Enhanced Pharmaceutically Active Compounds Productivity from Streptomyces SUK 25: Optimization, Characterization, Mechanism and Techno-Economic Analysis

The present research aimed to enhance the pharmaceutically active compounds’ (PhACs’) productivity from Streptomyces SUK 25 in submerged fermentation using response surface methodology (RSM) as a tool for optimization. Besides, the characteristics and mechanism of PhACs against methicillin-resistant Staphylococcus aureus were determined. Further, the techno-economic analysis of PhACs production was estimated. The independent factors include the following: incubation time, pH, temperature, shaker rotation speed, the concentration of glucose, mannitol, and asparagine, although the responses were the dry weight of crude extracts, minimum inhibitory concentration, and inhibition zone and were determined by RSM. The PhACs were characterized using GC-MS and FTIR, while the mechanism of action was determined using gene ontology extracted from DNA microarray data. The results revealed that the best operating parameters for the dry mass crude extracts production were 8.20 mg/L, the minimum inhibitory concentrations (MIC) value was 8.00 µg/mL, and an inhibition zone of 17.60 mm was determined after 12 days, pH 7, temperature 28 °C, shaker rotation speed 120 rpm, 1 g glucose /L, 3 g mannitol/L, and 0.5 g asparagine/L with R2 coefficient value of 0.70. The GC-MS and FTIR spectra confirmed the presence of 21 PhACs, and several functional groups were detected. The gene ontology revealed that 485 genes were upregulated and nine genes were downregulated. The specific and annual operation cost of the production of PhACs was U.S. Dollar (U.S.D) 48.61 per 100 mg compared to U.S.D 164.3/100 mg of the market price, indicating that it is economically cheaper than that at the market price.

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STEM measurement of subcellular water distributions

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100148678 ◽

1993 ◽

Vol 51 ◽

pp. 568-569

Author(s):

R.D. Leapman ◽

S.Q. Sun ◽

S-L. Shi ◽

R.A. Buchanan ◽

S.B. Andrews

Keyword(s):

Water Content ◽

Internal Standard ◽

Dry Weight ◽

Dry Mass ◽

Scanning Transmission Electron Microscope ◽

Dark Field ◽

Bulk Water ◽

Inelastic Electron ◽

Scanning Transmission ◽

Annular Dark Field

Recent advances in rapid-freezing and cryosectioning techniques coupled with use of the quantitative signals available in the scanning transmission electron microscope (STEM) can provide us with new methods for determining the water distributions of subcellular compartments. The water content is an important physiological quantity that reflects how fluid and electrolytes are regulated in the cell; it is also required to convert dry weight concentrations of ions obtained from x-ray microanalysis into the more relevant molar ionic concentrations. Here we compare the information about water concentrations from both elastic (annular dark-field) and inelastic (electron energy loss) scattering measurements.In order to utilize the elastic signal it is first necessary to increase contrast by removing the water from the cryosection. After dehydration the tissue can be digitally imaged under low-dose conditions, in the same way that STEM mass mapping of macromolecules is performed. The resulting pixel intensities are then converted into dry mass fractions by using an internal standard, e.g., the mean intensity of the whole image may be taken as representative of the bulk water content of the tissue.

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Study effect of crude extracts of Hibiscus sabdarifa L. on some Enterobacteriaceae

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v9ispl1.1302 ◽

2018 ◽

Vol 9 (SPL1) ◽

Author(s):

Sabreen A Kamal ◽

Ishraq A Salih ◽

Hawraa Jawad Kadhim ◽

Zainab A Tolaifeh

Keyword(s):

Escherichia Coli ◽

Ethyl Acetate ◽

Bacterial Growth ◽

Chemical Compounds ◽

Inhibition Zone ◽

Crude Extracts ◽

And Control

Red rose or roselle (beauty rose ) is natively known as red tea belong to Malvaceae, it is flowers use traditionally for antihypertensive hepato protective, anticancer,antidiabetic,antibacterial, cytotoxicity and antidiarreal, By preparing red tea from it's flower. In this study, we extract chemical compounds by using two solvent which are Ethanol, Ethyl acetate. so we can extract Anthocyanin which is responsible for red colour of flower with many chemical compounds. then study the effect of these extracts on 5 genera from Enterobacteriacaea which can cause diarrheae (Shigella, Salmonella, Escherichia coli, Proteus and Klebsiella ) by preparing 3 concentrations for each solvent (250, 500, 750 ) mg/ml, and control then compare with two antibiotic (Azereonam 30 mg/ml and Bacitracin 10 mg/ml ) these extracts revealed obvious inhibition zone in bacterial growth.

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Assessment of antioxidant and antibacterial activities of crude extracts of verbena officinalis Linn root or Atuch (Amharic)

10.31221/osf.io/pzh3s ◽

2019 ◽

Cited By ~ 1

Author(s):

Chem Int

Keyword(s):

Antioxidant Activity ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Dry Weight ◽

Antimicrobial Activities ◽

Total Flavonoid ◽

Root Extract ◽

Antioxidant Power ◽

Crude Extracts

Verbena officinalis Linn is a traditionally known medicinal plant which is used against a number of diseases including inflammatory conditions. In this study its antioxidant activity (reducing powers, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities), ferric reduction activity potential (FRAP), total flavonoid concentration and antimicrobial activities of 80%, 90%, 100% methanol and chloroform extracts of V. officinalis Linn root and 90% and100% methanol leaf extracts were determined. Its antioxidant activity increases with increase in amount of extract (10% to 40%v/v). Total flavonoid content (TFC) varied from 73.32±0.002 mgQE/100g of dry weight (90% methanol) to 42.39±0.032 mgQE/100g dry weight (chloroform), 2,2-diphenyl-1-picrylhydrazyl (DPPH), radical scavenging activity (%) was varied between 87.39% (90% methanol) to 45.57% (chloroform) while Ferric reducing antioxidant power was observed between 372.93±0.04 mgAAE/100 g extract (90% methanol) to 129.41±0.026 mgAAE/100 g chloroform in the root extract. The methanolic extract of the leaf showed less antioxidant activity than the methanolic extract of the root. Crude extracts of V. officinalis root showed various degree of antimicrobial activity towards drug resistance microbial pathogens. Growth inhibition tests against bacterial pathogens demonstrated concentration dependence. Moreover, gram positive bacteria were more susceptible to V. officinalis root extract when compared to gram negative bacteria. In general V. officinalis root and leave extracts possess strong antioxidant and antimicrobial activities.

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Pharmaceutically Active Compounds Degradation Using Doped TiO2 Functionalized Zeolite Photocatalyst

Revista de Chimie ◽

10.37358/rc.18.1.6040 ◽

2018 ◽

Vol 69 (1) ◽

pp. 34-37 ◽

Cited By ~ 1

Author(s):

Monica Ihos ◽

Corneliu Bogatu ◽

Carmen Lazau ◽

Florica Manea ◽

Rodica Pode

Keyword(s):

Organic Carbon ◽

Active Compound ◽

Uv Spectra ◽

Pharmaceutically Active Compounds ◽

Mineralization Process ◽

Active Compounds ◽

Spectra Analysis ◽

Doped Tio2 ◽

Toc Removal ◽

Pharmaceutically Active Compound

The aim of this study was the investigation of photocatalytic degradation of pharmaceutically active compounds using doped TiO2 functionalized zeolite photocatalyst. Diclofenac (DCF), a non-steroidal anti-inflammatory drug, that represents a biorefractory micropollutant, was chosen as model of pharmaceutically active compound. The photocatalyst was Z-TiO2-Ag. The concentration of DCF in the working solutions was 10 mg/L,50 mg/L,100 mg/L and 200 mg/L and of photocatalyst 1 g/L in any experiments. The process was monitored by recording the UV spectra of the treated solutions and total organic carbon (TOC) determination. The UV spectra analysis and TOC removal proved that along the advanced degradation of DCF also a mineralization process occurred. The carried out research provided useful information envisaging the treatment of pharmaceutical effluents by photocatalysis.

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