scholarly journals Possibility to Biotransform Anthracyclines by Peroxidases Produced by Bjerkandera adusta CCBAS 930 with Reduction of Geno- and Cytotoxicity and Pro-Oxidative Activity

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 462
Author(s):  
Kamila Rybczyńska-Tkaczyk ◽  
Teresa Korniłłowicz-Kowalska ◽  
Konrad A. Szychowski
Keyword(s):  
Biological Treatment ◽  
Vibrio Fischeri ◽  
Human Fibroblasts ◽  
White Rot ◽  
White Rot Fungus ◽  
Lepidium Sativum ◽  
Anthracycline Antibiotics ◽  
Anthraquinone Dyes ◽  
Before And After ◽  
Lepidium Sativum L

The aim of this study was to evaluate the bioremoval mechanism of anthracycline antibiotics by the white-rot fungus B. adusta CCBAS 930. The activity of oxidoreductases and levels of phenolic compounds and free radicals were determined during the biotransformation of anthraquinone antibiotics: daunomycin (DNR) and doxorubicin (DOX) by B. adusta strain CCBAS 930. Moreover, phytotoxicity (Lepidium sativum L.), ecotoxicity (Vibrio fischeri), genotoxicity and cytotoxicity of anthraquinone dyes were evaluated before and after biological treatment. More than 80% and 90% of DNR and DOX were removed by biodegradation (decolorization). Initial solutions of DNR and DOX were characterized by eco-, phyto-, geno- and cytotoxicity. Despite efficient decolorization, secondary metabolites, toxic to bacteria, formed during biotransformation of anthracycline antibiotics in B. adusta CCBAS 930 cultures. DNR and DOX metabolites did not increase reactive oxygen species (ROS) production in human fibroblasts and resazurin reduction. DNR metabolites did not change caspase-3 activity.

scholarly journals Enhanced Efficiency of the Removal of Cytostatic Anthracycline Drugs Using Immobilized Mycelium of Bjerkandera adusta CCBAS 930

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6842
Author(s):  
Kamila Rybczyńska-Tkaczyk
Keyword(s):  
Superoxide Dismutase ◽  
Free Radicals ◽  
Phenolic Compounds ◽  
Glucose Oxidase ◽  
Biological Treatment ◽  
Lepidium Sativum ◽  
Bjerkandera Adusta ◽  
Anthracycline Antibiotics ◽  
Lepidium Sativum L

The aim of this study was to evaluate the bioremoval of anthracycline antibiotics (daunomycin-DNR, doxorubicin–DOX, and mitoxantrone-MTX) by immobilized mycelium of B. adusta CCBAS 930. The activity of oxidoreductases: versatile peroxidases (VP), superoxide dismutase (SOD), catalase (CAT), and glucose oxidase (GOX), and the levels of phenolic compounds (PhC) and free radicals (SOR) were determined during the biotransformation of anthracyclines by B. adusta strain CCBAS 930. Moreover, the phytotoxicity (Lepidium sativum L.), biotoxicity (MARA assay), and genotoxicity of anthracyclines were evaluated after biological treatment. After 120 h, more than 90% of anthracyclines were removed by the immobilized mycelium of B. adusta CCBAS 930. The effective biotransformation of anthracyclines was correlated with detoxification and reduced genotoxicity.

scholarly journals Biodecolorization of anthraquinone dyes using immobilised mycelium of Bjerkandera adusta CCBAS930

2020 ◽  
Vol 171 ◽  
pp. 01013 ◽  
Author(s):  
Kamila Rybczyńska-Tkaczyk ◽  
Teresa Korniłłowicz-Kowalska
Keyword(s):  
Phenolic Compounds ◽  
Lepidium Sativum ◽  
Carminic Acid ◽  
Secondary Products ◽  
Bjerkandera Adusta ◽  
Anthraquinone Dyes ◽  
Before And After ◽  
Acid Blue 129 ◽  
Acid Blue ◽  
Acid Green 25

The aim of this study was to characterize the activity of oxidoreductases during biotransformation of 0.01% anthraquinone dyes: Alizarin Blue Black B (ABBB) and Acid Blue 129 (AB129), Carminic Acid (CA), Remazol Brilliant Blue R (RBBR), Acid Green 25 (AG25) and Poly R-478 by immobilized strain of Bjerkandera adusta CCBAS 930. Phenolic compounds, phytotoxicity (Lepidium sativum L.), biotoxicity were evaluated to determine the toxicity of anthraquinone dyes before and after the treatment with immobilized B. adusta CCBAS 930. More than 60% of CA and AB129 were removed by immobilized B. adusta CCBAS after 7 days. No secondary products toxic to plants and bacteria were formed during immobilized cultures of B. adusta CCBAS 930.

Comparison of Remazol Brillant Blue Removal from Wastewater by Two Different Organisms and Analysis of Metabolites by GC/MS

2014 ◽  
Vol 97 (5) ◽  
pp. 1416-1420 ◽  
Author(s):  
Hatice Ardag Akdogan ◽  
Merve Canpolat
Keyword(s):  
Biological Treatment ◽  
Extracellular Enzymes ◽  
Low Cost ◽  
Environmental Pollutants ◽  
White Rot ◽  
White Rot Fungus ◽  
Synthetic Dyes ◽  
Biological Degradation ◽  
Peroxidase Enzyme ◽  
Textile Industries

Abstract White rot fungus participates in biological degradation of many organic environmental pollutants. Also, white rot fungus contains a variety of extracellular enzymes, and these enzymes are used for biological degradation of organic matter. We investigated the biological treatment of synthetic dyes, at a low cost and in the shortest possible time, that are used especially in the dye and textile industries and are important polluting agents in the wastewater discharged into the environment by these industries. For this purpose, removal of Remazol Brillant Blue by Pleurotus ostreatus and Coprinus plicatilis was studied. This dye was removed 100% (dye concentration, 10.0 mg/L) by both organisms. Laccase and manganese peroxidase enzyme activities were also monitored. There was an attempt to identify metabolites via GC/MS at the end of the decolorization. No detectable metabolite was found.

Bioremediation of a wine distillery wastewater using white rot fungi and the subsequent production of laccase

2007 ◽  
Vol 56 (2) ◽  
pp. 179-186 ◽  
Author(s):  
P.J. Strong ◽  
J.E. Burgess
Keyword(s):  
Biological Treatment ◽  
Oxygen Demand ◽  
White Rot Fungi ◽  
White Rot ◽  
White Rot Fungus ◽  
Laccase Production ◽  
Total Phenolic ◽  
Pycnoporus Cinnabarinus ◽  
Distillery Wastewater

The aim of this work was to ascertain whether a submerged culture of a white rot fungus could be used to treat distillery wastewater, and whether the compounds present in the wastewater would stimulate laccase production. Trametes pubescens MB 89, Ceriporiopsissubvermispora, Pycnoporus cinnabarinus and UD4 were screened for their ability for the bioremediation of a raw, untreated distillery wastewater as well as distillery wastewater that had been pretreated by polyvinylpolypyrrolidone. Suitability of each strain was measured as a function of decreasing the chemical oxygen demand (COD) and total phenolic compounds concentration and the colour of the wastewater, while simultaneously producing laccase in high titres. After screening, T. pubescens MB 89 was used further in flask cultures and attained 79±1.1% COD removal, 80±4.6% total phenols removal, 71±1.6% decrease in colour at an absorbance of 500 nm and increased the pH from 5.3 to near-neutral. Laccase activity in flask cultures peaked at 4,644±228 units/l, while the activity in a 50 l bubble lift reactor peaked at 12,966±71 units/l. Trametes pubescens MB 89 greatly improved the quality of a wastewater known for toxicity towards biological treatment systems, while simultaneously producing an industrially relevant enzyme.

scholarly journals Three-Dimensional X-Ray Imaging and Analysis of Fungi on and in Wood

2009 ◽  
Vol 15 (5) ◽  
pp. 395-402 ◽  
Author(s):  
Jan Van den Bulcke ◽  
Matthieu Boone ◽  
Joris Van Acker ◽  
Luc Van Hoorebeke
Keyword(s):  
Three Dimensional ◽  
Three Dimensions ◽  
White Rot ◽  
White Rot Fungus ◽  
Fungal Mycelium ◽  
X Ray ◽  
Fungal Exposure ◽  
X Ray Imaging ◽  
Before And After ◽  
Fundamental Research

AbstractAs wood is prone to fungal degradation, fundamental research is necessary to increase our knowledge aiming at product improvement. Several imaging modalities are capable of visualizing fungi, but the X-ray equipment presented in this article can envisage fungal mycelium in wood nondestructively in three dimensions with submicron resolution. Four types of wood subjected to the action of the white rot fungusCoriolus versicolor(Linnaeus) Quélet (CTB 863 A) were scanned using an X-ray-based approach. Comparison of wood volumes before and after fungal exposure, segmented manually or semiautomatically, showed the presence of the fungal mass on and in the wood samples and therefore demonstrated the usefulness of computed X-ray tomography for mycological and wood research. Further improvements to the experimental setup are necessary to resolve individual hyphae and enhance segmentation.

scholarly journals Assessment of changes in the phytotoxic properties of oil-contaminated soils in terms of germination and seedlings length of Lepidium sativum L. after electrochemical cleaning

2019 ◽  
Vol 8 (4) ◽  
pp. 103-107
Author(s):  
Nikolay Sergeevich Shulaev ◽  
Valeria Valeryevna Pryanichnikova ◽  
Ramil Rimovich Kadyrov ◽  
Nikolay Alekseevich Bykovskiy ◽  
Raisa Mukhametovna Damineva
Keyword(s):  
Contaminated Soil ◽  
Contaminated Soils ◽  
Electrochemical Treatment ◽  
Lepidium Sativum ◽  
Treatment Technology ◽  
High Responsiveness ◽  
Before And After ◽  
Electrochemical Cleaning ◽  
Lepidium Sativum L ◽  
Properties Of Soil

Different variants of electric treatment technology are used to solve environmental, technological and agro-melioration problems. This paper assesses the change in the properties of contaminated soil during cleaning by passing small amperage through it. The paper contains the results of the study of changes in the toxic properties of soil contaminated with oil and mineralized formation water at oil production after electrochemical treatment. A biotesting technique with Lepidium sativum L. is used for an integrated assessment of the hazardous properties of contaminated soil after electrical treatment. This biological test object has a high responsiveness to the presence of pollutants in soil and water, as well as the ability to rapidly germinate. The main parameters during the study are the percentage of seed germination and the total length of seedlings (underground and aboveground parts). A statistical analysis of the data is carried out. The phytotoxicity of contaminated soil is compared before and after cleaning. The conclusion is drawn about the potential suitability of this method for the full restoration of natural plant communities at the pollution territory, as well as for conducting complex phytoremediation.

scholarly journals Biotechnological Approaches to Facilitate Gold Recovery from Double Refractory Gold Ores

2020 ◽  
Author(s):  
Keiko Sasaki ◽  
Kojo T. Konadu
Keyword(s):  
Solid Phase ◽  
Matter Content ◽  
Gold Recovery ◽  
White Rot ◽  
White Rot Fungus ◽  
Carbonaceous Matter ◽  
Refractory Gold ◽  
Before And After ◽  
Further Development ◽  
Pollutant Gases

Double refractory gold ore (DRGO) not only include ppt levels of gold grains locked in sulfide minerals but also a problematic amount of carbonaceous matter. This causes a significant recovery loss of gold during cyanidation because of the strong affinity of the Au(CN)2 − with the carbonaceous matter. Combustion decreases the carbonaceous matter content, but also emits pollutant gases like CO2, SO2 and As2O3. Therefore, environmentally-friendly solutions have been explored by using biotechnology. Due to the very small amount of the above targets in the ore, it is challenging to show evidential changes in solid-phase before and after the biomineral processing of DRGO. This chapter introduces the mineralogical and chemical changes in the various solid residues produced during a sequential biotreatment, consisting of the liberation of gold from sulfides by an iron-oxidizer and decomposition of carbonaceous matter by lignin-degrading enzymes (lignin peroxidase, manganese peroxidase, laccase) secreted from a white rot-fungus, which successfully improved of gold recovery to over 90%. In addition, further development of biotechnology in the recovery of gold from DRGO is addressed.

Microbial use of podzol Bh fulvic acids

1969 ◽  
Vol 15 (7) ◽  
pp. 677-680 ◽  
Author(s):  
S. P. Mathur
Keyword(s):  
Soluble Fraction ◽  
Trichoderma Viride ◽  
Phenol Oxidase ◽  
Fulvic Acids ◽  
Sole Source ◽  
Iron Complex ◽  
White Rot ◽  
White Rot Fungus ◽  
Before And After

A Trichoderma viride, a Penicillium frequentons, and an Aspergillus fumigatus were isolated from fulvic acid enrichment cultures. These and a white rot fungus, Poria subacida 17780, were tested for their ability to decompose fulvic acids (FA) in liquid cultures. All of the organisms tested were totally inhibited by the presence of FA at 1% concentration. The soil isolates brought about transformation of the FA to humic acids. In 48 h, the Poria subacida used 66% of the FA (0.05%) present as the sole source of carbon in static replacement culture, and up to 45% in 24 days in a static culture. Loss of FA was measured by photometry and confirmed by determination of the carbon content of FA precipitated as iron complex before and after attack. Phenol oxidase of the Poria subacida apparently had no role in degradation of the FA but could darken the color of a well-aerated medium through oxidative transformation of FA products.Preliminary studies with cell-free preparations of the P. subacida indicated that the breakdown is initiated by an enzyme present in particulate fraction of the cells and this activity is extended or facilitated by a factor present in the soluble fraction of the cells. FA-cleaving enzymes(s) may thus be available for use in controlled FA degradation studies aimed at characterizing soil humus.

scholarly journals New Bioactive Fungal Molecules with High Antioxidant and Antimicrobial Capacity Isolated fromCerrena unicolorIdiophasic Cultures

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Magdalena Jaszek ◽  
Monika Osińska-Jaroszuk ◽  
Grzegorz Janusz ◽  
Anna Matuszewska ◽  
Dawid Stefaniuk ◽  
...  
Keyword(s):  
Vibrio Fischeri ◽  
Antioxidant Properties ◽  
Reduction Rate ◽  
Inhibitory Effect ◽  
White Rot ◽  
White Rot Fungus ◽  
Toxicity Tests ◽  
Bacterial Strains ◽  
Scavenging Effect ◽  
Extracellular Laccase

Three bioactive fractions, extracellular laccase (ex-LAC), crude endopolysaccharides (c-EPL), and a low molecular subfraction of secondary metabolites (ex-LMS), were isolated from the idiophasic cultures of the white rot fungusCerrena unicolor. For the first time, we determined the antioxidant properties of these samples by chemiluminometric measurement (a) and assessment of the scavenging effect on ABTS (b) and the DPPH reduction rate (c). The highest reducing capability was found for the ex-LMS fraction: 39–90% for (a), 20–90% for (b), and 10–59% for (c) at the concentration of 6.25–800 µg/mL. The scavenging abilities of theC. unicolorc-EPL were between 36 and 70% for (a), 2 and 60% for (b), and 28 and 32% for (c) at the concentration of 6.25–800 µg/mL. A very high prooxidative potential was observed for the ex-LAC probes. The preliminary toxicity tests were done using the Microtox system and revealed the following percentage of the toxic effect againstVibrio fischeri: 85.37% for c-EPL, 50.67% for ex-LAC, and 99.8% for ex-LMS, respectively. The ex-LAC sample showed the antibacterial activity againstEscherichia coli, c-EPL againstStaphylococcus aureus, and ex-LMS against both bacterial strains, respectively, but the stronger inhibitory effect was exerted onS. aureus.

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