scholarly journals Enhanced Degradation of Naproxen by Immobilization of Bacillus thuringiensis B1(2015b) on Loofah Sponge

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 872 ◽  
Author(s):  
Anna Dzionek ◽  
Danuta Wojcieszyńska ◽  
Małgorzata Adamczyk-Habrajska ◽  
Urszula Guzik
Keyword(s):  
Bacillus Thuringiensis ◽  
System Analysis ◽  
Treatment Plant ◽  
Trickling Filter ◽  
Significant Drop ◽  
Trickling Filters ◽  
Degrading Bacterium ◽  
Loofah Sponge ◽  
Immobilised Cells ◽  
Bacterium Bacillus

The naproxen-degrading bacterium Bacillus thuringiensis B1(2015b) was immobilised onto loofah sponge and introduced into lab-scale trickling filters. The trickling filters constructed for this study additionally contained stabilised microflora from a functioning wastewater treatment plant to assess the behavior of introduced immobilized biocatalyst in a fully functioning bioremediation system. The immobilised cells degraded naproxen (1 mg/L) faster in the presence of autochthonous microflora than in a monoculture trickling filter. There was also abundant colonization of the loofah sponges by the microorganisms from the system. Analysis of the influence of an acute, short-term naproxen exposure on the indigenous community revealed a significant drop in its diversity and qualitative composition. Bioaugmentation was also not neutral to the microflora. Introducing a new microorganism and increasing the removal of the pollutant caused changes in the microbial community structure and species composition. The incorporation of the immobilised B1(2015b) was successful and the introduced strain colonized the basic carrier in the trickling filter after the complete biodegradation of the naproxen. As a result, the bioremediation system could potentially be used to biodegrade naproxen in the future.

Heating up trickling filters to tackle cold weather conditions

2000 ◽  
Vol 41 (1) ◽  
pp. 163-166 ◽  
Author(s):  
W. Gebert ◽  
P.A. Wilderer
Keyword(s):  
Waste Heat ◽  
Treatment Plant ◽  
Weather Conditions ◽  
Pilot Scale ◽  
Cold Weather ◽  
Filling Material ◽  
Trickling Filter ◽  
Biofilm Thickness ◽  
Trickling Filters ◽  
Biofilm Support

The investigated effects of heating the filling material in trickling filters were carried out at the Ingolstadt wastewater treatment plant, Germany. Two pilot scale trickling filters were set up. Heat exchanger pipings were embedded in the filter media of one of these trickling filters, and the temperature in the trickling filter was raised. The other trickling filter was operated under normal temperature conditions, and was used as a control. The results clearly demonstrate that the performance of trickling filters cannot be constantly improved by heating the biofilm support media. A sustained increase of the metabolic rates did not occur. The decrease of the solubility of oxgen in water and mass transfer limitations caused by an increase of the biofilm thickness are the main reasons for that. Thus, the heating of trickling filters (e.g. by waste heat utilization) in order to increase the capacity of trickling filters under cold weather conditions cannot be recommended.

Maximizing trickling filter nitrification rates through biofilm control: research review and full scale application

1997 ◽  
Vol 36 (1) ◽  
pp. 255-262 ◽  
Author(s):  
Denny S. Parker ◽  
Tom Jacobs ◽  
Erich Bower ◽  
Dennis W. Stowe ◽  
Greg Farmer
Keyword(s):  
Control Strategies ◽  
Treatment Plant ◽  
Ammonia Nitrogen ◽  
Full Scale ◽  
Research Review ◽  
Trickling Filter ◽  
Trickling Filters ◽  
The Past ◽  
Biofilm Control ◽  
Control Research

Tertiary nitrifying trickling filters (NTFs) at the Littleton/Englewood wastewater treatment plant provide for nitrification to meet seasonally varying effluent requirements for ammonia nitrogen. Operation of the full-scale facilities during the past two years demonstrates highly efficient oxidation of ammonia and the effectiveness of biofilm control strategies. A decline in nitrification performance caused by predators was successfully corrected by the use of a special alkaline backwash feature which controlled the level of larval development within the NTFs.

Extension of a Large Trickling Filter Plant for Combined Nitrogen and Phosphorus Removal (Ejby Mølle Wastewater Treatment Plant, Odense, Denmark)

1991 ◽  
Vol 23 (4-6) ◽  
pp. 791-800 ◽  
Author(s):  
Knud Christensen
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Trickling Filter ◽  
Nitrogen And Phosphorus ◽  
Nitrogen And Phosphorus Removal ◽  
Trickling Filters ◽  
Future Extension ◽  
First Results ◽  
P Removal

Until 1985, one of the wastewater treatment plants in Odense, Denmark, was a traditional trickling filter plant designed for approx. 275,000 PE. To meet rigorous standards for N and P removal, the plant was extended in 1986-1988, and the extended plant was put into operation in April, 1989. Now, standards have been changed again and are : N < 8 mg/l and P < 0.5 mg/l. These standards must be complied, with before 1992. The establishing of design criteria based on statistical methods is presented. On the basis thereof, one of various extension alternatives was chosen. This involved keeping the existing primary settling tanks and performing partial nitrification in the existing trickling filters succeeded by a new structure for N and P removal according to the BIO-DENIPHO method. The future extension for contact filtration is mentioned. Basic civil engineering data are described together with design and operating economy. Furthermore, mention is made of the commissioning procedure and the first results.

Rehabilitation and Upgrading of the Beni Suef City Wastewater Treatment Plant

1990 ◽  
Vol 22 (7-8) ◽  
pp. 131-138
Author(s):  
Ahmed Fadel
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Primary Treatment ◽  
High Rate ◽  
Shock Load ◽  
Economic Evaluations ◽  
Activated Sludge Process ◽  
Trickling Filter

Many of Egypt's cities have existing treatment plants under operation that have been constructed before 1970. Almost all of these treatment plants now need rehabilitation and upgrading to extend their services for a longer period. One of these plants is the Beni Suef City Wastewater Treatment Plant. The Beni Suef WWTP was constructed in 1956. It has primary treatment followed by secondary treatment employing intermediate rate trickling filters. The BOD, COD, and SS concentration levels are relatively high. They are approximately 800, 1100, and 600 mg/litre, respectively. The Beni Suef city required the determination of the level of work needed for the rehabilitation and upgrading of the existing 200 l/s plant and to extend its capacity to 440 l/s at year 2000 A description of the existing units, their deficiencies and operation problems, and the required rehabilitation are presented and discussed in this paper. Major problems facing the upgrading were the lack of space for expansion and the shortage of funds. It was, therefore, necessary to study several alternative solutions and methods of treatment. The choice of alternatives was from one of the following schemes: a) changing the filter medium, its mode of operation and increasing the number of units, b) changing the trickling filter to high rate and combining it with the activated sludge process, for operation by one of several possible combinations such as: trickling filter-solids contact, roughing filter-activated sludge, and trickling filter-activated sludge process, c) dividing the flow into two parts, the first part to be treated using the existing system and the second part to be treated by activated sludge process, and d) expanding the existing system by increasing the numbers of the different process units. The selection of the alternative was based on technical, operational and economic evaluations. The different alternatives were compared on the basis of system costs, shock load handling, treatment plant operation and predicted effluent quality. The flow schemes for the alternatives are presented. The methodology of selecting the best alternative is discussed. From the study it was concluded that the first alternative is the most reliable from the point of view of costs, handling shock load, and operation.

Biological removal of algae in an integrated pond system

1995 ◽  
Vol 31 (12) ◽  
pp. 21-31 ◽  
Author(s):  
P. G. J. Meiring ◽  
R. A. Oellermann
Keyword(s):  
Adsorptive Capacity ◽  
Trickling Filter ◽  
Laboratory Findings ◽  
Oxidation Pond ◽  
Trickling Filters ◽  
Biological Removal ◽  
In Series ◽  
The Media ◽  
Oxidation Ponds ◽  
Dark Green

A system of oxidation ponds in series with a biological trickling filter is described. It was known that this arrangement was incapable of reducing effectively the levels of algae present in the pond liquid even though nitrification was effected because of autotrophic conditions prevailing in the trickling filters. This very low trophic level explained the lack of adsorptive capacity present. By shortcircuiting less than 10 percent of the effluent from a fully loaded primary facultative oxidation pond to the trickling filter, the autotrophuc nature or the film in the trickling filter was sufficiently shifted towards a heterotrophic state that had sufficient adsorptive capacity to retain the majority of the algae. It is concluded that the algae, although being absorbed, stay alive on the film and do not contribute significantly to the carbonaceous load on the trickling filter. Further more the algae, although secluded from all sunlight, actually partake in the purification process, producing an effluent which, unlike a normal humus tank effluent, is surprisingly sparkling clear. This significant observation appears to be in line with laboratory findings by others who, when they artificially immobilised certain species of algae and passed water over them, concluded that the algae retained the potential to remove certain compounds from the water. Conglomerates of biologically flocculated dark-green algae are scoured off the film (or sloughed off as part of the film) and, having been photosynthetically inactive for some days, tend not to float, but settle very rapidly. A very significantly aspect of this development is the great potential it has for practical application in developing countries. The algae sloughed off the media are easily thickened and available for ultimate recovery from the water phase without the addition of chemicals.

Dynamic simulation of a low loaded trickling filter for nitrification

1999 ◽  
Vol 39 (4) ◽  
pp. 163-168 ◽  
Author(s):  
K. Seggelke ◽  
F. Obenaus ◽  
K.-H. Rosenwinkel
Keyword(s):  
Dynamic Simulation ◽  
Weather Conditions ◽  
Full Scale ◽  
Trickling Filter ◽  
Examination Period ◽  
Trickling Filters ◽  
Biofilm Model ◽  
Measuring Campaign

For this report, an existing biofilm model was examined in regard to its suitability for the simulation of full scale trickling filter for nitrification. The system was calibrated using the results ascertained in a measuring campaign under dry weather conditions. The verification was done using the results of a second examination period which included spells of stormwater input. It was possible for all periods to satisfactorily illustrate the degradation performance of the simulated trickling filters in regard to dynamics and quantity.

scholarly journals THE USE OF AGENTS BASED ON THE BACTERIUM BACILLUS THURINGIENSIS VAR. ISRAELENSIS FOR MOSQUITO CONTROL

2019 ◽  
Vol 98 (8) ◽  
pp. 893-896
Author(s):  
Svetlana A. Roslavtseva
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Synergistic Effect ◽  
Mosquito Control ◽  
Mosquito Species ◽  
Bacillus Sphaericus ◽  
Development Of Resistance ◽  
Blood Sucking ◽  
Low Concentrations ◽  
Bacterium Bacillus

Mosquito control is necessary to improve the epidemic and, consequently, the sanitary and hygienic situation in human settlements. At the same time, the safest and more environmentally friendly way of controlling is not the fight against adult mosquitoes, but the treatment of reservoirs with microbiological larvicides based on entomopathogenic, aerobic, spore-forming, saprophytic bacteria Bacillus thuringiensis (de Barjac) (Bti). A new serotype of the bacterium B. thuringiensis was found in Israel in the Negev desert. This serotype being more active against larvae of blood-sucking and non-blood-sucking mosquitoes and midges than previously known serotypes, was named israelensis. Bti endotoxin is a typical insecticide with intestinal type of action for different mosquito species. For example, Bti H14 is highly insecticidal to the larvae of Aedes aegypti and Ae. albopictus at very low concentrations. The parasporal body (endotoxin crystal), a crystalline protein consisted of four main polypeptides and two minor polypeptides, possesses of a larvicidal action. Larvicidal activity is associated with a synergistic effect in a combination of four polypeptides. The possibility of development of resistance to products based on Bti and Bacillus sphaericus in populations of mosquitoes (Culicidae) was investigated. The use of domestic microbiological formulations based on Bti («Baktitsid», «Larviol-pasta», and «Antinat») was shown an eradication the larvae of bloodsucking mosquitoes and midges to be possible and rational, since they are not generated resistant populations of mosquitoes. This is confirmed by more than 30 years of the use of such formulations.

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