Forsythoside A Alleviates Imiquimod-Induced Psoriasis-like Dermatitis in Mice by Regulating Th17 Cells and IL-17A Expression

Psoriasis is a recurrent inflammatory skin disease characterized by redness and scaly skin lesions with itchy or painful sensations. Forsythoside A, one of the main active compounds isolated from the fruit of Forsythia suspensa, has been widely applied to treat inflammatory diseases in the clinical use of traditional oriental medicine. However, the effect of forsythoside A on psoriasis remains unclear. This study aimed to explore the therapeutic effects and immune regulation of forsythoside A on psoriasis. C57BL/6 mice were divided into six groups and treated with imiquimod cream on their shaved back skin to induce psoriasis-like dermatitis. Different doses of forsythoside A (5 mg/kg, 10 mg/kg, or 20 mg/kg) were administered to the respective treatment groups. Skin redness, scaling, and ear thickness were measured; keratinocyte proliferation and inflammatory cytokine expression were detected by hematoxylin–eosin and immunohistochemical staining. Th17 cells in the inguinal lymph nodes were detected by flow cytometric analysis. IL-17A levels were measured using ELISA. The results showed that forsythoside A relieved psoriatic skin symptoms such as skin redness, thickness, scaling, and reduced epidermal thickening. The expression of IL-6, IL-17, and Ki-67 was downregulated in the forsythoside-A-treated groups. Th17 cell expression in inguinal lymph nodes and IL-17A secretion was suppressed by forsythoside A. In conclusion, forsythoside A was found to alleviate imiquimod-induced psoriasis-like dermatitis in mice by suppressing Th17 development and IL-17A secretion. These findings demonstrate the feasibility of forsythoside A in treating human psoriasis.

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Morphological characteristic of inguinal lymph nodes in HIV infection

Perm Medical Journal ◽

10.17816/pmj36251-59 ◽

2019 ◽

Vol 36 (2) ◽

pp. 51-59

Author(s):

N. I. Gulyaeva ◽

G. G. Freind ◽

N. G. Shmagel ◽

L. B. Korolevskaya ◽

E. V. Saidakova ◽

...

Keyword(s):

Lymph Node ◽

Antiretroviral Therapy ◽

T Lymphocytes ◽

Hiv Infection ◽

Lymph Nodes ◽

Blood Lymphocyte ◽

Ki 67 ◽

Inguinal Lymph Nodes ◽

Group I ◽

Lymphocyte Number

Aim. To assess the morphological changes in inguinal lymph nodes among patients with stage 4a HIV infection, who receive antiretroviral therapy. Materials and methods. There were examined 12 HIV-infected patients with stage 4a, treated at “Perm Regional Center for Prevention and Fight against AIDS and Infectious Diseases”, who received antiretroviral therapy for 2 years. Two groups of patients were formed: group I – 6 persons, whose CD+ blood lymphocyte number was more than 350 in 1 mcl; group II – 6 persons with CD+ blood lymphocyte number less than 350 in 1 mcl. Inguinal lymph nodes (ILN) were taken under local anesthesia, histological preparations were prepared by traditional scheme, stained with hematoxylin and eosin using Masson three-colour staining. In immunohistochemical reactions expression of Ki-67 and CD+ markers was estimated. Results.Histoarchitectonics of lymph nodes was changed as a result of massive development of sclerosis in the region of hilum, capsule and trabecules. Against the background of sclerosis, there occurred lymphocyte depletion, change in structure of stromal cells and neoangiogenesis in all the zones of the lymph node. In the regions of sclerosis, death of lymphocytes was revealed. In patients of the second group, more active development of follicles with the centers of reproduction in the lymph node cortical substance, as well as growth of the number of Ki-67 maker-expressing cells was established Conclusions.In the inguinal lymph nodes, the development of sclerotic processes causes the death of T-lymphocytes, which, in their turn, are the source of lymphotoxin-β formation. Loss of CD+-T-lymphocytes is accompanied by deficit of lymphotoxin-β and induces the loss of fibroblastic reticular cells themselves, which through the production of IL-7 support the vital activity of T-cells.

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Radiation synovectomy of the knee joint

Nuklearmedizin ◽

10.1055/s-0038-1623935 ◽

2006 ◽

Vol 45 (01) ◽

pp. 57-61

Author(s):

M. Puille ◽

D. Steiner ◽

R. Bauer ◽

R. Klett

Keyword(s):

Knee Joint ◽

Lymph Nodes ◽

Geometric Mean ◽

Radiation Synovectomy ◽

Real Activity ◽

Anterior View ◽

Inguinal Lymph Nodes ◽

The Real ◽

Therapeutic Modalities ◽

Real Value

Summary Aim: Multiple procedures for the quantification of activity leakage in radiation synovectomy of the knee joint have been described in the literature. We compared these procedures considering the real conditions of dispersion and absorption using a corpse phantom. Methods: We simulated different distributions of the activity in the knee joint and a different extra-articular spread into the inguinal lymph nodes. The activity was measured with a gammacamera. Activity leakage was calculated by measuring the retention in the knee joint only using an anterior view, using the geometric mean of anterior and posterior views, or using the sum of anterior and posterior views. The same procedures were used to quantify the activity leakage by measuring the activity spread into the inguinal lymph nodes. In addition, the influence of scattered rays was evaluated. Results: For several procedures we found an excellent association with the real activity leakage, shown by an r² between 0.97 and 0.98. When the real value of the leakage is needed, e. g. in dosimetric studies, simultaneously measuring of knee activity and activity in the inguinal lymph nodes in anterior and posterior views and calculation of the geometric mean with exclusion of the scatter rays was found to be the procedure of choice. Conclusion: When measuring of activity leakage is used for dosimetric calculations, the above-described procedure should be used. When the real value of the leakage is not necessary, e. g. for comparing different therapeutic modalities, several of the procedures can be considered as being equivalent.

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Faculty Opinions recommendation of Expansion of Th17 cells and functional defects in T regulatory cells are key features of the pancreatic lymph nodes in patients with type 1 diabetes.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717955689.793460701 ◽

2012 ◽

Author(s):

Mark Peakman

Keyword(s):

Type 1 Diabetes ◽

Lymph Nodes ◽

Th17 Cells ◽

T Regulatory Cells ◽

Regulatory Cells ◽

Pancreatic Lymph Nodes ◽

Key Features ◽

Functional Defects

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A proposed Clino-radio-pathological Risk Scoring System (CRiSS) for prediction and management of inguinal lymph-nodes metastasis in squamous cell carcinoma of the penis

Surgical Oncology ◽

10.1016/j.suronc.2020.12.010 ◽

2021 ◽

Vol 36 ◽

pp. 147-152

Author(s):

Keval N. Patel ◽

Chirag Bhirud ◽

J. Dipin ◽

Kunal Nandy ◽

Vivek Venugopal ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Lymph Nodes ◽

Cell Carcinoma ◽

Squamous Cell ◽

Scoring System ◽

Inguinal Lymph Nodes ◽

Lymph Nodes Metastasis ◽

Risk Scoring ◽

Risk Scoring System

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Prognostic Factors in Patients With Melanoma Metastatic to Axillary or Inguinal Lymph Nodes

Annals of Surgery ◽

10.1097/00000658-199111000-00014 ◽

1991 ◽

Vol 214 (5) ◽

pp. 627-636 ◽

Cited By ~ 124

Author(s):

DANIEL G. COIT ◽

ANDRE ROGATKO ◽

MURRAY F. BRENNAN

Keyword(s):

Prognostic Factors ◽

Lymph Nodes ◽

Inguinal Lymph Nodes

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Subcutaneous sacrococcygeal ependymoma with inguinal lymph node metastastis

Journal of Neurosurgery ◽

10.3171/jns.1988.68.3.0474 ◽

1988 ◽

Vol 68 (3) ◽

pp. 474-477 ◽

Cited By ~ 23

Author(s):

G. W. P. M. Kramer ◽

E. Rutten ◽

J. Sloof

Keyword(s):

Lymph Node ◽

Lymph Nodes ◽

Inguinal Lymph Node ◽

Content Type ◽

Inguinal Lymph Nodes ◽

Fine Print

✓ A patient with a subcutaneous sacrococcygeal ependymoma and metastasis to the inguinal lymph nodes is presented and his treatment is described. Previous reports on sacrococcygeal ependymoma are reviewed.

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Mesenchymal stem cells induce the expansion of regulatory T cells in abortion-prone mice

Reproduction ◽

10.1530/rep-20-0320 ◽

2021 ◽

Author(s):

Amir Salek Farrokhi ◽

Amir-Hassan Zarnani ◽

Fatemeh Rezaei kahmini ◽

Seyed Mohammad Moazzeni

Keyword(s):

Stem Cells ◽

T Cells ◽

Mesenchymal Stem Cells ◽

Regulatory T Cells ◽

Immune Responses ◽

Lymph Nodes ◽

De Novo ◽

Abortion Rate ◽

Cellular Mechanisms ◽

Inguinal Lymph Nodes

Recurrent pregnancy loss (RPL) is one of the most common complications of early pregnancy associated in most cases with local or systemic immune abnormalities such as the diminished proportion of regulatory T cells (Tregs). Mesenchymal stem cells (MSCs) have been shown to modulate immune responses by de novo induction and expansion of Tregs. In this study, we analyzed the molecular and cellular mechanisms involved in Treg-associated pregnancy protection following MSCs administration in an abortion-prone mouse mating. In a case-control study, syngeneic abdominal fat-derived MSCs were administered intraperitoneally (i.p) to the DBA/2-mated CBA/J female mice on day 4.5 of pregnancy. Abortion rate, Tregs proportion in spleen and inguinal lymph nodes, and Ho1, Foxp3, Pd1, and Ctla4 genes expression at the feto-maternal interface were then measured on day 13.5 of pregnancy using flow cytometry and quantitative RT- PCR, respectively. The abortion rate in MSCs-treated mice was significantly reduced and normalized to the level observed in normal pregnant animals. We demonstrated a significant induction of Tregs in inguinal lymph nodes but not in the spleen following MSCs administration. Administration of MSCs remarkably upregulated the expression of HO1, Foxp3, Pd1, and Ctla4 genes in both placenta and decidua. Here, we show that MSCs therapy could protect the fetus in the abortion-prone mice through Tregs expansion and up-regulation of Treg-related genes. These events could establish an immune-privileged microenvironment, which participates in regulation of detrimental maternal immune responses against the semi-allogeneic fetus.

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