scholarly journals Identification of Interleukin-8-Reducing Lead Compounds Based on SAR Studies on Dihydrochalcone-Related Compounds in Human Gingival Fibroblasts (HGF-1 cells) In Vitro

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1382
Author(s):  
Katharina Schueller ◽  
Joachim Hans ◽  
Stefanie Pfeiffer ◽  
Jessica Walker ◽  
Jakob P. Ley ◽  
...  
Keyword(s):  
Cell Model ◽  
Periodontal Diseases ◽  
Oral Care ◽  
Interleukin 8 ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Periodontal Inflammation ◽  
Anti Inflammatory ◽  
Related Compounds

Background: In order to identify potential activities against periodontal diseases, eighteen dihydrochalcones and structurally related compounds were tested in an established biological in vitro cell model of periodontal inflammation using human gingival fibroblasts (HGF-1 cells). Methods: Subsequently to co-incubation of HGF-1 cells with a bacterial endotoxin (Porphyromonas gingivalis lipopolysaccharide, pgLPS) and each individual dihydrochalcone in a concentration range of 1 µM to 100 µM, gene expression of interleukin-8 (IL-8) was determined by qPCR and cellular interleukin-8 (IL-8) release by ELISA. Results: Structure–activity analysis based on the dihydrochalcone backbone and various substitution patterns at its aromatic ring revealed moieties 2′,4,4′,6′-tetrahydroxy 3-methoxydihydrochalcone (7) to be the most effective anti-inflammatory compound, reducing the pgLPS-induced IL-8 release concentration between 1 µM and 100 µM up to 94%. In general, a 2,4,6-trihydroxy substitution at the A-ring and concomitant vanilloyl (4-hydroxy-3-methoxy) pattern at the B-ring revealed to be preferable for IL-8 release inhibition. Furthermore, the introduction of an electronegative atom in the A,B-linker chain led to an increased anti-inflammatory activity, shown by the potency of 4-hydroxybenzoic acid N-vanillylamide (13). Conclusions: Our data may be feasible to be used for further lead structure designs for the development of potent anti-inflammatory additives in oral care products.

scholarly journals Effect of bacterial lipopolysaccharide in primary cultures of human gingival fibroblasts

2017 ◽  
Vol 14 (2) ◽  
Author(s):  
Maria Cecilia Verutti ◽  
Octavio González ◽  
John González ◽  
Gloria Moreno
Keyword(s):  
Healthy Donor ◽  
In Vitro Model ◽  
Periodontal Diseases ◽  
Primary Cultures ◽  
Cell Number ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Short Period ◽  
Vitro Model

Summary: Introduction: different factors participate in the pathogenesis ofperiodontal diseases. One factor is the interaction between the fibroblasts and derived productsfrom the microorganisms found in the periodontal environment. Objective: In this work, cultures ofhuman gingival fibroblasts from a healthy donor were used to characterize the in vitro responses tobacterial lipopolysaccharide. Methods: the proliferative response was evaluated using cell count andexpression of CD14 was assessed by flow cytometry. Results: after 24 hours of culture an increase inthe cell number was detected in cultures treated with 1.0 mg/mL LPS, but these differences were notstatistically significant. Human gingival fibroblasts express CD14, but its expression decreases incells cultivated after a short period of time. Nevertheless, lipopolysaccharide helps to recover theexpression of CD14 in fibroblast after 24 hours of culture. Conclusion: preliminary result suggeststhat expression of CD14 on gingival fibroblasts could be modulated by this bacterial derived toxin.The primary culture of human gingival fibroblasts allows the establishment of an in vitro model toevaluate different processes in development of the periodontal diseases. Key words: Gingivalfibroblasts. Lipopolysaccharide. Periodontal disease.

scholarly journals Expression of vitamin D 1α-hydroxylase in human gingival fibroblasts in vivo

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10279
Author(s):  
Kaining Liu ◽  
Bing Han ◽  
Jianxia Hou ◽  
Jianyun Zhang ◽  
Jing Su ◽  
...  
Keyword(s):  
Vitamin D ◽  
Stage Iv ◽  
Human Gingival Fibroblasts ◽  
Periodontal Pocket ◽  
Control Group ◽  
Gingival Fibroblasts ◽  
Connective Tissues ◽  
Periodontal Inflammation

Background Vitamin D 1α-hydroxylase CYP27B1 is the key factor in the vitamin D pathway. Previously, we analyzed the expression of CYP27B1 in human gingival fibroblasts in vitro. In the present study, we analyzed the gingival expression of CYP27B1 in vivo. Methods Forty-two patients with periodontitis Stage IV Grade C and 33 controls were recruited. All patients with periodontitis had unsalvageable teeth and part of the wall of the periodontal pocket was resected and obtained after tooth extraction. All controls needed crown-lengthening surgery, and samples of gingiva resected during surgery were also harvested. All the individuals’ gingivae were used for immunohistochemistry and immunofluorescence. In addition, gingivae from seventeen subjects of the diseased group and twelve subjects of the control group were analyzed by real-time PCR. Results Expression of CYP27B1 was detected both in gingival epithelia and in gingival connective tissues, and the expression in connective tissues colocalized with vimentin, indicating that CYP27B1 protein is expressed in gingival fibroblasts. The expression of CYP27B1 mRNA in gingival connective tissues and the CYP27B1 staining scores in gingival fibroblasts in the diseased group were significantly higher than those in the control group. Conclusions Expression of CYP27B1 in human gingival tissues was detected, not only in the fibroblasts of gingival connective tissues, but also in the gingival epithelial cells, and might be positively correlated with periodontal inflammation.

scholarly journals Cytotoxic and anti-inflammatory effects of chitosan and hemostatic gelatin in oral cell culture

2021 ◽  
Vol 34 (2) ◽  
pp. 98-103
Author(s):  
Jessica Narvaez-Flores ◽  
Gabriela Vilar-Pineda ◽  
Laura Acosta-Torres ◽  
Rene Garcia-Contreras
Keyword(s):  
Cell Viability ◽  
Protein Detection ◽  
Human Gingival Fibroblasts ◽  
Dependent Manner ◽  
Gingival Fibroblasts ◽  
Bioactive Materials ◽  
Regenerative Dentistry ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Chitosan is a biopolymer with bactericidal/bacteriostatic effect, biocompatible and biodegradable. It has been used in tissue engineering to replace tissues partially or completely by releasing bioactive materials or influencing cell growth, usually in regenerative medicine and dentistry. The aim of this study was to evaluate the cytotoxic and anti-inflammatory effect of chitosan alone or with hemostatic gelatin (Spongostand®) in cultures of human pulp cells (HPC), human gingival fibroblasts (HGF) and mouse pre-osteoblasts (MC3T3-E1, ATCC). HPC and HGF were isolated from patients. Cells were subcultured in DMEM. Chitosan was inoculated at different concentrations (0-0.5%) and hemostatic gelatins impregnated with chitosan (0.19%) were placed directly in the presence of cells and incubated for 24 hours. Cell viability was determined by MTT method and mean cytotoxic concentration (CC50) was calculated from the dose-response curve. Anti-inflammatory effect was calculated from the in vitro gingivitis model induced with interleukin 1beta (IL-1β) in HGF and protein detection. The data were subjected to Shapiro-Wilk, Kruskal-Wallis and Mann-Whitney tests. Experiments were performed in triplicate of three independent assays. Cell viability of HPC, HGF and MC3T3-E1 in contact with chitosan decreased significantly (p<0.05). The HPC were the most sensitive (CC50= 0.18%), followed by HGF (CC50=0.18%) and MC3T3-E1 (CC50= 0.19%). The cytotoxicity of gelatins impregnated with chitosan decreased cell viability of HGF and HPC by 11% and 5%, respectively. The proinflammatory effect was reduced significantly in the gingivitis model. To conclude, chitosan induces moderate cytotoxic effects alone or with hemostatic gelatin at 0.19%, in dose-dependent manner, with anti-inflammatory effects on human gingival fibroblasts. The use of chitosan as a biomaterial can be an excellent choice for use in regenerative dentistry.

scholarly journals Evodiae fructus Extract Inhibits Interleukin-1β-Induced MMP-1, MMP-3, and Inflammatory Cytokine Expression by Suppressing the Activation of MAPK and STAT-3 in Human Gingival Fibroblasts In Vitro

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Hyun-Kyung Song ◽  
Eun-Mi Noh ◽  
Jeong-Mi Kim ◽  
Yong-Ouk You ◽  
Kang-Beom Kwon ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Proinflammatory Cytokines ◽  
Oral Care ◽  
Nuclear Factor Κb ◽  
Cytokine Expression ◽  
Human Gingival Fibroblasts ◽  
Interleukin 1Β ◽  
Gingival Fibroblasts ◽  
Tissue Destruction

Periodontitis is a Gram-negative bacterial infectious disease. Numerous inflammatory cytokines, including interleukin-1β (IL-1β), regulate periodontitis pathophysiology and cause periodontal tissue destruction. In human gingival fibroblasts (HGFs), IL-1β stimulates the production of matrix metalloproteinases (MMPs) and proinflammatory cytokines via various mechanisms. Several transcription factors, such as signal transducer and activator of transcription 3 (STAT-3), activator protein 1 (AP-1), and nuclear factor-κB (NF-κB), regulate gene expression. Mitogen-activated protein kinases (MAPKs) regulate these transcription factors. However, the MAPK/STAT-3 activation signal in HGFs is unknown. We investigated the potential inhibitory effects of the extract of Evodiae fructus (EFE), the dried, ripe fruit of Evodia rutaecarpa, on MMP and proinflammatory cytokine expression in IL-1β-stimulated HGFs. EFE inhibited the expression of MMP-1, MMP-3, and proinflammatory cytokines (TNF-α, IL-6, and IL-8) in IL-1β-stimulated HGFs through the inhibition of IL-1β-induced MAPK/STAT-3 activation. Also, these results suggest that the EFE may be a useful for the bioactive material for oral care.

scholarly journals Cellular effects of glycine and trehalose air-polishing powders on human gingival fibroblasts in vitro

2021 ◽  
Author(s):  
Jens Weusmann ◽  
James Deschner ◽  
Jean-Claude Imber ◽  
Anna Damanaki ◽  
Natalia D. P. Leguizamón ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Function ◽  
Nuclear Translocation ◽  
In Vitro Study ◽  
Human Gingival Fibroblasts ◽  
Mrna Levels ◽  
Gingival Fibroblasts ◽  
Air Polishing ◽  
Wide Range

Abstract Objectives Air-polishing has been used in the treatment of periodontitis and gingivitis for years. The introduction of low-abrasive powders has enabled the use of air-polishing devices for subgingival therapy. Within the last decade, a wide range of different low-abrasive powders for subgingival use has been established. In this study, the effects of a glycine powder and a trehalose powder on human gingival fibroblasts (HGF) were investigated. Methods HGF were derived from three systemically and periodontally healthy donors. After 24 h and 48 h of incubation time, mRNA levels, and after 48 h, protein levels of TNFα, IL-8, CCL2, and VEGF were determined. In addition, NF-κB p65 nuclear translocation and in vitro wound healing were assessed. Statistical analysis was performed by ANOVA and post hoc Dunnett’s and Tukey’s tests (p < 0.05). Results Glycine powder significantly increased the expression of proinflammatory genes and showed exploitation of the NF-κB pathway, albeit trehalose powder hardly interfered with cell function and did not trigger the NF-κB pathway. In contrast to trehalose, glycine showed a significant inhibitory effect on the in vitro wound healing rate. Conclusion Subgingivally applicable powders for air-polishing devices can regulate cell viability and proliferation as well as cytokine expression. Our in vitro study suggests that the above powders may influence HGF via direct cell effects. Trehalose appears to be relatively inert compared to glycine powder.

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