scholarly journals Gastroprotection of Calein D against Ethanol-Induced Gastric Lesions in Mice: Role of Prostaglandins, Nitric Oxide and Sulfhydryls

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 622 ◽  
Author(s):  
María Sánchez-Mendoza ◽  
Yaraset López-Lorenzo ◽  
Leticia Cruz-Antonio ◽  
Audifás-Salvador Matus-Meza ◽  
Yolanda Sánchez-Mendoza ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Methyl Ester ◽  
Oral Treatment ◽  
Control Group ◽  
Peptic Ulcers ◽  
Gastric Lesions ◽  
Gastroprotective Activity ◽  
Reference Drug ◽  
Sulfhydryl Compounds

Peptic ulcers are currently treated with various drugs, all having serious side effects. The aim of this study was to evaluate the gastroprotective activity of calein D (from Calea urticifolia), a sesquiterpene lactone with a germacrane skeleton. Gastric lesions were induced in mice by administering ethanol (0.2 mL) after oral treatment with calein D at 3, 10 and 30 mg/kg, resulting in 13.15 ± 3.44%, 77.65 ± 7.38% and 95.76 ± 2.18% gastroprotection, respectively, to be compared with that of the control group. The effect found for 30 mg/kg of calein D was not reversed by pretreatment with NG-nitro-l-arginine methyl ester (l-NAME, 70 mg/kg, ip), indomethacin (10 mg/kg, sc) or N-ethylmaleimide (NEM, 10 mg/kg, sc). Hence, the mechanism of action of calein D does not involve NO, prostaglandins or sulfhydryl compounds. Calein D was more potent than carbenoxolone, the reference drug. The findings for the latter are in agreement with previous reports.

Gastroprotective effects of goniothalamin against ethanol and indomethacin-induced gastric lesions in rats: Role of prostaglandins, nitric oxide and sulfhydryl compounds

2014 ◽  
Vol 224 ◽  
pp. 206-212 ◽  
Author(s):  
Débora Barbosa Vendramini-Costa ◽  
Karin Maia Monteiro ◽  
Leilane Hespporte Iwamoto ◽  
Michelle Pedroza Jorge ◽  
Sirlene Valério Tinti ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Gastric Lesions ◽  
Sulfhydryl Compounds

Gastroprotective activity of Pradosia huberi on experimentally induced gastric lesions in rodents: Role of endogenous sulphydryls and nitric oxide

2005 ◽  
Vol 101 (1-3) ◽  
pp. 61-67 ◽  
Author(s):  
Hélio Kushima ◽  
Clélia Akiko Hiruma-Lima ◽  
Maria Aparecida Santos ◽  
Elizabeth Viana ◽  
Márlia Coelho-Ferreira ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Gastric Lesions ◽  
Gastroprotective Activity ◽  
Experimentally Induced

scholarly journals Gastroprotective Activity of Sterculia striata A. St. Hil. & Naudin (Malvaceae) in Rodents

2012 ◽  
Vol 67 (3-4) ◽  
pp. 163-171
Author(s):  
Joubert A. Sousa ◽  
Irisdalva S. Oliveira ◽  
Francilene V. Silva ◽  
Danielly A. Costa ◽  
Mariana H. Chaves ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Methyl Ester ◽  
Gastric Secretion ◽  
Catalase Activity ◽  
Gastric Lesion ◽  
Ischemia Reperfusion ◽  
Ethanolic Extract ◽  
Antioxidant Compounds ◽  
Gastric Lesions ◽  
Gastroprotective Activity

The Sterculia striata ethanolic extract (Ss-EtOH) inhibited gastric lesions induced by ethanol, HCl/ethanol, and ischemia/reperfusion, but not those induced by indomethacin, and did not alter the gastric secretion. Ss-EtOH restored the catalase activity and content of nonprotein sulfhydryl groups in the stomach of mice treated with ethanol. The gastroprotection induced by Ss-EtOH in the ethanol-induced gastric lesion model was abolished by NG-nitro- L-arginine methyl ester (L-NAME) pretreatment, suggesting the involvement of nitric oxide and antioxidant compounds, but not prostaglandins, in this activity. Lupeol obtained from Ss-EtOH promoted gastroprotection as well as the extract at the same dose, and it must therefore contribute to the observed effects

scholarly journals Role of Prostaglandins, Nitric oxide, Sulfhydryls and Capsaicin-sensitive Neurons in Gastroprotection of Stigmasterol and β-Sitosterol

2008 ◽  
Vol 3 (4) ◽  
pp. 1934578X0800300
Author(s):  
María Elena Sánchez-Mendoza ◽  
Jesús Arrieta ◽  
Andrés Navarrete
Keyword(s):  
Nitric Oxide ◽  
Methyl Ester ◽  
Sensory Neurons ◽  
Tween 80 ◽  
Mucosal Damage ◽  
Gastric Mucosal Damage ◽  
Gastroprotective Activity ◽  
Model Drug ◽  
Dose Dependent

In this investigation the gastroprotective activity of stigmasterol and β-sitosterol was evaluated. Gastric mucosal damage was induced in rats by intragastric ethanol (1 mL/rat). Rats treated orally with stigmasterol suspended in Tween 80 at 10, 30, 100 and 300 mg kg−1 showed 26.2, 39.6, 58.3 and 70.7% gastroprotection, respectively. β-Sitosterol at 10, 30,100 and 300 mg kg−1 showed 21.6, 42.5, 48.5 and 71.2% gastroprotection, correspondingly. The gastroprotection observed at 30 mg kg−1 for stigmasterol and β-sitosterol was attenuated in rats pretreated with indomethacin, (10 mg kg−1, s. c.), NG-nitro-L-arginine methyl ester (L-NAME, 70 mg kg−1, i. p.) and capsaicin (125 mg kg−1, s. c), suggesting that the gastroprotective mechanism of these sterols involves, at least in part, the participation of prostaglandins, nitric oxide (NO) and capsaicin-sensitive sensory neurons (CPSN). The gastroprotection of β-sitosterol was also attenuated by the pretreatment with N-ethylmaleimide (NEM, 10 mg kg−1, s. c.) indicating that endogenous sulfrydryls may be involved in the gastrorpotection of this compound. Carbenoxolone was used as a gastroprotective model drug and showed a dose-dependent gastroprotective effect (25.7, 33.6 and 88.3% of gastroprotection, at 3, 10 and 30 mg kg−1, respectively). The partial participation of PGs, sulfhydryls and NO was observed in the gastroprotective mechanism of carbenoxolone.

scholarly journals Gastroprotective Effect of Juanislamin on Ethanol-Induced Gastric Lesions in Rats: Role of Prostaglandins, Nitric Oxide and Sulfhydryl Groups in the Mechanism of Action

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2246
Author(s):  
María Elena Sánchez-Mendoza ◽  
Yaraset López-Lorenzo ◽  
Leticia Cruz-Antonio ◽  
Arturo Cruz-Oseguera ◽  
Jazmín García-Machorro ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Mechanism Of Action ◽  
Gastrointestinal Disorder ◽  
Sulfhydryl Groups ◽  
Dependent Manner ◽  
Gastric Lesions ◽  
Reference Drug ◽  
Ulcer Disease ◽  
Gastroprotective Effect

Peptic ulcer disease, the most common gastrointestinal disorder, is currently treated with several types of drugs, but all have severe side effects. The aim of the present study was to evaluate the gastroprotective activity of juanislamin, isolated from Calea urticifolia, in a rat model of ethanol-induced gastric lesions. Thirty minutes after orally administering a given dose of juanislamin (from 1 to 30 mg/kg) or carbenoxolone (the reference drug, at 1–100 mg/kg) to rats, 1 mL of ethanol was applied, and the animals were sacrificed 2 h later. The stomachs were removed and opened to measure the total area of lesions in each. To examine the possible participation of prostaglandins, nitric oxide and/or sulfhydryl groups in the mechanism of action of juanislamin, the rats received indomethacin, NG-Nitro-l-arginine methyl ester hydrochloride (l-NAME) or N-ethylmaleimide pretreatment, respectively, before being given juanislamin and undergoing the rest of the methodology. Juanislamin inhibited gastric lesions produced by ethanol in a non-dose-dependent manner, showing the maximum gastroprotective effect (100%) at 10 mg/kg. The activity of juanislamin was not modified by pretreatment with indomethacin, l-NAME or N-ethylmaleimide. In conclusion, juanislamin protected the gastric mucosa from ethanol-induced damage, and its mechanism of action apparently does not involve prostaglandins, nitric oxide or sulfhydryl groups.

scholarly journals Phytochemical analysis and gastroprotective activity of an olive leaf extract

2009 ◽  
Vol 74 (4) ◽  
pp. 367-377 ◽  
Author(s):  
Dragana Dekanski ◽  
Snezana Janicijevic-Hudomal ◽  
Vanja Tadic ◽  
Goran Markovic ◽  
Ivana Arsic ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Defense Mechanism ◽  
Mucosal Injury ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Gastric Lesions ◽  
Olive Leaf ◽  
Gastroprotective Activity ◽  
Reference Drug ◽  
Olive Leaf Extract

Some medicinal features of olive leaf have been known for centuries. It has been traditionally used as an antimicrobial and to prevent and treat diabetes mellitus and heart disease. Whether olive leaf, a natural antioxidant, influences the gastric defense mechanism and exhibits gastroprotection against experimentally-induced gastric lesions remains unknown. In this study, the content of total phenols, total flavonoids and tannins in olive leaf extract (OLE) were determined. Seven phenolic compounds were identified and quantified (oleuropein, caffeic acid, luteolin, luteolin-7-O-glucoside, apigenin-7-O-glucoside, quercetin, and chryseriol). Furthermore, the protective activity of the OLE in gastric mucosal injury induced by a corrosive concentration of ethanol was investigated. In relation to the control group, pretreatment with OLE (40, 80 and 120 mg kg-1) significantly (p < 0.001) attenuated the gastric lesions induced by absolute ethanol. The protective effect of the OLE was similar to that obtained with a reference drug, ranitidine. The results obtained indicate that OLE possesses significant gastroprotective activity, and that the presence of compounds with antioxidative properties would probably explain this effect.

scholarly journals Role of Nitric Oxide in Regulation of Brain Stem Circulation during Hypotension

1997 ◽  
Vol 17 (10) ◽  
pp. 1089-1096 ◽  
Author(s):  
Kazunori Toyoda ◽  
Kenichiro Fujii ◽  
Setsuro Ibayashi ◽  
Tetsuhiko Nagao ◽  
Takanari Kitazono ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Brain Stem ◽  
Topical Application ◽  
Basilar Artery ◽  
Group Versus ◽  
Control Group ◽  
Cranial Window ◽  
Severe Hypotension ◽  
The Brain

We tested the hypothesis that nitric oxide (NO) plays a role in CBF autoregulation in the brain stem during hypotension. In anesthetized rats, local CBF to the brain stem was determined with laser-Doppler flowmetry, and diameters of the basilar artery and its branches were measured through an open cranial window during stepwise hemorrhagic hypotension. During topical application of 10−5 mol/L and 10−4 mol/L Nω-nitro-L-arginine (L-NNA), a nonselective inhibitor of nitric oxide synthase (NOS), CBF started to decrease at higher steps of mean arterial blood pressure in proportion to the concentration of L-NNA in stepwise hypotension (45 to 60 mm Hg in the 10−5 mol/L and 60 to 75 mm Hg in the 10−4 mol/L L-NNA group versus 30 to 45 mm Hg in the control group). Dilator response of the basilar artery to severe hypotension was significantly attenuated by topical application of L-NNA (maximum dilatation at 30 mm Hg: 16 ± 8% in the 10−5 mol/L and 12 ± 5% in the 10−4 mol/L L-NNA group versus 34 ± 4% in the control group), but that of the branches was similar between the control and L-NNA groups. Topical application of 10−5 mol/L 7-nitro indazole, a selective inhibitor of neuronal NOS, did not affect changes in CBF or vessel diameter through the entire pressure range. Thus, endothelial but not neuronal NO seems to take part in the regulation of CBF to the the brain stem during hypotension around the lower limits of CBF autoregulation. The role of NO in mediating dilatation in response to hypotension appears to be greater in large arteries than in small ones.

Role of NO in goat basal cerebral circulation and after vasodilatation to hypercapnia or brief ischemias

1993 ◽  
Vol 265 (6) ◽  
pp. R1410-R1415 ◽  
Author(s):  
G. Dieguez ◽  
J. L. Garcia ◽  
N. Fernandez ◽  
A. L. Garcia-Villalon ◽  
L. Monge ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Heart Rate ◽  
Methyl Ester ◽  
Cerebral Circulation ◽  
Systemic Arterial Pressure ◽  
Nitric Oxide Production ◽  
Hemodynamic Effects ◽  
Arterial Pco2 ◽  
Different Levels

The role of nitric oxide (NO) in the cerebral circulation under basal conditions and after vasodilatation to hypercapnia or reactive hyperemias was studied in 17 anesthetized goats. The intravenous administration of NG-nitro-L-arginine methyl ester (L-NAME, 3-4 or 8-10 mg/kg), an inhibitor of nitric oxide production, reduced middle cerebral artery (MCA) flow (electromagnetically measured) by 19 and 30% and increased systemic arterial pressure by 21 and 26%, respectively, whereas heart rate did not significantly change; MCA resistance increased by 48 and 86%, respectively. These hemodynamic effects were reversed by L-arginine (200-300 mg/kg iv; 5 goats). Different levels of hypercapnia (PCO2 of 30-35, 40-45, and 55-65 mmHg) (12 goats) produced arterial PCO2-dependent increases in MCA flow that were similar under control and L-NAME treatment. Graded cerebral hyperemia occurred after 5, 10, and 20 s of MCA occlusion in 5 goats, but its magnitude was decreased during L-NAME treatment. It suggests that, in the cerebral circulation, nitric oxide 1) produces a basal vasodilator tone and 2) is probably not involved in the vasodilatation to hypercapnia but may mediate hyperemic responses after short brain ischemias.

scholarly journals Role of insulin during the development of oligofructose (OF)-induced equine laminitis

2015 ◽  
Vol 59 (2) ◽  
pp. 303-309
Author(s):  
Renli Jiang ◽  
Li Gao ◽  
Guanying Wang ◽  
Xinran Li ◽  
Yue Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Glucose Oxidase ◽  
Negative Correlation ◽  
Significant Negative Correlation ◽  
Control Group ◽  
Endothelin 1 ◽  
Limulus Amoebocyte Lysate ◽  
And Control ◽  
Over Time

Abstract Horses (n = 20) were divided into 2 groups: oligofructose (OF)-induced equine laminitis group (group OF; n = 11) which received 10 g/kg b.w. of OF dissolved in 4 L water via nasogastric intubation, and control group (NS; n = 9) which received 4 L of saline. Blood was collected at 4 h intervals over 72 h study period and analysed by ELISA, kinetic limulus amoebocyte lysate assay, and glucose-oxidase methods. The level of insulin changed significantly in horses which received OF (P < 0.01); there was a significant negative correlation between the level of adiponectin and insulin over time. The results suggested that insulin may play an important role in the development of OF-induced equine laminitis by altering the level of endothelin-1 and nitric oxide.

Role of endothelium-derived nitric oxide in control of renal microvasculature in aging male rats

1993 ◽  
Vol 265 (5) ◽  
pp. R1126-R1131 ◽  
Author(s):  
J. F. Reckelhoff ◽  
R. D. Manning
Keyword(s):  
Nitric Oxide ◽  
Oral Treatment ◽  
Renal Hemodynamics ◽  
Male Rats ◽  
Glomerular Capillary ◽  
Old Rats ◽  
Renal Microvasculature ◽  
Glomerular Hemodynamics ◽  
Renal Vascular

The objective of this study was to evaluate the role of nitric oxide (NO) in the regulation of whole kidney and glomerular hemodynamics during aging. After 2 wk of oral treatment with N-nitro-L-arginine methyl ester (L-NAME; 4.5 mg.kg body wt-1.day-1) to inhibit NO synthesis, male rats, aged 3-5, 13-15, and 21-24 mo, were studied by micropuncture. Blood pressure increased by 50% in old (21-24 mo) rats with L-NAME but only 20-30% in the two younger groups. With L-NAME, renal vascular resistance increased fivefold in old rats but only twofold in younger groups. Glomerular capillary pressure increased 20-30% in younger L-NAME rats and 60% in older rats. Afferent and efferent resistances increased dramatically, and the glomerular capillary ultrafiltration coefficient decreased in all L-NAME-treated rats but most strikingly in the 21- to 24-mo-old group. Acute infusion of L-arginine significantly attenuated the effects of NO synthase inhibition on arterial pressure and renal hemodynamics in both young and old rats. This study confirms that NO synthesis blockade has a greater effect on renal hemodynamics in aging rats and implies that NO may play a progressively more important role in controlling renal function with advancing age.

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