scholarly journals Anti-Hyperglycemic Effects of Refined Fractions from Cyclocarya Paliurus Leaves on Streptozotocin-Induced Diabetic Mice

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6886
Author(s):  
Zheling Feng ◽  
Zhujun Fang ◽  
Cheng Chen ◽  
Chi Teng Vong ◽  
Jiali Chen ◽  
...  
Keyword(s):  
Water Extract ◽  
Ethanol Extract ◽  
C2c12 Myotubes ◽  
Chemical Components ◽  
Diabetic Mice ◽  
Cyclocarya Paliurus ◽  
Insulin Tolerance ◽  
Glucose Tolerance Tests ◽  
Glucose Stimulated Insulin Secretion ◽  
Hyperglycemic Effect

To identify the chemical components responsible for the anti-hyperglycemic effect of Cyclocarya paliurus (Batal.) Iljinsk (Juglandaceae) leaves, an ethanol extract (CPE) and a water extract (CPW) of C. paliurus leaves, as well as their total flavonoids (CPF), triterpenoids (CPT) and crude polysaccharides (CPP), were prepared and assessed on streptozotocin (STZ)-induced diabetic mice. After being orally administrated once a day for 24 days, CPF (300 mg/kg), CPP (180 mg/kg), or CPF+CPP (300 mg/kg CPF + 180 mg/kg CPP) treatment reversed STZ-induced body weight and muscle mass losses. The glucose tolerance tests and insulin tolerance tests suggested that CPF, CPP, and CPF+CPP showed anti-hyperglycemic effect in STZ-induced diabetic mice. Furthermore, CPF enhances glucose-stimulated insulin secretion in MIN6 cells and insulin-stimulated glucose uptake in C2C12 myotubes. CPF and CPP suppressed inflammatory cytokine levels in STZ-induced diabetic mice. Additionally, CPF and CPP improved STZ-induced diabetic nephropathy assessed by H&E staining, blood urea nitrogen content, and urine creatinine level. The molecular networking and Emperor analysis results indicated that CPF showed potential anti-hyperglycemic effects, and HPLC–MS/MS analysis indicated that CPF contains 3 phenolic acids and 9 flavonoids. In contrast, CPT (650 mg/kg) and CPC (300 mg/kg CPF + 180 mg/kg CPP + 650 mg/kg CPT) did not show anti-hyperglycemic effect. Taken together, polysaccharides and flavonoids are responsible for the anti-hyperglycemic effect of C. paliurus leaves, and the clinical application of C. paliurus need to be refined.

The Anti-Hyperglycemic Effect of Plants in Genus Gynura Cass.

2009 ◽  
Vol 37 (05) ◽  
pp. 961-966 ◽  
Author(s):  
Wei-Lin Li ◽  
Bing-Ru Ren ◽  
Min-Zhuo ◽  
Yong Hu ◽  
Chang-Gen Lu ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Ethyl Acetate ◽  
Glucose Level ◽  
Ethanol Extract ◽  
Diabetic Mice ◽  
Crude Drug ◽  
Pharmacological Tests ◽  
Ethanol Extracts ◽  
Hyperglycemic Effect

Gynura divaricata (L.) DC and G. bicolor DC are used as secret recipes to treat diabetes mellitus in some parts of China. Pharmacological tests were performed to prove the anti-hyperglycemic effect of these two plants of genus Gynura Cass. in this study. Both water and 95% ethanol extracts of fresh G. divaricata had significant effects on lowering blood glucose level in normal mice, in which the dose of 0.4 g (crude drug)/kg of 95% ethanol extract was more effective than 50 mg/kg glyburide. The ethyl acetate and n-butanol extracts of dried G. divaricata had significant effects on lowering blood glucose level in normal and alloxan diabetic mice too. Both ethyl acetate and n-butanol extracts of dried G. bicolor showed very significant effect on lowering blood glucose level to normal and alloxan-diabetic mice, and the dose 4.0 g (crude drug)/kg had a more hypoglycemic effect than 50 mg/kg glyburide in normal mice.

scholarly journals 3,4-Dicaffeoylquinic Acid, a Major Constituent of Brazilian Propolis, Increases TRAIL Expression and Extends the Lifetimes of Mice Infected with the Influenza A Virus

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Tomoaki Takemura ◽  
Tomohiko Urushisaki ◽  
Mayuko Fukuoka ◽  
Junji Hosokawa-Muto ◽  
Taketoshi Hata ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Water Extract ◽  
Ethanol Extract ◽  
Major Constituent ◽  
Chemical Components ◽  
Control Group ◽  
Dicaffeoylquinic Acid ◽  
Brazilian Green Propolis

Brazilian green propolis water extract (PWE) and its chemical components, caffeoylquinic acids, such as 3,4-dicaffeoylquinic acid (3,4-diCQA), act against the influenza A virus (IAV) without influencing the viral components. Here, we evaluated the anti-IAV activities of these compoundsin vivo. PWE or PEE (Brazilian green propolis ethanol extract) at a dose of 200 mg/kg was orally administered to Balb/c mice that had been inoculated with IAV strain A/WSN/33. The lifetimes of the PWE-treated mice were significantly extended compared to the untreated mice. Moreover, oral administration of 3,4-diCQA, a constituent of PWE, at a dose of 50 mg/kg had a stronger effect than PWE itself. We found that the amount of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) mRNA in the mice that were administered 3,4-diCQA was significantly increased compared to the control group, while H1N1 hemagglutinin (HA) mRNA was slightly decreased. These data indicate that PWE, PEE or 3,4-diCQA possesses a novel and unique mechanism of anti-influenza viral activity, that is, enhancing viral clearance by increasing TRAIL.

Pharmacological Studies on Anti-Hyperglycemic Effect of Folium Eriobotryae

2007 ◽  
Vol 35 (04) ◽  
pp. 705-711 ◽  
Author(s):  
Wei-Lin Li ◽  
Ju-Lan Wu ◽  
Bing-Ru Ren ◽  
Jian Chen ◽  
Chang-Gen Lu
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Ethanol Extract ◽  
Eriobotrya Japonica ◽  
Diabetic Mice ◽  
Crude Drug ◽  
Pharmacological Tests ◽  
Pharmacological Studies ◽  
Treatment Of Diabetes ◽  
Hyperglycemic Effect

Folium Eriobotryae, dried leaves of Eriobotrya japonica (Thunb.) Lindl. is a traditional Chinese medicine with rich resources in China. This research investigated the anti-hyperglycemic effect of Folium Eriobotryae on normal and alloxan-diabetic mice. The 70% ethanol extract of Folium Eriobotryae (EJA-0) in doses of 15, 30 and 60 g (crude drug)/kg exerted a significant hypoglycemic effect on alloxan-diabetic mice, among which 30 g/kg of EJA-0 was more effective than 100 mg/kg of phenformin. The total sesquiterpenes (EJA-1) 30 g (crude drug)/kg had significant effect on lowering blood glucose level in normal or/and alloxan-diabetic mice. The tests of maximum dosage and acute toxicity showed that EJA-1 was safe (MD = 360 g/kg, LD50 = 400.1 g/kg). The pharmacological tests on anti-hyperglycemic effects of EJA-0 and EJA-1 prove that Folium Eriobotryae is an outstanding material to develop medicine for treatment of diabetes mellitus.

scholarly journals Triterpenoids from Cyclocarya paliurus that Enhance Glucose Uptake in 3T3-L1 Adipocytes

Molecules ◽  
2019 ◽  
Vol 24 (1) ◽  
pp. 187 ◽  
Author(s):  
Zhu-Jun Fang ◽  
Sheng-Nan Shen ◽  
Jia-Min Wang ◽  
Yong-Jiang Wu ◽  
Chang-Xin Zhou ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Therapeutic Potential ◽  
Ethanol Extract ◽  
2D Nmr ◽  
C2c12 Myotubes ◽  
Spectroscopic Techniques ◽  
Cyclocarya Paliurus ◽  
Insulin Sensitizers ◽  
Triterpenoid Glycosides ◽  
Amp Activated Protein Kinase

Four previously undescribed compounds, including three rarely occurring seco-dammarane triterpenoid glycosides and a pentacyclic triterpenic acid, were isolated from a 70% ethanol extract of the leaves of Cyclocarya paliurus (Juglandaceae), along with eleven known triterpenoids. Their structures were determined by spectroscopic techniques, including 2D NMR and HRESIMS, as well as chemical methods. Among them, several triterpenoids enhanced insulin stimulated glucose uptake in both 3T3-L1 adipocytes and C2C12 myotubes. Furthermore, compound 1 dose-dependently increased glucose uptake through activating AMP-activated protein kinase (AMPK)-p38 pathway. Collectively, triterpenoids from C. paliurus could be developed as insulin sensitizers, which might have therapeutic potential for insulin resistance and hyperglycemia.

scholarly journals High-Throughput Identification of Organic Compounds from Polygoni Multiflori Radix Praeparata (Zhiheshouwu) by UHPLC-Q-Exactive Orbitrap-MS

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3977
Author(s):  
Shaoyun Wang ◽  
Xiaozhu Sun ◽  
Shuo An ◽  
Fang Sang ◽  
Yunli Zhao ◽  
...  
Keyword(s):  
High Performance ◽  
Chemical Constituents ◽  
Water Extract ◽  
Ethanol Extract ◽  
In Vivo Studies ◽  
Polygonum Multiflorum ◽  
Q Exactive ◽  
Orbitrap Ms

Polygoni Multiflori Radix Praeparata (PMRP), as the processed product of tuberous roots of Polygonum multiflorum Thunb., is one of the most famous traditional Chinese medicines, with a long history. However, in recent years, liver adverse reactions linked to PMRP have been frequently reported. Our work attempted to investigate the chemical constituents of PMRP for clinical research and safe medication. In this study, an effective and rapid method was established to separate and characterize the constituents in PMRP by combining ultra-high performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS). Based on the accurate mass measurements for molecular and characteristic fragment ions, a total of 103 compounds, including 24 anthraquinones, 21 stilbenes, 15 phenolic acids, 14 flavones, and 29 other compounds were identified or tentatively characterized. Forty-eight compounds were tentatively characterized from PMRP for the first time, and their fragmentation behaviors were summarized. There were 101 components in PMRP ethanol extract (PMRPE) and 91 components in PMRP water extract (PMRPW). Simultaneously, the peak areas of several potential xenobiotic components were compared in the detection, which showed that PMRPE has a higher content of anthraquinones and stilbenes. The obtained results can be used in pharmacological and toxicological research and provided useful information for further in vitro and in vivo studies.

Chemical components of the rhizomes of Drynaria fortunei (KUNZE) J. Sm. (polypodiaceae) in Vietnam

2011 ◽  
Vol 76 (9) ◽  
pp. 1133-1139 ◽  
Author(s):  
Pham Thi Nhat Trinh ◽  
Nguyen Cong Hao ◽  
Phan Thanh Thao ◽  
Le Tien Dung
Keyword(s):  
Data Analysis ◽  
Spectral Data ◽  
Phenolic Acid ◽  
Ethanol Extract ◽  
Chemical Components ◽  
Spectroscopic Methods ◽  
Phenylpropanoid Glycoside ◽  
Spectral Data Analysis ◽  
First Time

From the ethanol extract of Drynaria fortunei (KUNZE) J. Sm., a new phenylpropanoid glycoside, fortunamide (1), was isolated and characterized by spectroscopic methods. Together with a new glycoside, 9 known compounds, including three curcuminoids (2–4), two isoprenylated flavonoids (5, 6), two flavonoids (7, 8), one monoterpenoid (9) and one phenolic acid (10) were isolated and identified by spectral data analysis from the rhizomes of Drynaria fortunei (KUNZE) J. Sm. Eight of them were isolated from Drynaria fortunei (KUNZE) J. Sm. for the first time.

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