scholarly journals The Correlation between Extracellular Heat Shock Protein 70 and Lipid Metabolism in a Ruminant Model

Metabolites ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 19
Author(s):  
Miloš Ž. Petrović ◽  
Marko Cincović ◽  
Jože Starič ◽  
Radojica Djoković ◽  
Branislava Belić ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Energy Balance ◽  
Heat Shock ◽  
Heat Shock Protein 70 ◽  
Negative Energy ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Cholesterol Ratio ◽  
Tnf Α ◽  
Lipid Metabolites

Metabolic stress in early lactation cows is characterized by lipolysis, ketogenesis, insulin resistance and inflammation because of negative energy balance and increased use of lipids for energy needs. In this study the relationship between lipid metabolite, lipid-based insulin resistance, and hepatocyte functionality indexes and tumor necrosis factor alpha (TNF-α) with extracellular heat shock protein 70 (eHsp70) was investigated. The experiment included 50 cows and all parameters were measured in blood serum. In cows with a more pronounced negative energy balance, the following was determined: a higher concentration of eHsp70, TNF-α, non-esterified fatty acid (NEFA), beta-hydroxybutyrate (BHB), NEFA to insulin and NEFA to cholesterol ratio and lower concentration of cholesterol, very low-density lipoproteins (VLDL), low density lipoproteins (LDL) and liver functionality index (LFI). The eHsp70 correlated negatively with the values of cholesterol, VLDL, LDL, and triglycerides, while correlated positively with the level of NEFA and BHB. A higher concentration of eHsp70 suggests the development of fatty liver (due to a higher NEFA to cholesterol ratio and lower LFI) and insulin resistance (due to a lower revised quantitative insulin sensitivity check index RQUICKI-BHB and higher NEFA to insulin ratio). The eHsp70 correlated positively with TNF-α. Both TNF-α and eHsp70 correlated similarly to lipid metabolites. In cows with high eHsp70 and TNF-α values we found higher concentrations of NEFA, BHB, NEFA to insulin and NEFA to cholesterol ratio and a lower concentration of triglycerides and VLDL cholesterol compared to cows that had only high TNF-α values. Based on the positive correlation between eHsp70 and TNF-α, their similar relations, and the additional effect of eHsp70 (high TNF-α + eHsp70 values) on lipid metabolites we conclude that eHsp70 has pro-inflammatory effects implicating lipolysis, fatty liver, and fat tissue insulin resistance.

Favorable effects of Globularia alypum on cardiometabolic markers in high fructose-fed rats

2020 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Yasmina Bahlil ◽  
Djamil Krouf ◽  
Zoheir Mellouk ◽  
Nawal Taleb-Dida ◽  
Akila Guenzet
Keyword(s):  
Insulin Resistance ◽  
Lipid Peroxidation ◽  
Low Density Lipoproteins ◽  
Standard Diet ◽  
Low Density ◽  
Content Type ◽  
High Fructose Diet ◽  
Globularia Alypum ◽  
High Fructose ◽  
Antioxidant Defense Systems

Purpose This study aims to examine whether Globularia alypum (Ga) lyophilized aqueous leaves extract treatment improves cardiometabolic syndromes such as hyperglycemia, lipid profiles and oxidative damage resulting from a high-fructose diet induced in hypertriglyceridemic rats. Design/methodology/approach A total of 24 male Wistar rats weighing 80 ± 5 g were first randomly divided into 2 groups. A total of 12 control rats (C) were fed a standard-diet (St-D) and 12 high fructose (HF) rats were fed a high-fructose diet (HF-D) containing St-D in which cornstarch was substituted by fructose (61.4%). After 15 weeks of feeding, body weight (BW) was about 320 ± 20 g and hypertriglyceridemia was noted in HF vs C group (2.69 ± 0.49 mmol/L) vs (1.25 ± 0.33 mmol/L). Each group of rats was then divided into two equal groups (n = 6) and fed during four weeks either a St-D or HF-D, treated or not with 1% of Ga extract (C-Ga) and (HF-Ga). After 28 days, fasting rats were anesthetized and blood and tissues were removed to measure biochemical parameters. Findings The results showed no significant differences in BW and insulinemia between all groups. Ga extract supplementation reduced glycemia (−36%), glycosylated hemoglobin (−37%), Homeostasis Model of Assessment-Insulin Resistance index (−34%) and triacylglycerol’s contents in plasma (−33%), very low density lipoproteins–low density lipoproteins (VLDL-LDL) (−48%), liver (−52%) and aorta (−39%); total cholesterol concentrations in aorta was 3.7-fold lower in HF-Ga vs HF group. Ga treatment reduced lipid peroxidation in plasma, VLDL-LDL, red blood cells (RBC), liver, muscle and kidney by improving superoxide dismutase (SOD) activity and glutathione peroxidase (GPx) in RBC and catalase (CAT) activity in kidney (p < 0.05). Moreover, Ga ameliorates glutathione (GSH) production in RBC (+41%) and kidney tissues (+35%). Originality/value Ga extract ameliorated cardiometabolic syndrome by its hypotriglyceridemic effect and prevented development of insulin resistance. It reduces lipid peroxidation by enhancing non-enzymatic (GSH) and enzymatic (SOD, GPx and CAT) antioxidant defense systems in high-fructose hypertriglyceridemic rats. Therefore, supplementation of Ga leaves extract as an adjuvant could be used for the treatment of hypertriglyceridemia and the prevention and/or the management of cardio-metabolic adverse effects.

scholarly journals Insulin resistance in obesity: body-weight or energy balance?

2001 ◽  
Vol 171 (2) ◽  
pp. 293-298 ◽  
Author(s):  
AR Assali ◽  
A Ganor ◽  
Y Beigel ◽  
Z Shafer ◽  
T Hershcovici ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Energy Balance ◽  
Glucose Tolerance Test ◽  
Serum Levels ◽  
Tolerance Test ◽  
Negative Energy ◽  
Negative Energy Balance ◽  
Second Phase ◽  
Third Phase ◽  
Obese Subjects

Weight reduction is recommended for the treatment of subjects with insulin resistance (IR) syndrome; however, the relative importance of the decrease in body fat or the negative energy balance achieved during a hypo-energetic diet in the improvement of this metabolic syndrome is still debated. Therefore, we undertook to study their relative impact on amelioration of the metabolic abnormalities associated with IR in obese subjects. Twelve obese subjects (six males and six females, mean+/-s.d. body mass index 36.1+/-4.7 kg/m(2)) aged 38-57 years were investigated. During the first phase they were fed a hypo-energetic diet for 6 weeks (week 0-6). During the second phase, lasting 4 weeks (week 6-10) they consumed an iso-energetic diet. During the third phase (week 10-16) the subjects were put again on a hypo-energetic diet. Insulin sensitivity (SI) was assessed by an insulin-enhanced, frequently sampled i.v. glucose tolerance test with minimal model analysis. All subjects reduced weight during both hypo-energetic periods: 5.49+/-0.75 and 2.32+/-0.37%, means+/-s.e.m., P<0.005, week 0-6 and 10-16 respectively. One-third of this loss was achieved within the first week of each period. SI increased by 353+/-121 and 147+/-38% (P<0.005), means+/-s.e.m., at the end of both hypo-energetic periods (week 6 vs 0 and 16 vs 10 respectively). Two-thirds of this improvement were observed within the first week of each period (week 1 vs 0 and 11 vs 10 respectively). During the iso-energetic weight-maintaining period (week 10 vs 6), SI decreased by 43.5+/-7.9% (P<0.002). Serum levels of leptin and triglyceride followed a similar pattern, but to a lesser extent. It may be concluded that negative energy balance is more effective when compared with maintaining a stable lower weight in achieving an improvement in the metabolic parameters of the IR syndrome.

scholarly journals Muscle Heat Shock Protein 70 Predicts Insulin Resistance With Aging

2014 ◽  
Vol 70 (2) ◽  
pp. 155-162 ◽  
Author(s):  
L. Chichester ◽  
A. T. Wylie ◽  
S. Craft ◽  
K. Kavanagh
Keyword(s):  
Insulin Resistance ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70

scholarly journals Contribution of rs3211938 polymorphism at CD36 to glucose levels, oxidized low-density lipoproteins, insulin resistance, and body mass index in Mexican mestizos with type-2 diabetes from western Mexico

2021 ◽  
Author(s):  
Beatriz Teresita Martín-Márquez ◽  
Flavio Sandoval-Garcia ◽  
Mónica Vazquez-Del Mercado ◽  
Erika-Aurora Martínez-García ◽  
Fernanda-Isadora Corona-Meraz ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Type 2 Diabetes ◽  
Body Mass Index ◽  
Body Mass ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Glucose Levels ◽  
Western Mexico ◽  
Mexican Mestizos

scholarly journals Plasma metabolite changes in anestrous dairy cows with negative energy balance identified using 1H NMR technology

2021 ◽  
Vol 73 (4) ◽  
pp. 929-937
Author(s):  
F. Zhang ◽  
Z. Wang ◽  
C. Zhao ◽  
Y. Bai ◽  
D. Wang ◽  
...  
Keyword(s):  
Energy Balance ◽  
Dairy Cows ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Dairy Farm ◽  
Negative Energy ◽  
Negative Energy Balance ◽  
Plasma Metabolites ◽  
Low Density ◽  
Hormone Synthesis

ABSTRACT The objective of the present study was to investigate the different plasma metabolites between anestrus and estrus postpartum dairy cows and to provide a theoretical basis for prevention of anestrus in dairy farm cows. In the experiment, one hundred and sixty-seven Holstein dairy cows were selected with similar age and parity. According to the concentration of β-hydroxybutyric acid, non-esterified fatty acids and glucose in plasma during 14 to 21 days in milk, all dairy cows were determined as having a status of energy balance. According to the results of clinical symptom, rectal and B ultrasound examination at 60 to 90 days postpartum, these cows were divided into twenty estrus and twenty-four anestrus group, other dairy cows were removed. 1H nuclear magnetic resonance technology was utilized to detect the plasma metabolites changes and screen different plasma metabolites between anestrus and estrus cows. Ten different metabolites including alanine, glutamic acid, asparagine, creatine, choline, phosphocholine, glycerophosphocholine, low-density lipoprotein, and very-low-density lipoprotein were significantly decreased in anestrous cows compared with estrous cows. Metabolic pathway analyses indicated that differential metabolites were primarily involved in amino acid and glycerophospholipid metabolism. These metabolites and their enrichment pathways indicate that reduced steroid hormone synthesis precursors result in lower levels of estradiol and progesterone and cause anestrus in negative energy balance. These data provide a better understanding of the changes that may affect estrus of postpartum dairy cows at NEB status and lay the ground for further research.

scholarly journals Tumor Necrosis Factor-α, Caspase-9 and Heat Shock Protein 70 Expressions with Leukemia Inhibitory Factor in Hydrosalpinx

2020 ◽  
Author(s):  
Hanom Husni Syam ◽  
Tono Djuwantono ◽  
Jusuf S. Effendi ◽  
Ponpon S. Idjradinata ◽  
Tita H. Madjid ◽  
...  
Keyword(s):  
Heat Shock ◽  
Leukemia Inhibitory Factor ◽  
Heat Shock Protein 70 ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Endometrial Receptivity ◽  
Caspase 9 ◽  
Tnf Α ◽  
Factor Α ◽  
Necrosis Factor

Abstract Background The leakage of hydrosalpinx fluid from the tube into the uterine cavity is likely to interfere with normal implantation. Hydrosalpinx fluids contain tumor necrosis factor-α (TNF-α), which induces caspase-9 signal transduction, leading to apoptosis. Endometrial cells inhibit apoptosis by synthesizing heat shock protein 70 (Hsp70). The TNF-α, caspase-9 and Hsp70 factors are closely related to apoptosis. In women with hydrosalpinx, the endometrial receptivity of embryonic implantation processes is low. Endometrial receptivity can be assessed by leukemia inhibitory factor (LIF). TNF-α, caspase-9 and Hsp70 expression plays an important role in endometrial receptivity disorders in women with hydrosalpinx.Materials and Methods These is an analytic observational, cross-sectional and categorical comparative study was conducted in 44 subjects with and without hydrosalpinx. The present study was performed in Dr. Hasan Sadikin Hospital, Grha Bunda-, Limijati-Maternity and Children Hospital (May-June 2017). Immunohistochemistry was performed with a cutoff based on the ROC. The Mann-Whitney analysis was performed on TNF-α, caspase-9, Hsp70 and LIF in both groups, whereas a correlation regression test was performed to observe the correlation among these protein.Results The present study used the histoscore as a tool to evaluate the expression of variable between study groups. The comparison of the histoscore for parameters between hydrosalpinx and non-hydrosalpinx subjects was TNF-α (12 vs 9; p<0,001), caspase-9 (12 vs 8,5; p<0,001), Hsp70 (6 vs 8; p<0,001), and LIF (9 vs 12; p<0,05), respectively.Conclusion The results showed a significant difference in TNF-α, caspase-9, Hsp70 and LIF (p <0.05) between hydrosalpinx and non-hydrosalpinx patients. Caspase-9 and Hsp70 are inter-connected and related to LIF as a marker in the endometrium receptivity by hydrosalpinx patients.

scholarly journals Association of extracellular heat shock protein 70 and insulin resistance in type 2 diabetes; independent of obesity and C-reactive protein

2018 ◽  
Vol 24 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Hamid Alemi ◽  
Pegah Khaloo ◽  
Soghra Rabizadeh ◽  
Mohammad Ali Mansournia ◽  
Hossein Mirmiranpour ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Type 2 Diabetes ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
C Reactive Protein ◽  
Reactive Protein
Sign in / Sign up

Export Citation Format

Share Document