Effect of Different Root Canal Irrigant Solutions on the Release of Dentin-Growth Factors: A Systematic Review and Meta-Analysis

Irrigant solutions are used to promote dentin-growth factors (GF) release for regenerative endodontics. This review aimed to evaluate the reports comparing the release of GFs using different root canal irrigant solutions. Eligible studies compared the in vitro GF release in human teeth after the use of at least two distinct solutions. A search was conducted on Pubmed, Scopus, Web of Science, and Lilacs on 11 August 2021. Risk of bias was assessed using SciRAP. Study characteristics and quantitative data were extracted, and meta-analyses were performed for the mean difference (95% confidence interval) of the release of transforming growth factors Beta 1 (TGF-β1) by EDTA compared to other irrigants. Of sixteen eligible studies, eight were included in quantitative analysis. ELISA assays showed higher TGF-β1 release from 10% EDTA compared to 10% citric acid (p < 0.00001). Immunogold assays showed higher levels of TGF-β1 for 17% EDTA (p < 0.00001) compared to 10% citric acid. GRADE identified a low to very low certainty of evidence. These results point to an increased release of TGF-β1 in dentin treated with EDTA. The high heterogeneity and very low certainty of the evidence demand further studies before EDTA indication as a better irrigant for regenerative endodontics. Registration: CRD42020160871 (PROSPERO).

Download Full-text

Comparative in vitro SEM study of a novel root canal irrigant-MTAD and conventional root biomodifiers on periodontally involved human teeth

Journal of Indian Society of Periodontology ◽

10.4103/0972-124x.154192 ◽

2015 ◽

Vol 19 (3) ◽

pp. 268 ◽

Cited By ~ 2

Author(s):

Charu Tandon ◽

Vivek Govila ◽

VandanaAditya Pant ◽

Ajita Meenawat

Keyword(s):

Root Canal ◽

Human Teeth ◽

Sem Study ◽

Root Canal Irrigant

Download Full-text

Comparative Evaluation of Cytotoxicity of a Novel Neem-Lemongrass Formulation and 3% Sodium Hypochlorite as a Root Canal Irrigant - An In Vitro Study

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2020.sp1.429 ◽

2020 ◽

Vol 12 (sp1) ◽

Keyword(s):

Sodium Hypochlorite ◽

Root Canal ◽

Comparative Evaluation ◽

In Vitro Study ◽

Vitro Study ◽

Root Canal Irrigant

Download Full-text

Contribution of XP‐Endo files to the root canal filling removal: A systematic review and meta‐analysis of in vitro studies

Australian Endodontic Journal ◽

10.1111/aej.12503 ◽

2021 ◽

Author(s):

Emel Uzunoglu‐Özyürek ◽

Selen Küçükkaya Eren ◽

Sevilay Karahan

Keyword(s):

Systematic Review ◽

Root Canal ◽

Meta Analysis ◽

In Vitro Studies ◽

Root Canal Filling

Download Full-text

Effectiveness of a silicon-based root canal sealer for filling of simulated lateral canals

Brazilian Dental Journal ◽

10.1590/s0103-64402007000100005 ◽

2007 ◽

Vol 18 (1) ◽

pp. 20-23 ◽

Cited By ~ 2

Author(s):

João Vicente Baroni Barbizam ◽

Matheus Souza ◽

Doglas Cecchin ◽

Jakob Dabbel

Keyword(s):

Root Canal ◽

In Vitro Study ◽

Root Canal Sealer ◽

Human Teeth ◽

Significance Level ◽

Working Length ◽

Group 3 ◽

Group 2 ◽

Silicon Based

The purpose of this in vitro study was to evaluate the ability of a silicon-based root canal sealer, compared to zinc oxide and eugenol and an epoxy resin-based sealers, for filling of simulated lateral canals. Thirty extracted single-rooted human teeth were selected, conventional access was made and the working length was established 1 mm from the apical foramen. Three simulated lateral canals, one in each root third (coronal, middle and apical) were prepared in both the mesial and distal surfaces of each tooth using a size 15 reamer adapted to a low-speed handpiece. Each root canal was instrumented using ProTaper rotary files up to file F3 at the working length, and then irrigated with 2.5% NaOCl followed by EDTA. The teeth were assigned to 3 groups (n=10), according to the root canal sealer: Roeko Seal (Group 1), Sealer 26 (group 2) and Grossman's sealer (Group 3). Gutta-percha cold lateral condensation technique was performed in all groups. Postoperative radiographs were taken and the images were projected for evaluation of the quality of lateral canal filling. Data were submitted to statistical analysis by Kruskal Wallis test at 5% significance level. The results showed that Grossman's sealer filled a larger number of lateral canals than Roeko Seal (p<0.05) and Sealer 26 (p<0.01). It may be concluded that Roeko Seal silicone-based root canal sealer was not as effective as the Grossman's sealer for filling of simulated lateral canals. The lateral canals localized in the apical third of the root were more difficult to be filled.

Download Full-text

Formulation and characterization of chlorhexidine HCl nanoemulsion as a promising antibacterial root canal irrigant: in-vitro and ex-vivo studies

International Journal of Nanomedicine ◽

10.2147/ijn.s204550 ◽

2019 ◽

Vol Volume 14 ◽

pp. 4697-4708 ◽

Cited By ~ 1

Author(s):

Rehab Abdelmonem ◽

Mona K. Younis ◽

Doaa H Hassan ◽

Mohamed Abd El-Gawad El-Sayed Ahmed ◽

Ehab Hassanien ◽

...

Keyword(s):

Root Canal ◽

Ex Vivo ◽

Root Canal Irrigant

Download Full-text

Comparison of Release Profiles of Various Growth Factors from Biodegradable Carriers

MRS Proceedings ◽

10.1557/proc-530-13 ◽

1998 ◽

Vol 530 ◽

Cited By ~ 1

Author(s):

Y. Tabata ◽

M. Yamamoto ◽

Y. Ikada

Keyword(s):

Growth Factors ◽

Growth Factor ◽

Transforming Growth Factor ◽

Transforming Growth Factor Β1 ◽

The Body ◽

Bone Morphogenetic Protein 2 ◽

Biodegradable Hydrogel ◽

Tgf Β1

AbstractA biodegradable hydrogel was prepared by glutaraldehyde crosslinking of acidic gelatin with an isoelectric point (IEP) of 5.0 as a carrier to release basic growth factors on the basis of polyion complexation. Basic fibroblast growth factor (bFGF), transforming growth factor β1 (TGF-β1), and bone morphogenetic protein-2 (BMP-2) were sorbed from their aqueous solution into the dried gelatin hydrogels to prepare respective growth factor-incorporating hydrogels. Under an in vitro non-degradation condition, approximately 20 % of incorporated bFGF and TGF-β1 was released from the hydrogels within initial 40 min, followed by no further release, whereas a large initial release of BMP-2 was observed. After subcutaneous implantation of the gelatin hydrogels incorporating 125I-labeled growth factor in the mouse back, the remaining radioactivity was measured to estimate the in vivo release profile of growth factors. Incorporation into gelatin hydrogels enabled bFGF and TGF-β1 to retain in the body for about 15 days and the retention period well correlated with that of the gelatin hydrogel. Taken together, it is likely that the growth factors ionically complexed with acidic gelatin were released in vivo as a result of hydrogel biodegradation. On the contrary, basic BMP-2 did not ionically interact with acidic gelatin, resulting in no sustained released by the present biodegradable carrier system.

Download Full-text

What Are the Chances of Resilon to Dominate the Market Filling Materials for Endodontics?

Metals ◽

10.3390/met11111744 ◽

2021 ◽

Vol 11 (11) ◽

pp. 1744

Author(s):

Joanna Dobrzańska ◽

Lech B. Dobrzański ◽

Leszek A. Dobrzański ◽

Anna D. Dobrzańska-Danikiewicz ◽

Klaudiusz Gołombek

Keyword(s):

Root Canal ◽

Swot Analysis ◽

Experimental Studies ◽

Filling Material ◽

Human Teeth ◽

Research Results ◽

Filling Materials ◽

Common Trade ◽

Selection Of

This paper is a literature review with additional virtual analyses of the authors’ own experimental research results. Knowledge from various areas was synergistically combined, appropriately for concurrent engineering, presenting several possible methodological approaches used in research, optimizing the selection of engineering materials and the conditions of their application with particular application in endodontics. Particular attention was paid to the theoretical aspects of filling material strengths, weaknesses, opportunities, and threats SWOT analysis. Attention was paid to the original concepts of Sustainable Dentistry Development in conjunction with Dentistry 4.0, which includes endodontics as an important element. The dentists’ actions, among others, in conservative dentistry, along with endodontics, requires close cooperation with engineers and the enginering sciences. Methods of root canal preparation were described, together with selected tools, including those made of nitinol. Principles concerning the process of cleaning and shaping the pulp complex are presented. The importance of obturation methods, including the Thermo-Hydraulic-Condensation THC technique, and the selection of filling materials with the necessary sealants for the success of endodontic treatment are discussed. The experimental studies were carried out in vitro on human teeth removed for medical reasons, except for caries, for which two groups of 16 teeth were separated. After the root canal was prepared, it was filled with studs and pellets of a filling material based on polyester materials, which has gained the common trade name of resilon or, less frequently, RealSeal (SybronEndo) with an epiphany sealant. The teeth for the first group were obturated by cold lateral condensation. In the second case the obturation was performed using the Thermo-Hydraulic-Condensation technique using System B and Obtura III. The experimental leakage testing was done using a scanning electron microscope SEM and a light stereoscopic microscope LSM, as typical research tools used in materialography. The research results, in a confrontation with the data taken from the literature studies, do not indicate the domination of resilon in endodontics.

Download Full-text