scholarly journals New Bioactive Fungal Molecules with High Antioxidant and Antimicrobial Capacity Isolated fromCerrena unicolorIdiophasic Cultures

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Magdalena Jaszek ◽  
Monika Osińska-Jaroszuk ◽  
Grzegorz Janusz ◽  
Anna Matuszewska ◽  
Dawid Stefaniuk ◽  
...  
Keyword(s):  
Vibrio Fischeri ◽  
Antioxidant Properties ◽  
Reduction Rate ◽  
Inhibitory Effect ◽  
White Rot ◽  
White Rot Fungus ◽  
Toxicity Tests ◽  
Bacterial Strains ◽  
Scavenging Effect ◽  
Extracellular Laccase

Three bioactive fractions, extracellular laccase (ex-LAC), crude endopolysaccharides (c-EPL), and a low molecular subfraction of secondary metabolites (ex-LMS), were isolated from the idiophasic cultures of the white rot fungusCerrena unicolor. For the first time, we determined the antioxidant properties of these samples by chemiluminometric measurement (a) and assessment of the scavenging effect on ABTS (b) and the DPPH reduction rate (c). The highest reducing capability was found for the ex-LMS fraction: 39–90% for (a), 20–90% for (b), and 10–59% for (c) at the concentration of 6.25–800 µg/mL. The scavenging abilities of theC. unicolorc-EPL were between 36 and 70% for (a), 2 and 60% for (b), and 28 and 32% for (c) at the concentration of 6.25–800 µg/mL. A very high prooxidative potential was observed for the ex-LAC probes. The preliminary toxicity tests were done using the Microtox system and revealed the following percentage of the toxic effect againstVibrio fischeri: 85.37% for c-EPL, 50.67% for ex-LAC, and 99.8% for ex-LMS, respectively. The ex-LAC sample showed the antibacterial activity againstEscherichia coli, c-EPL againstStaphylococcus aureus, and ex-LMS against both bacterial strains, respectively, but the stronger inhibitory effect was exerted onS. aureus.

Enhanced Formation of Extracellular Laccase Activity by the White-Rot Fungus Trametes multicolor

2002 ◽  
pp. 229-241 ◽  
Author(s):  
Johann Hess ◽  
Christian Leitner ◽  
Christiane Galhaup ◽  
Klaus D. Kulbe ◽  
Barbara Hinterstoisser ◽  
...  
Keyword(s):  
Laccase Activity ◽  
White Rot ◽  
White Rot Fungus ◽  
Extracellular Laccase ◽  
Trametes Multicolor

scholarly journals Biodegradability of Extractives in Sapwood and Heartwood from Scots Pine by Sapstain and White-Rot Fungi

Holzforschung ◽  
1999 ◽  
Vol 53 (3) ◽  
pp. 247-252 ◽  
Author(s):  
M.J. Martínez-Inigo ◽  
P. Immerzeel ◽  
A. Gutierrez ◽  
J.C. del Río ◽  
R. Sierra-Alvarez
Keyword(s):  
Scots Pine ◽  
White Rot Fungi ◽  
Inhibitory Effect ◽  
Resin Acids ◽  
White Rot ◽  
White Rot Fungus ◽  
Fungal Degradation ◽  
Steryl Esters ◽  
Funalia Trogii ◽  
Total Extractives

SummaryThe fungal degradation of lipophilic extractives in sapwood and heartwood from Scots pine (Pinus sylvestris) was studied. In sapwood, the white rot fungi,Bjerkanderasp. andFunalia trogii, removed higher amounts of extractives than the sapstain strains,Ophiostoma ainoaeandCeratocystis allantospora. Triglycerides, long chain fatty acids, steryl esters and waxes in pine sapwood were almost completely degraded by all the fungi. Sterols and resin acids were also extensively degraded by the white rot strains; however, these components were not or only poorly removed by the sapstain fungi. The removal of total extractives by all the fungal strains was higher in sapwood as compared to heartwood. The highly concentrated extractive fraction in pine heartwood mainly consists of resin acids. As observed in sapwood, sapstain were also poorly effective in the degradation of the resin acids present in heartwood. The fungal degradation of heartwood extractives was not only limited by the degradative ability of the various test microorganisms, but also by the inhibitory effect exerted by the extractive fraction. The white rot fungusF. trogiiwas particularly inhibited on heartwood.Bjerkanderasp. showed a higher tolerance to toxic extractives and was the most efficient fungus in degrading extractive constituents in both Scots pine heartwood and sapwood. Therefore,Bjerkanderasp. strain BOS55 should be considered as a potential agent for pitch control in pulp and paper manufacture.

scholarly journals Single electron transfer by an extracellular laccase from the white-rot fungus Pleurotus ostreatus

Microbiology ◽  
1995 ◽  
Vol 141 (2) ◽  
pp. 393-398 ◽  
Author(s):  
H.-D. Youn ◽  
K.-J. Kim ◽  
J.-S. Maeng ◽  
Y.-H. Han ◽  
I.-B. Jeong ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Pleurotus Ostreatus ◽  
Single Electron ◽  
White Rot ◽  
White Rot Fungus ◽  
Single Electron Transfer ◽  
Extracellular Laccase

scholarly journals Possibility to Biotransform Anthracyclines by Peroxidases Produced by Bjerkandera adusta CCBAS 930 with Reduction of Geno- and Cytotoxicity and Pro-Oxidative Activity

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 462
Author(s):  
Kamila Rybczyńska-Tkaczyk ◽  
Teresa Korniłłowicz-Kowalska ◽  
Konrad A. Szychowski
Keyword(s):  
Biological Treatment ◽  
Vibrio Fischeri ◽  
Human Fibroblasts ◽  
White Rot ◽  
White Rot Fungus ◽  
Lepidium Sativum ◽  
Anthracycline Antibiotics ◽  
Anthraquinone Dyes ◽  
Before And After ◽  
Lepidium Sativum L

The aim of this study was to evaluate the bioremoval mechanism of anthracycline antibiotics by the white-rot fungus B. adusta CCBAS 930. The activity of oxidoreductases and levels of phenolic compounds and free radicals were determined during the biotransformation of anthraquinone antibiotics: daunomycin (DNR) and doxorubicin (DOX) by B. adusta strain CCBAS 930. Moreover, phytotoxicity (Lepidium sativum L.), ecotoxicity (Vibrio fischeri), genotoxicity and cytotoxicity of anthraquinone dyes were evaluated before and after biological treatment. More than 80% and 90% of DNR and DOX were removed by biodegradation (decolorization). Initial solutions of DNR and DOX were characterized by eco-, phyto-, geno- and cytotoxicity. Despite efficient decolorization, secondary metabolites, toxic to bacteria, formed during biotransformation of anthracycline antibiotics in B. adusta CCBAS 930 cultures. DNR and DOX metabolites did not increase reactive oxygen species (ROS) production in human fibroblasts and resazurin reduction. DNR metabolites did not change caspase-3 activity.

scholarly journals Characterization of a novel laccase purified from the fungus Hohenbuehelia serotina and its decolourisation of dyes.

2015 ◽  
Vol 63 (2) ◽  
Author(s):  
Yingyin Xu ◽  
Yuxiao Lu ◽  
Rui Zhang ◽  
Hexiang Wang ◽  
Qinghong Liu
Keyword(s):  
Molecular Weight ◽  
Enzyme Activity ◽  
Enzymatic Activity ◽  
Inhibitory Effect ◽  
Single Band ◽  
White Rot ◽  
White Rot Fungus ◽  
Sds Page

A novel laccase was purified from the white rot fungus, Hohenbuehelia serotina, to investigate the applications of this laccase in the decoloration of various dyes. SDS-PAGE revealed a single band of this laccase corresponding to a molecular weight of approximately 57.8 kDa. The enzyme showed activity towards several substrates, the most sensitive of which was 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS). The highest enzymatic activity using ABTS as a substrate was observed at pH 6.8 and 30°C. The enzyme activity was found to be significantly enhanced in the presence of Zn(2+) ions and inhibited by Fe(2+) ions. Moreover, SDS and β-mercaptoethanol were inhibitory, and inhibition by L-cysteine was observed while EDTA and DMSO had almost no inhibitory effect. The laccase could effectively decolorize seven different dyes within 30 minutes at 40°C.

scholarly journals Comparatively Investigation of Textile Dye Decolorization by a White Rot Fungus and Various Bacterial Strains

2021 ◽  
Author(s):  
Emre BİRHANLI ◽  
Özfer YEŞİLADA ◽  
Doç. Dr. Ahmet ÇABUK ◽  
Filiz BORAN ◽  
Eray TATLICI
Keyword(s):  
Dye Decolorization ◽  
White Rot ◽  
White Rot Fungus ◽  
Textile Dye ◽  
Bacterial Strains

scholarly journals Inhibitory Effect of N, N-Dimethylhexadecylamine on the Growth of White-Rot Fungus Trametes versicolor (L.) in Wood

Phyton ◽  
2021 ◽  
Vol 90 (1) ◽  
pp. 193-206
Author(s):  
Wilber Montejo-Mayo ◽  
Eduardo D韆s-Rivera ◽  
Mauro Mart韓ez-Pacheco ◽  
Abril Munro-Rojas ◽  
Enrique Ambriz-Parra ◽  
...  
Keyword(s):  
Trametes Versicolor ◽  
Inhibitory Effect ◽  
White Rot ◽  
White Rot Fungus

scholarly journals Exopolysaccharide fromGanoderma applanatumas a Promising Bioactive Compound with Cytostatic and Antibacterial Properties

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Monika Osińska-Jaroszuk ◽  
Magdalena Jaszek ◽  
Magdalena Mizerska-Dudka ◽  
Adriana Błachowicz ◽  
Tomasz Piotr Rejczak ◽  
...  
Keyword(s):  
Vibrio Fischeri ◽  
Cell Damage ◽  
Binding Capacity ◽  
Antibacterial Properties ◽  
Bioactive Compound ◽  
White Rot ◽  
White Rot Fungus ◽  
High Cholesterol ◽  
Bioactive Properties ◽  
Selective Activity

A new exopolysaccharide preparation isolated from stationary cultures of the white rot fungusGanoderma applanatum(GpEPS) was tested in terms of its bioactive properties including its cytotoxic and immunostimulatory effect. The results indicate that the tested GpEPS (at concentrations above 22.85 µg/mL and 228.5 µg/mL) may exhibit selective activity against tumor cells (cell lines SiHa) and stimulate production of TNF-αTHP-1-derived macrophages at the level of 752.17 pg/mL. The GpEPS showed antibacterial properties againstStaphyloccoccus aureusand a toxic effect againstVibrio fischericells (82.8% cell damage). High cholesterol-binding capacity and triglycerides-binding capacity (57.9% and 41.6% after 24 h of incubation with the tested substances, resp.) were also detected for the investigated samples of GpEPS.

scholarly journals Complete oxidation of hydroxymethylfurfural to furandicarboxylic acid by aryl-alcohol oxidase

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Ana Serrano ◽  
Eva Calviño ◽  
Juan Carro ◽  
María I. Sánchez-Ruiz ◽  
F. Javier Cañada ◽  
...  
Keyword(s):  
Plant Biomass ◽  
Reaction Times ◽  
Alcohol Oxidase ◽  
Inhibitory Effect ◽  
Aldehyde Group ◽  
In Situ Monitoring ◽  
White Rot ◽  
White Rot Fungus ◽  
Alternative Mechanism ◽  
Furandicarboxylic Acid

Abstract Background 5-Hydroxymethylfurfural (HMF) is a highly valuable platform chemical that can be obtained from plant biomass carbohydrates. HMF can be oxidized to 2,5-furandicarboxylic acid (FDCA), which is used as a renewable substitute for the petroleum-based terephthalic acid in polymer production. Results Aryl-alcohol oxidase (AAO) from the white-rot fungus Pleurotus eryngii is able to oxidize HMF and its derivative 2,5-diformylfuran (DFF) producing formylfurancarboxylic acid (FFCA) thanks to its activity on benzylic alcohols and hydrated aldehydes. Here, we report the ability of AAO to produce FDCA from FFCA, opening up the possibility of full oxidation of HMF by this model enzyme. During HMF reactions, an inhibitory effect of the H2O2 produced in the first two oxidation steps was found to be the cause of the lack of AAO activity on FFCA. In situ monitoring of the whole reaction by 1H-NMR confirmed the absence of any unstable dead-end products, undetected in the HPLC analyses, that could be responsible for the incomplete conversion. The deleterious effect of H2O2 was confirmed by successful HMF conversion into FDCA when the AAO reaction was carried out in the presence of catalase. On the other hand, no H2O2 formation was detected during the slow FFCA conversion by AAO in the absence of catalase, in contrast to typical oxidase reaction with HMF and DFF, suggesting an alternative mechanism as reported in some reactions of related flavo-oxidases. Moreover, several active-site AAO variants that yield nearly complete conversion in shorter reaction times than the wild-type enzyme have been identified. Conclusions The use of catalase to remove H2O2 from the reaction mixture leads to 99% conversion of HMF into FDCA by AAO and several improved variants, although the mechanism of peroxide inhibition of the AAO action on the aldehyde group of FFCA is not fully understood.

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