Antioxidant Activity of Pastinaca sativa L. ssp. sylvestris [Mill.] Rouy and Camus Essential Oil

In the last decade, there has been growing interest in the food industry in replacing synthetic chemicals with natural products with bioactive properties. This study’s aims were to determine the chemical composition and the antioxidant properties of the essential oil of Pastianica sylvestris. The essential oil was isolated with a yield of 0.41% (w/v) by steam distillation from the dried seeds and subsequently analysed by GC-MS. Octyl acetate (78.49%) and octyl hexanoate (6.68%) were the main components. The essential oil exhibited an excellent activity for the inhibition of primary and secondary oxidation products for cold-pressed sunflower oil comparable with butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT), which were evaluated using peroxide and thiobarbituric acid values. The antioxidant activity of the essential oil was additionally validated using DPPH radical scavenging (0.0016 ± 0.0885 mg/mL), and β-carotene-linoleic acid bleaching assays. Also, the amounts of total phenol components (0.0053 ± 0.0023 mg GAE/g) were determined.

Download Full-text

Composition and Antioxidant Activity of Essential Oils of Lippia Origanoides H.B.K. grown in Colombia

Natural Product Communications ◽

10.1177/1934578x0800300417 ◽

2008 ◽

Vol 3 (4) ◽

pp. 1934578X0800300 ◽

Cited By ~ 5

Author(s):

Elena Stashenko ◽

Carlos Ruiz ◽

Amner Muñoz ◽

Martha Castañeda ◽

Jairo Martínez

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

The Other ◽

Butylated Hydroxytoluene ◽

Scavenging Activity ◽

Compound Identification ◽

Microwave Assisted ◽

Main Components

Microwave-assisted hydrodistillation, simultaneous distillation-solvent extraction, and supercritical fluid extraction, were used to isolate secondary metabolites from two Lippia origanoides chemotypes growing wild in Colombia. Compound identification was based on chromatographic and spectroscopic criteria. The main components identified in the essential oil of one chemotype were carvacrol (44.4 – 51.8%) and p-cymene (8.8 – 10.1%). Those of the other chemotype were p-cymene (11.3 – 15.7%) and 1,8-cineole (6.8 – 10.9%). The last one constitutes a new chemotype, characterized by its low thymol and carvacrol contents and very low antioxidant activity. The radical scavenging activity of the essential oil of the first chemotype was higher than that of butylated hydroxytoluene (BHT) and similar to that of α-tocopherol.

Download Full-text

Chemical Analysis and Antioxidant Activity of the Essential Oils of Three Piperaceae Species Growing in the Central Region of Cuba

Natural Product Communications ◽

10.1177/1934578x1300800935 ◽

2013 ◽

Vol 8 (9) ◽

pp. 1934578X1300800 ◽

Cited By ~ 3

Author(s):

Elisa Jorge Rodríguez ◽

Yanelis Saucedo-Hernández ◽

Yvan Vander Heyden ◽

Ernesto F. Simó-Alfonso ◽

Guillermo Ramis-Ramos ◽

...

Keyword(s):

Antioxidant Activity ◽

Essential Oils ◽

Central Region ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Thiobarbituric Acid ◽

Gas Chromatography Mass Spectrometry ◽

Radical Scavenging Ability ◽

Main Components

The present study describes the phytochemical profile and antioxidant activity of the essential oils of three Piperaceae species collected in the central region of Cuba. The essential oils of Piper aduncum, P. auritum and P. umbellatum leaves, obtained by hydrodistillation, were analyzed by gas chromatography-mass spectrometry. The main components of P. aduncum oil were piperitone (34%), camphor (17.1%), camphene (10.9%), 1,8-cineol (8.7%) and viridiflorol (7.4%), whereas that of P. auritum and P. umbellatum was safrole (71.8 and 26.4%, respectively). The antioxidant properties of the essential oils were also evaluated using several assays for radical scavenging ability (DPPH test and reducing power) and inhibition of lipid oxidation (ferric thiocyanate method and evaluation against Cucurbita seed oil by peroxide, thiobarbituric acid and p-anisidine methods). P. auritum showed the strongest antioxidant activity among the Piper species investigated, but lower than those of butylated hydroxyanisol and propyl gallate.

Download Full-text

Chemical Composition, Antioxidant Properties and Antimicrobial Activity of the Essential Oil of Murraya Paniculata Leaves from the Mountains of Central Cuba

Natural Product Communications ◽

10.1177/1934578x1200701129 ◽

2012 ◽

Vol 7 (11) ◽

pp. 1934578X1200701 ◽

Cited By ~ 5

Author(s):

Elisa Jorge Rodríguez ◽

Guillermo Ramis-Ramos ◽

Yvan Vander Heyden ◽

Ernesto F. Simó-Alfonso ◽

María Jesús Lerma-García ◽

...

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Seed Oil ◽

Antioxidant Properties ◽

Thiobarbituric Acid ◽

Butylated Hydroxytoluene ◽

Gas Chromatography Mass Spectrometry ◽

Butylated Hydroxyanisole ◽

Inhibitory Effects ◽

Murraya Paniculata

The essential oil of Murraya paniculata L leaves from the mountains of the Central Region of Cuba, obtained by hydrodistillation, was analyzed by gas chromatography-mass spectrometry. Eighteen compounds, accounting for 95.1% of the oil were identified. The major component was β-caryophyllene (ca. 30%). The antioxidant activity of essential oil was evaluated against Cucurbita seed oil by peroxide, thiobarbituric acid and p-anisidine methods. The essential oil showed stronger antioxidant activity than that of butylated hydroxyanisole and butylated hydroxytoluene, but lower than that of propyl gallate. Moreover, this antioxidant activity was supported by the complementary antioxidant assay in the linoleic acid system and 2, 2′-diphenyl-1-picrylhydrazyl. The essential oil also showed good to moderate inhibitory effects against Klebsiella pneumoniae and Bacillus subtilis.

Download Full-text

Chemical Composition, Antimicrobial and Antioxidant Properties of the Volatile Oil and Methanol Extract of Xenophyllum Poposum

Natural Product Communications ◽

10.1177/1934578x1200701230 ◽

2012 ◽

Vol 7 (12) ◽

pp. 1934578X1200701 ◽

Cited By ~ 4

Author(s):

Ana M. González ◽

María I Tracanna ◽

Sara M. Amani ◽

Carola Schuff ◽

Miguel J. Poch ◽

...

Keyword(s):

Essential Oil ◽

Methanol Extract ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Pathogenic Fungi ◽

Volatile Oil ◽

Antibacterial Activities ◽

Butylated Hydroxytoluene ◽

Fungal Strains ◽

Main Components

The essential oil and methanol extract of northwestern Argentina medicinal plant Xenophyllum poposum, collected in Catamarca province, were investigated. GC and GC-MS analyses of the essential oil identified 56 compounds accounting for 92.9%. The main components of the oil were δ-cadinene (16.5%), 6-hydroxytremetone (14.7%), epi-α-cadinol (12.0%), α-cadinol (8.8%), γ-cadinene (7.5%), 1- epi-cubenol (4.2%) and α-muurolene (3.0%). The essential oil exhibited antibacterial activities against five pathogenic strains as well as antifungal activities against two pathogenic fungi. The methanol extract showed antibacterial activity against two strains of Staphylococcus aureus and two pathogenic fungal strains. The main components isolated from the methanol extract were the antifungal 4-hydroxy-3-(isopenten-2-yl)-acetophenone, 6-hydroxytremetone, and tremetone. 6-Hydroxytremetone showed activity against all the fungal strains and one of the S. aureus strains assayed. Antioxidant and radical-scavenging properties of the methanol extract and essential oil were determined using the 2,2′-diphenyl-1-picrylhydrazyl assay and β-carotene bleaching (BCB) test. The methanol extract and the essential oil showed, respectively, moderate and weak antioxidant activity when compared to butylated hydroxytoluene.

Download Full-text

Chemical Composition, In Vitro and In Silico Antioxidant Potential of Melissa officinalis subsp. officinalis Essential Oil

Antioxidants ◽

10.3390/antiox10071081 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1081

Author(s):

Matilda Rădulescu ◽

Călin Jianu ◽

Alexandra Teodora Lukinich-Gruia ◽

Marius Mioc ◽

Alexandra Mioc ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

In Silico ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Inhibitory Effect ◽

Melissa Officinalis ◽

Β Carotene

The investigation aimed to study the in vitro and in silico antioxidant properties of Melissa officinalis subsp. officinalis essential oil (MOEO). The chemical composition of MOEO was determined using GC–MS analysis. Among 36 compounds identified in MOEO, the main were beta-cubebene (27.66%), beta-caryophyllene (27.41%), alpha-cadinene (4.72%), caryophyllene oxide (4.09%), and alpha-cadinol (4.07%), respectively. In vitro antioxidant properties of MOEO have been studied in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging, and inhibition of β-carotene bleaching assays. The half-maximal inhibitory concentration (IC50) for the radical scavenging abilities of ABTS and DPPH were 1.225 ± 0.011 μg/mL and 14.015 ± 0.027 μg/mL, respectively, demonstrating good antioxidant activity. Moreover, MOEO exhibited a strong inhibitory effect (94.031 ± 0.082%) in the β-carotene bleaching assay by neutralizing hydroperoxides, responsible for the oxidation of highly unsaturated β-carotene. Furthermore, molecular docking showed that the MOEO components could exert an in vitro antioxidant activity through xanthine oxidoreductase inhibition. The most active structures are minor MOEO components (approximately 6%), among which the highest affinity for the target protein belongs to carvacrol.

Download Full-text

Essential Oil Composition and Antioxidant Activity of Marrubium vulgare L. Growing Wild in Eastern Algeria

International Letters of Chemistry Physics and Astronomy ◽

10.18052/www.scipress.com/ilcpa.14.17 ◽

2013 ◽

Vol 14 ◽

pp. 17-24 ◽

Cited By ~ 4

Author(s):

Abderazak Abadi ◽

Aicha Hassani

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Essential Oil Composition ◽

Oil Composition ◽

Aerial Parts ◽

Marrubium Vulgare ◽

Eastern Algeria

In previous work [1], the essential oil of the aerial parts of Marrubium vulgare L. obtained by hydrodistillation was analysed by gas chromatography coupled to mass spectrometry (GC-MS) in order to determine their chemical composition. Fifty (50) components in the oil of M. vulgare were identified. The results demonstrated that the major components of the essential oil were: 4,8,12,16-Tetramethyl heptadecan-4-olid (16.97 %), Germacrene D-4-ol (9.61 %), α- pinéne (9.37 %), Phytol (4.87 %), Dehydro-sabina ketone (4.12 %), Piperitone (3.27 %), δ-Cadinene (3.13 %), 1-Octen-3-ol (2.35 %) and Benzaldehyde (2.31 %). In this study, the antioxidant properties of essential oil were examined. The results showed that this oil can be considered an effective source of antioxidants of natural origin. This is the first report on chemical composition of M. vulgare essential oil cultivated in Algeria and the original study on the antioxidant activity of M. vulgare essential oil. The antioxidant activity was investigated with one method: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method.

Download Full-text

Chemical Constituents, Antioxidative and Antimicrobial Activities of Essential Oil and Oleoresin of Tailed Pepper (Piper Cubeba L)

International Journal of Food Engineering ◽

10.2202/1556-3758.1154 ◽

2007 ◽

Vol 3 (6) ◽

Cited By ~ 13

Author(s):

Gurdip Singh ◽

Palanisamy Marimuthu ◽

Carola S. de Heluani ◽

Cesar A. N. Catalan

Keyword(s):

Essential Oil ◽

Chemical Constituents ◽

Radical Scavenging ◽

Reducing Power ◽

Chemical Characterization ◽

Thiobarbituric Acid ◽

Antimicrobial Activities ◽

Butylated Hydroxytoluene ◽

Mustard Oil ◽

Activity Measurements

The antimicrobial and antioxidant potentials of hydrodistilled essential oil and oleoresin (obtained using acetone as a solvent) of tailed pepper were carried out by different techniques. The results obtained from antioxidant activity measurements of essential oil and oleoresin against mustard oil were measured for duration of 28 days in terms of peroxide, thiobarbituric acid, total carbonyl and p-anisidine values. The results obtained from butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) was similar to both the oleoresin and essential oil. In addition, the inhibitory action in linoleic acid system was studied by monitoring accumulation of peroxide concentration. The radical scavenging capacity of both essential oil and oleoresin on 2, 2'-diphenyl-1-picrylhydrazyl (DPPH) radical were (71.2%) and (69.77%) respectively at 25 µL/mL. It was relatively lower in comparison with synthetic antioxidants (BHA-96.41%; BHT- 95.91%). The results obtained from reducing power, chelating effect and hydroxyl radical scavenging effect was also supported the antioxidant of essential oil and oleoresin. The tailed pepper essential oil and oleoresin showed 100% mycelial zone inhibition against Penicillium viridicatum at 3000 and 2000 ppm respectively in the poison food method. It is interesting to note that the essential oil revealed 100% clear zone inhibition against Aspergillus flavus at all tested concentrations. The chemical characterization of tailed pepper essential oil by GC and GC-MS resulted in the identification of 44 components accounting for 97.8% of the oil.

Download Full-text

In-vitro, in-vivo, and in-silico assessment of radical scavenging and cytotoxic activities of Oliveria decumbens essential oil and its main components

Scientific Reports ◽

10.1038/s41598-021-93535-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Tahereh Jamali ◽

Gholamreza Kavoosi ◽

Yousef Jamali ◽

Saeed Mortezazadeh ◽

Susan K. Ardestani

Keyword(s):

Nitric Oxide ◽

Flow Cytometry ◽

Essential Oil ◽

Cancer Cells ◽

In Silico ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Main Components

AbstractWe aimed to explore and compare new insights on the pharmacological potential of Oliveria decumbence essential oil (OEO) and its main components highlighting their antioxidant activity in-vitro, in-vivo, and in-silico and also cytotoxic effects of OEO against A549 lung cancer cells. At first, based on GC–MS analysis, thymol, carvacrol, p-cymene, and γ-terpinene were introduced as basic ingredients of OEO and their in-vitro antioxidant capacity was considered by standard methods. Collectively, OEO exhibited strong antioxidant properties even more than its components. In LPS-stimulated macrophages treated with OEO, the reduction of ROS (Reactive-oxygen-species) and NO (nitric-oxide) and down-regulation of iNOS (inducible nitric-oxide-synthase) and NOX (NADPH-oxidase) mRNA expression was observed and compared with that of OEO components. According to the results, OEO, thymol, and carvacrol exhibited the highest radical scavenging potency compared to p-cymene, and γ-terpinene. In-silico Molecular-Docking and Molecular-Dynamics simulation indicated that thymol and carvacrol but no p-cymene and γ-terpinene may establish coordinative bonds in iNOS active site and thereby inhibit iNOS. However, they did not show any evidence for NOX inhibition. In the following, MTT assay showed that OEO induces cytotoxicity in A549 cancer cells despite having a limited effect on L929 normal cells. Apoptotic death and its dependence on caspase-3 activity and Bax/Bcl2 ratio in OEO-treated cells were established by fluorescence microscopy, flow cytometry, colorimetric assay, and western blot analysis. Additionally, flow cytometry studies demonstrated increased levels of ROS in OEO-treated cells. Therefore, OEO, despite showing antioxidant properties, induces apoptosis in cancer cells by increasing ROS levels. Collectively, our results provided new insight into the usage of OEO and main components, thymol, and carvacrol, into the development of novel antioxidant and anti-cancer agents.

Download Full-text

Chemical composition and antioxidant activity of essential oil of Cymbopogon flexuosus

Journal of Applied and Natural Science ◽

10.31018/jans.v12i1.2207 ◽

2020 ◽

Vol 12 (1) ◽

pp. 25-29

Author(s):

Anju Bhatnagar

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Racemic Mixture ◽

Oil Composition ◽

Cymbopogon Flexuosus ◽

Ic50 Value

Cymbopogon species from the Poaceae family are widely distributed in the Himalayan region of India and commonly used as flavors, fragrances, cosmetics, and pharmaceuticals. It is known to contain compound citral, which give the lemon scent to many of the plants of the cymbopogon genera. The essential oil of Cymbopogon flexuosus has high polyphenolic content which is responsible for antioxidant properties. Beside citral is also used for the synthesis of vitamin B and Ionones. The bioactive potential of Lemongrass and constituent are rapidly increasing which is reflected from growing number of reports being published. The present study was to know the chemical composition and in vitro antioxidant activity of essential oil of C. flexuosus from Uttarakhand. The essential oils of Cymbopogon collected in the region of Uttarakhand were obtained by hydrodistillation of the leaves and analyzed for chemical composition by GC/MS. The antioxidant activity of essential oils at different concentrations was determined against DPPH radical activity and vitamin C as the standard antioxidant compound. The IC50 value and percentage of DPPH inhibition were recorded. Twenty-five compounds were identified in essential oil extracted from leaves representing 93.15% of the oil composition. The yield of essential oil of Cymbopogon was 0.6 + 0.1 %and the major compound in the essential oil was citral (a racemic mixture of two isoforms geranial and nearl) followed by heptenone(1.98%) , linalool(1.65%), geraniol (1.47%), ?-caryophyllene (1.14% ) , limonene (0.92%), nearl acetate (0.82%), citronellal(0.44 %) and citronellol (0.22%). Radical scavenging capacity (Inhibition, %) of the C. flexuosus essential oil was high (78.19+1.11) at the concentration level of 150 ?g/ml and IC50 value of the essential oil was 43.67?g/ml. The data of this study encourages to consider the essential oil of C. flexuosus as a source of bioactive compounds which may add great industrial value to this crop.

Download Full-text

In vivo and in vitro antioxidant effects of three Veronica species

Open Life Sciences ◽

10.2478/s11535-012-0041-4 ◽

2012 ◽

Vol 7 (3) ◽

pp. 559-568 ◽

Cited By ~ 4

Author(s):

Jelena Živković ◽

Tatjana Ćebović ◽

Zoran Maksimović

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Thiobarbituric Acid ◽

Butylated Hydroxytoluene ◽

Antioxidant Systems ◽

Antioxidant Power ◽

Antioxidant Effects

AbstractThe aim of the present study was to examine the antioxidant activity of three Veronica species (Plantaginaceae). The antioxidant potential of various extracts obtained from aerial flowering parts was evaluated by DPPH-free (1,1-diphenyl-2-picrylhydrazyl-free) radical scavenging activity and ferric-reducing antioxidant power assays. Considerable antioxidant activity was observed in the plant samples (FRAP values ranged from 0.97 to 4.85 mmol Fe2+/g, and DPPH IC50 values from 12.58 to 66.34 µg/ml); however, these levels were lower than the activity of the control compound butylated hydroxytoluene (BHT) (FRAP: 10.58 mmol Fe2+/g; DPPH IC50: 9.57 µg/ml). Also, the in vivo antioxidant effects were evaluated in several hepatic antioxidant systems in rats (activities of glutathione peroxidase, glutathione reductase, peroxidase, catalase, xanthine oxidase, glutathione content and level of thiobarbituric acid reactive substances) after treatment with different Veronica extracts, or in combination with carbon tetrachloride (CCl4). Pretreatment with 100 mg/kg b.w. of Veronica extracts inhibited CCl4-induced liver injury by decreasing TBA-RS level, increasing GSH content, and bringing the activities of CAT and Px to control levels. The present study suggests that the extracts analyzed could protect the liver cells from CCl4-induced liver damage by their antioxidative effect on hepatocytes.

Download Full-text