scholarly journals Simultaneous Determination of Six Isoflavones from Puerariae Lobatae Radix by CPE-HPLC and Effect of Puerarin on Tyrosinase Activity

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 344 ◽  
Author(s):  
Limin Qu ◽  
Ke Song ◽  
Qi Zhang ◽  
Jie Guo ◽  
Juan Huang
Keyword(s):  
High Performance ◽  
Equilibrium Temperature ◽  
Extraction Yield ◽  
Tyrosinase Activity ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Triton X ◽  
Diphenolase Activity

Tyrosinase inhibitors with excellent inhibitory activities and lower side effects have promising applications in the fields of medicine, agriculture, food sciences and cosmetics. In this study, a method for simultaneous separation and determination of six target compounds (puerarin, daidzin, genistein, daidzein, genistin, and formononetin) in Puerariae Lobatae Radix was established by cloud point extraction (CPE) and concentration combined with high performance liquid chromatography (HPLC). To achieve high extraction yields, an ultrasound-assisted extraction method was developed based on a salt-modified Triton X-100 system. The optimal extraction conditions are: surfactant Triton X-100 concentration 0.07 g/mL, liquid-solid ratio 80:1 (mL/g), NaCl addition amount 0.6 g, equilibrium time 40 min, equilibrium temperature 70 °C. Under the optimal conditions, the total maximum extraction yield of the six target isoflavones reached 8.92 mg/g. Using l-tyrosine and l-dopa as substrates, the effects of puerarin on the monophenolase and diphenolase activity of tyrosinase activity were investigated by the enzyme kinetics method. The results showed that puerarin inhibited monophenolase activity with an IC50 of 0.537 mg/mL and activated diphenolase activity. The inhibition type of puerarin on monophenolase and the activation type of puerarin on diphenolase were analyzed by Lineweaver-Burk plots which show that puerarin showed mixed inhibition on monophenolase and mixed activation on diphenolase. Therefore, puerarin can be used as both a tyrosinase inhibitor and a tyrosinase activator.

QuEChERS with ultrasound-assisted extraction combined with high-performance liquid chromatography for the determination of 16 polycyclic aromatic hydrocarbons in sediment

2021 ◽  
Author(s):  
Ling Wu ◽  
Qiurong He ◽  
Jing Zhang ◽  
Yongxin Li ◽  
Weiqing Yang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Aromatic Hydrocarbons ◽  
High Performance ◽  
Ultrasound Assisted Extraction ◽  
Sediment Samples ◽  
Assisted Extraction ◽  
Polycyclic Aromatic ◽  
Ultrasound Assisted

Abstract Background Polycyclic aromatic hydrocarbons (PAHs) have attracted worldwide attention due to their carcinogenic, teratogenic and mutagenic effects, environmental persistence and bioaccumulation characteristics. Therefore, the sensitive, reliable and rapid detection of PAHs in sediment is of great importance. Objective To develop a high-performance liquid chromatography (HPLC) with fluorescence and ultraviolet detection after QuEChERS treatment for simultaneous determination of 16 U.S. Environmental Protection Agency priority PAHs in sediment samples. Methods The samples were ultrasonically extracted with acetone and then the supernatant was purified with a modified QuEChERS method. After centrifugation, the supernatant was injected into the HPLC system for analysis. The separation was accomplished on a ZORBAX Eclipse PAH column (150 × 4.6 mm, 3.5 μm) and the column temperature was set at 30 °C. The flow rate of the mobile phase consisting of water and acetonitrile in gradient elution mode was fixed at 0.9 mL/min. Detection was conducted on an ultraviolet detector and a fluorescence detector simultaneously. The qualitative analysis was based on retention time and the quantification was based on standard curves. Results Under the optimal conditions, this method showed good linearities in the range of 10–200 μg/L with correlation coefficients greater than 0.9993. The method had the limits of detection (LODs) ranging from 0.00108 to 0.314 ng/g. The mean recoveries ranged from 78.4 to 117% with the intra-day and inter-day relative standard deviations (RSDs) of 0.592–10.7 and 1.01–13.0%, respectively. The proposed method was successfully applied to the detection of 16 PAHs in sediment samples collected from the Funan River in Chengdu, China with the total contents of 431 to 2143 ng/g·dw. Conclusions The established method is simple, rapid, environment-friendly and cost- effective. It can be applied to the analysis of 16 PAHs in sediment samples. Highlights A method of QuEChERS with ultrasound-assisted extraction combined with HPLC has been established for the analysis of 16 PAHs in sediment samples and the proposed method has been successfully applied to the analysis PAHs in real sediment samples.

Determination of the Isoflavone Genistein in Soybeans by High-Performance Liquid Chromatography Following Cloud Point Extraction

2012 ◽  
Vol 95 (3) ◽  
pp. 845-849 ◽  
Author(s):  
Mohammad Mirzaei ◽  
Atieh Karimi Naeini ◽  
Mansoureh Behzadi
Keyword(s):  
Cloud Point ◽  
High Performance ◽  
Cloud Point Extraction ◽  
Uv Detector ◽  
Experimental Conditions ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Replicate Measurements

Abstract A simple and sensitive method has been developed for preconcentration and determination of genistein in soybeans. This method is based on cloud point extraction (CPE) of genistein from soybeans using ethylene glycol monoalkyl ether (Genapol X-080) as a nonionic surfactant. The concentration of extracted genistein was determined by HPLC with a UV detector. Optimum experimental conditions were established. With 5% Genapol X-080 (v/v), a liquid/solid ratio of 25:1 mL/g, and ultrasonic-assisted extraction at 40°C for 45 min, the extraction percentage of genistein reached its highest value. The preconcentration factor for genistein was about 16.5. The RSD for seven replicate measurements and the LOD were ±4.45% and 15.0 ng/mL, respectively. CPE is simple, inexpensive, and suitable for extraction of genistein from soybean. It uses environmentally friendly surfactants and offers a convenient alternative to more conventional extraction systems.

scholarly journals Enhancement of Solasodine Extracted from Fruits of Solanum nigrum L. by Microwave-Assisted Aqueous Two-Phase Extraction and Analysis by High-Performance Liquid Chromatography

Molecules ◽  
2019 ◽  
Vol 24 (12) ◽  
pp. 2294 ◽  
Author(s):  
Li Lin ◽  
Wen Yang ◽  
Xing Wei ◽  
Yi Wang ◽  
Li Zhang ◽  
...  
Keyword(s):  
Extraction Method ◽  
High Performance ◽  
Extraction Yield ◽  
Solanum Nigrum ◽  
Two Phase ◽  
Solid Ratio ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Solanum Nigrum L ◽  
Aqueous Two Phase Extraction

Background: Solasodine is a major bioactive ingredient in Solanum nigrum L. that has strong pharmacological characteristics. Therefore, the development of a simple and effective extraction method for obtaining solasodine is highly important. This study aims to provide a rapid and effective method for extracting solasodine from Solanum nigrum L. by microwave-assisted aqueous two-phase extraction (MAATPE). Methods: First, the high-performance liquid chromatography (HPLC) conditions were established for the detection of solasodine. Then, the aqueous two-phase system (ATPS) compositions were examined. On the basis of the results of single-factor experiments, for a better yield, response surface methodology (RSM) was used to optimize influential factors including the extraction temperature, extraction time and liquid-to-solid ratio. Results: The maximum extraction yield of 7.11 ± 0.08 mg/g was obtained at 44 °C, an extraction time of 15 min, and a liquid-to-solid ratio of 42:1 mL/g in the ATPS consisting of EtOH solvent, (NH4)2SO4, and water (28:16:56, w/w/w). The extraction yield of the alkaloid obtained using this method was markedly higher than those of microwave-assisted extraction (MAE) and ultrasonic-assisted extraction (UAE). Conclusions: In this work, solasodine was extracted by MAATPE for the first time and a high yield was obtained. MAATPE is a simple, rapid, and green technique for extraction from medical plants. Thus, the present study will enable the development of a feasible extraction method of active alkaloids from Solanum nigrum L.

scholarly journals Ultrasonic Treatment Increases Extraction Rate of Common Bean (Phaseolus vulgaris L.) Antioxidants

Antioxidants ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 83 ◽  
Author(s):  
Qiong-Qiong Yang ◽  
Ren-You Gan ◽  
Ying-Ying Ge ◽  
Dan Zhang ◽  
and Harold Corke
Keyword(s):  
Common Bean ◽  
Ultrasonic Treatment ◽  
High Performance ◽  
Extraction Rate ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Infrared Spectrophotometer ◽  
Conventional Solvent Extraction ◽  
Ft Ir

The feasibility of improving the extraction rate of common bean antioxidants by ultrasonic treatment was investigated. Scanning electron microscopy (SEM) and spectrum Fourier transform infrared spectrophotometer (FT-IR) analysis revealed that ultrasonic treatment substantially altered the cellular structure of common bean seed, resulting in increased surface area, eroded cell walls, and greater exposure of cellulose and hemicellulose. The highest antioxidant activity was obtained at optimal extraction conditions (68 min, 55% acetone, 36:1 liquid to solid ratio, 30 ℃, and 480 W) which were optimized by response surface methodology. In terms of the extraction rate of common bean antioxidants, ultrasound-assisted extraction (UAE) exhibits about seven-fold higher extraction efficiency than conventional solvent extraction (CSE). In addition, 10 phenolic compounds in the common bean extracts were detected and quantified by high performance liquid chromatography (HPLC), including protocatechuic acid, catechin, chlorogenic acid, epicatechin, ferulic acid, coumarin, rutin, myricetin, cinnamic acid, and genistein. In summary, ultrasonic treatment is an ideal candidate methodology for improving the extraction rate of common bean antioxidants.

Multi-residue determination of micropollutants in Nigerian fish from Lagos lagoon using ultrasound assisted extraction, solid phase extraction and ultra-high-performance liquid chromatography tandem mass spectrometry

2020 ◽  
Vol 12 (16) ◽  
pp. 2114-2122
Author(s):  
Idera Fabunmi ◽  
Natalie Sims ◽  
Kathryn Proctor ◽  
Aderonke Oyeyiola ◽  
Temilola Oluseyi ◽  
...  
Keyword(s):  
High Performance ◽  
Solid Phase ◽  
Ultrasound Assisted Extraction ◽  
Residue Determination ◽  
Chromatography Tandem Mass Spectrometry ◽  
Assisted Extraction ◽  
Liquid Chromatography Tandem Mass ◽  
Lagos Lagoon ◽  
First Time

This reports for the first time a simple and robust approach in determining pharmaceuticals in different fish species in Nigeria.

scholarly journals HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FOR DETERMINATION OF METOPROLOL ENANTIOMERS IN PHARMACEUTICAL FORMULATIONS

2014 ◽  
Vol 10 (4) ◽  
pp. 2654-2660
Author(s):  
Sylwia Magiera ◽  
PhD Weronika Adolf ◽  
Prof. Irena Baranowska
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Direct Determination ◽  
Pharmaceutical Formulations ◽  
Ultrasound Assisted Extraction ◽  
Fluorescence Detector ◽  
Extraction Solvent ◽  
Assisted Extraction

A novel high performance liquid chromatography with fluorescence detector (HPLC-FL) method for determination of metoprolol enantiomers in a pharmaceutical formulation was developed and validated. This study investigated the ultrasound-assisted extraction (UAE) of metoprolol from commercial tablets using methanol as extraction solvent. The separation of enantiomers was successfully carried out with a CHIRALCEL OD-RH column, under isocratic elution mode using 0.05% trifluoroacetic acid and 0.05% diethylamine in water and acetonitrile (80:20, v/v).The chromatographic method was validated in terms of precision, accuracy, detection and quantification limits, linearity and recovery. Calibration curves were linear in the investigated range with correlation coefficient better than 0.9931 while the limit of quantifications was 0.19 µg mL-1 for S(–)-MET and 0.23 µg mL-1 for R(+)-MET. The mean recovery of S(–)-MET and R(+)-MET from tablets were found to be 78.8% and 92.1%, respectively.This method for the direct determination and quantification of metoprolol enantiomers in pharmaceutical formulations is suitable for routine analyses in quality control laboratories and was applied to evaluate for the first time, the presence and the quantities of cited analytes in commercially available formulation. 

Ultrasound-assisted extraction coupled with SPE-HPLC-DAD for the determination of three bioactive phenylpropanoids from Radix Isatidis

2014 ◽  
Vol 6 (18) ◽  
pp. 7547-7553 ◽  
Author(s):  
Ping Xiao ◽  
Jianwei Chen ◽  
Xiang Li ◽  
Yayun Chen
Keyword(s):  
High Performance ◽  
Reversed Phase ◽  
Diode Array ◽  
Ultrasound Assisted Extraction ◽  
Radix Isatidis ◽  
Rp Hplc ◽  
Assisted Extraction ◽  
Array Detection ◽  
Ultrasound Assisted

This study demonstrated a reversed-phase high performance liquid chromatography (RP-HPLC) with photo-diode array detection (DAD) method for simultaneous determination of three phenylpropanoids including syringin, clemastanin B and indigoticoside A in Radix Isatidis.

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