scholarly journals Effects of Light Intensity and Spectral Composition on the Transcriptome Profiles of Leaves in Shade Grown Tea Plants (Camellia sinensis L.) and Regulatory Network of Flavonoid Biosynthesis

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5836
Author(s):  
Jian-Hui Ye ◽  
Yi-Qing Lv ◽  
Sheng-Rui Liu ◽  
Jing Jin ◽  
Yue-Fei Wang ◽  
...  
Keyword(s):  
Light Intensity ◽  
Spectral Composition ◽  
Light Signal ◽  
Flavonoid Biosynthesis ◽  
Flavonol Glycosides ◽  
Tea Leaves ◽  
Shade Treatment ◽  
Signal Perception ◽  
Tea Plants ◽  
Changing Light

Black net shade treatment attenuates flavonoid biosynthesis in tea plants, while the effect of light quality is still unclear. We investigated the flavonoid and transcriptome profiles of tea leaves under different light conditions, using black nets with different shade percentages, blue, yellow and red nets to alter the light intensity and light spectral composition in the fields. Flavonol glycosides are more sensitive to light intensity than catechins, with a reduction percentage of total flavonol glycosides up to 79.6% compared with 38.7% of total catechins under shade treatment. A total of 29,292 unigenes were identified, and the KEGG result indicated that flavonoid biosynthesis was regulated by both light intensity and light spectral composition while phytohormone signal transduction was modulated under blue net shade treatment. PAL, CHS, and F3H were transcriptionally downregulated with light intensity. Co-expression analysis showed the expressions of key transcription factors MYB12, MYB86, C1, MYB4, KTN80.4, and light signal perception and signaling genes (UVR8, HY5) had correlations with the contents of certain flavonoids (p < 0.05). The level of abscisic acid in tea leaves was elevated under shade treatment, with a negative correlation with TFG content (p < 0.05). This work provides a potential route of changing light intensity and spectral composition in the field to alter the compositions of flavor substances in tea leaves and regulate plant growth, which is instructive to the production of summer/autumn tea and matcha.

scholarly journals Review: the effect of light on the key pigment compounds of photosensitive etiolated tea plant

2021 ◽  
Vol 62 (1) ◽  
Author(s):  
Cuinan Yue ◽  
Zhihui Wang ◽  
Puxiang Yang
Keyword(s):  
Light Intensity ◽  
Tea Plant ◽  
Light Signal ◽  
Tea Leaves ◽  
Leaf Color ◽  
Strong Light ◽  
High Amino Acid ◽  
The Difference ◽  
Coproporphyrinogen Iii Oxidase ◽  
Tea Plants

Abstract Background Light is the ultimate energy source of plant photosynthesis, which has an important impact on the growth, development, physiology and biochemistry of tea plant. Photosensitive etiolated tea plant belongs to a kind of colored leaf plant, which is a physiological response to light intensity. Compared with conventional green bud and leaf of tea plant, the accumulation of pigment compounds (chlorophyll and carotenoids, etc.) closely related to a series of reactions of photosynthesis in photosensitive etiolated tea plant is reduced, resulting in the difference of leaf color of tea. This specific tea resource has high application value, among which high amino acid is one of its advantages. It can be used to process high-quality green tea with delicious taste and attractive aroma, which has been widely attention. The mechanism of the color presentation of the etiolated mutant tea leaves has been given a high topic and attention, especially, what changes have taken place in the pigment compounds of tea leaves caused by light, which makes the leaves so yellow. At present, there have been a lot of research and reports. Purpose of the review We describe the metabolism and differential accumulation of key pigment compounds affecting the leaf color of photosensitive etiolated tea that are triggered by light, and discuss the different metabolism and key regulatory sites of these pigments in different light environments in order to understand the “discoloration” matrix and mechanism of etiolated tea resources, answer the scientific question between leaf color and light. It provides an important strategy for artificial intervention of discoloration of colored tea plant. Conclusion The differential accumulation of pigment compounds in tea plant can be induced phytochrome in response to the change of light signal. The synthesis of chlorophyll in photoetiolated tea plants is hindered by strong light, among which, the sites regulated by coproporphyrinogen III oxidase and chlorophyllide a oxidase is sensitive to light and can be inhibited by strong light, resulting in the aggravation of leaf etiolation. The phenomenon can be disappeared or weakened by shading or reducing light intensity, and the leaf color is greenish, but the increase of chlorophyll-b accumulation is more than that of chlorophyll-a. The synthesis of carotenoids is inhibited strong light, and high the accumulation of carotenoids is reduced by shading. Most of the genes regulating carotenoids are up-regulated by moderate shading and down-regulated by excessive shading. Therefore, the accumulation of these two types of pigments in photosensitive etiolated tea plants is closely related to the light environment, and the leaf color phenotype shape of photosensitive etiolated tea plants can be changed by different light conditions, which provides an important strategy for the production and management of tea plant.

scholarly journals In search of the spectral composition of an effective light trap for the mushroom pest Lycoriella ingenua (Diptera: Sciaridae)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sándor Kecskeméti ◽  
András Geösel ◽  
József Fail ◽  
Ádám Egri
Keyword(s):  
Light Intensity ◽  
Spectral Sensitivity ◽  
Fungal Pathogens ◽  
Light Trap ◽  
Spectral Composition ◽  
Wavelength Dependence ◽  
Crop Damage ◽  
Photon Flux ◽  
Compound Eyes ◽  
Behavioural Experiments

AbstractCertain fungus gnats, like Lycoriella ingenua are notorious pests in agriculture, especially in mushroom production. While larvae cause mainly direct crop damage, adults are vectors of several dangerous fungal pathogens. To promote the development of pesticide-free management methods, such as light trapping, we measured the spectral sensitivity of L. ingenua compound eyes with electroretinography and performed two different behavioural experiments to reveal the wavelength dependence of phototaxis in this species. The spectral sensitivity of the compound eyes is bimodal with peaks at 370 nm (UV) and 526 nm (green). Behavioural experiments showed that attraction to light as a function of wavelength depends on light intensity. In our first experiment, where the minimal photon flux (105–109 photons/cm2/s) needed for eliciting a phototactic response was determined wavelength by wavelength, phototaxis was strongest in the green spectral range (~526 nm). In the other behavioural experiment, where wavelength preference was tested under a higher but constant light intensity (~1013 photons/cm2/s), the highest attraction was elicited by UV wavelengths (398 nm). Our results suggest that both UV and green are important spectral regions for L. ingenua thus we recommend to use both UV (~370-398 nm) and green (~526 nm) for trapping these insects.

scholarly journals PHOTOKINESIS AND TONIC EFFECT OF LIGHT IN UNIONICOLA

1932 ◽  
Vol 16 (2) ◽  
pp. 349-355 ◽  
Author(s):  
John H. Welsh
Keyword(s):  
Light Intensity ◽  
Simple Relation ◽  
Combined Effect ◽  
Direct Proportion ◽  
Water Mite ◽  
Locomotor Muscles ◽  
Leg Movement ◽  
Changing Light ◽  
Effect Of Light

1. The speed of progression of Unionicola, a water mite, is influenced by light; and over a certain range increases as a function of the light intensity. 2. The relation between speed and light intensity is not a simple one, as the speed of progression is due to the combined effect of amplitude of steps and frequency of leg movement. 3. The amplitude of stride increases in direct proportion to the logarithm of the light intensity, while the frequency of stepping has no such simple relation to intensity. 4. The change in length of stride with changing light intensity indicates a tonic effect of light on the locomotor muscles. Such an effect has been observed previously in studies of orientation, due to unequal illumination, which produces changes in posture.

scholarly journals PSB27: A thylakoid protein enabling Arabidopsis to adapt to changing light intensity

2015 ◽  
Vol 112 (5) ◽  
pp. 1613-1618 ◽  
Author(s):  
Xin Hou ◽  
Aigen Fu ◽  
Veder J. Garcia ◽  
Bob B. Buchanan ◽  
Sheng Luan
Keyword(s):  
Photosystem Ii ◽  
Light Intensity ◽  
Light Adaptation ◽  
Light Exposure ◽  
Starch Accumulation ◽  
State Transitions ◽  
Potential Candidate ◽  
Subsequent Work ◽  
Variable Intensity ◽  
Changing Light

In earlier studies we have identified FKBP20-2 and CYP38 as soluble proteins of the chloroplast thylakoid lumen that are required for the formation of photosystem II supercomplexes (PSII SCs). Subsequent work has identified another potential candidate functional in SC formation (PSB27). We have followed up on this possibility and isolated mutants defective in the PSB27 gene. In addition to lack of PSII SCs, mutant plants were severely stunted when cultivated with light of variable intensity. The stunted growth was associated with lower PSII efficiency and defective starch accumulation. In response to high light exposure, the mutant plants also displayed enhanced ROS production, leading to decreased biosynthesis of anthocyanin. Unexpectedly, we detected a second defect in the mutant, namely in CP26, an antenna protein known to be required for the formation of PSII SCs that has been linked to state transitions. Lack of PSII SCs was found to be independent of PSB27, but was due to a mutation in the previously described cp26 gene that we found had no effect on light adaptation. The present results suggest that PSII SCs, despite being required for state transitions, are not associated with acclimation to changing light intensity. Our results are consistent with the conclusion that PSB27 plays an essential role in enabling plants to adapt to fluctuating light intensity through a mechanism distinct from photosystem II supercomplexes and state transitions.

Effect of light intensity on assimilation characteristics of detached tea leaves

1964 ◽  
Vol 63 (2) ◽  
pp. 265-271 ◽  
Author(s):  
D. N. Barua
Keyword(s):  
Light Intensity ◽  
Chlorophyll Concentration ◽  
Leaf Lamina ◽  
Tea Leaves ◽  
Tungsten Filament ◽  
Strong Light ◽  
Filament Lamp ◽  
Sun Leaves ◽  
Different Sources ◽  
Effect Of Light

Photosynthetic rates of detached, mature tea leaves from four different sources were determined in excess CO2 and light of 4, 10, 16 and 32 klux intensities from a tungsten-filament lamp. Temperature was maintained at 25°C. The assimilation rates were significantly different for the four sources both in weak and in strong light. Neither thickness of the leaf lamina nor chlorophyll concentration could explain the cause of this difference.The effect of shade adaptation on the subsequent rate of assimilation was examined in one of the four sources of leaf. Shade-adapted leaves had significantly higher rates of photosynthesis at the weakest light intensity of 4 klux and lower rates in 16 and 32 klux intensities than the corresponding sun leaves.

scholarly journals Elaborate pupils in skates may help camouflage the eye

2018 ◽  
Author(s):  
Sean Youn ◽  
Corey Okinaka ◽  
Lydia M Mäthger
Keyword(s):  
Light Intensity ◽  
Spatial Frequency ◽  
Experimental Evidence ◽  
Pupillary Response ◽  
Pupil Dilation ◽  
Primary Factor ◽  
Natural Substrate ◽  
Spatial Frequencies ◽  
Pupillary Responses ◽  
Changing Light

AbstractThe little skate Leucoraja erinacea has elaborately shaped pupils, whose characteristics and functions have not been studied extensively. It has been suggested that such pupil shapes may camouflage the eye; yet, no experimental evidence has been presented to support this claim. Skates are bottom-dwellers that often bury into the substrate with their eyes protruding. If these pupils serve any camouflage function, we expect there to be a pupillary response related to the spatial frequency (“graininess”) of the background against which the eye is viewed. Here, we tested whether skate pupils dilate or constrict in response to background spatial frequency. We placed skates on background substrates with different spatial frequencies and recorded pupillary responses at three light intensities. In experiment 1, the skates’ pupillary response to three artificial checkerboards of different spatial frequencies was recorded. Skates responded to changing light intensity with pupil dilation/constriction; yet, their pupils did not change in response to spatial frequency. In experiment 2, in which skates could bury into three natural substrates with different spatial frequencies, such that their eyes protruded above the substrate, the pupils showed a subtle but statistically significant response to changes in substrate spatial frequency. Given the same light intensity, the smaller the spatial frequency of the natural substrate, the more constricted the pupil. While light intensity is the primary factor determining pupil dilation, these experiments are the first to show that pupils also change in response to background spatial frequency, which suggests that the pupil may aid in camouflaging the eye.

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