scholarly journals Lycoperoside H, a Tomato Seed Saponin, Improves Epidermal Dehydration by Increasing Ceramide in the Stratum Corneum and Steroidal Anti-Inflammatory Effect

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5860
Author(s):  
Shogo Takeda ◽  
Kenchi Miyasaka ◽  
Sarita Shrestha ◽  
Yoshiaki Manse ◽  
Toshio Morikawa ◽  
...  

Tomatoes are widely consumed, however, studies on tomato seeds are limited. In this study, we isolated 11 compounds including saponins and flavonol glycosides from tomato seeds and evaluated their effects on epidermal hydration. Among the isolated compounds, tomato seed saponins (10 µM) significantly increased the mRNA expression of proteins related to epidermal hydration, including filaggrin, involucrin, and enzymes for ceramide synthesis, by 1.32- to 1.91-fold compared with the control in HaCaT cells. Tomato seed saponins (10 µM) also decreased transepidermal water loss by 7 to 13 g/m2·h in the reconstructed human epidermal keratinization (RHEK) models. Quantitative analysis of the ceramide content in the stratum corneum (SC) revealed that lycoperoside H (1–10 µM) is a promising candidate to stimulate ceramide synthesis via the upregulation of ceramide synthase-3, glucosylceramide synthase, and β-glucocerebrosidase, which led to an increase in the total SC ceramides (approximately 1.5-fold) in concert with ceramide (NP) (approximately 2-fold) in the RHEK models. Evaluation of the anti-inflammatory and anti-allergic effects of lycoperoside H demonstrated that lycoperoside H is suggested to act as a partial agonist of the glucocorticoid receptor and exhibits anti-inflammatory effects (10 mg/kg in animal test). These findings indicate that lycoperoside H can improve epidermal dehydration and suppress inflammation by increasing SC ceramide and steroidal anti-inflammatory activity.

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1832
Author(s):  
Fabian Gendrisch ◽  
Anna Nováčková ◽  
Michaela Sochorová ◽  
Birgit Haarhaus ◽  
Kateřina Vávrová ◽  
...  

Gentiana lutea is a bitter herb that is traditionally used to improve gastric disorders. Recently, we have shown that Gentiana lutea extract (GE) also modulates the lipid metabolism of human keratinocytes in vitro and in vivo. In the present study, we investigated the role of GE on ceramide synthesis in human primary keratinocytes (HPKs) and psoriasis-like keratinocytes. We could demonstrate that GE increased the concentrations of glucosylceramides and the ceramide AS/AdS subclass without affecting the overall ceramide content in HPKs. The expression of ceramide synthase 3 (CERS3) and elongases (ELOVL1 and 4) was reduced in psoriasis lesions compared to healthy skin. Psoriasis-like HPKs, generated by stimulating HPKs with cytokines that are involved in the pathogenesis of psoriasis (IL-17, TNF-α, IL-22 and IFN-γ) showed increased levels of IL-6, IL-8 and increased expression of DEFB4A, as well as decreased expression of ELOVL4. The treatment with GE partly rescued the reduced expression of ELOVL4 in psoriasis-like HPKs and augmented CERS3 expression. This study has shown that GE modulates ceramide synthesis in keratinocytes. Therefore, GE might be a novel topical treatment for skin diseases with an altered lipid composition such as psoriasis.


Author(s):  
Mohammed M Mohammed

Osteoarthritis (OA)is a degenerative joint disease caused by gradual loss of cartilage. Telmisartan is an Angiotensin II receptor antagonist,and act as a partial agonist on the nuclear peroxisome proliferator-activated receptor-gamma (PPAR-γ),that has been reported to exert anti-inflammatory effects. This study was designed to evaluate the potential anti-inflammatory effect oftelmisartan in patients with knee OA.42 patients with painful knee OA were allocated into 2 groups,group (1): patients treated with naproxen tablets (500 mg/12 hr),telmisartan tablets (40 mg/day) and omeprazole (20 mg/day) for 3 months,while group (2): patients treated with naproxen tablets (500 mg/12hr) and omeprazole (20 mg/day) for 3 months. The serum levels of IL-1β,high-sensitivity C-reactive protein (hs-CRP),TNF-α anderythrocyte sedimentation rate (ESR) were measured before and after 3 months of treatment.Telmisartan when used in combination with naproxen resulted in significant decrease in serum levels of IL-1β and hs-CRP,higher than that produced by naproxen when used alone. The mean TNF-α level and ESR was decreased non-significantly in both study groups. Administration of telmisartan as an adjuvant therapy to naproxen in knee OA patients produced a significant decrease in the serum levels of IL-1β and hs-CRP,though no clear effect on TNF-α and ESR was noticed after 3 months treatment. Accordingly,many promising preventive strategies emerged from the available results since telmisartan relatively reduce the inflammatory burden in OA patients.


Author(s):  
Samira Salem ◽  
Essaid Leghouchi ◽  
Rachid Soulimani ◽  
Jaouad Bouayed

Abstract. Paw edema volume reduction is a useful marker in determining the anti-inflammatory effect of drugs and plant extracts in carrageenan-induced acute inflammation. In this study, the anti-inflammatory effect of Lobaria pulmonaria (LP) and Parmelia caperata (PC), two lichen species, was examined in carrageenan-induced mouse paw edema test. Compared to the controls in carrageenan-induced inflammation (n = 5/group), our results showed that pretreatment by single oral doses with PC extract (50–500 mg/kg) gives better results than LP extract (50–500 mg/kg) in terms of anti-edematous activity, as after 4 h of carrageenan subplantar injection, paw edema formation was inhibited at 82–99% by PC while at 35–49% by LP. The higher anti-inflammatory effect of PC, at all doses, was also observed on the time-course of carrageenan-induced paw edema, displaying profile closely similar to that obtained with diclofenac (25 mg/kg), an anti-inflammatory drug reference (all p < 0.001). Both LP and PC, at all doses, significantly ameliorated liver catalase (CAT) activity (all p < 0.05). However, superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity and glutathione (GSH) levels were found increased in liver of PC- compared to LP-carrageenan-injected mice. Our findings demonstrated on one hand higher preventive effects of PC compared to LP in a mouse carrageenan-induced inflammatory model and suggested, on the other hand, that anti-inflammatory effects elicited by the two lichens were closely associated with the amelioration in the endogenous antioxidant status of liver.


Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
AH Mollik ◽  
W Mozammel Haq ◽  
S Chandra Bachar ◽  
R Jahan ◽  
M Rahmatullah

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
WM Shaik Mossadeq ◽  
K Syamimi ◽  
MP Azyyati ◽  
ZA Zakaria ◽  
AK Arifah ◽  
...  

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
IST Figueiredo ◽  
FT Benevides ◽  
NMS Queiroz ◽  
LM Marques ◽  
TFG Souza ◽  
...  

Phlebologie ◽  
2008 ◽  
Vol 37 (05) ◽  
pp. 259-265 ◽  
Author(s):  
H. Kutzner ◽  
G. Hesse

SummaryThe reason of the so called ulcerated capillaritis alba or idiopathic atrophie blanche is vasculopathy caused by severe venous hypertension. Thrombosed and rarificated vessels worsen the oxygenation, increase permanent inflammation and impede the necessary compression therapy. The anti-inflammatory effects of heparin alleviate pain and being independent from the antithrombotic ones it needs much lower doses for treatment. This anti-inflammatory effect is now becoming more important in clinical phlebology. Case studies of more than 50 patients and one prospective randomized study of 87 patients clearly demonstrate the ameliorated healing of ulcerated atrophie blanche. In our office we could document this positive effect with 22 patients. We present the pathophysiology of low molecular heparins for ulcerated capillaritis alba and our own experiences with it.


Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
S Popov ◽  
G Popova ◽  
V Golovchenko ◽  
R Ovodova

2008 ◽  
Vol 29 (S 1) ◽  
Author(s):  
K Nieber ◽  
S Michael ◽  
K Grötzinger ◽  
JW Rauwald ◽  
O Kelber

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