scholarly journals Efficacy of Bioactive Glass Nanofibers Tested for Oral Mucosal Regeneration in Rabbits with Induced Diabetes

Materials ◽  
2020 ◽  
Vol 13 (11) ◽  
pp. 2603 ◽  
Author(s):  
Noha Elshazly ◽  
Abdelaziz Khalil ◽  
Manal Saad ◽  
Marco Patruno ◽  
Jui Chakraborty ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Bioactive Glass ◽  
Cell Activity ◽  
The Other ◽  
Control Group ◽  
Time Interval ◽  
Type I ◽  
Mucosal Regeneration ◽  
Postoperative Control ◽  
Inflammation Signs

The healing of oral lesions that are associated with diabetes mellitus is a matter of great concern. Bioactive glass is a highly recommended bioceramic scaffold for bone and soft tissue regeneration. In this study, we aimed to assess the efficacy of a novel formula of bioactive glass nanofibers in enhancing oral mucosal wound regeneration in diabetes mellitus. Bioactive glass nanofibres (BGnf) of composition (1–2) mol% of B2O3, (68–69) mol% of SiO2, and (29–30) mol% of CaO were synthesized via the low-temperature sol-gel technique followed by mixing with polymer solution, then electrospinning of the glass sol to produce nanofibers, which were then subjected to heat treatment. X-Ray Diffraction analysis of the prepared nanofibers confirmed its amorphous nature. Microstructure of BGnf simulated that of the fibrin clot with cross-linked nanofibers having a varying range of diameter (500–900 nm). The in-vitro degradation profile of BGnf confirmed its high dissolution rate, which proved the glass bioactivity. Following fibers preparation and characterization, 12 healthy New Zealand male rabbits were successfully subjected to type I diabetic induction using a single dose of intravenous injection of alloxan monohydrate. Two weeks after diabetes confirmation, the rabbits were randomly divided into two groups (control and experimental groups). Bilateral elliptical oral mucosal defects of 10 × 3.5 mm were created in the maxillary mucobuccal fold of both groups. The defects of the experimental group were grafted with BGnf, while the other group of defects considered as a control group. Clinical, histological, and immune-histochemical assessment of both groups of wounds were performed after one, two and three weeks’ time interval. The results of the clinical evaluation of BGnf treated defects showed complete wound closure with the absence of inflammation signs starting from one week postoperative. Control defects, on the other hand, showed an open wound with suppurative exudate. On histological and immunohistochemical level, the BGnf treated defects revealed increasing in cell activity and vascularization with the absence of inflammation signs starting from one week time interval, while the control defects showed signs of suppurative inflammation at one week time interval with diminished vascularization. The results advocated the suitability of BGnf as bioscaffold to be used in a wet environment as the oral cavity that is full of microorganisms and also for an immune-compromised condition as diabetes mellitus.

scholarly journals Clinical and cytological characteristic of a condition of the oral cavity at pregnant women with various types of a diabetes mellitus

2016 ◽  
Vol 65 (6) ◽  
pp. 45-51 ◽  
Author(s):  
Ludmila Yu Orekhova ◽  
Anna A Aleksandrova ◽  
Ludmila A Aleksandrova ◽  
Ramila S Musaeva ◽  
Gulrukhsor Kh Tolibova ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Oral Cavity ◽  
Pregnant Women ◽  
Inflammatory Diseases ◽  
Type I Diabetes ◽  
Periodontal Diseases ◽  
Diabetes Mellitus Type 1 ◽  
Control Group ◽  
Type I ◽  
Liquid Based Cytology

Introduction. More and more researches dedicated to the communication of diseases of the oral cavity of pregnant women with diabetes. It is proved that the intensity of caries and inflammatory periodontal diseases (gingivitis and periodontitis) increase significantly during pregnancy, while the presence of comorbidities, such as diabetes, increase these indexes.Aim. The aim of the work was to study the dental status of pregnant women with diabetes.Materials and methods. The study compared women with gestational diabetes mellitus, type 1 diabetes, and type 2 diabetes, to a control group of pregnant women without diabetes. In addition to clinical research methods, liquid-based cytology of the contents of the gingival sulcus was performed.Results. The results of clinical and laboratory studies have shown that inflammatory diseases of periodontium and teeth within pregnant women with diabetes are more common than within the pregnant women without this disease. It should be noted that the frequency of occurrence and severity of these diseases in the pregnant women with type I diabetes is higher than in the other groups.Conclusion. Pregnant women with diabetes are at risk for dental disease and require more attention from dentists, endocrinologists and obstetricians. The use of liquid-based cytology method helps in the diagnosis of inflammatory periodontal diseases.

scholarly journals EFFECTS OF PROBIOTICS SUPPLEMENTATION ON SKIN WOUND HEALING IN DIABETIC RATS

2020 ◽  
Vol 33 (1) ◽  
Author(s):  
Letícia Fuganti CAMPOS ◽  
Eliane TAGLIARI ◽  
Thais Andrade Costa CASAGRANDE ◽  
Lúcia de NORONHA ◽  
Antônio Carlos L. CAMPOS ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Type I Collagen ◽  
Chronic Wounds ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Control Group ◽  
Type I ◽  
Collagen Deposition ◽  
Skin Wound Healing

ABSTRACT Background: Chronic wounds in patients with Diabetes Mellitus often become incurable due to prolonged and excessive production of inflammatory cytokines. The use of probiotics modifies the intestinal microbiota and modulates inflammatory reactions. Aim: To evaluate the influence of perioperative supplementation with probiotics in the cutaneous healing process in diabetic rats. Methods: Forty-six rats were divided into four groups (C3, P3, C10, P10) according to the treatment (P=probiotic or C=control, both orally administered) and day of euthanasia, 3rd or 10th postoperative days. All rats were induced to Diabetes Mellitus 72 h before starting the experiment with alloxan. Supplementation was initiated five days before the incision and maintained until euthanasia. Scalpel incision was guided by a 2x2 cm mold and the wounds were left to heal per second-intention. The wounds were digitally measured. Collagen densitometry was done with Picrosirius Red staining. Histological parameters were analyzed by staining by H&E. Results: The contraction of the wound was faster in the P10 group which resulted in a smaller scar area (p=0.011). There was an increase in type I collagen deposition from the 3rd to the 10th postoperative day in the probiotic groups (p=0.016), which did not occur in the control group (p=0.487). The histological analysis showed a better degree of healing in the P10 group (p=0.005), with fewer polymorphonuclear (p<0.001) and more neovessels (p=0.001). Conclusions: Perioperative supplementation of probiotics stimulates skin wound healing in diabetic rats, possibly due to attenuation of the inflammatory response and increased neovascularization and type I collagen deposition.

Glutamate Release by Osteoblasts in the Presence of Ionic Products from Bioactive Glass 60S

2005 ◽  
Vol 284-286 ◽  
pp. 537-540
Author(s):  
Patricia Valério ◽  
C.C.P. Mendes ◽  
Marivalda Pereira ◽  
Alfredo Goes ◽  
M. Fatima Leite
Keyword(s):  
Bioactive Glass ◽  
Calcium Channel ◽  
Intracellular Calcium ◽  
Subcellular Distribution ◽  
Glutamate Release ◽  
Calcium Entry ◽  
The Other ◽  
Inositol Triphosphate ◽  
Type I ◽  
Type Ii

Osteoblasts constitutively release glutamate and this release appears to be regulated by calcium entry. In this work we investigated if the bioactive glass with 60% of silicon (BG60S) could alter glutamate release by osteoblasts. We demonstrated that osteoblasts incubated with medium containing ionic products from the dissolution of BG60S showed lower release of glutamate when compared to control. Since intracellular calcium (Cai 2+) increase is required for glutamate release we investigated the subcellular distribution of the calcium channel inositol triphosphate receptors (InsP3Rs) in the presence of BG60S compared to control. We found that the type-III InsP3R was not expressed in osteoblast, while the type-II InsP3R was expressed mainly in the cytosol. We also found that the expression of type-II InsP3R decreased in BG60S treated osteoblasts compared to control. On the other hand, we found that the type-I InsP3R was expressed mainly in the nucleus and its expression increased in the presence of the biomaterial.

scholarly journals ORAL CARE AND CHOICE OF MEDICINES FOR TREATING GINGIVITIS AND PERIODONTITIS IN CHILDREN WITH TYPE 1 DIABETES MELLITUS

2020 ◽  
Vol 20 (1) ◽  
pp. 199-204
Author(s):  
I.O. Kuz ◽  
O.V. Sheshukova
Keyword(s):  
Diabetes Mellitus ◽  
Inflammatory Diseases ◽  
Type I Diabetes ◽  
Periodontal Diseases ◽  
Oral Care ◽  
Time Interval ◽  
Type I ◽  
Periodontal Tissues ◽  
Prophylactic Measures ◽  
Dental Appointment

The anatomical and physiological characteristics of a growing organism require a specific approach to the prevention and treatment of periodontal diseases. Not enough attention is paid toward improving the oral health of children suffering from diabetes mellitus, as well as therapeutic and prophylactic measures aimed at preserving teeth and preventing exacerbations of the inflammatory process in periodontal tissues. The results of clinical studies indicate a high prevalence of dental diseases in patients with type I diabetes mellitus; there are not enough reliable results of such indicators depending on the age and duration of this endocrine pathology, diabetic complications, hypoglycemic therapy taking into account metabolic control. Diagnosis of periodontal diseases is complicated by the peculiarities of the child’s psychological perception, caused by age-specific characteristics and the influence of common manifestations of inflammatory diseases. Children of primary school age often demonstrate increased motor activity, a hyperergic reaction even to minor negative feelings, the difficulty of adequate behaviour in an unfamiliar environment and a correct assessment of what is happening, being stressed due to the presence of many phobias associated with doctors and medical manipulations, communicative difficulties caused by staying in the spotlight, meeting new people, talking with adults. Teaching children must necessarily be accompanied by educating parents, since within limited time interval of dental appointment a doctor does not have the opportunity to fully monitor and consolidate the knowledge and skills the child has received, and this responsibility falls on parents. In this case, some attention should be paid to issues of skills, motivation as well as on the importance dental care aspects for the development of the dentofacial system, since in the vast majority of cases, parents are not enough aware on these issues. In addition to educating and fostering the motivation, it is necessary to control the level of knowledge, the frequency and quality of tooth brushing. For young children, it is necessary to do complete cleaning up the chewing group of teeth due to the low level of children’s manual skills.

Removal Pathway of Bioactive Glass Resorption Products from the Body

1999 ◽  
Vol 599 ◽  
Author(s):  
W. Lai ◽  
P. Ducheyne ◽  
J. Garino ◽  
C. M. Flaitz
Keyword(s):  
Bioactive Glass ◽  
Excretion Rate ◽  
Atomic Absorption Spectrophotometry ◽  
The Body ◽  
The Other ◽  
Soluble Form ◽  
Control Group ◽  
Flame Atomic Absorption ◽  
Physiological Capacity

AbstractWe traced and quantified the silicon released from bioactive glass (BG) granules in vivo (45S5, 300–355 μm). 1500 mg of BG granules were implanted in the paraspinal muscle of 7 four kg rabbits. Blood samples and 24-hour urine samples were obtained over a 24 week period. Local muscle tissue as well as the following organs were harvested for chemical and histological analyses: brain, heart, kidney, liver, lung, lymph nodes, spleen, and thymus. Flame atomic absorption spectrophotometry was used to measure the concentration of elemental silicon in all the samples after digestion. Tissues and fluids from a sham group of 7 rabbits (underwent surgical procedure but received no implants) were obtained in a similar manner.The urinary silicon of the implanted group was significantly higher than in the control group. From the data, the calculated average excretion rate was approximately 2.4 mg/day, and as such, 100 percent of the implanted silicon was excreted in 19 weeks. No elevated concentrations of silicon were found at the implant site or in the other organs after 24 weeks. Histological appearance of all major organs was normal for all animals in the study.The concentrations of silicon measured in the urine were well below saturation and since no significant increase in silicon was found in the kidney or in the other organs, the increased silicon excretion rate was within the physiological capacity of rabbits. Therefore, it can be concluded that the resorbed silica gel is harmlessly excreted in soluble form through the urine.

scholarly journals Terminal ileum submucous plexus: Study of the VIP-ergic neurons of diabetic rats treated with ascorbic acid

2002 ◽  
Vol 60 (1) ◽  
pp. 32-37 ◽  
Author(s):  
Jacqueline Nelisis Zanoni ◽  
Luzmarina Hernandes ◽  
Roberto Barbosa Bazotte ◽  
Marcílio Hubner de Miranda Neto
Keyword(s):  
Diabetes Mellitus ◽  
Ascorbic Acid ◽  
Glycated Hemoglobin ◽  
Experimental Diabetes ◽  
Diabetic Rats ◽  
The Other ◽  
Control Group ◽  
Small Reduction ◽  
Experimental Diabetes Mellitus ◽  
Submucous Plexus

The aim of this study was to evaluate the effect of the ascorbic acid (AA) supplementation on the neurons that produce the vasoactive intestinal peptide (VIP) in the submucous plexus of the ileum of rat, four months after the induction of experimental diabetes mellitus with streptozotocin. Three groups of rats were used: C - control, D - diabetic, DA - diabetic receiving AA. We have measured the immunoreactivity and area of 80 cellular bodies of VIP-ergic neurons from each studied group. In the diabetic animals, we have observed hyperphagia, polydipsia, and an increase of glycemia and glycated hemoglobin. The VIP-ergic neurons have presented an increase of their immunoreactivity and the highest profiles when compared to the other groups. In the diabetic animals supplemented with AA it has been observed a small reduction in the glycemia and the water and food intake. We have also noticed smaller immunoreactivity in their VIP-ergic neurons, similar to what we have observed in the control group animals (group C).

Evaluation of Bone Mineral Density in Children with Type I Diabetes Mellitus and Relationship to Serum Levels of Osteopontin

Drug Research ◽  
2017 ◽  
Vol 67 (09) ◽  
pp. 527-533 ◽  
Author(s):  
Forough Saki ◽  
Abdolkarim Sheikhi ◽  
Gholam Omrani ◽  
Hamid Karimi ◽  
Mohammad Dabbaghmanesh ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Bone Mineral Density ◽  
Bone Mineral ◽  
Negative Correlation ◽  
Healthy Subjects ◽  
Type I Diabetes ◽  
Serum Levels ◽  
Control Group ◽  
Type I ◽  
Mineral Density

Abstract Type I diabetes mellitus (T1DM) patients are at risk of osteoporosis and fracture due to the osteoblast and osteoclast malfunction. Osteopontin (OPN) as the major non-collagenous bone matrix protein is produced by osteoblasts and osteoclasts and involve in bone resorption, formation and remodeling. To evaluate the serum levels of OPN, bone mineral density (BMD) and correlation in patients with T1DM this study was designed. In this case-control study, 87 children with T1DM and 87 age/sex-matched healthy subjects were enrolled. Blood samples were tested for OPN levels by ELISA. Dual energy X-ray absorptiometry was used to measure BMD. The mean levels of BMD in patients was significantly lower than control group (p<0.0001). There was no significant difference between patients and healthy subjects regarding the levels of OPN. However, in patients with high levels of OPN (mean+1.5 standard deviation) the BMD was significantly lower than other patients (p<0.0001). Totally there was a negative correlation between serum levels of OPN and BMD in patients with T1DM (p<0.016). These results indicated that the BMD in T1DM is less than healthy children and elevated level of OPN in patients could be associated with low BMD. A linear negative correlation between serum OPN and total BMD of T1DM patients compared to control group was noticed in this study indicating that the amount of serum OPN could be effective on BMD and a good predicting factor for osteoporosis in patients.

Evaluation of α-klotho level in insulin dependent diabetes mellitus (IDDM) children

2020 ◽  
Vol 33 (6) ◽  
pp. 761-765
Author(s):  
Fariba Tarhani ◽  
Ghobad Heidari ◽  
Alireza Nezami
Keyword(s):  
Diabetes Mellitus ◽  
Type I Diabetes ◽  
Sandwich Elisa ◽  
Serum Levels ◽  
Dependent Diabetes Mellitus ◽  
Control Group ◽  
Case Group ◽  
Diabetic Patients ◽  
Type I ◽  
And Control

AbstractObjectivesReduced levels of α-Klotho is associated with the pathogenesis of various diseases including diabetes. In type I diabetes, decrease in Klotho leads to apoptosis of β-cells of pancreases. The aim of this study was to evaluate the levels of α-Klotho in type I diabetic pediatric patients.MethodsIn this cross-sectional single centered study, 46 patients presenting type I diabetes mellitus (case group) and 78 control group under the age of 12, referred to our clinic were included in our study. Serum levels of soluble Klotho were measured by sandwich ELISA in case and control groups. Statistical analysis was conducted for the data recorded via questionnaire.ResultsMean age of the patients in the case and control group was 7.65 ± 3.09 and 7 ± 2.37, respectively. Type I diabetes patients had a significant reduction in the levels of serum Klotho, as compared to controls (p<0.001). However, gender and age-based comparison between patient and control group was not significant.ConclusionsThis study reports a significant decrease in the serum levels of α-Klotho in type 1 diabetic patients. Low levels of Klotho can be associated with diabetic nephropathy and other comorbidities in these patients.

scholarly journals Correction of dyslipidemia with combined use of beta-sitosterol and polyprenyl phosphates in dogs with type 1 diabetes mellitus

2019 ◽  
Vol 2019 (5) ◽  
pp. 12-15
Author(s):  
Вячеслав Анников ◽  
Vyacheslav Annikov ◽  
Александр Наровлянский ◽  
Aleksandr Narovlyanskiy ◽  
Александр Санин ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Type I Diabetes ◽  
Control Group ◽  
Type I ◽  
Upper Limit ◽  
Combined Use ◽  
Polyprenyl Phosphates

This study considers the efficiency of use of a combined drug based on beta-sitosterol and polyprenyl phosphates in dogs with type I diabetes mellitus complicated by hyperlipidemia. It was shown that after 1 month of the therapy, there was a significant decrease of the level of cholesterol, triglycerides and glucose vs. control animals. After 2 months of the therapy, in the control group the level of cholesterol and triglycerides was at the upper limit of the norm, which can lead to an exacerbation of the disease in future.

scholarly journals Triglycerides Predict Plasma Fibronectin in Children with Type I Diabetes Mellitus Rather than Diabetes

2002 ◽  
Vol 45 (1) ◽  
pp. 33-37 ◽  
Author(s):  
Ayşe Binnur Erbağcı ◽  
Mehmet Tarakçıoğlu ◽  
Ahmet Erbağcı ◽  
Mehmet Gözübüyük ◽  
Necat Yılmaz ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type I Diabetes ◽  
Plasma Cholesterol ◽  
Endothelial Activation ◽  
Type I Diabetes Mellitus ◽  
Control Group ◽  
Type I ◽  
Plasma Fibronectin ◽  
Diabetic Children ◽  
Adhesive Proteins

Fibronectins are adhesive proteins considered as markers of endothelial activation. Plasma fibronectin levels in diabetes mellitus (DM) have been found to be associated with atherosclerotic risk factors. This study was carried out to investigate plasma fibronectin and its relation with serum lipids, apolipoproteins AI, B100 and lp(a) in diabetic children. 35 children (19F/16M) with type I DM and 30 non-diabetic age and gender-matched controls were enrolled. Apolipoprotein and fibronectin concentrations were determined with nephelometric methods. Plasma fibronectin levels of the children with type I DM and the control group are not statistically different. HbA1c and triglycerides concentration are found to be significant predictors of plasma fibronectin in diabetic children, while effect of plasma cholesterol, apolipoprotein AI, B100 and lp(a) are insignificant. Diabetic children with triglycerides 1.13 mmol/l have elevated plasma fibronectin (median, 25th–75th percentiles; 29.6, 8.3–40.8 mg/dL) compared to the diabetic ≥19.9, 8.6–30.7 mg/dL, p<0,05) and non-diabetic children (16.6, 12.7–32.4 mg/dL, p<0.01) with triglycerides<1.13mmol/L. On the other hand plasma fibronectin concentrations of diabetic and non-diabetic children with high triglycerides are not significantly different. In conclusion our data does not support the concept that plasma fibronectin is elevated in type I diabetes mellitus at least in children, but high plasma triglycerides secondary to diabetes or not is associated with higher FNp concentrations which may have implications on atherogenesis. Plasma cholesterol, apolipoproteins AI, B100 and lp (a) are not significant determinants of FNp in type I diabetic children.

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