scholarly journals Comparison of Antioxidant and Anticancer Properties of Soft Coral-Derived Sinularin and Dihydrosinularin

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3853
Author(s):  
Sheng-Chieh Wang ◽  
Ruei-Nian Li ◽  
Li-Ching Lin ◽  
Jen-Yang Tang ◽  
Jui-Hsin Su ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Soft Coral ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Antioxidant Property ◽  
Radical Scavenger ◽  
Anticancer Properties ◽  
Liver Cancer Cells ◽  
Radical Scavenger Activity ◽  
Scavenger Activity

Marine natural products are abundant resources for antioxidants, but the antioxidant property of the soft corals-derived sinularin and dihydrosinularin were unknown. This study aimed to assess antioxidant potential and antiproliferation effects of above compounds on cancer cells, and to investigate the possible relationships between them. Results show that sinularin and dihydrosinularin promptly reacted with 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), and hydroxyl (•OH), demonstrating a general radical scavenger activity. Sinularin and dihydrosinularin also show an induction for Fe+3-reduction and Fe+2-chelating capacity which both strengthen their antioxidant activities. Importantly, sinularin shows higher antioxidant properties than dihydrosinularin. Moreover, 24 h ATP assays show that sinularin leads to higher antiproliferation of breast, lung, and liver cancer cells than dihydrosinularin. Therefore, the differential antioxidant properties of sinularin and dihydrosinularin may contribute to their differential anti-proliferation of different cancer cells.

scholarly journals HPLC-DAD Polyphenolic Profiling and Antioxidant Activities of Sorghum bicolor during Germination

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 417
Author(s):  
Ioana Otilia Ghinea ◽  
Maria Daniela Ionica Mihaila ◽  
Giorgiana-Valentina Blaga (Costea) ◽  
Sorin Marius Avramescu ◽  
Mihaela Cudalbeanu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Sorghum Bicolor ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Ultrasound Assisted Extraction ◽  
Food Ingredient ◽  
Radical Scavenger Activity ◽  
Scavenger Activity ◽  
Sorghum Grains

The purpose of this study was to assess the suitability of the Romanian Albanus hybrid of Sorghum bicolor as a potential functional food ingredient. Ultrasound-assisted extraction in different solvents, together with spectrophotometric and chromatographic methods, was used to monitor the variation in total phenolic and flavonoid content and the antioxidant activity of raw sorghum grains before and during short germination periods (24, 36 and 48 h). The High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) investigation of the extracts revealed that the ungerminated sorghum grains extracted with methanol had the highest diversity of phenolic compounds, while the total phenolic content (TPC) was the highest after 36 h of germination in both extract types: 1853 mg GAE/100 g for the methanolic extract and 1726 mg GAE/100 g for the ethanolic extract. The findings of this study showed that the TPC of sorghum extracts is strongly correlated with their antioxidant activity and, overall, that the studied extracts presented a good radical scavenger activity, which supports the benefits of alimentary uses of Sorghum bicolor grains.

scholarly journals Polyphenol Composition and Antioxidant Capacity of Three Lysimachia Species

2014 ◽  
Vol 9 (10) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Anita Toth ◽  
Gergo Toth ◽  
Agnes Kery
Keyword(s):  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Flavonoid Glycosides ◽  
Radical Scavenger ◽  
Polyphenol Content ◽  
Radical Scavenger Activity ◽  
External Standard Method ◽  
Scavenger Activity ◽  
Column Chromatographic

The polyphenol composition and antioxidant properties of three Lysimachia species ( L. nummularia L., L. vulgaris L. and L. punctata L.) and their column chromatographic fractions were investigated. The antioxidant activity of herb extracts and 54 different column chromatographic fractions was evaluated using in vitro DPPH• and ABTS•+ decolorization tests. The total polyphenol content was determined by spectrophotometric methods. The phenolic compounds of extracts of different Lysimachia species and their bioactive fractions were characterized by online chromatographic methods. For identification of the compounds, UV spectral data, accurate molecular mass and formula, as well as MS and fragmentation patterns given by LC-DAD-ESI/MS/MS and LC-ESI-TOF analyses were used. Quantification of the compounds was performed by LC-DAD using an external standard method. In the extracts, caffeic acid derivatives, chlorogenic acid, free flavonoid aglycones, and 11 various flavonoid glycosides were identified. Flavonoid composition of Lysimachia extracts showed significant differences. L. punctata extracts had the strongest DPPH• and ABTS•+ radical scavenger activity (IC50=43.3 μg/mL and 21.3 μg/mL), due to their high myricitrin and quercetin-hexoside content. Correlation between polyphenol content and radical scavenging activity of each column chromatographic fractions is also included.

scholarly journals Acetylcholinesterase Inhibition and Antioxidant Activity of Essential Oils from Schinus Areira L. and Schinus Longifolia (Lindl.) Speg

2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ana P. Murray ◽  
María S. Vela Gurovic ◽  
Silvana A. Rodriguez ◽  
María G. Murray ◽  
Adriana A. Ferrero
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Acetylcholinesterase Inhibition ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Scavenger Activity

The essential oils of Schinus areira L. and S. longifolia (Lindl.) Speg. (Anacardiaceae) have been studied for their in vitro anti-acetylcholinesterase and antioxidant activities. The chemical composition of the oils obtained by hydrodistillation was determined by GC-MS. Fruit and leaf oils of S. areira were analyzed separately. The essential oil from S. longifolia elicited marked enzymatic inhibition (IC50 = 20.0 ± 1.0 μg/mL) and showed radical scavenger activity (IC50 = 25.2 ± 2.4 μg/mL). The essential oil from S. areira leaves was more active than that of the fruits in both bioassays.

Antioxidant Activity of Edible Fungi (Truffles and Mushrooms): Losses during Industrial Processing

2002 ◽  
Vol 65 (10) ◽  
pp. 1614-1622 ◽  
Author(s):  
M. ANTONIA MURCIA ◽  
MAGDALENA MARTÍNEZ-TOMÉ ◽  
ANTONIA M. JIMÉNEZ ◽  
ANA M. VERA ◽  
MARIO HONRUBIA ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Radical Scavenger ◽  
Edible Fungi ◽  
Industrial Processing ◽  
Radical Scavenger Activity ◽  
Scavenger Activity ◽  
Significant Difference

The antioxidant properties of two raw truffles (Terfezia claveryi Chatin and Picoa juniperi Vittadini) and five raw mushrooms (Lepista nuda, Lentinus edodes, Agrocybe cylindracea, Cantharellus lutescens, and Hydnum repandum) were tested by subjecting these truffles and mushrooms to different industrial processes (freezing and canning) and comparing them with common food antioxidants (α-tocopherol [E-307], BHA [E-320], BHT [E-321], and propyl gallate [E-310]) with regard to their ability to inhibit lipid oxidation. All of the truffles and mushrooms analyzed exhibited higher percentages of oxidation inhibition than did the food antioxidants according to assays based on lipid peroxidation (LOO•), deoxyribose (OH•), and peroxidase (H2O2). Frozen samples exhibited a small reduction in free radical scavenger activity, but the results did not show a significant difference (P < 0.05) with respect to the raw samples, while canned truffles and mushrooms lost some antioxidant activity as a consequence of industrial processing. All of the raw and frozen truffles and mushrooms except frozen Cantharellus improved the stability of oil against oxidation (100°C Rancimat), while canned samples accelerated oil degradation. Antioxidant activity during 30 days of storage was measured by the linoleic acid assay, and all of the samples except canned Terfezia, Picoa, and Hydnum showed high or medium antioxidant activity. The Trolox equivalent antioxidant capacity assay was used to provide a ranking order of antioxidant activity as measured against that of Trolox (a standard solution used to evaluate equivalent antioxidant capacity). The order of raw samples with regard to antioxidant capacity was as follows (in decreasing order): Cantharellus, Agrocybe, Lentinus, Terfezia, Picoa, Lepista, and Hydnum. Losses of antioxidant activity were detected in the processed samples of these truffles and mushrooms.

scholarly journals Ocimum campechianum Mill. from Amazonian Ecuador: Chemical Composition and Biological Activities of Extracts and Their Main Constituents (Eugenol and Rosmarinic Acid)

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 84
Author(s):  
Massimo Tacchini ◽  
Monica Paulina Echeverria Guevara ◽  
Alessandro Grandini ◽  
Immacolata Maresca ◽  
Matteo Radice ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Pseudomonas Syringae ◽  
Rosmarinic Acid ◽  
Plant Protection ◽  
Biological Activities ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Antioxidant Agents ◽  
Scavenger Activity

The essential oil (EO), the methanolic (MeOH), and the 70% ethanolic (70% EtOH) extracts obtained from the aerial parts of Ocimum campechianum Mill. (Ecuador) were chemically characterized through gas-chromatography coupled to mass spectrometry detector (GC-MS), high-performance liquid chromatography coupled to diode array-mass spectrometry detectors (HPLC-DAD-MS) and studied for their in vitro biological activity. The radical scavenger activity, performed by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, highlighted significant IC50 values for the EO, extracts and their main constituents (eugenol and rosmarinic acid). EO (and eugenol) showed noteworthy activity against Pseudomonas syringae pv. syringae and a moderate effect against clinical Candida strains, with possible synergism in association to fluconazole against the latter microorganisms. The extracts and pure molecules exhibited weak cytotoxic activity against the HaCat cell line and no mutagenicity against Salmonella typhimurium TA98 and TA100 strains, giving indication of safety. Instead, EO showed a weak activity against adenocarcinomic human alveolar basal epithelial cells (A549). The above-mentioned evidence leads us to suggest a potential use of the crude drug, extracts, and EO in cosmetic formulation and food supplements as antioxidant agents. In addition, EO may also have a possible application in plant protection and anti-Candida formulations.

scholarly journals Gastroprotective Value of Berries: Evidences from Methanolic Extracts of Morus nigra and Rubus niveus Fruits

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Luciane Angela Nottar Nesello ◽  
Maria Luisa Maes Lima Beleza ◽  
Marihá Mariot ◽  
Luísa Nathália Bolda Mariano ◽  
Priscila de Souza ◽  
...  
Keyword(s):  
Atpase Activity ◽  
Free Radical Scavenger ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Lipid Hydroperoxides ◽  
Ulcer Area ◽  
Morus Nigra ◽  
Radical Scavenger Activity ◽  
Scavenger Activity

This study evaluated the gastroprotective value of the methanol extracts from fruits of Morus nigra L. (black mulberry (MEMN)) and Rubus niveus Thunb (raspberry (MERN)). The total phenolic compounds and flavonoids were measured, as well as the in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenger activity. The gastroprotective effects of the extracts against 60% ethanol/0.3 M HCl were evaluated in mice. After that, the lipid hydroperoxides and reduced glutathione levels at ulcerated tissue were determined. The effects of extracts on H+/K+-ATPase activity were also verified. The extracts exhibited high contents of polyphenols; however, MERN presented 1.5-fold higher levels. The presence of flavonoids also was confirmed. In addition, MEMN (IC50 = 13.74 μg/mL) and MERN (IC50 = 14.97 μg/mL) scavenged DPPH radical. The MEMN reduced the ulcer area only at 300 mg/kg (p.o.) by 64.06%. Interestingly, MERN decreased the ulcer area in a superior potency (ED50 = 20.88 mg/kg), reducing the ulcer area by 81.86% at 300 mg/kg, and increased the gastric mucin levels. The antioxidant effects of extracts were evidenced by reduced lipoperoxides and increased reduction of glutathione amount in the gastric mucosa. However, MEMN or MERN did not change the H+/K+-ATPase activity. These results confirm that M. nigra and R. niveus are berries with a gastroprotective value by strengthening of gastric protective factors.

Sesquiterpene lactones fromLychnophora pohlii: neutrophil chemiluminescence inhibition and free radical scavenger activity

2006 ◽  
Vol 58 (6) ◽  
pp. 853-858 ◽  
Author(s):  
Alexandre Kanashiro ◽  
Luciana M. Kabeya ◽  
Cristiane F. F. Grael ◽  
Christiane O. Jordão ◽  
Ana Elisa C.S. Azzolini ◽  
...  
Keyword(s):  
Free Radical ◽  
Sesquiterpene Lactones ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Scavenger Activity

scholarly journals Effect of Nanoconfinement of Polyphenolic Extract from Grape Pomace into Functionalized Mesoporous Silica on Its Biocompatibility and Radical Scavenging Activity

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 696 ◽  
Author(s):  
Ana-Maria Brezoiu ◽  
Laura Bajenaru ◽  
Daniela Berger ◽  
Raul-Augustin Mitran ◽  
Mihaela Deaconu ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Radical Scavenging ◽  
Phosphate Buffer Solution ◽  
Grape Pomace ◽  
Array Detector ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Polyphenolic Extract ◽  
Scavenger Activity ◽  
Mcm 41

The aim of this paper is to assess the properties of Mamaia (MM) grape pomace polyphenolic extract loaded onto pristine and functionalized MCM-41 mesoporous silica as potential ingredients for nutraceuticals or cosmetics. The chemical profile of hydroalcoholic polyphenolic extracts, prepared either by conventional extraction or microwave-assisted method, was analyzed by reverse-phase high-performance liquid chromatography with photodiode array detector (HPLC-PDA) analysis, while their radical scavenger activity (RSA) was evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl radical) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) assays. The extract-loaded materials were characterized by Fourier transform infrared (FTIR) spectroscopy, N2 adsorption-desorption isotherms, thermogravimetric analysis, as well as RSA (DPPH and ABTS assays). The polyphenols release profiles from pristine and functionalized (with mercaptopropyl, propyl sulfonic acid, cyanoethyl and propionic acid moieties) MCM-41-type supports were determined in phosphate buffer solution (PBS) pH 5.7. For selected materials containing embedded phytochemicals, cellular viability, and oxidative stress level on immortalized mouse embryonic fibroblast cell line (NIH3T3) were evaluated. A more acidic functional groups linked on silica pore walls determined a higher amount of phytochemicals released in PBS. The extract-loaded materials showed a good cytocompatibility on tested concentrations. The embedded extract preserved better the RSA over time than the free extract. The polyphenols-loaded MCM-41-type silica materials, especially MM@MCM-COOH material, demonstrated a good in vitro antioxidant effect on NIH3T3 cells, being potential candidates for nutraceutical or cosmetic formulations.

scholarly journals Black goji berry (Lycium ruthenicum) tea has higher phytochemical contents and in vitro antioxidant properties than red goji berry (Lycium barbarum) tea

2020 ◽  
Vol 4 (4) ◽  
pp. 193-201
Author(s):  
Bei Liu ◽  
Qingqing Xu ◽  
Yujing Sun
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Antioxidant Property ◽  
Lycium Barbarum ◽  
Antioxidant Power ◽  
Goji Berry ◽  
Good Habit

Abstract Goji berry tea, a traditional herbal tea, is the main ate mode of goji berry in Asia, yet few studies in comparison with red goji berry tea and black goji berry tea are carried out. This study investigated the effects of water temperature and soak time on the colour, phytochemicals, and the antioxidant capacity [2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), and the ferric-reducing antioxidant power (FRAP)] of two goji berry tea. A comparison of the bioactive compounds and antioxidant activities between black and red goji berry tea was conducted. Results showed that both red and black goji berry tea were rich in phytochemicals, giving high antioxidant ability. The levels of bioactive compounds and the antioxidant activity of the two goji berry tea increased as the increases in soak temperature and time. Black goji berry tea had higher phytochemicals and antioxidant property than those of red goji berry tea. Infused at 100° water for the same time, the levels of total polysaccharides (150 mg/100 ml), total polyphenols (238 mg/ml), and antioxidant capacity (550 μmol/100 ml) of black goji berry tea were 3.5, 2, and 5 times higher, respectively, in comparison with red goji berry tea. The results of this study demonstrate that hot drink of goji berry in China is a good habit and black goji berry tea may be a better choice.

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