scholarly journals A Passive Microfluidic Device for Chemotaxis Studies

Micromachines ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 551 ◽  
Author(s):  
Maria Laura Coluccio ◽  
Maria Antonia D’Attimo ◽  
Costanza Maria Cristiani ◽  
Patrizio Candeloro ◽  
Elvira Parrotta ◽  
...  
Keyword(s):  
Cell Migration ◽  
Microfluidic Device ◽  
Cell Biology ◽  
Scientific Literature ◽  
Microfluidic Devices ◽  
Microfluidic System ◽  
Micro Milling ◽  
Flow Rates ◽  
Free Environment ◽  
Liquid Flows

This work presents a disposable passive microfluidic system, allowing chemotaxis studies, through the generation of a concentration gradient. The device can handle liquid flows without an external supply of pressure or electric gradients, but simply using gravity force. It is able to ensure flow rates of 10 µL/h decreasing linearly with 2.5% in 24 h. The device is made of poly(methylmethacrylate) (PMMA), a biocompatible material, and it is fabricated by micro-milling and solvent assisted bonding. It is assembled into a mini incubator, designed properly for cell biology studies in passive microfluidic devices, which provides control of temperature and humidity levels, a contamination-free environment for cells with air and 5% of CO2. Furthermore, the mini incubator can be mounted on standard inverted optical microscopes. By using our microfluidic device integrated into the mini incubator, we are able to evaluate and follow in real-time the migration of any cell line to a chemotactic agent. The device is validated by showing cell migration at a rate of 0.36 µm/min, comparable with the rates present in scientific literature.

A Multifunctional Microfluidic Device Based on Bifurcation Geometry

2010 ◽  
Author(s):  
Ahmed Fadl ◽  
Stefanie Demming ◽  
Zongqin Zhang ◽  
Bjo¨rn Hoxhold ◽  
Stephanus Bu¨ttgenbach ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Microfluidic Devices ◽  
Optical Lithography ◽  
Working Fluid ◽  
The Novel ◽  
Flow Rates ◽  
Single Function ◽  
Fluidic Device ◽  
Bifurcation Structures ◽  
Novel Design

Developing multifunctional devices are essential to realize more efficient Microsystems. With miniaturization processes taking place in many different applications, the rooms for single function microfluidic devices are limited. In this study, we introduce a multifunctional micro fluidic device based on bifurcation geometry which is capable of performing pumping and mixing at the same time. Optical lithography is used to fabricate the designed microfluidic device. The microfluidic device is tested at low actuator frequencies, and ethanol is employed as a working fluid. The operational principles are based on rectifying the oscillatory flows by using bifurcation structures for flow rectification. The results prove the feasibility of the novel design, and results are presented in terms of flow rates and maximum back pressures.

scholarly journals Low Cost Micro Milling Machine for Prototyping Plastic Microfluidic Devices

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 707 ◽  
Author(s):  
Md Mubarak Hossain ◽  
Tanzilur Rahman
Keyword(s):  
Developing Countries ◽  
Microfluidic Device ◽  
Low Cost ◽  
Microfluidic Devices ◽  
Micro Milling ◽  
Biological Research ◽  
Milling Machine ◽  
Plastic Materials ◽  
Milling Machines

Micro-milling is one of the commonly used methods of fabrication of microfluidic devices necessary for cell biological research and application. Commercial micro-milling machines are expensive, and researchers in developing countries can’t afford them. Here, we report the design and the development of a low-cost (<130 USD) micro milling machine and asses the prototyping capabilities of microfeatures in plastic materials. We demonstrate that the developed machine can be used in fabricating the plastic based microfluidic device.

scholarly journals Modular pumps as programmable hydraulic batteries for microfluidic devices

TECHNOLOGY ◽  
2017 ◽  
Vol 05 (01) ◽  
pp. 21-30 ◽  
Author(s):  
Brian M. Cummins ◽  
Rukesh Chinthapatla ◽  
Balaji Lenin ◽  
Frances S. Ligler ◽  
Glenn M. Walker
Keyword(s):  
Microfluidic Device ◽  
Microfluidic Channel ◽  
Microfluidic Devices ◽  
Complex Flow ◽  
Oscillating Flows ◽  
Flow Rates ◽  
Simple Fluid ◽  
Flow Profiles ◽  
Over Time

Simple fluid pumps have been developed to improve microfluidic device portability, but they cannot be easily programmed, produce repeatable pumping performance, or generate complex flow profiles — key requirements for increasing the functionality of portable microdevices. We present a detachable, paper-based, “hydraulic battery” that can be connected to the outlet of a microfluidic channel to pump fluid at varying flow rates over time, including step changes, ramping flows, and oscillating flows.

scholarly journals Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Device for a Biosensor †

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1178 ◽  
Author(s):  
Jorge Prada ◽  
Christina Cordes ◽  
Carsten Harms ◽  
Walter Lang
Keyword(s):  
Microfluidic Device ◽  
Heating Temperature ◽  
Low Cost ◽  
Reaction Chamber ◽  
Microfluidic System ◽  
Heating Process ◽  
Design And Manufacturing ◽  
On Chip ◽  
Working Parameters ◽  
Auto Fluorescence

This contribution outlines the design and manufacturing of a microfluidic device implemented as a biosensor for retrieval and detection of bacteria RNA. The device is fully made of Cyclo-Olefin Copolymer (COC), which features low auto-fluorescence, biocompatibility and manufacturability by hot-embossing. The RNA retrieval was carried on after bacteria heat-lysis by an on-chip micro-heater, whose function was characterized at different working parameters. Carbon resistive temperature sensors were tested, characterized and printed on the biochip sealing film to monitor the heating process. Off-chip and on-chip processed RNA were hybridized with capture probes on the reaction chamber surface and identification was achieved by detection of fluorescence tags. The application of the mentioned techniques and materials proved to allow the development of low-cost, disposable albeit multi-functional microfluidic system, performing heating, temperature sensing and chemical reaction processes in the same device. By proving its effectiveness, this device contributes a reference to show the integration potential of fully thermoplastic devices in biosensor systems.

scholarly journals Microfluidic Network Simulations Enable On-Demand Prediction of Control Parameters for Operating Lab-On-A-Chip-Devices

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1320
Author(s):  
Julia Sophie Böke ◽  
Daniel Kraus ◽  
Thomas Henkel
Keyword(s):  
Fluid Management ◽  
Lab On A Chip ◽  
Microfluidic System ◽  
Flow Rates ◽  
Demand Prediction ◽  
Fast Running ◽  
Microfluidic Network ◽  
Fluid Properties ◽  
Network Simulations ◽  
User Friendly

Reliable operation of lab-on-a-chip systems depends on user-friendly, precise, and predictable fluid management tailored to particular sub-tasks of the microfluidic process protocol and their required sample fluids. Pressure-driven flow control, where the sample fluids are delivered to the chip from pressurized feed vessels, simplifies the fluid management even for multiple fluids. The achieved flow rates depend on the pressure settings, fluid properties, and pressure-throughput characteristics of the complete microfluidic system composed of the chip and the interconnecting tubing. The prediction of the required pressure settings for achieving given flow rates simplifies the control tasks and enables opportunities for automation. In our work, we utilize a fast-running, Kirchhoff-based microfluidic network simulation that solves the complete microfluidic system for in-line prediction of the required pressure settings within less than 200 ms. The appropriateness of and benefits from this approach are demonstrated as exemplary for creating multi-component laminar co-flow and the creation of droplets with variable composition. Image-based methods were combined with chemometric approaches for the readout and correlation of the created multi-component flow patterns with the predictions obtained from the solver.

scholarly journals Cyclooxygenase Inhibition Alters Proliferative, Migratory, and Invasive Properties of Human Glioblastoma Cells In Vitro

2021 ◽  
Vol 22 (9) ◽  
pp. 4297
Author(s):  
Matthew Thomas Ferreira ◽  
Juliano Andreoli Miyake ◽  
Renata Nascimento Gomes ◽  
Fábio Feitoza ◽  
Pollyana Bulgarelli Stevannato ◽  
...  
Keyword(s):  
Cell Migration ◽  
Cell Biology ◽  
Gelatin Zymography ◽  
Viable Cell ◽  
Cell Counting ◽  
Gelatinolytic Activity ◽  
Ep4 Receptor ◽  
And Migration ◽  
Proliferation And Migration

Prostaglandin E2 (PGE2) is known to increase glioblastoma (GBM) cell proliferation and migration while cyclooxygenase (COX) inhibition decreases proliferation and migration. The present study investigated the effects of COX inhibitors and PGE2 receptor antagonists on GBM cell biology. Cells were grown with inhibitors and dose response, viable cell counting, flow cytometry, cell migration, gene expression, Western blotting, and gelatin zymography studies were performed. The stimulatory effects of PGE2 and the inhibitory effects of ibuprofen (IBP) were confirmed in GBM cells. The EP2 and EP4 receptors were identified as important mediators of the actions of PGE2 in GBM cells. The concomitant inhibition of EP2 and EP4 caused a significant decrease in cell migration which was not reverted by exogenous PGE2. In T98G cells exogenous PGE2 increased latent MMP2 gelatinolytic activity. The inhibition of COX1 or COX2 caused significant alterations in MMP2 expression and gelatinolytic activity in GBM cells. These findings provide further evidence for the importance of PGE2 signalling through the EP2 and the EP4 receptor in the control of GBM cell biology. They also support the hypothesis that a relationship exists between COX1 and MMP2 in GBM cells which merits further investigation as a novel therapeutic target for drug development.

scholarly journals 3H1010 Controlled cell migration with ultrasound(Cell Biology III:Cytoskeleton & Motility,Oral Presentation)

2012 ◽  
Vol 52 (supplement) ◽  
pp. S70
Author(s):  
Shinya Murakami ◽  
Yo Otsuka ◽  
Manabu Sugimoto ◽  
Toshiyuki Mitsui
Keyword(s):  
Cell Migration ◽  
Oral Presentation ◽  
Cell Biology

Cell Migration in Microfluidic Devices: Invadosomes Formation in Confined Environments

2019 ◽  
pp. 79-103 ◽  
Author(s):  
Pei-Yin Chi ◽  
Pirjo Spuul ◽  
Fan-Gang Tseng ◽  
Elisabeth Genot ◽  
Chia-Fu Chou ◽  
...  
Keyword(s):  
Cell Migration ◽  
Microfluidic Devices

scholarly journals Micropipette-Based Microfluidic Device for Monodisperse Microbubbles Generation

Micromachines ◽  
2018 ◽  
Vol 9 (8) ◽  
pp. 387
Author(s):  
Carlos Toshiyuki Matsumi ◽  
Wilson José da Silva ◽  
Fábio Kurt Schneider ◽  
Joaquim Miguel Maia ◽  
Rigoberto E. M. Morales ◽  
...  
Keyword(s):  
Electric Fields ◽  
Microfluidic Device ◽  
Soft Lithography ◽  
Low Cost ◽  
Characteristic Curve ◽  
Microfluidic Devices ◽  
Average Diameter ◽  
Internal Diameter ◽  
Medical Diagnostic ◽  
Average Size

Microbubbles have various applications including their use as carrier agents for localized delivery of genes and drugs and in medical diagnostic imagery. Various techniques are used for the production of monodisperse microbubbles including the Gyratory, the coaxial electro-hydrodynamic atomization (CEHDA), the sonication methods, and the use of microfluidic devices. Some of these techniques require safety procedures during the application of intense electric fields (e.g., CEHDA) or soft lithography equipment for the production of microfluidic devices. This study presents a hybrid manufacturing process using micropipettes and 3D printing for the construction of a T-Junction microfluidic device resulting in simple and low cost generation of monodisperse microbubbles. In this work, microbubbles with an average size of 16.6 to 57.7 μm and a polydispersity index (PDI) between 0.47% and 1.06% were generated. When the device is used at higher bubble production rate, the average diameter was 42.8 μm with increased PDI of 3.13%. In addition, a second-order polynomial characteristic curve useful to estimate micropipette internal diameter necessary to generate a desired microbubble size is presented and a linear relationship between the ratio of gaseous and liquid phases flows and the ratio of microbubble and micropipette diameters (i.e., Qg/Ql and Db/Dp) was found.

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