scholarly journals Quantitative Evaluation of Twelve Major Components of Sulfur-Fumigated Astragali Radix with Different Durations by UPLC-MS

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2609 ◽  
Author(s):  
Xiaoyan Xing ◽  
Zhonghao Sun ◽  
Meihua Yang ◽  
Nailiang Zhu ◽  
Junshan Yang ◽  
...  
Keyword(s):  
Chemical Transformation ◽  
High Performance ◽  
Reference Sample ◽  
Flavonoid Glycosides ◽  
Bioactive Components ◽  
Astragaloside Iv ◽  
Astragali Radix ◽  
Global Quality ◽  
Liquid Chromatography Tandem Mass

In this study, an improved UPLC-MS (Ultra-high performance liquid chromatography-tandem mass spectrometry) method for simultaneously quantifying twelve major components belonging to two chemical types was developed and validated, and was applied to quantitatively compare the quality of sulfur-fumigated Astragali Radix of different durations and of the fresh reference sample. The results showed that the contents of triterpenes astragaloside III and astragaloside IV decreased moderately, while the flavonoids calycosin, formononetin, and 7,2′-dihydroxy-3′,4′-dimethoxyisoflavane decreased significantly. The corresponding flavonoid glycosides increased accordingly, which indicated the occurrence of chemical transformation of flavonoids and glycosides in the process of sulfur-fumigation. These transformations were further confirmed by the synthesis of flavonoid glycosides under simulated sulfur-fumigation circumstances. Furthermore, the sulfur-fumigated duration varied in proportion with the contents of compounds 7, 11, and 12. These results suggest that the established method was precise, accurate and sensitive enough for the global quality evaluation of sulfur-fumigated Astragali Radix. Further, sulfur-fumigation not only changes the proportions of bioactive components, but also causes chemical transformation in Astragali Radix.

scholarly journals Quantitative Evaluation of Twelve Major Components of Astragali Radix Sulfur-Fumigated with Different Durations by UPLC-MS

2018 ◽  
Author(s):  
Xing Xiaoyan ◽  
Sun Zhonghao ◽  
Yang Meihua ◽  
Zhu Nailiang ◽  
Yang Junshan ◽  
...  
Keyword(s):  
Chemical Transformation ◽  
Quality Evaluation ◽  
Reference Sample ◽  
Flavonoid Glycosides ◽  
Bioactive Components ◽  
Astragaloside Iv ◽  
Astragali Radix ◽  
Global Quality ◽  
Proportional Relationship

In this study, an improved UPLC-MS method for simultaneously quantifying twelve major components belonging to two chemical types was developed and validated, and was applied to quantitatively compare the quality of Astragali Radix sulfur-fumigated with different durations and the fresh reference sample. The results showed that the contents of triterpenes Astragaloside III and Astragaloside IV decreased moderately, while the flavonoids calycosin, formononetin, and 7,2'-dihydroxy-3',4'-dimethoxyisoflavane decreased significantly, and its corresponding flavonoid glycosides increased accordingly, which indicatied that the happening of chemical transformation of flavonoids and glycosides in the sulfur-fumigated process. These transformations were further confirmed by the the synthesis of flavonoid glycosides under the simulated sulphur-fumigation circumstances. Furthermore, the sulfur-fumigated duration had a proportional relationship with the contents of compounds 7, 11, and 12. All these results suggested that the established method was precise, accurate and sensitive enough for the global quality evaluation of sulfur-fumigated Astragali Radix, and sulfur-fumigation can not only change the proportions of bioactive components, but also cause the chemical transformation in the Astragali Radix.

The Correlation Between Bioactive Components of Fallopia multiflora Root and Environmental Factors

2010 ◽  
Vol 38 (03) ◽  
pp. 473-483 ◽  
Author(s):  
Han-Jing Yan ◽  
Zhi-Jian Fang ◽  
Jun Fu ◽  
Shi-Xiao Yu
Keyword(s):  
High Performance Liquid Chromatography ◽  
Environmental Factors ◽  
High Performance ◽  
Stepwise Regression ◽  
Bioactive Components ◽  
Average Temperature ◽  
Accumulated Temperature ◽  
Main Factor ◽  
Chinese Medicinal Plant

Fallopia multiflora (Thunb.) Harald, a traditional Chinese medicinal plant, is used in treating dizziness. In this study, the samples of F. multiflora from ten different locations were collected, and five bioactive components (2, 3, 5, 4′-tetrahydroxystilbene-2-O-β-D-glucoside, emodin, emodin-8-O-β-D-glucoside, physcion and physcion-8-O-β-D-glucoside) were quantified by high performance liquid chromatography. The correlations between 17 environmental factors and 5 bioactive components were analyzed. The results showed that the highest contents of bioactive components were in samples from Deqing, and the lowest in samples from Tianyang, which indicated that the quality of F. multiflora grown in Deqing was superior, while that grown in Tianyang was inferior. Emodin content was negatively correlated with the average temperature in January (p < 0.01) and the accumulated temperature (p < 0.01). Physician content was also negatively correlated with the average temperature in January (P < 0.01), the accumulated temperature (p < 0.05) and the organic matter (p < 0.05). However, emodin was positively correlated with the soil available K (p < 0.05) and Zn (p < 0.01) . The results of stepwise regression showed that the accumulated temperature was the main factor influencing the contents of emodin and physcion. However, none of the environmental factors had significant correlation with 2, 3, 5, 4′-tetrahydroxystilbene-2-O-β-D-glucoside, emodin-8- O-β-D-glucoside and physcion-8-O-β-D-glucoside. In conclusion, some environmental factors have significant influence on the content of dissociated anthraquinones, while some have no influence on that of combined anthraquinones.

scholarly journals SIMULTANEOUS ANALYSIS OF THE BIOACTIVE COMPONENTS OF AN EXTRACT OF YEONGGYECHULGAM-TANG, A TRADITIONAL HERBAL PRESCRIPTION, USING HPLC–DAD

2018 ◽  
Vol 15 (2) ◽  
pp. 88
Author(s):  
Chang-Seob Seo ◽  
Hyeun-Kyoo Shin
Keyword(s):  
Analytical Method ◽  
High Performance ◽  
Correlation Coefficients ◽  
Simultaneous Analysis ◽  
Bioactive Components ◽  
Column Temperature ◽  
Relative Standard ◽  
Array Detection ◽  
Lower Limits

Background: Yeonggyechulgam-tang (YGCGT) is a well-known classic herbal formula and has been used clinically in Korea for the treatment of chest congestion. High-performance liquid chromatography (HPLC) analytical method coupled with diode-array detection (DAD) was performed for the simultaneous analysis of eight bioactive components, liquiritin apioside, liquiritin, coumarin, liquiritigenin, cinnamic acid, cinnamaldehyde, glycyrrhizin, and atractylenolide III in a YGCGT decoction. Materials and Methods: For simultaneous analysis using HPLC, the eight components were separated using a Phenomenex Gemini C18 column (250 mm  4.6 mm; particle size 5 m) eluted with a gradient of 0.1% (v/v) aqueous trifluoroacetic acid and acetonitrile at 1.0 mL/min. The column temperature and injection volume were 40C and 10 L. Results: Correlation coefficients of the eight compounds ranged between 0.9996 and 1.0000. The lower limits of detection and quantification of the analytes were 0.01–0.09 and 0.02–0.28 g/mL, respectively. Recovery of the eight compounds was 97.63–102.70% and the relative standard deviation (RSD) was less than 3.00%. The RSDs of intra and interday precision were 0.06–2.07% and 0.02–1.95%, respectively. The amounts of the eight compounds in a lyophilized YGCGT were in the range 0.18 to 10.34 mg/g. Conclusion: The optimized and validated HPLC analytical method used in the present study is expected to be useful for evaluation the quality of YGCGT decoctions or related herbal prescriptions.

scholarly journals Cell Chromatography-Based Screening of the Active Components in Buyang Huanwu Decoction Promoting Axonal Regeneration

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Xiangli Yan ◽  
Shengxin Wang ◽  
Aiming Yu ◽  
Xiao Shen ◽  
Haozhen Zheng ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Axonal Regeneration ◽  
High Performance ◽  
Potential Role ◽  
Glucose Deprivation ◽  
Protective Effects ◽  
Bioactive Components ◽  
Active Components ◽  
Buyang Huanwu Decoction ◽  
Liquid Chromatography Tandem Mass

Buyang Huanwu decoction (BHD), a popular formulation prescribed in traditional Chinese medicine (TCM) for the treatment of ischemic stroke, has been reported to have a potential role in promoting axonal regeneration. The purpose of the study was to screen and identify bioactive compounds from BHD using live PC12 cells coupled with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Using this approach, we successfully identified six bioactive components from BHD. These components have protective effects on oxygen-glucose deprivation/reperfusion (OGD/R) injury to PC12 cells. Furthermore, calycosin-7-D-glucoside (CG) and formononetin-7-O-β-D-glucoside (FG) could upregulate the protein expression of growth-associated protein 43 (GAP-43) and brain-derived neurotrophic factor (BDNF). This study suggests that living cells combined with HPLC-MS/MS can be used for the screening of active ingredients in TCMs.

scholarly journals Measurement of Quality of Life I. A Methodological Framework

2003 ◽  
Vol 3 ◽  
pp. 950-961 ◽  
Author(s):  
Soren Ventegodt ◽  
Jorgen Hilden ◽  
Joav Merrick
Keyword(s):  
Quality Of Life ◽  
Human Life ◽  
Reference Sample ◽  
Life Questionnaire ◽  
Methodological Framework ◽  
Smoking During Pregnancy ◽  
Quality Of Life Questionnaire ◽  
Global Quality ◽  
Global Quality Of Life

Despite the widespread acceptance of quality of life (QOL) as the ideal guideline in healthcare and clinical research, serious conceptual and methodological problems continue to plague this area. In an attempt to remedy this situation, we propose seven criteria that a quality-of-life concept must meet to provide a sound basis for investigation by questionnaire. The seven criteria or desiderata are: (1) an explicit definition of quality of life; (2) a coherent philosophy of human life from which the definition is derived; (3) a theory that operationalizes the philosophy by specifying unambiguous, nonoverlapping, and jointly exhaustive questionnaire items; (4) response alternatives that permit a fraction-scale interpretation; (5) technical checks of reproducibility; (6) meaningfulness to investigators, respondents, and users; and (7) an overall aesthetic appeal of the questionnaire. These criteria have guided the design of a validated 5-item generic, global quality-of-life questionnaire (QOL5), and a validated 317-item generic, global quality-of-life questionnaire (SEQOL), administered to a well-documented birth cohort of 7,400 Danes born in 1959�1961, as well as to a reference sample of 2,500 Danes. Presented in outline, the underlying integrative quality-of-life (IQOL) theory is a meta-theory. To illustrate the seven criteria at work, we show the extent to which they are satisfied by one of the eight component theories. Next, two sample results of our investigation are presented: satisfaction with one's sex life has the expected covariation with one's quality of life, and so does mother's smoking during pregnancy, albeit to a much smaller extent. It is concluded that the methodological framework presented has proved helpful in designing a questionnaire that is capable of yielding acceptably valid and reliable measurements of global and generic quality of life.

scholarly journals Major bioactive chemical compounds in Astragali Radix samples from different vendors vary greatly

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0254273
Author(s):  
Bijay Kafle ◽  
Jan P. A. Baak ◽  
Cato Brede
Keyword(s):  
Standard Addition ◽  
Bioactive Molecules ◽  
European Medicines Agency ◽  
The European Union ◽  
Ammonia Treatment ◽  
Astragali Radix ◽  
Liquid Chromatography Tandem Mass ◽  
Powder Samples ◽  
Extraction Processes

The worldwide traditional Chinese medicine (TCM) herbs sales figures have increased considerably to 50 billion US$ (2018). Astragali Radix (AR) is amongst the most often sold TCM herbs; sales in the European Union (EU) need European Medicines Agency (EMA) approval. However, comparisons of characteristic bioactive molecules concentrations in AR from different EU vendors are lacking. This study uses liquid chromatography-tandem mass spectrometry (LC-MS/MS) with standard addition to evaluate the influence of different sample and preparation types and ammonia treatment on bioactive molecules concentrations in AR. We also compare AR samples from different EU-vendors. Astragaloside IV (AG-IV), ononin and calycosin 7-O-β-D-glucoside concentrations were higher in root powder samples when extracted with boiled water than with ultrasonication using 70% methanol. AG-IV content was by far the highest in granulates from vendor 1 (202 ± 35 μg/g) but very low in hydrophilic concentrates from vendor 1 (32 ± 7 μg/g) and granulates from vendor 4 (36 ± 3 μg/g). Ammonia-treatment significantly increased AG-IV concentrations in all samples (e.g., to 536 ± 178 μg/g in vendor 1 granulates). Comparable effects were found for most other bioactive molecules. AG-IV and other bioactive molecules concentrations differed strongly depending on sample types, extraction processes, ammonia treatment-or-not and especially between different vendors samples. Ammonia-treatment is debatable, as it is supposed to convert other astragalosides, to AG-IV. The results indicate that routine quantitative analysis of major bioactive compounds present in AR, helps in quality control of AR and to guarantee the quality of commercial products.

scholarly journals Profiling of Phytochemicals in Tissues from Sclerocarya birrea by HPLC-MS and Their Link with Antioxidant Activity

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Daniela Russo ◽  
Owen Kenny ◽  
Thomas J. Smyth ◽  
Luigi Milella ◽  
Mohammad B. Hossain ◽  
...  
Keyword(s):  
High Performance ◽  
Antioxidant Activities ◽  
Flavonoid Glycosides ◽  
Tandem Mass ◽  
Leaf Extracts ◽  
Root Extracts ◽  
Sclerocarya Birrea ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
First Time

High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed to investigate the differences in phytochemicals in roots, bark, and leaf of Sclerocarya birrea (marula) for methanol and water extracts that exhibited the best antioxidant activities. As many as 36 compounds were observed in the extracts of these tissues of which 27 phenolic compounds were tentatively identified. The HPLC-MS/MS results showed flavonoid glycosides were prominent in leaf extracts while the galloylated tannins were largely in bark and root extracts. Four flavonoid glycosides that were reported for the first time in the marula leaf have been identified. The HPLC-MS/MS studies also illustrated different degrees (highest degree = 3) of oligomerisation and galloylation of tannins in the bark and root extracts.

Simultaneous Analysis of Vanillin and Coumarin in Mangrove Plants and Commercial Food Products Using UPLC-ESI-MS/MS

2020 ◽  
Vol 16 (6) ◽  
pp. 768-777 ◽  
Author(s):  
Nilesh Lakshman Dahibhate ◽  
Devendra Kumar ◽  
Kundan Kumar
Keyword(s):  
High Performance ◽  
Structural Information ◽  
Multiple Reaction Monitoring ◽  
Food Products ◽  
High Price ◽  
Fragmentation Pattern ◽  
Limit Of Quantification ◽  
Mangrove Species ◽  
Liquid Chromatography Tandem Mass

Background: Vanillin is a key constituent of natural vanilla. Usage of natural vanilla is affected due to its high price and limited supply, which leads to the use of artificial vanilla flavoring substances. Coumarin is a commonly encountered adulterant in beverage, food, and cosmetics as a flavoring and fragrance enhancer. However, coumarin has been banned for use as a food additive due to its toxic effects. To comply with the quality of vanillin in food and food products needs to be ensured. Methods: A rapid, simple and selective analytical method has been developed and validated using ultra-high performance liquid chromatography-tandem mass spectrometry for quantitative analysis of vanillin and coumarin. We also optimized fragmentation pattern of these metabolites while increasing collision energy to elucidate its structural information. The suitability and robustness of the method was checked by Zorbax Eclipse XDB C8 column (4.6 × 150 mm, 5 μm) using mobile phase comprising of methanol (A) and water with 0.1% formic acid (B) (90:10) with a flow rate 200 μL/min. The separation was achieved within 4.2 min with total run time of 5.0 min. The analysis was done by multiple reaction monitoring using 153/93 and 147/91 pair transition in positive electrospray ionization for vanillin and coumarin respectively. Results: The lower limit of quantification of vanillin and coumarin was 0.39 ng/mL and 3.9 ng/mL respectively. The intra and inter-day precisions for vanillin and coumarin were lower than 8.87 and 8.62 whereas, accuracy was within ± 2.13 and ± 1.53 respectively. The vanillin and coumarin was found to be stable under the examined conditions. This method was successfully applied for quantification of vanillin and coumarin in mangrove species and commercial food products. Conclusion: The described method and fragmentation pattern could be useful to direct confirmation and quality monitoring of a commercial food products assimilated with vanillin.

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