was most rapid (within 10 generations of culture) in slower-growing populations that initially express higher φS levels. Competition for core RNA polymerase is the likeliest explanation for reduced expression from distinct promoters dependent on φ70 and involved in the hunger response to nutrient limitation. Indeed, the mutation of rpoS led to significantly higher expression of genes contributing to the high-affinity glucose scavenging system required for the hunger response. Hence, rpoS polymorphism in E. coli populations may be viewed as the result of competition between the hunger response, which requires sigma factors other than φS for expression, and the maintenance of the ability to withstand external stresses. The extent of external stress significantly influences the spread of rpoS mutations. When acid stress was simultaneously applied to glucose-limited cultures, both the phenotype and frequency of rpoS mutations were attenuated in line with the level of stress. The conflict between the hunger response and maintenance of stress resistance is a potential weakness in bacterial regulation.

scholarly journals Initial Description of the Genome of Aeluropus Littoralis, a Halophile Grass

2020 ◽  
Author(s):  
Seyyed Hamidreza Hashemi ◽  
Mojhdeh Arab ◽  
Behnaz Dolatabadi ◽  
Yi-Tzu Kuo ◽  
Mariana Alejandra Baez ◽  
...  
Keyword(s):  
Stress Responses ◽  
Stress Resistance ◽  
Molecular Mechanisms ◽  
Expressed Sequence Tag ◽  
Salt Water ◽  
Crop Plants ◽  
Wild Plant ◽  
Salt Glands ◽  
Aeluropus Littoralis ◽  
Stress Related Genes

Abstract Background: The use of wild plant species or their halophytic relatives has been considered in plant breeding programs to improve salt and drought tolerance in crop plants. Aeluropus littoralis serves as halophyte model for identification and isolation of novel stress adaptation genes. This species is described as perennial monocot grass. A. littoralis grows in damp or arid areas, often salt-impregnated places and waste land in cultivated areas. A. littoralis can survive where the water salinity is periodically high and tolerate high salt concentrations in the soil up to 1100 mM sodium chloride. Therefore, it serves as valuable genetic resource to understand molecular mechanisms of stress-responses in monocots. The knowledge can potentially be used for improving tolerance to abiotic stresses in economically important crops. Several morphological, anatomical, ecological, and physiological traits of A. littoralis have been investigated so far and also the transfer of stress related genes to other species resulted in enhanced stress resistance. After watering with salt water the grass is able to excrete salt via its salt glands. Meanwhile, a number of ESTs (expressed sequence tag), genes and promoters induced by the salt and drought stresses were isolated, sequenced and annotated at a molecular level. Results: Here we describe the genome sequence and structure of A. littoralis analyzed by whole genome sequencing and histological analysis. The chromosome number was determined to be 20 (2n = 2X = 20), absence of B chromsomes shown, and the genome size calculated to be 354 Megabasepairs.Conclusions: This genomic information provided here, will support the functional investigation and application of novel genes improving salt stress resistance in crop plants.

scholarly journals Initial description of the Genome of Aeluropus littoralis, a halophile grass

2020 ◽  
Author(s):  
Seyyed Hamidreza Hashemi ◽  
Mojhdeh Arab ◽  
Behnaz Dolatabadi ◽  
Yi-Tzu Kuo ◽  
Mariana Alejandra Baez ◽  
...  
Keyword(s):  
Stress Responses ◽  
Stress Resistance ◽  
Molecular Mechanisms ◽  
Expressed Sequence Tag ◽  
Salt Water ◽  
Crop Plants ◽  
Wild Plant ◽  
Salt Glands ◽  
Aeluropus Littoralis ◽  
Stress Related Genes

Abstract Background: The use of wild plant species or their halophytic relatives has been considered in plant breeding programs to improve salt and drought tolerance in crop plants. Aeluropus littoralis serves as halophyte model for identification and isolation of novel stress adaptation genes. This species is described as perennial monocot grass. A. littoralis grows in damp or arid areas, often salt-impregnated places and waste land in cultivated areas. A. littoralis can survive where the water salinity is periodically high and tolerate high salt concentrations in the soil up to 1100 mM sodium chloride. Therefore, it serves as valuable genetic resource to understand molecular mechanisms of stress-responses in monocots. The knowledge can potentially be used for improving tolerance to abiotic stresses in economically important crops. Several morphological, anatomical, ecological, and physiological traits of A. littoralis have been investigated so far and also the transfer of stress related genes to other species resulted in enhanced stress resistance. After watering with salt water the grass is able to excrete salt via its salt glands. Meanwhile, a number of ESTs (expressed sequence tag), genes and promoters induced by the salt and drought stresses were isolated, sequenced and annotated at a molecular level.Results: Here we describe the genome sequence and structure of A. littoralis analyzed by whole genome sequencing and histological analysis. The chromosome number was determined to be 20 (2n = 2X = 20), absence of B chromsomes shown, and the genome size calculated to be 354 Megabasepairs.Conclusions: This genomic information provided here, will support the functional investigation and application of novel genes improving salt stress resistance in crop plants.

Investigation of in-feed organic acids as a low cost strategy to combat Salmonella in grower pigs

2017 ◽  
Vol 139 ◽  
pp. 50-57 ◽  
Author(s):  
H. Lynch ◽  
F.C. Leonard ◽  
K. Walia ◽  
P.G. Lawlor ◽  
G. Duffy ◽  
...  
Keyword(s):  
Organic Acids ◽  
Low Cost ◽  
Cost Strategy ◽  
Low Cost Strategy

Measuring Optical Properties of Advanced Nano/Micro-Periodic Structures Fabricated by Multi-Exposure Interference Lithography

2020 ◽  
Author(s):  
Shuzo Masui ◽  
Masaki Michihata ◽  
Kiyoshi Takamasu ◽  
Satoru Takahashi
Keyword(s):  
Periodic Structures ◽  
Low Cost ◽  
Industrial Applications ◽  
Interference Lithography ◽  
High Dispersion ◽  
Optical Elements ◽  
Precise Control ◽  
Periodic Grating ◽  
Interference Fringes ◽  
Multiple Interference

Abstract Functional optical elements based on nano/micro-periodic structures have attracted much attention. Since the fabrication of these dual-periodic structures requires precise control of periodicity, the semiconductor process such as an electron beam lithography has been mainly employed. However, these techniques have problems with expensive and low throughput for industrial applications. Therefore, there remains a need for low cost and high throughput fabrication methods of dual-periodic structures. Then we developed a multi-exposure interference lithography (MEIL) system using rotational Lloyd’s mirror interferometer to overcome these problems. The advantages of interference lithography are a large processing area and low cost. Our developed rotational Lloyd’s mirror setup enables us to a highly precise superposition of multiple interference fringes by multi-exposure. Furthermore, we developed a measurement setup for reflective diffractive elements using a two axial rotating stage and measured the diffraction properties of the fabricated dual-periodic diffraction gratings. In this paper, as a demonstration, we succeeded in the fabrication of high-dispersion diffraction grating with an enhanced diffraction efficiency of the −3rd order light. The fabricated shapes have a periodicity of 1997 nm and 665 nm. Furthermore, it was confirmed that the intensity of the −3rd order light was enhanced by about 10 times compared to the single periodic grating.

Light-dependent suppression of COP1 multimeric complex formation is determined by the blue-light receptor FKF1 in Arabidopsis

2019 ◽  
Vol 508 (1) ◽  
pp. 191-197 ◽  
Author(s):  
Byoung-Doo Lee ◽  
Joon-Yung Cha ◽  
Mi Ri Kim ◽  
Gyeong-Im Shin ◽  
Nam-Chon Paek ◽  
...  
Keyword(s):  
Complex Formation ◽  
Blue Light ◽  
Blue Light Receptor ◽  
Multimeric Complex ◽  
Light Receptor

scholarly journals Performance of the dynamically dimensioned search algorithm: influence of parameter initialization strategy when calibrating a physically based hydrological model

2017 ◽  
Vol 49 (4) ◽  
pp. 971-988 ◽  
Author(s):  
Franck Lespinas ◽  
Ashu Dastoor ◽  
Vincent Fortin
Keyword(s):  
Search Algorithm ◽  
Low Cost ◽  
Initial Parameter ◽  
Cost Strategy ◽  
Physically Based ◽  
Physically Based Models ◽  
Calibration Solution ◽  
Low Cost Strategy ◽  
Parameter Values ◽  
Initialization Strategy

Abstract This study presents an evaluation of the performance of the dynamically dimensioned search (DDS) algorithm when calibrating the hydrological component of the Visualizing Ecosystems for Land Management Assessments (VELMA) ecohydrological model. Two calibration strategies were tested for the initial parameter values: (1) a ‘high-cost strategy’, where 100 sets of initial parameter values were randomly chosen within the overall parameter space, and (2) a ‘low-cost strategy’, where a unique set of initial parameter values was derived from the available field data. Both strategies were tested for six different values of the maximum number of model evaluations ranging between 100 and 10,000. Results revealed that DDS is able to converge rapidly to a good parameter calibration solution of the VELMA hydrological component regardless of the parameter initialization strategy used. The accuracy and convergence efficiency of the DDS algorithm were, however, slightly better for the low-cost strategy. This study suggests that initializing the parameter values of complex physically based models using information on the watershed characteristics can increase the efficiency of the automatic calibration procedures.

scholarly journals Waste Conversion into a Sweetener—Development of an Innovative Strategy for Erythritol Production by Yarrowia lipolytica

2020 ◽  
Vol 12 (17) ◽  
pp. 7122
Author(s):  
Ludwika Tomaszewska-Hetman ◽  
Waldemar Rymowicz ◽  
Anita Rywińska
Keyword(s):  
Yarrowia Lipolytica ◽  
Crude Glycerol ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Culture Broth ◽  
Biodiesel Production ◽  
Innovative Strategy ◽  
Simple Medium ◽  
Low Cost Strategy ◽  
Steep Liquor

The study proposed the innovative low-cost strategy for erythritol production by Yarrowia lipolytica through developing a simple medium based on industrial waste by-products and a natural method for culture broth purification. Results obtained proved that corn steep liquor might successfully replace traditional sources of nitrogen and other nutrients without compromising activities of the enzymes responsible for erythritol production and its production level. As a consequence, a production process was performed where Y. lipolytica A-6 was able to produce 108.0 g/L of erythritol, with a production rate of 1.04 g/Lh and a yield of 0.45 g/g of the medium containing exclusively 220 g/L of crude glycerol derived from biodiesel production and 40 g/L of corn steep liquor. Moreover, a comparable concentration of erythritol (108.1 g/L) was obtained when a part of crude glycerol was exchanged for the crude fraction of fatty acids in the two-steps process. Next, the collected post-fermentation broths were used in the culture with Y. lipolytica Wratislavia K1 for natural purification. The process resulted in a high increase of erythritol selectivity from 72% to 97% and in the production of 22.0 g/L of biomass with 40.4% protein content, which enables its use as an attractive animal feedstuff.

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