scholarly journals Comparison of Quantification Methods to Estimate Farm-Level Usage of Antimicrobials in Medicated Feed in Dairy Farms from Québec, Canada

2021 ◽  
Vol 9 (9) ◽  
pp. 1834
Author(s):  
Hélène Lardé ◽  
David Francoz ◽  
Jean-Philippe Roy ◽  
Marie Archambault ◽  
Jonathan Massé ◽  
...  
Keyword(s):  
Correlation Coefficients ◽  
Reference Method ◽  
Feed Additives ◽  
Human Medicine ◽  
Alternative Methods ◽  
Concordance Correlation Coefficient ◽  
Dairy Herds ◽  
Concordance Correlation ◽  
Antimicrobial Usage ◽  
One Year

Monitoring antimicrobial usage (AMU) in dairy cattle is becoming common in a growing number of countries, with the ultimate goal to improve practices, reduce the development of antimicrobial resistance, and protect human health. However, antimicrobials delivered as feed additives can be missed by some of the quantification methods usually implemented. Our objective was to compare three methods of quantification of in-feed AMU in Québec dairy herds. We recruited 101 dairy producers for one year in the Québec province. Quantities of antimicrobials were calculated by farm from: (1) feed mills invoices (reference method); (2) veterinary prescriptions; and (3) information collected during an in-person interview of each producer. We standardized AMU rates in kilograms per 100 cow-years and compared the reference method to both alternative methods using concordance correlation coefficients and Bland–Altman plots. Antimicrobial usage was well estimated by veterinary prescriptions (concordance correlation coefficient (CCC) = 0.66) or by the approximation using producer’s data (CCC = 0.73) when compared with actual deliveries by feed mills. Users of medically important antimicrobials for human medicine (less than 10% of the farms) were easily identified using veterinary prescriptions. Given that veterinary prescriptions were mostly electronic (90%), this method could be integrated as part of a monitoring system in Québec.

scholarly journals Comparison of Quantification Methods to Estimate Farm-Level Usage of Antimicrobials Other than in Medicated Feed in Dairy Farms from Québec, Canada

2021 ◽  
Vol 9 (5) ◽  
pp. 1106
Author(s):  
Hélène Lardé ◽  
David Francoz ◽  
Jean-Philippe Roy ◽  
Jonathan Massé ◽  
Marie Archambault ◽  
...  
Keyword(s):  
Correlation Coefficient ◽  
Reference Method ◽  
Dairy Farms ◽  
Concordance Correlation Coefficient ◽  
Concordance Correlation ◽  
Herd Management ◽  
Management Software ◽  
Sales Data ◽  
Antimicrobial Usage ◽  
One Year

The objective of the study was to compare three quantification methods to a “garbage can audit” (reference method, REF) for monitoring antimicrobial usage (AMU) from products other than medicated feed over one year in 101 Québec dairy farms. Data were collected from veterinary invoices (VET method), from the “Amélioration de la Santé Animale au Québec” provincial program (GOV method), and from farm treatment records (FARM method). The AMU rate was reported in a number of Canadian Defined Course Doses for cattle (DCDbovCA) per 100 cow-years. Electronic veterinary sales data were obtained for all farms for VET and GOV methods. For the FARM method, a herd management software was used by 68% of producers whereas farm treatment records were handwritten for the others; records could not be retrieved in 4% of farms. Overall, agreement was almost perfect between REF and VET methods (concordance correlation coefficient (CCC) = 0.83), but moderate between REF and GOV (CCC = 0.44), and between REF and FARM (CCC = 0.51). Only a fair or slight agreement was obtained between any alternative method of quantification and REF for oral and intrauterine routes. The billing software used by most of Québec’s dairy veterinary practitioners seems promising in terms of surveillance and benchmarking of AMU in the province.

scholarly journals AB0798 IS THERE A REPRODUCIBILITY OF THE HISTOGRAM AND GRAY SCALE IN ECOEXTRUCTURAL DAMAGES IN RHEUMATOTOLOGICAL DISEASES?

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1423.2-1424
Author(s):  
J. A. Mendonça ◽  
I. Siste de Almeida Aoki ◽  
C. C. Cavuto ◽  
V. A. Leandro-Merhi ◽  
J. L. B. D. Aquino
Keyword(s):  
Correlation Coefficient ◽  
Intraclass Correlation Coefficient ◽  
Rheumatic Diseases ◽  
Femoral Condyle ◽  
Intraclass Correlation ◽  
Correlation Coefficients ◽  
Concordance Correlation Coefficient ◽  
Image Method ◽  
Concordance Correlation ◽  
Gray Scale

Background:The gray scale (GS) in high resolution ultrasound is already well validated for use in rheumatological diseases, but the color map or the histogram, can be considered a new proposal, to better define and complement the echotextural damages detection1.Objectives:To calculate the lesions area measures reproducibility index in arthropathies, between 3 blind evaluators and correlate these measures using the GS and the histogram.Methods:Observational and retrospective study approved by the ethics committee of the Pontifical Catholic University of Campinas, with the opinion number: 1.526.307. A total of 29 patients have been assessed (31% males and 69% females) on period 2014 to 2019 in Rheumatology service. A MyLab 50 -Esaote equipment was used with frequency transducer that ranged between 6.0 and 18.0 MHz, 10 different area measures were performed from each recorded images previously, by the GS and the histogram. Statistical analysis: Spearman’s correlation coefficients, Lin’s concordance coefficient (CCC) and the intraclass correlation coefficient (ICC) and their respective 95% confidence intervals, with the SPSS software package for Windows v. 17.0 (SPSS Inc., Chicago, IL, USA).Results:Average age 43.5 ± 21.5 years of age; with disease duration that varied between ≤ 1 month (48.3%) and ≥36 months (24.1%); with the following diseases: juvenile idiopathic arthritis (17.24%); osteoarthritis (13.79%); psoriatic arthritis (13.79%); undifferentiated spondyloarthritis (3.44%); gout (20.68%); rheumatoid arthritis (27.58%) and reactive arthritis (3.44%). A total of 840 measures of exudative (27.58%), proliferative (27.58%) and snowstorm appearance (6.89%) synovitis were performed; femoral-condyle cartilage (3.44%); synovial cyst (3.44%); paratendinitis (6.89%); calcification (3.44%); nail enthesitis (3.44%); tenosynovitis (6.89%) and tophi (10.34%) (Figure 1). The concordance correlation coefficient showed values closer to 1; p <0.001, the intraclass correlation coefficients with excellent reproducibility (ICC ≥ 0.75); p <0.001, always in relation to the three evaluators (Table 1) and the Spearman correlation between the GS and the histogram ranged from rs = 0.665 to rs = 1,000; p <0.001.Conclusion:The histogram can be considered an image method to better identify echotextural damages.References:[1]Mendonça J, Provenza J, Guissa V, et al AB1059 2D Histogram Ultrasound and 3D Ultrasound Correlation in Rheumatic Diseases Annals of the Rheumatic Diseases 2015; 74:1253-1254.Table 1.Concordance Correlation Coefficient (CCC) and Intraclass Correlation Coefficient (ICC):EvaluatorsGS - CCC(IC 95%)p-valorHistogram (IC 95%)p-valor1 e 20,998(0,994-0,999)<0,0010,999(0,995-1,000)<0,0011 e 30,998(0,995-0,999)<0,0010,999(0,995-1,000)<0,0012 e 30,992(0,980-0,997)<0,010,996(0,979-0,999)<0,01Standard by US 2DICCp-valorGS0,997(0,992-0,999)<0,001Histogram0,998(0,992-0,999)<0,001Legends: Gray Scale (GS).Figure 1.Patient with gout: A and B: Tophi area measures (star) in right metatarsos and efusion (arrow) by GS (45 mm2) and histogram (39 mm2), respectively.Disclosure of Interests:José Alexandre Mendonça Speakers bureau: Novartis, Janssen, Bristol, UCB, Isabella Siste de Almeida Aoki: None declared, Caique Chagas Cavuto: None declared, Vânia Aparecida Leandro-Merhi: None declared, José Luis Braga de Aquino: None declared

scholarly journals Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ana Elena Rodríguez-Rodríguez ◽  
Sergio Luis-Lima ◽  
Javier Donate-Correa ◽  
Laura Diaz-Martín ◽  
María Rosa Arnau ◽  
...  
Keyword(s):  
Plasma Clearance ◽  
Reference Method ◽  
Dried Blood Spot ◽  
Renal Diseases ◽  
Concordance Correlation Coefficient ◽  
Concordance Correlation ◽  
Total Blood ◽  
Simple Method ◽  
Iohexol Plasma Clearance ◽  
Blood Spot

AbstractThere is no simple method to measure glomerular filtration rate (GFR) in mice, which limits the use of mice in models of renal diseases. We aimed at simplifying the plasma clearance of iohexol in mice, using dried blood spot (DBS) sampling in order to reduce the amount of blood taken for analysis. GFR was measured simultaneously by a reference method in total blood—as described before—and tested method using DBS in fifteen male and six female C57BL/6J mice. Total blood extraction was 50 μL for the reference methods and 25μL for the tested methods, distributed in 5 samples. The agreement of GFR values between both methods was analyzed with the concordance correlation coefficient (CCC), total deviation index (TDI) and coverage probability (CP). The agreement between both methods was excellent, showing a TDI = 8.1%, which indicates that 90% of the GFR values obtained with DBS showed an error ranging from − 8 to + 8% of the reference method; a CCC of 0.996 (CI: 0.992), reflecting high precision and accuracy and a CP of 94 (CI: 83), indicating that 6% of the GFR values obtained with DBS had an error greater than 10% of the method in blood. So, both methods are interchangeable. DBS represent a major simplification of GFR measurement in mice. Also, DBS improves animal welfare by reducing the total blood required and refining the procedure.

scholarly journals Transcranial Evoked Potentials Can Be Reliably Recorded with Active Electrodes

2021 ◽  
Vol 11 (2) ◽  
pp. 145
Author(s):  
Marco Mancuso ◽  
Valerio Sveva ◽  
Alessandro Cruciani ◽  
Katlyn Brown ◽  
Jaime Ibáñez ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Magnetic Stimulation ◽  
Primary Motor Cortex ◽  
The Other ◽  
Concordance Correlation Coefficient ◽  
Concordance Correlation ◽  
Eeg Signals ◽  
Active Electrode ◽  
Viable Solution ◽  
Space Projection

Electroencephalographic (EEG) signals evoked by transcranial magnetic stimulation (TMS) are usually recorded with passive electrodes (PE). Active electrode (AE) systems have recently become widely available; compared to PE, they allow for easier electrode preparation and a higher-quality signal, due to the preamplification at the electrode stage, which reduces electrical line noise. The performance between the AE and PE can differ, especially with fast EEG voltage changes, which can easily occur with TMS-EEG; however, a systematic comparison in the TMS-EEG setting has not been made. Therefore, we recorded TMS-evoked EEG potentials (TEPs) in a group of healthy subjects in two sessions, one using PE and the other using AE. We stimulated the left primary motor cortex and right medial prefrontal cortex and used two different approaches to remove early TMS artefacts, Independent Component Analysis and Signal Space Projection—Source Informed Recovery. We assessed statistical differences in amplitude and topography of TEPs, and their similarity, by means of the concordance correlation coefficient (CCC). We also tested the capability of each system to approximate the final TEP waveform with a reduced number of trials. The results showed that TEPs recorded with AE and PE do not differ in amplitude and topography, and only few electrodes showed a lower-than-expected CCC between the two methods of amplification. We conclude that AE are a viable solution for TMS-EEG recording.

A repeated measures concordance correlation coefficient

2007 ◽  
Vol 26 (16) ◽  
pp. 3095-3113 ◽  
Author(s):  
Tonya S. King ◽  
Vernon M. Chinchilli ◽  
Josep L. Carrasco
Keyword(s):  
Correlation Coefficient ◽  
Repeated Measures ◽  
Concordance Correlation Coefficient ◽  
Concordance Correlation

A Comparison of Disease Assessment Methods for Southern Stem Rot of Peanut

2002 ◽  
Vol 29 (1) ◽  
pp. 66-71 ◽  
Author(s):  
S. L. Rideout ◽  
T. B. Brenneman ◽  
K. L. Stevenson
Keyword(s):  
Alternative Assessment ◽  
Disease Incidence ◽  
Sclerotium Rolfsii ◽  
Correlation Coefficients ◽  
Assessment Method ◽  
Assessment Methods ◽  
Alternative Methods ◽  
Stem Rot ◽  
Disease Assessment ◽  
Time Required

Abstract Southern stem rot (caused by the soilborne fungus Sclerotium rolfsii Sacc.) of peanut (Arachis hypogaea L.) traditionally has been assessed based on the percentage of infected 30.5-cm row segments, commonly referred to as disease incidence. Several alternative disease assessment methods were evaluated in four fungicide trials during the growing season (aboveground ratings) and immediately after peanut inversion (belowground ratings). Pearson's correlation coefficients compared disease assessments and yields for all trials. Across all disease assessment methods, belowground assessments at inversion showed a stronger correlation with yield than in-season aboveground assessments. Several of the alternative assessment methods showed a stronger negative correlation with yield than did the traditional disease incidence rating. However, none of the alternative methods were consistently more precise across all assessment dates and trials. There was a significant positive correlation between many of the alternative methods and the traditional disease incidence method. Furthermore, none of the alternative methods was better than the traditional method for detecting differences among fungicide treatments when subjected to ANOVA and subsequent Waller-Duncan mean separation tests (k-ratio = 100). Based on comparisons of the time required to assess disease intensity, the traditional disease assessment method was found to be the most time efficient method of those tested in this study.

scholarly journals Association between antimicrobial usage and resistance in Salmonella from poultry farms in Nigeria

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Abdurrahman Hassan Jibril ◽  
Iruka N. Okeke ◽  
Anders Dalsgaard ◽  
John Elmerdahl Olsen
Keyword(s):  
Treatment Outcome ◽  
Antimicrobial Resistance ◽  
Global Health ◽  
Human Medicine ◽  
Antimicrobial Use ◽  
Resistance Development ◽  
Use Patterns ◽  
Antimicrobial Usage ◽  
Poultry Farms ◽  
Reduction Strategies

Abstract Background Antimicrobial resistance (AMR) is a global health threat affecting treatment outcome in animals and humans. A pre-requisite for development of AMR reduction strategies is knowledge of antimicrobial use patterns, and how these affect resistance development. The aim of this study was to determine antimicrobial usage (AMU) and whether such usage was associated with AMR in Salmonella from poultry farms in Northwest Nigeria. Results Fifteen (37%) of antimicrobial products observed contained compounds that are of highest priority and critically important for human medicine. Broilers chicken consumed higher (28 ± 14 mg/kg active ingredients) amounts of antimicrobials compared to layers (13 ± 8 mg/kg) per week (p = 0.0009). Surprisingly, chickens raised under backyard system consumed higher amounts of antimicrobials (34 ± 7 mg/kg) than poultry in other systems (p = 0.02). High levels of resistance to tetracycline (58%), sulphonamides (65%), ciprofloxacin (46%) and gentamicin (42%) correlated with high farm level usage of these antimicrobials, and there was a strong correlation (r = 0.9) between farm usage and resistance of isolates to the same antimicrobials (p = 0.03). Conclusion High AMU, including use of highest priority critically important antimicrobials was observed at poultry farms in Northwest Nigeria. AMU correlated with high levels of resistance. Communication of prudent use of antimicrobials to farmers and regulation to obtain reduction in AMU should be a priority.

scholarly journals Estimation of coronary wave intensity analysis using noninvasive techniques and its application to exercise physiology

2016 ◽  
Vol 310 (5) ◽  
pp. H619-H627 ◽  
Author(s):  
Christopher J. Broyd ◽  
Sukhjinder Nijjer ◽  
Sayan Sen ◽  
Ricardo Petraco ◽  
Siana Jones ◽  
...  
Keyword(s):  
Coronary Circulation ◽  
Exercise Physiology ◽  
Left Ventricular ◽  
Wave Intensity ◽  
Concordance Correlation Coefficient ◽  
Intensity Analysis ◽  
Wave Intensity Analysis ◽  
Concordance Correlation ◽  
Decompression Wave ◽  
Pulsed Wave Doppler

Wave intensity analysis (WIA) has found particular applicability in the coronary circulation where it can quantify traveling waves that accelerate and decelerate blood flow. The most important wave for the regulation of flow is the backward-traveling decompression wave (BDW). Coronary WIA has hitherto always been calculated from invasive measures of pressure and flow. However, recently it has become feasible to obtain estimates of these waveforms noninvasively. In this study we set out to assess the agreement between invasive and noninvasive coronary WIA at rest and measure the effect of exercise. Twenty-two patients (mean age 60) with unobstructed coronaries underwent invasive WIA in the left anterior descending artery (LAD). Immediately afterwards, noninvasive LAD flow and pressure were recorded and WIA calculated from pulsed-wave Doppler coronary flow velocity and central blood pressure waveforms measured using a cuff-based technique. Nine of these patients underwent noninvasive coronary WIA assessment during exercise. A pattern of six waves were observed in both modalities. The BDW was similar between invasive and noninvasive measures [peak: 14.9 ± 7.8 vs. −13.8 ± 7.1 × 104 W·m−2·s−2, concordance correlation coefficient (CCC): 0.73, P < 0.01; cumulative: −64.4 ± 32.8 vs. −59.4 ± 34.2 × 102 W·m−2·s−1, CCC: 0.66, P < 0.01], but smaller waves were underestimated noninvasively. Increased left ventricular mass correlated with a decreased noninvasive BDW fraction ( r = −0.48, P = 0.02). Exercise increased the BDW: at maximum exercise peak BDW was −47.0 ± 29.5 × 104 W·m−2·s−2 ( P < 0.01 vs. rest) and cumulative BDW −19.2 ± 12.6 × 103 W·m−2·s−1 ( P < 0.01 vs. rest). The BDW can be measured noninvasively with acceptable reliably potentially simplifying assessments and increasing the applicability of coronary WIA.

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