scholarly journals Enhanced Efficiency of the Removal of Cytostatic Anthracycline Drugs Using Immobilized Mycelium of Bjerkandera adusta CCBAS 930

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6842
Author(s):  
Kamila Rybczyńska-Tkaczyk
Keyword(s):  
Superoxide Dismutase ◽  
Free Radicals ◽  
Phenolic Compounds ◽  
Glucose Oxidase ◽  
Biological Treatment ◽  
Lepidium Sativum ◽  
Bjerkandera Adusta ◽  
Anthracycline Antibiotics ◽  
Lepidium Sativum L

The aim of this study was to evaluate the bioremoval of anthracycline antibiotics (daunomycin-DNR, doxorubicin–DOX, and mitoxantrone-MTX) by immobilized mycelium of B. adusta CCBAS 930. The activity of oxidoreductases: versatile peroxidases (VP), superoxide dismutase (SOD), catalase (CAT), and glucose oxidase (GOX), and the levels of phenolic compounds (PhC) and free radicals (SOR) were determined during the biotransformation of anthracyclines by B. adusta strain CCBAS 930. Moreover, the phytotoxicity (Lepidium sativum L.), biotoxicity (MARA assay), and genotoxicity of anthracyclines were evaluated after biological treatment. After 120 h, more than 90% of anthracyclines were removed by the immobilized mycelium of B. adusta CCBAS 930. The effective biotransformation of anthracyclines was correlated with detoxification and reduced genotoxicity.

scholarly journals Possibility to Biotransform Anthracyclines by Peroxidases Produced by Bjerkandera adusta CCBAS 930 with Reduction of Geno- and Cytotoxicity and Pro-Oxidative Activity

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 462
Author(s):  
Kamila Rybczyńska-Tkaczyk ◽  
Teresa Korniłłowicz-Kowalska ◽  
Konrad A. Szychowski
Keyword(s):  
Biological Treatment ◽  
Vibrio Fischeri ◽  
Human Fibroblasts ◽  
White Rot ◽  
White Rot Fungus ◽  
Lepidium Sativum ◽  
Anthracycline Antibiotics ◽  
Anthraquinone Dyes ◽  
Before And After ◽  
Lepidium Sativum L

The aim of this study was to evaluate the bioremoval mechanism of anthracycline antibiotics by the white-rot fungus B. adusta CCBAS 930. The activity of oxidoreductases and levels of phenolic compounds and free radicals were determined during the biotransformation of anthraquinone antibiotics: daunomycin (DNR) and doxorubicin (DOX) by B. adusta strain CCBAS 930. Moreover, phytotoxicity (Lepidium sativum L.), ecotoxicity (Vibrio fischeri), genotoxicity and cytotoxicity of anthraquinone dyes were evaluated before and after biological treatment. More than 80% and 90% of DNR and DOX were removed by biodegradation (decolorization). Initial solutions of DNR and DOX were characterized by eco-, phyto-, geno- and cytotoxicity. Despite efficient decolorization, secondary metabolites, toxic to bacteria, formed during biotransformation of anthracycline antibiotics in B. adusta CCBAS 930 cultures. DNR and DOX metabolites did not increase reactive oxygen species (ROS) production in human fibroblasts and resazurin reduction. DNR metabolites did not change caspase-3 activity.

scholarly journals Biodecolorization of anthraquinone dyes using immobilised mycelium of Bjerkandera adusta CCBAS930

2020 ◽  
Vol 171 ◽  
pp. 01013 ◽  
Author(s):  
Kamila Rybczyńska-Tkaczyk ◽  
Teresa Korniłłowicz-Kowalska
Keyword(s):  
Phenolic Compounds ◽  
Lepidium Sativum ◽  
Carminic Acid ◽  
Secondary Products ◽  
Bjerkandera Adusta ◽  
Anthraquinone Dyes ◽  
Before And After ◽  
Acid Blue 129 ◽  
Acid Blue ◽  
Acid Green 25

The aim of this study was to characterize the activity of oxidoreductases during biotransformation of 0.01% anthraquinone dyes: Alizarin Blue Black B (ABBB) and Acid Blue 129 (AB129), Carminic Acid (CA), Remazol Brilliant Blue R (RBBR), Acid Green 25 (AG25) and Poly R-478 by immobilized strain of Bjerkandera adusta CCBAS 930. Phenolic compounds, phytotoxicity (Lepidium sativum L.), biotoxicity were evaluated to determine the toxicity of anthraquinone dyes before and after the treatment with immobilized B. adusta CCBAS 930. More than 60% of CA and AB129 were removed by immobilized B. adusta CCBAS after 7 days. No secondary products toxic to plants and bacteria were formed during immobilized cultures of B. adusta CCBAS 930.

scholarly journals Decolorization and biodegradation of melanoidin contained in beet molasses by an anamorphic strain of Bjerkandera adusta CCBAS930 and its mutants

2020 ◽  
Vol 37 (1) ◽  
Author(s):  
Teresa Korniłłowicz-Kowalska ◽  
Kamila Rybczyńska-Tkaczyk
Keyword(s):  
Free Radicals ◽  
Phenolic Compounds ◽  
Peroxidase Activity ◽  
Aerial Mycelium ◽  
Culture Method ◽  
White Rot ◽  
White Rot Fungus ◽  
Bjerkandera Adusta ◽  
Beet Molasses ◽  
Stimulation Of

AbstractWe used a ligninolytic strain of the white-rot fungus B. adusta CCBAS 930 and its mutants with modified ligninolytic activity to assess their potential to remove of molasses. The analyzed strains have been shown to be able to decolorize 1% or 2% molasses solutions containing brown-colored toxic melanoidins. It was found that the decolorization process was determined by the transition to the stage of production of sporulating aerial mycelium (liquid and agar cultures) coupled with an increase in peroxidase activity, which was accompanied by a decrease in the level of melanoidin, free radicals, and phenolic compounds. Four different peroxidase activities were detected in post-culture liquids, i.e. horseradish-like (HRP-like), manganese-dependent (MnP), lignin (LiP), and versatile (VP) peroxidase activities. The HRP-like peroxidase was characterized by the highest activity. The efficiency of removal of melanoidins from a 1% molasses solution by the parental strain and the mutants was dependent on the culture method. The highest efficiency was noted in immobilized cultures (threefold higher than in the mycelium-free cultures), which was accompanied by stimulation of HRP-like peroxidase activity. Mutant 930-5 was found to be the most effective in the decolorization and decomposition of melanoidin. The HRP-like activity in the immobilized cultures of B. adusta 930-5 was 640-fold higher than in the mycelium-free cultures of the fungus. Moreover, decolorization and biodegradation of melanoidin by B. adusta CCBAS 930 and 930-5 was coupled with detoxification. Graphic abstract

scholarly journals Superoxide dismutase isozyme activity and antioxidant responses of hydroponically cultured Lepidium sativum L. to NaCl stress

2013 ◽  
Vol 9 (1) ◽  
pp. 440-449 ◽  
Author(s):  
Arafet Manaa ◽  
Hajer Mimouni ◽  
Amel Terras ◽  
Farah Chebil ◽  
Salma Wasti ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Nacl Stress ◽  
Lepidium Sativum ◽  
Antioxidant Responses ◽  
Isozyme Activity ◽  
Lepidium Sativum L

scholarly journals Molecular identification and antimicrobial activities of some wild Egyptian mushrooms: Bjerkandera adusta as a promising source of bioactive antimicrobial phenolic compounds

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Elham R. S. Soliman ◽  
Heba El-Sayed
Keyword(s):  
Phenolic Compounds ◽  
Micrococcus Luteus ◽  
Cost Effective ◽  
Antimicrobial Activities ◽  
Bjerkandera Adusta ◽  
Internal Transcribed Spacer Region ◽  
Agrocybe Aegerita ◽  
Encoding Gene ◽  
Promising Source ◽  
Related Compounds

Abstract Background The discovery of potential, new cost-effective drug resources in the form of bioactive compounds from mushrooms is one way to control the resistant pathogens. In the present research, the fruiting bodies of five wild mushrooms were collected from Egypt and identified using internal transcribed spacer region (ITS) of the rRNA encoding gene and their phylogenetic relationships, antimicrobial activities, and biochemical and phenolic compounds were evaluated. Results The sequences revealed identity to Bjerkandera adusta, Cyclocybe cylindracea, Agrocybe aegerita, Chlorophyllum molybdites, and Lentinus squarrosulus in which Cyclocybe cylindracea and Agrocybe aegerita were closely related, while Chlorophyllum molybdites was far distant. Cyclocybe cylindracea and Agrocybe aegerita showed 100% similarity based on the sequenced ITS-rDNA fragment and dissimilar antimicrobial activities and chemical composition were detected. Bjerkandera adusta and Cyclocybe cylindracea showed strong antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Micrococcus luteus, Streptococcus pneumoniae, and Candida albicans. This activity could be attributed to the detected phenolic and related compounds’ contents. Conclusion Our finding provides a quick and robust implement for mushroom identification that would facilitate mushroom domestication and characterization for human benefit.

Toxicological effects of different-sized Co–Fe (CoFe2O4) nanoparticles on Lepidium sativum L.: towards better understanding of nanophytotoxicity

Ecotoxicology ◽  
2021 ◽  
Vol 30 (2) ◽  
pp. 277-291
Author(s):  
D. Montvydienė ◽  
A. Jagminas ◽  
Ž. Jurgelėnė ◽  
M. Kazlauskas ◽  
R. Butrimienė ◽  
...  
Keyword(s):  
Lepidium Sativum ◽  
Cofe2o4 Nanoparticles ◽  
Toxicological Effects ◽  
Lepidium Sativum L

scholarly journals Phytotoxicity of Tires Evaluated in Simulated Conditions

Environments ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 49
Author(s):  
Markéta Šourková ◽  
Dana Adamcová ◽  
Jan Winkler ◽  
Magdalena Daria Vaverková
Keyword(s):  
Higher Plants ◽  
Sinapis Alba ◽  
Lepidium Sativum ◽  
Root Growth Inhibition ◽  
Chemical Parameters ◽  
Sinapis Alba L ◽  
Test Kit ◽  
Physical And Chemical ◽  
Aqueous Leachate ◽  
Lepidium Sativum L

Illegal dumps and landfills with disposed of tires are a fact of today, which should not be neglected as they represent a great ecological burden for the environment, affect the surrounding nature and disturb the landscape. This research was focused on testing the phytotoxicity of aqueous leachates from the fractions of tires in two sets of experiments—to simulate laboratory conditions (tire leaching in distilled water) and natural conditions (tire leaching in water from a recipient) using the Phytotoxkit testing kit (kit for the establishment of inhibition/stimulation effect on the root development) and the watercress test of phytotoxicity (biological method for the assessment of leachate phytotoxicity). Plants whose seeds were selected for the test were watercress (Lepidium sativum L.) and white mustard (Sinapis alba L.). The aqueous leachate was tested for 38 weeks. During the experiment, physical and chemical parameters were measured at intervals of 14 days by the testing instrument HACH TEST KIT: electric conductivity (EC), amount of dissolved oxygen (LDO) and pH. Results of root growth inhibition (IR) on the seeds of Lepidium sativum L. and Sinapis alba L. exhibited values ranging from 11.73% to 47.74% in the tested samples. Results of germination index (GI) on the seeds of Lepidium sativum L. exhibited values below 66% in the tested samples, which indicated the leachate phytotoxicity. In spite of the fact that similar studies are tackling the acute toxicity of leachates from tires (particularly to algae, embryos and animals), this research brings complementary information in testing the acute phytotoxicity of tire leachates to higher plants.

Natürliche Hemmstoffe von Keimung und Wachstum, I Ausarbeitung eines quantitativen Biotests und Anwendung auf Extrakte aus Spelzen von Avena sativa L. / Natural Inhibitors of Germination and Growth, I. Development of a Quantitative Biotest and Application upon Extracts from Husks of Avena sativa L.

1982 ◽  
Vol 37 (9) ◽  
pp. 793-801 ◽  
Author(s):  
Rudolf Karl ◽  
Wolfhart Rüdiger
Keyword(s):  
Abscisic Acid ◽  
Root Growth ◽  
Organic Acids ◽  
Avena Sativa ◽  
Inhibitory Activity ◽  
Lepidium Sativum ◽  
Carbonic Acids ◽  
Germination And Growth ◽  
Natural Inhibitors ◽  
Lepidium Sativum L

Extracts from oat husks inhibit germination of a variety of seeds including Avena sativa L., Sorghum spec., Phalleris spec., Raphanus spec., Amaranthus caudatus, Lepidium sativum L. A quantitative assay for this inhibition was developed on the basis of percentage of root growth of Avena in the presence of extracted material compared with root growth of water controls. Fractionation of the extracts revealed that about half of the total inhibitory activity was found in the fraction of free organic acids. The inhibition was not due to known inhibitors. Abscisic acid. was not found in this extract. Phenole carbonic acids were determined in the extract. Their con­centration was too low to significantly contribute to the observed inhibitory activity.

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