Compressional-Puffing Pretreatment Enhances Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells

Iron may play an important role in Parkinson's disease (PD) since it can induce oxidative stress-dependent neurodegeneration. The objective of this study was to determine whether the iron chelator, phytic acid (IP6) can protect against 6-hydroxydopamine- (6-OHDA-) induced apoptosis in immortalized rat mesencephalic dopaminergic cells under normal and iron-excess conditions. Caspase-3 activity was increased about 6-fold after 6-OHDA treatment (compared to control; ) and 30 μmol/L IP6 pretreatment decreased it by 38% (). Similarly, a 63% protection () against 6-OHDA induced DNA fragmentation was observed with IP6 pretreatment. Under iron-excess condition, a 6-fold increase in caspase-3 activity () and a 42% increase in DNA fragmentation () with 6-OHDA treatment were decreased by 41% () and 27% (), respectively, with 30 μmol/L IP6. Together, our data suggest that IP6 protects against 6-OHDA-induced cell apoptosis in both normal and iron-excess conditions, and IP6 may offer neuroprotection in PD.

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Critical role of ASK1 in the 6-hydroxydopamine-induced apoptosis in human neuroblastoma SH-SY5Y cells

Journal of Neurochemistry ◽

10.1111/j.1471-4159.2006.03730.x ◽

2006 ◽

Vol 97 (1) ◽

pp. 234-244 ◽

Cited By ~ 83

Author(s):

Mingxing Ouyang ◽

Xun Shen

Keyword(s):

Critical Role ◽

Human Neuroblastoma ◽

Induced Apoptosis ◽

6 Hydroxydopamine

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Neuroprotective Effects of Methyl 3,4-dihydroxybenzoate Against H2O2-Induced Apoptosis in RGC-5 Cells

Journal of Pharmacological Sciences ◽

10.1254/jphs.13055fp ◽

2014 ◽

Vol 125 (1) ◽

pp. 51-58 ◽

Cited By ~ 13

Author(s):

Xing Zhou ◽

Chao-Fen Su ◽

Zheng Zhang ◽

Chen-Yu Wang ◽

Jin-Qi Luo ◽

...

Keyword(s):

Neuroprotective Effects ◽

Induced Apoptosis

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Icariin Prevents Amyloid Beta-Induced Apoptosis via the PI3K/Akt Pathway in PC-12 Cells

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2015/235265 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 12

Author(s):

Dongdong Zhang ◽

Zhe Wang ◽

Chenxia Sheng ◽

Weijun Peng ◽

Shan Hui ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Pc12 Cells ◽

Amyloid Beta ◽

Chinese Herb ◽

Protective Effects ◽

Amyloid Beta Protein ◽

Neuroprotective Effects ◽

Pi3k Inhibitor Ly294002 ◽

Induced Apoptosis

Icariin is a prenylated flavonol glycoside derived from the Chinese herbEpimedium sagittatumthat exerts a variety of pharmacological activities and shows promise in the treatment and prevention of Alzheimer’s disease. In this study, we investigated the neuroprotective effects of icariin against amyloid beta protein fragment 25–35 (Aβ25–35) induced neurotoxicity in cultured rat pheochromocytoma PC12 cells and explored potential underlying mechanisms. Our results showed that icariin dose-dependently increased cell viability and decreasedAβ25–35-induced apoptosis, as assessed by MTT assay and Annexin V/propidium iodide staining, respectively. Results of western blot analysis revealed that the selective phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 suppressed icariin-induced Akt phosphorylation, suggesting that the protective effects of icariin are associated with activation of the PI3K/Akt signaling pathway. LY294002 also blocked the icariin-induced downregulation of proapoptotic factors Bax and caspase-3 and upregulation of antiapoptotic factor Bcl-2 inAβ25–35-treated PC12 cells. These findings provide further evidence for the clinical efficacy of icariin in the treatment of Alzheimer’s disease.

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Tanshinone IIA exerts neuroprotective effects on hippocampus-dependent cognitive impairments in diabetic rats by attenuating ER stress-induced apoptosis

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2018.05.040 ◽

2018 ◽

Vol 104 ◽

pp. 530-536 ◽

Cited By ~ 15

Author(s):

Jian Chen ◽

Yanli Bi ◽

Lei Chen ◽

Qi Zhang ◽

Linhao Xu

Keyword(s):

Er Stress ◽

Cognitive Impairments ◽

Diabetic Rats ◽

Tanshinone Iia ◽

Neuroprotective Effects ◽

Induced Apoptosis

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Secoisolariciresinol Diglucoside Delays the Progression of Aging-Related Diseases and Extends the Lifespan of Caenorhabditis elegans via DAF-16 and HSF-1

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/1293935 ◽

2020 ◽

Vol 2020 ◽

pp. 1-13

Author(s):

Min Lu ◽

Lin Tan ◽

Xiao-Gang Zhou ◽

Zhong-Lin Yang ◽

Qing Zhu ◽

...

Keyword(s):

Caenorhabditis Elegans ◽

Body Movement ◽

Mrna Level ◽

Secoisolariciresinol Diglucoside ◽

Neuroprotective Effects ◽

Sesame Seeds ◽

C Elegans ◽

Age Related ◽

6 Hydroxydopamine ◽

And Oxidative Stress

Secoisolariciresinol diglucoside (SDG) is a phytoestrogen and rich in food flaxseed, sunflower seeds, and sesame seeds. Among the beneficial pharmacological activities of SDG on health, many are age related, such as anticancer, antidiabetes, antioxidant, and neuroprotective effects. Thus, we investigated if SDG had an effect on antiaging in Caenorhabditis elegans (C. elegans). Our results showed that SDG could extend the lifespan of C. elegans by up to 22.0%, delay age-related decline of body movement, reduce the lethality of heat and oxidative stress, alleviate dopamine neurodegeneration induced by 6-hydroxydopamine (6-OHDA), and decrease the toxicity of Aβ protein in C. elegans. SDG could increase the expression of the downstream genes of DAF-16, DAF-12, NHR-80, and HSF-1 at mRNA level. SDG could not extend the lifespan of mutants from genes daf-16, hsf-1, nhr-80, daf-12, glp-1, eat-2, and aak-2. The above results suggested that SDG might enhance the stress resistance, delay the progression of aging-related diseases, and extend the lifespan of C. elegans via DAF-16 and HSF-1.

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