scholarly journals Control of Fungal Diseases in Mushroom Crops while Dealing with Fungicide Resistance: A Review

2021 ◽  
Vol 9 (3) ◽  
pp. 585
Author(s):  
Francisco J. Gea ◽  
María J. Navarro ◽  
Milagrosa Santos ◽  
Fernando Diánez ◽  
Jaime Carrasco

Mycoparasites cause heavy losses in commercial mushroom farms worldwide. The negative impact of fungal diseases such as dry bubble (Lecanicillium fungicola), cobweb (Cladobotryum spp.), wet bubble (Mycogone perniciosa), and green mold (Trichoderma spp.) constrains yield and harvest quality while reducing the cropping surface or damaging basidiomes. Currently, in order to fight fungal diseases, preventive measurements consist of applying intensive cleaning during cropping and by the end of the crop cycle, together with the application of selective active substances with proved fungicidal action. Notwithstanding the foregoing, the redundant application of the same fungicides has been conducted to the occurrence of resistant strains, hence, reviewing reported evidence of resistance occurrence and introducing unconventional treatments is worthy to pave the way towards the design of integrated disease management (IDM) programs. This work reviews aspects concerning chemical control, reduced sensitivity to fungicides, and additional control methods, including genomic resources for data mining, to cope with mycoparasites in the mushroom industry.

2021 ◽  
Vol 13 (16) ◽  
pp. 8792
Author(s):  
Milorad Vojvodić ◽  
Renata Bažok

Seed treatment as a method of local application of pesticides in precise agriculture reduces the amount of pesticides used per unit area and is considered to be the safest, cheapest and most ecologically acceptable method of protecting seeds and young plants from pests in the early stages of their development. With the introduction of insecticides from the neonicotinoid group in the mid-1990s, the frequency of seed treatment increased. Due to suspected negative effects on pollinators, most of these insecticides are banned in the European Union. The ban has therefore led to a reduction in the number of active substances approved for seed treatment and to an increased re-use of active substances from the group of pyrethroids as well as other organophosphorus insecticides, which pose potentially very serious risks, perhaps even greater than those of the banned neonicotinoids. The objective of this review is to analyze the advantages and disadvantages of seed treatment and the potential role of insecticide seed treatment in reducing the negative impact of pesticides on the environment. The main disadvantage of this method is that it has been widely accepted and has become a prophylactic protective measure applied to almost all fields. This is contrary to the principles of integrated pest management and leads to an increased input of insecticides into the environment, by treating a larger number of hectares with a lower amount of active ingredient, and a negative impact on beneficial entomofauna. In addition, studies show that due to the prophylactic approach, the economic and technical justification of this method is often questionable. Extremely important for a quality implementation are the correct processing and implementation of the treatment procedure as well as the selection of appropriate insecticides, which have proven to be problematic in the case of neonicotinoids. The ban on neonicotinoids and the withdrawal of seed treatments in oilseed rape and sugar beet has led to increased problems with a range of pests affecting these crops at an early stage of growth. The results of the present studies indicate good efficacy of active ingredients belonging to the group of anthranilic diamides, cyantraniliprole and chlorantraniliprole in the treatment of maize, soybean, sugar beet and rice seeds on pests of the above-ground part of the plant, but not on wireworms. Good efficacy in controlling wireworms in maize is shown by an insecticide in the naturalites group, spinosad, but it is currently used to treat seeds of vegetable crops, mainly onions, to control onion flies and flies on other vegetable crops. Seed treatment as a method only fits in with the principles of integrated pest management when treated seeds are sown on land where there is a positive prognosis for pest infestation.


Plant Disease ◽  
1999 ◽  
Vol 83 (1) ◽  
pp. 71-76 ◽  
Author(s):  
D. J. Royse ◽  
K. Boomer ◽  
Y. Du ◽  
M. Handcock ◽  
P. S. Coles ◽  
...  

Statistical analyses were performed on spatial distributions of mushroom green mold foci caused by Trichoderma spp. in 30 standard Pennsylvania doubles (743 m2 production surface) selected at random from over 900 total crops mapped. Mapped production houses were divided into four tiers of six beds each with 16 sections per bed (total = 384 sections per double). Each section contained approximately 2 m2. Green mold foci were mapped according to presence or absence in each section as they became visible during the course of the mushroom production. There was a trend toward higher disease incidence at the ends of the doubles, although this was not consistent from level to level. Spatial analysis revealed that green mold foci were more likely to occur in neighboring sections along the beds rather than above, below, or across from each other. Cultural practices that were associated with movement along the beds, i.e., nutrient supplementation, spawning, bed tamping, surface covering, etc., were considered the most likely factors influencing the incidence of green mold in spawned compost. Airborne contamination was considered a less likely source of inocula contributing to epidemic development. Sanitation practices that reduce spore loads along the beds are expected to provide the greatest degree of green mold control.


Author(s):  
L.L. Zakharova ◽  
◽  
G.A. Zhorov ◽  
V.I. Dorozhkin ◽  
◽  
...  

Negative impact on the environment as a result of economic activity of man increasingly becomes the character of combined pollution of ecotoxicants, including xenobiotics of radiation and chemical nature. In animal husbandry in ecologically unfavorable regions, sorbtion-detoxication technologies based on the complex application of bioprotective substances of different origin are used to reduce the intake and accumulation of xenobiotics, to normalize the physiological state of animals and to obtain safe products. It is necessary to observe certain principles ensuring compatibility of components, a wide range of bioprotective action, efficiency and safety at their use in developing the compositions of such sorption-detoxifying complexes. The article substantiates the criteria that should be followed in creating sorption-detoxifying complexes and presents groups of drugs with different mechanisms of action (sorbents, antioxidants, immunomodulators, adaptogens and other biologically active substances) for inclusion in the formulations of combined compositions.


Author(s):  
Paula Andrea Castillo-Sanmiguel ◽  
Laura Rocío Cortés-Sánchez ◽  
Jovanna Acero-Godoy

<p>Tomato plants (<em>Solanum lycopersicum</em>) are susceptible to the infection by diverse pathogens that cause devastating diseases such as vascular wilt, which causes great losses at the production level. The fungus <em>Fusarium oxysporum</em> f. sp. <em>lycopersici</em> (<em>Fol</em>) is one of the etiologic agents of this disease and its control lies in the use of synthetic chemicals which generate a negative impact in both health and the environment; thus, it is necessary to implement biological control as a healthier and more efficient alternative. The fungus <em>Trichoderma</em> spp. is a favorable option to be employed as a biocontroller against this pathogen thanks to its antagonist mechanisms, determined by metabolic and genetic characteristics. On the one hand, for <em>Fol</em> it is indispensable the activation of signaling routes such as MAPK Fmk1, MAPK Mpk1 y HOG, while <em>Trichoderma</em> spp. uses effectors involved in the interaction with the plant such as proteins, enzymes and secondary metabolites that also strengthen its immune response against infection, determined by both Pathogen Associated Molecular Patterns (PAMP) and effectors. Therefore, this article makes a review about the mentioned characteristics and suggests a greater application of tools and molecular markers for the management of this disease.</p>


2020 ◽  
Vol 161 ◽  
pp. 02006
Author(s):  
E. V. Baybakova ◽  
E.E. Nefed’eva ◽  
A. A. Okolelova ◽  
V. F. Zheltobriukhov ◽  
E. S. Ermoshina

The most popular and affordable means to protect plants from fungal diseases are fungicides. Development of new products is aimed at preparation of the composition of several substances to achieve the effect of mutual enhancement of action. We used the grains of wheat (Triticum sp. variety Julius) and barley (Hordeum sativum variety Gloria), they were significantly infected with phytopathogenic fungi, which allows us to get reliable data on the effectiveness of active substances of fungicides. All wheat grains treated with fungicides were infected less than the control ones. Analysis of the effect of individual fungicides demonstrated that the fungi were more sensitive to fludioxonil, especially at doses of CF 0: 2 (56.7% of infected grains) and CF 0:4 (36.7% of infected grains) 5.0 and 10.0 g/t. After the treatment of cyproconazole, the smallest number of infected grains was obtained when grains were treated at a dose 5.0 g/t (80.0 % of infected grains). The fungistatic effect of cyproconazole (C) and fludioxonil (F) was greatest when ratios of C : F were 2.5:10; 5:10; 10:2.5; 10:5; 10:10 g/t.


Mycobiology ◽  
2020 ◽  
Vol 48 (4) ◽  
pp. 313-320
Author(s):  
Song Hee Lee ◽  
Hwa Jin Jung ◽  
Seung-Beom Hong ◽  
Jong In Choi ◽  
Jae-San Ryu

2000 ◽  
Vol 66 (5) ◽  
pp. 1890-1898 ◽  
Author(s):  
M. R. Hermosa ◽  
I. Grondona ◽  
E. A. Iturriaga ◽  
J. M. Diaz-Minguez ◽  
C. Castro ◽  
...  

ABSTRACT The most common biological control agents (BCAs) of the genusTrichoderma have been reported to be strains ofTrichoderma virens, T. harzianum, and T. viride. Since Trichoderma BCAs use different mechanisms of biocontrol, it is very important to explore the synergistic effects expressed by different genotypes for their practical use in agriculture. Characterization of 16 biocontrol strains, previously identified as “Trichoderma harzianum” Rifai and one biocontrol strain recognized asT. viride, was carried out using several molecular techniques. A certain degree of polymorphism was detected in hybridizations using a probe of mitochondrial DNA. Sequencing of internal transcribed spacers 1 and 2 (ITS1 and ITS2) revealed three different ITS lengths and four different sequence types. Phylogenetic analysis based on ITS1 sequences, including type strains of different species, clustered the 17 biocontrol strains into four groups: T. harzianum-T. inhamatum complex, T. longibrachiatum, T. asperellum, and T. atroviride-T. koningii complex. ITS2 sequences were also useful for locating the biocontrol strains inT. atroviride within the complex T. atroviride-T. koningii. None of the biocontrol strains studied corresponded to biotypes Th2 or Th4 of T. harzianum, which cause mushroom green mold. Correlation between different genotypes and potential biocontrol activity was studied under dual culturing of 17 BCAs in the presence of the phytopathogenic fungi Phoma betae,Rosellinia necatrix, Botrytis cinerea, andFusarium oxysporum f. sp. dianthi in three different media.


Author(s):  
Warlles Domingos Xavier ◽  
João Vitor de Souza Silva ◽  
Claudinei Martins Guimarães ◽  
Jorge Luís Sousa Ferreira ◽  
Thiago Albuquerque Turozi ◽  
...  

At level word fungal diseases that affect soybean crop are one of the main causes of low productivity and annual losses may reach 21% of total production. In this context, the objective of the study was to evaluate the efficiency of copper-based protectors associated with fungicides to control soybean diseases such as: asian soybean rust (Phakopsora pachyrhizi), target spot of soybean (Corynespora cassiicola) and cercospora leaf blight (Cercospora kikuchii) + frogeye leaf spot (Cercospora sojina) + brown spot (Septoria glycines), which together were considered as late-crop cycle diseases, with impact on grain yield, in the region of Aparecida do Rio Negro – TO, Brazil. Treatments were composed of different rates of copper-based pesticides associated with fungicides like Azimut® (first application), Orkestra® (second application), Ativum® (third application) and Horos® (fourth application) in soybean. Diseases were identified and crop damage evaluations on leaves were performed using LI-COR® portable meter 7 days after the fourth application. At physiological maturity, grain yield was evaluated. Combined rates of fungicides + Unizeb Gold® (1.5 kg ha-1), Difere® (0.5 L ha-1), and NHT® Copper Super at a rate higher than 0.109 L ha-1, were effective to control late crop-cycle diseases in soybean. Associated applications of fungicides + 0.219 L/ha of NHT® Copper Super reduced the severity of Asian soybean rust, target spot of soybean and late crop-cycle diseases with a greater increase in grain yield (4.5 Mg ha-1).


2021 ◽  
Vol 15 (02) ◽  
pp. 179-184
Author(s):  
Basem Battah

The rapid emergence of bacterial resistance worldwide is a serious problem, leading to many therapeutic failures and rendering inactive effective antibiotics currently used . This problem has recently been accelerated by conflicts and its related migration. The antibiotic resistance phenomenon is diffused in Syria with a high rate of multi drug resistance cases in gram negative and gram positive organisms during and after the Syrian crisis as a result of misprescribing and overprescribing of antibiotics. The inappropriate use of antibiotic plays an important role in resistance generation. Hence, big efforts are urgently needed by using phenotypic and genetic analysis of bacterial strains against antibiotics to increase characterization and identification of mutant resistant strains and find new strategies to control the spread of antimicrobial resistance infections. This review highlights the antibacterial resistance problem in Syria, showing its negative impact and presenting a sum of efforts that are urgently needed to overcome this problem.


Author(s):  
O.V. Parfenova ◽  
O.Yu. Peksheva ◽  
N.N. Zaitsevа

We studied mutations of HIV resistance to antiretroviral drugs during the period of widely use of antiretroviral therapy and the characteristics of the circulation of virus subtypes in the subjects of the Volga Federal District (VFD) from 2016 to 2018 on the plasma samples of HIV-infected patients. Genotyping of resistance mutations to antiretroviral drugs and statistical analysis were carried out. The most common mutations determining the resistance in the reverse transcriptase gene, the most common and specific mutations were identified. An analysis of the prevalence level of mutations in the virus resistance to different groups of antiretroviral drugs allows us to develop approaches to prevent the further occurrence of resistant strains of HIV and minimize their negative impact on the patient’s body. With the development of the epidemic, there is an increasing diversity in the landscape of HIV-1 subtypes circulating on the territory of the Volga Federal District. Point mutation patterns associated with antiretroviral therapy failure in different subtypes vary.


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