scholarly journals Droplet Microfluidics for Food and Nutrition Applications

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 863
Author(s):  
Karin Schroen ◽  
Claire Berton-Carabin ◽  
Denis Renard ◽  
Mélanie Marquis ◽  
Adeline Boire ◽  
...  
Keyword(s):  
Applied Sciences ◽  
Basic Research ◽  
Droplet Microfluidics ◽  
Time Resolved ◽  
Lab On Chip ◽  
Food And Nutrition ◽  
Research Questions ◽  
On Chip ◽  
Nutritional Sciences

Droplet microfluidics revolutionizes the way experiments and analyses are conducted in many fields of science, based on decades of basic research. Applied sciences are also impacted, opening new perspectives on how we look at complex matter. In particular, food and nutritional sciences still have many research questions unsolved, and conventional laboratory methods are not always suitable to answer them. In this review, we present how microfluidics have been used in these fields to produce and investigate various droplet-based systems, namely simple and double emulsions, microgels, microparticles, and microcapsules with food-grade compositions. We show that droplet microfluidic devices enable unprecedented control over their production and properties, and can be integrated in lab-on-chip platforms for in situ and time-resolved analyses. This approach is illustrated for on-chip measurements of droplet interfacial properties, droplet–droplet coalescence, phase behavior of biopolymer mixtures, and reaction kinetics related to food digestion and nutrient absorption. As a perspective, we present promising developments in the adjacent fields of biochemistry and microbiology, as well as advanced microfluidics–analytical instrument coupling, all of which could be applied to solve research questions at the interface of food and nutritional sciences.

scholarly journals The Rise of the OM-LoC: Opto-Microfluidic Enabled Lab-on-Chip

Micromachines ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1467
Author(s):  
Harry Dawson ◽  
Jinane Elias ◽  
Pascal Etienne ◽  
Sylvie Calas-Etienne
Keyword(s):  
Low Cost ◽  
Optical Components ◽  
New Era ◽  
Lab On Chip ◽  
Highly Sensitive ◽  
Multiple Challenges ◽  
On Chip ◽  
Optical Circuits ◽  
Made In

The integration of optical circuits with microfluidic lab-on-chip (LoC) devices has resulted in a new era of potential in terms of both sample manipulation and detection at the micro-scale. On-chip optical components increase both control and analytical capabilities while reducing reliance on expensive laboratory photonic equipment that has limited microfluidic development. Notably, in-situ LoC devices for bio-chemical applications such as diagnostics and environmental monitoring could provide great value as low-cost, portable and highly sensitive systems. Multiple challenges remain however due to the complexity involved with combining photonics with micro-fabricated systems. Here, we aim to highlight the progress that optical on-chip systems have made in recent years regarding the main LoC applications: (1) sample manipulation and (2) detection. At the same time, we aim to address the constraints that limit industrial scaling of this technology. Through evaluating various fabrication methods, material choices and novel approaches of optic and fluidic integration, we aim to illustrate how optic-enabled LoC approaches are providing new possibilities for both sample analysis and manipulation.

An autonomous lab-on-chip sensor for in situ measurements of seawater total alkalinity

2021 ◽  
Author(s):  
Allison Schaap ◽  
Stathys Papadimitriou ◽  
Socratis Loucaides ◽  
Matthew Mowlem
Keyword(s):  
In Situ Measurements ◽  
Total Alkalinity ◽  
Lab On Chip ◽  
On Chip

scholarly journals Industry Partnership: Lab on Chip Chemical Sensor Technology for Ocean Observing

2021 ◽  
Vol 8 ◽  
Author(s):  
Matt Mowlem ◽  
Alexander Beaton ◽  
Robin Pascal ◽  
Allison Schaap ◽  
Socratis Loucaides ◽  
...  
Keyword(s):  
Chemical Sensor ◽  
Autonomous Underwater Vehicles ◽  
Product Line ◽  
The Arctic ◽  
Sensor Technology ◽  
Lab On Chip ◽  
Chip Technology ◽  
Wide Range ◽  
On Chip

We introduce for the first time a new product line able to make high accuracy measurements of a number of water chemistry parameters in situ: i.e., submerged in the environment including in the deep sea (to 6,000 m). This product is based on the developments of in situ lab on chip technology at the National Oceanography Centre (NOC), and the University of Southampton and is produced under license by Clearwater Sensors Ltd., a start-up and industrial partner in bringing this technology to global availability and further developing its potential. The technology has already been deployed by the NOC, and with their partners worldwide over 200 times including to depths of ∼4,800 m, in turbid estuaries and rivers, and for up to a year in seasonally ice-covered regions of the arctic. The technology is capable of making accurate determinations of chemical and biological parameters that require reagents and which produce an electrical, absorbance, fluorescence, or luminescence signal. As such it is suitable for a wide range of environmental measurements. Whilst further parameters are in development across this partnership, Nitrate, Nitrite, Phosphate, Silicate, Iron, and pH sensors are currently available commercially. Theses sensors use microfluidics and optics combined in an optofluidic chip with electromechanical valves and pumps mounted upon it to mix water samples with reagents and measure the optical response. An overview of the sensors and the underlying components and technologies is given together with examples of deployments and integrations with observing platforms such as gliders, autonomous underwater vehicles and moorings.

scholarly journals Lab-on-chip analyser for the in situ determination of dissolved manganese in seawater

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Felix Geißler ◽  
Eric P. Achterberg ◽  
Alexander D. Beaton ◽  
Mark J. Hopwood ◽  
Mario Esposito ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Ionic Surfactant ◽  
Lab On Chip ◽  
Inductively Coupled ◽  
Long Term Stability ◽  
In Situ Data ◽  
Laboratory Characterization ◽  
Plasma Mass Spectrometry ◽  
On Chip

AbstractA spectrophotometric approach for quantification of dissolved manganese (DMn) with 1-(2-pyridylazo)-2-naphthol (PAN) has been adapted for in situ application in coastal and estuarine waters. The analyser uses a submersible microfluidic lab-on-chip device, with low power (~ 1.5 W) and reagent consumption (63 µL per sample). Laboratory characterization showed an absorption coefficient of 40,838 ± 1127 L⋅mol−1⋅cm−1 and a detection limit of 27 nM, determined for a 34.6 mm long optical detection cell. Laboratory tests showed that long-term stability of the PAN reagent was achieved by addition of 4% v/v of a non-ionic surfactant (Triton-X100). To suppress iron (Fe) interferences with the PAN reagent, the Fe(III) masking agents deferoxamine mesylate (DFO-B) or disodium 4,5-dihydroxy-1,3-benzenedisulfonate (Tiron) were added and their Fe masking efficiencies were investigated. The analyser was tested during a deployment over several weeks in Kiel Fjord (Germany), with successful acquisition of 215 in situ data points. The time series was in good agreement with DMn concentrations determined from discretely collected samples analysed via inductively coupled plasma mass spectrometry (ICP-MS), exhibiting a mean accuracy of 87% over the full deployment duration (with an accuracy of > 99% for certain periods) and clear correlations to key hydrographic parameters.

Highly parallel SPAD detector for time-resolved lab-on-chip

2010 ◽  
Author(s):  
Michele Benetti ◽  
Daniele Iori ◽  
Lucio Pancheri ◽  
Fausto Borghetti ◽  
Laura Pasquardini ◽  
...  
Keyword(s):  
Time Resolved ◽  
Lab On Chip ◽  
On Chip

scholarly journals Spatial transcriptomics using combinatorial fluorescence spectral and lifetime encoding, imaging and analysis

2021 ◽  
Author(s):  
Tam Vu ◽  
Alexander Vallmitjana ◽  
Joshua Gu ◽  
Kieu La ◽  
Qi Xu ◽  
...  
Keyword(s):  
Machine Learning ◽  
Error Correction ◽  
Basic Research ◽  
Spatial Context ◽  
Fluorescence Lifetime Imaging ◽  
Sequencing Data ◽  
Time Dimension ◽  
Time Resolved ◽  
Ffpe Tissues

Multiplexed mRNA profiling in the spatial context provides important new information enabling basic research and clinical applications. Unfortunately, most existing spatial transcriptomics methods are limited due to either low multiplexing or assay complexity. Here, we introduce a new spatialomics technology, termed Multi Omic Single-scan Assay with Integrated Combinatorial Analysis (MOSAICA), that integrates in situ labeling of mRNA markers in cells or tissues with combinatorial fluorescence spectral and lifetime encoded probes, spectral and time-resolved fluorescence imaging, and machine learning-based target decoding. This technology is the first application combining the biophotonic techniques; Spectral and Fluorescence Lifetime Imaging and Microscopy (FLIM), to the field of spatial transcriptomics. By integrating the time dimension with conventional spectrum-based measurements, MOSAICA enables direct, highly-multiplexed, in situ spatial biomarker profiling in a single round of staining and imaging while providing error correction removal of background autofluorescence. We demonstrate mRNA encoding using combinatorial spectral and lifetime labeling and target decoding and quantification using a phasor-based image segmentation and machine learning clustering technique. We then showcase MOSAICA′s multiplexing scalability in detecting 10-plex targets in fixed colorectal cancer cells using combinatorial labeling of only five fluorophores with facile error-correction and removal of autofluorescent moieties. MOSAICA′s analysis is strongly correlated with sequencing data (Pearson′s r = 0.9) and was further benchmarked using RNAscope™ and LGC Stellaris™. We further apply MOSAICA for multiplexed analysis of clinical melanoma Formalin-Fixed Paraffin-Embedded (FFPE) tissues that have a high degree of tissue scattering and intrinsic autofluorescence, demonstrating the robustness of the approach. MOSAICA represents a simple, versatile, and scalable tool for targeted spatial transcriptomics analysis that can find broad utility in constructing human cell atlases, elucidating biological and disease processes in the spatial context, and serving as companion diagnostics for stratified patient care.

Characterization of Micro-Valves for Lab-on-Chip Device

2007 ◽  
Author(s):  
S. Dutta ◽  
D. Banerjee
Keyword(s):  
Water Quality ◽  
Molecular Recognition ◽  
Reaction Chamber ◽  
Nano Particles ◽  
Reaction Products ◽  
Lab On Chip ◽  
External Power Source ◽  
Gold Nano Particles ◽  
On Chip

The objective of this study is to develop a portable hand held diagnostics platform for monitoring pollutants and water quality testing. We are developing a lab-on-chip (LOC) device for in-situ synthesis of gold nano-particles and for using a colorimetric peptide assay for water quality monitoring. The gold nano-particles are synthesized in-situ in our experiments. The gold nano-particles exhibit various optical properties due to their Surface Plasmon Resonance (SPR). These stabilized mono-disperse gold nano-particles are coated with bio-molecular recognition motifs on their surfaces. The stabilization and functionalization with bio-molecular recognition motif provides flexibility for various applications. For example, the gold nano-particles synthesized by this process are tested for their ability to be recognized by a surface coated with anti-Flg antibodies. The LOC consists of micro-wells housing different reagents and samples that feed to a common reaction chamber. The reaction products are delivered to several waste chambers in a pre-defined sequence to enable subsequent reagents/ samples to flow into the reaction chamber. Passive flow actuation is obtained by capillary driven flow (wicking). Dissolvable micro-structures are used as passive micro-valves that actuate at predefined intervals and do not require any external power source for actuation. The microfluidic chip (LOC) and the dissolvable microstructures are fabricated using soft lithography techniques. The passive valves are incorporated into the microfluidics platform by novel micro-fabrication and bonding techniques.

scholarly journals Evaluation of a Ferrozine Based Autonomous in Situ Lab-on-Chip Analyzer for Dissolved Iron Species in Coastal Waters

2017 ◽  
Vol 4 ◽  
Author(s):  
Felix Geißler ◽  
Eric P. Achterberg ◽  
Alexander D. Beaton ◽  
Mark J. Hopwood ◽  
Jennifer S. Clarke ◽  
...  
Keyword(s):  
Coastal Waters ◽  
Iron Species ◽  
Lab On Chip ◽  
Dissolved Iron ◽  
On Chip
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