scholarly journals The Mitochondrial Genome of a Plant Fungal Pathogen Pseudocercospora fijiensis (Mycosphaerellaceae), Comparative Analysis and Diversification Times of the Sigatoka Disease Complex Using Fossil Calibrated Phylogenies

Life ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 215
Author(s):  
Juliana E. Arcila-Galvis ◽  
Rafael E. Arango ◽  
Javier M. Torres-Bonilla ◽  
Tatiana Arias
Keyword(s):  
Comparative Analysis ◽  
Mitochondrial Genome ◽  
Sister Group ◽  
Time Frame ◽  
Sister Species ◽  
Rrna Genes ◽  
Disease Complex ◽  
Transport Chain ◽  
Dated Phylogeny ◽  
Pseudocercospora Fijiensis

Mycosphaerellaceae is a highly diverse fungal family containing a variety of pathogens affecting many economically important crops. Mitochondria play a crucial role in fungal metabolism and in the study of fungal evolution. This study aims to: (i) describe the mitochondrial genome of Pseudocercospora fijiensis, and (ii) compare it with closely related species (Sphaerulina musiva, S. populicola, P. musae and P. eumusae) available online, paying particular attention to the Sigatoka disease’s complex causal agents. The mitochondrial genome of P. fijiensis is a circular molecule of 74,089 bp containing typical genes coding for the 14 proteins related to oxidative phosphorylation, 2 rRNA genes and a set of 38 tRNAs. P. fijiensis mitogenome has two truncated cox1 copies, and bicistronic transcription of nad2-nad3 and atp6-atp8 confirmed experimentally. Comparative analysis revealed high variability in size and gene order among selected Mycosphaerellaceae mitogenomes likely to be due to rearrangements caused by mobile intron invasion. Using fossil calibrated Bayesian phylogenies, we found later diversification times for Mycosphaerellaceae (66.6 MYA) and the Sigatoka disease complex causal agents, compared to previous strict molecular clock studies. An early divergent Pseudocercospora fijiensis split from the sister species P. musae + P. eumusae 13.31 MYA while their sister group, the sister species P. eumusae and P. musae, split from their shared common ancestor in the late Miocene 8.22 MYA. This newly dated phylogeny suggests that species belonging to the Sigatoka disease complex originated after wild relatives of domesticated bananas (section Eumusae; 27.9 MYA). During this time frame, mitochondrial genomes expanded significantly, possibly due to invasions of introns into different electron transport chain genes.

scholarly journals The complete mitochondrial genome sequence of Scolopendra mutilans L. Koch, 1878 (Scolopendromorpha, Scolopendridae), with a comparative analysis of other centipede genomes

ZooKeys ◽  
2020 ◽  
Vol 925 ◽  
pp. 73-88
Author(s):  
Chaoyi Hu ◽  
Shuaibin Wang ◽  
Bisheng Huang ◽  
Hegang Liu ◽  
Lei Xu ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Complete Mitochondrial Genome ◽  
Gc Content ◽  
Sister Group ◽  
Rrna Genes ◽  
Sister Group Relationship ◽  
Trna Genes ◽  
Protein Coding ◽  
Generation Sequencing ◽  
Simple Sequence

Scolopendra mutilans L. Koch, 1878 is an important Chinese animal with thousands of years of medicinal history. However, the genomic information of this species is limited, which hinders its further application. Here, the complete mitochondrial genome (mitogenome) of S. mutilans was sequenced and assembled by next-generation sequencing. The genome is 15,011 bp in length, consisting of 13 protein-coding genes (PCGs), 14 tRNA genes, and two rRNA genes. Most PCGs start with the ATN initiation codon, and all PCGs have the conventional stop codons TAA and TAG. The S. mutilans mitogenome revealed nine simple sequence repeats (SSRs), and an obviously lower GC content compared with other seven centipede mitogenomes previously sequenced. After analysis of homologous regions between the eight centipede mitogenomes, the S. mutilans mitogenome further showed clear genomic rearrangements. The phylogenetic analysis of eight centipedes using 13 conserved PCG genes was finally performed. The phylogenetic reconstructions showed Scutigeromorpha as a separate group, and Scolopendromorpha in a sister-group relationship with Lithobiomorpha and Geophilomorpha. Collectively, the S. mutilans mitogenome provided new genomic resources, which will improve its medicinal research and applications in the future.

scholarly journals Assembly and comparative analysis of the first complete mitochondrial genome of Acer truncatum Bunge: a woody oil-tree species producing nervonic acid

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Qiuyue Ma ◽  
Yuxiao Wang ◽  
Shushun Li ◽  
Jing Wen ◽  
Lu Zhu ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Mitochondrial Genome ◽  
Tree Species ◽  
Complete Mitochondrial Genome ◽  
Taxonomic Status ◽  
Rrna Genes ◽  
Nervonic Acid ◽  
Trna Genes ◽  
Molecular Barcoding ◽  
Gene Migration

Abstract Background Acer truncatum (purpleblow maple) is a woody tree species that produces seeds with high levels of valuable fatty acids (especially nervonic acid). The species is admired as a landscape plant with high developmental prospects and scientific research value. The A. truncatum chloroplast genome has recently been reported; however, the mitochondrial genome (mitogenome) is still unexplored. Results We characterized the A. truncatum mitogenome, which was assembled using reads from PacBio and Illumina sequencing platforms, performed a comparative analysis against different species of Acer. The circular mitogenome of A. truncatum has a length of 791,052 bp, with a base composition of 27.11% A, 27.21% T, 22.79% G, and 22.89% C. The A. truncatum mitogenome contains 62 genes, including 35 protein-coding genes, 23 tRNA genes and 4 rRNA genes. We also examined codon usage, sequence repeats, RNA editing and selective pressure in the A. truncatum mitogenome. To determine the evolutionary and taxonomic status of A. truncatum, we conducted a phylogenetic analysis based on the mitogenomes of A. truncatum and 25 other taxa. In addition, the gene migration from chloroplast and nuclear genomes to the mitogenome were analyzed. Finally, we developed a novel NAD1 intron indel marker for distinguishing several Acer species. Conclusions In this study, we assembled and annotated the mitogenome of A. truncatum, a woody oil-tree species producing nervonic acid. The results of our analyses provide comprehensive information on the A. truncatum mitogenome, which would facilitate evolutionary research and molecular barcoding in Acer.

scholarly journals Assembly And Comparative Analysis of The First Complete Mitochondrial Genome of Acer Truncatum Bunge: A Woody Oil-Tree Species Producing Nervonic Acid

2021 ◽  
Author(s):  
Qiuyue Ma ◽  
Yuxiao Wang ◽  
Shushun Li ◽  
Jing Wen ◽  
Lu Zhu ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Mitochondrial Genome ◽  
Tree Species ◽  
Complete Mitochondrial Genome ◽  
Taxonomic Status ◽  
Rrna Genes ◽  
Nervonic Acid ◽  
Trna Genes ◽  
Molecular Barcoding ◽  
Gene Migration

Abstract Background: Acer truncatum (purpleblow maple) is a woody tree species that produces seeds with high levels of valuable fatty acids (especially nervonic acid). The species is also admired as a landscape plant with high developmental prospects and scientific research value. The A. truncatum chloroplast genome has recently been reported; however, the mitochondrial genome (mitogenome) is still unexplored.Results: We characterized the A. truncatum mitogenome, which was assembled using reads from Pacbio and Illumina sequencing platforms, and performed a comparative analysis against different species of Acer. The circular mitogenome of A. truncatum has a length of 791,052 bp, with a base composition of 27.11% A, 22.21% T, 22.79% G, and 22.89% C. The A. truncatum mitogenome contains 62 genes, including 35 protein-coding genes, 23 tRNA genes, and 4 rRNA genes. We also examined codon usage, sequence repeats, RNA editing, and selective pressure in the A. truncatum mitogenome. To determine the evolutionary and taxonomic status of A. truncatum, we conducted a phylogenetic analysis based on the mitogenomes of A. truncatum and 25 other taxa. We also analyzed gene migration from chloroplast and nuclear genomes to the mitogenome. Finally, we developed a novel NAD1 intron indel marker for distinguishing several Acer species. Conclusions: In this study, we assembled and annotated the mitogenome of A. truncatum, a woody oil-tree species producing nervonic acid. The results of our analyses provide comprehensive information on the A. truncatum mitogenome, which should facilitate evolutionary research and molecular barcoding in Acer.

scholarly journals The Mitochondrial Genome of the Hemichordate Balanoglossus carnosus and the Evolution of Deuterostome Mitochondria

Genetics ◽  
1998 ◽  
Vol 150 (3) ◽  
pp. 1115-1123
Author(s):  
Jose Castresana ◽  
Gertraud Feldmaier-Fuchs ◽  
Shin-ichi Yokobori ◽  
Noriyuki Satoh ◽  
Svante Pääbo
Keyword(s):  
Mitochondrial Genome ◽  
Phylogenetic Trees ◽  
Mitochondrial Gene ◽  
Sister Group ◽  
Rrna Genes ◽  
Gene Arrangement ◽  
Noncoding Regions ◽  
Transcriptional Promoters ◽  
Genes Encoding ◽  
Acorn Worm

Abstract The complete nucleotide sequence of the mitochondrial genome of the hemichordate Balanoglossus carnosus (acorn worm) was determined. The arrangement of the genes encoding 13 protein, 22 tRNA, and 2 rRNA genes is essentially the same as in vertebrates, indicating that the vertebrate and hemichordate mitochondrial gene arrangement is close to that of their common ancestor, and, thus, that it has been conserved for more than 600 million years, whereas that of echinoderms has been rearranged extensively. The genetic code of hemichordate mitochondria is similar to that of echinoderms in that ATA encodes isoleucine and AGA serine, whereas the codons AAA and AGG, whose amino acid assignments also differ between echinoderms and vertebrates, are absent from the B. carnosus mitochondrial genome. There are three noncoding regions of length 277, 41, and 32 bp: the larger one is likely to be equivalent to the control region of other deuterostomes, while the two others may contain transcriptional promoters for genes encoded on the minor coding strand. Phylogenetic trees estimated from the inferred protein sequences indicate that hemichordates are a sister group of echinoderms.

Systematic studies in Paniceae (Poaceae): Homopholis and Whalleya gen. et sp. nov.

2000 ◽  
Vol 13 (3) ◽  
pp. 437 ◽  
Author(s):  
Karen E. Wills ◽  
R. D. B. Whalley ◽  
Jeremy J. Bruhl
Keyword(s):  
Sister Group ◽  
The Other ◽  
Sister Species ◽  
Parsimonious Tree ◽  
The Third ◽  
Putative Species ◽  
Anatomical Data ◽  
Cladistic Analyses

The taxonomy ofHomopholis C.E.Hubb. is revised, and anew genus Whalleya K.E.Wills & J.J.Bruhl isdescribed. Relationships among the known species ofHomopholis (H. belsonii C.E.Hubb.,H. proluta F.Muell., and a putative species,H. sp. nov.), and the relationships betweenHomopholis and other genera within the Paniceae were investigated. Morphological and anatomical data forHomopholis and selected species ofDigitaria and Panicum were analysed phenetically and cladistically. The value and contribution ofcharacters to the findings were assessed. In the phenetic analyses, threedistinct clusters of species were formed. The first cluster includedDigitaria coenicola (F.Muell.) Hughes,D. divaricatissima (R.Br.) Hughes andD. papposa (R.Br.) P.Beauv.; the second,Panicum effusum R.Br.,P. queenslandicum Domin var.queenslandicum and P. simileDomin; and the third, H. sp. nov.,H. proluta, H. belsonii andP. subxerophilum Domin. Specimens ofH. belsonii noticeably separated from the other threespecies. For the cladistic analyses, species ofEntolasia and Thyridolepis were used as outgroup taxa. One most parsimonious tree was produced.Homopholis belsonii was well supported as the most basalmember of the ingroup. The three species ofDigitariaformed a well-supported clade.Panicum effusum, P. queenslandicumvar. queenslandicum and P. simileformed a well-supported clade, and were the sister group toEntolasia marginata (R.Br.) Hughes andE. stricta (R.Br.) Hughes.Panicum subxerophilum was in a clade (=Whalleya) with H. sp. nov. andH. proluta, with P. subxerophilumand H. proluta as sister species.

scholarly journals NAST: a multiple sequence alignment server for comparative analysis of 16S rRNA genes

2006 ◽  
Vol 34 (Web Server) ◽  
pp. W394-W399 ◽  
Author(s):  
T. Z. DeSantis ◽  
P. Hugenholtz ◽  
K. Keller ◽  
E. L. Brodie ◽  
N. Larsen ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
16S Rrna ◽  
Sequence Alignment ◽  
Multiple Sequence Alignment ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Multiple Sequence

scholarly journals Analysis of rail yard and terminal performances

2014 ◽  
Vol 8 (2) ◽  
pp. 178-200 ◽  
Author(s):  
Marin Marinov ◽  
Leonardo Di Giovanni ◽  
Giulia Bellisai ◽  
Julian Clevermann ◽  
Anastasia Mastellou ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Critical Points ◽  
Network Performance ◽  
Logical Consequence ◽  
Time Frame ◽  
Key Factors ◽  
Railway Network ◽  
Rail Infrastructure ◽  
Different Types ◽  
Junction Points

One of the latest trends in the transport field is the increasing interest for the rejuvenation of the railway. It is considered to be a logical consequence of the gradual switch towards a more sustainable future in transports. Terminals and stations are considered to be the junction points between the various lines that constitute the railway network and can simply be described as points of arrival, departure and interchange of passengers or commodities. The most commonly used indicators that measure the level of their performance are time and cost. This study aims at exploring possible improvements that could be implemented to the infrastructure and the operation of terminals and stations in order to increase the efficiency level. Firstly, drawing upon grounded theory about rail infrastructure and terminals, a description is conducted, followed by a comparative analysis of the different types of existing terminals and stations. Secondly, the suggested improvements are presented in accordance with their time frame completion. The main contribution of this study is to illustrate the high significance of terminals, stations and yards, acknowledging them as crucial parts of the railway network, because as characterized and demonstrated in this study, their performance are key factors to the whole network performance, making the identification of their critical points and respective possible solutions, the final objective of this paper. In addition to this, emphasis is given to the need of improving and developing the existing terminal infrastructure and operations.

scholarly journals Complete mitochondrial genome of Black Soft-shell Turtle (Nilssonia nigricans) and comparative analysis with other Trionychidae

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Shantanu Kundu ◽  
Vikas Kumar ◽  
Kaomud Tyagi ◽  
Rajasree Chakraborty ◽  
Devkant Singha ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Mitochondrial Genome ◽  
Complete Mitochondrial Genome ◽  
Soft Shell

scholarly journals The mitochondrial genome of Dipetalonema gracile from a squirrel monkey in China

2018 ◽  
Vol 94 ◽  
Author(s):  
P. Zhang ◽  
R.K. Ran ◽  
A.Y. Abdullahi ◽  
X.L. Shi ◽  
Y. Huang ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Squirrel Monkey ◽  
Complete Mitochondrial Genome ◽  
Mitochondrial Gene ◽  
Rrna Genes ◽  
Saimiri Sciureus ◽  
Trna Genes ◽  
Loop Structure ◽  
Protein Coding ◽  
Protein Coding Genes

AbstractDipetalonema gracile is a common parasite in squirrel monkeys (Saimiri sciureus), which can cause malnutrition and progressive wasting of the host, and lead to death in the case of massive infection. This study aimed to identify a suspected D. gracile worm from a dead squirrel monkey by means of molecular biology, and to amplify its complete mitochondrial genome by polymerase chain reaction (PCR) and sequence analysis. The results identified the worm as D. gracile, and the full length of its complete mitochondrial genome was 13,584 bp, which contained 22 tRNA genes, 12 protein-coding genes, two rRNA genes, one AT-rich region and one small non-coding region. The nucleotide composition included A (16.89%), G (20.19%), T (56.22%) and C (6.70%), among which A + T = 73.11%. The 12 protein-coding genes used TTG and ATT as start codons, and TAG and TAA as stop codons. Among the 22 tRNA genes, only trnS1AGN and trnS2UCN exhibited the TΨC-loop structure, while the other 20 tRNAs showed the TV-loop structure. The rrnL (986 bp) and rrnS (685 bp) genes were single-stranded and conserved in secondary structure. This study has enriched the mitochondrial gene database of Dipetalonema and laid a scientific basis for further study on classification, and genetic and evolutionary relationships of Dipetalonema nematodes.

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