scholarly journals The First Selenoanhydride in the Series of Chlorophyll a Derivatives, Its Stability and Photoinduced Cytotoxicity

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7298
Author(s):  
Viktor Pogorilyy ◽  
Anna Plyutinskaya ◽  
Nikita Suvorov ◽  
Ekaterina Diachkova ◽  
Yuriy Vasil’ev ◽  
...  
Keyword(s):  
Spectral Properties ◽  
Quantum Yields ◽  
Chalcogen Atom ◽  
Target Compound ◽  
Ph Values ◽  
High Fluorescence ◽  
The Stability ◽  
First Time ◽  
Chlorin P6

In this work, we obtained the first selenium-containing chlorin with a chalcogen atom in exlocycle E. It was shown that the spectral properties were preserved in the target compound and the stability increased at two different pH values, in comparison with the starting purpurin-18. The derivatives have sufficiently high fluorescence and singlet oxygen quantum yields. The photoinduced cytotoxicity of sulfur- and selenium-anhydrides of chlorin p6 studied for the first time in vitro on the S37 cell line was found to be two times higher that of purpurin-18 and purpurinimide studied previously. Moreover, the dark cytotoxicity increased four-fold in comparison with the latter compounds. Apparently, the increase in the dark cytotoxicity is due to the interaction of the pigments studied with sulfur- and selenium-containing endogenous intracellular compounds. Intracellular distributions of thioanhydride and selenoanhydride chlorin p6 in S37 cells were shown in cytoplasm by diffusion distribution. The intracellular concentration of the sulfur derivative turned out to be higher and, as a consequence, its photoinduced cytotoxicity was higher as well.

scholarly journals Multiple Fungicide Resistance in Botrytis cinerea from Greenhouse Strawberries in Hubei Province, China

Plant Disease ◽  
2017 ◽  
Vol 101 (4) ◽  
pp. 601-606 ◽  
Author(s):  
F. Fan ◽  
M. S. Hamada ◽  
N. Li ◽  
G. Q. Li ◽  
C. X. Luo
Keyword(s):  
Botrytis Cinerea ◽  
Management Strategies ◽  
Hubei Province ◽  
Early Summer ◽  
Initial Generation ◽  
Succinate Dehydrogenase Subunit B ◽  
The Stability ◽  
First Time

Two hundred and forty isolates of Botrytis cinerea were collected during the early summer of 2012 and 2013 from strawberry greenhouses in 10 locations in Hubei Province and examined for sensitivity to five fungicides, most of which were commonly used to control this fungus. High frequency of resistance to carbendazim (Car, 63.63%) and cyprodinil (Cyp, 42.42%) was detected. Boscalid-resistant (BosR) isolates were detected for the first time in China, whereas no fludioxonil-resistant isolates were identified. Dual resistance to carbendazim and diethofencarb (Die) was also detected. There were six phenotypes of resistance profile (i.e., CarRDieSBosSCypS, CarRDieRBosSCypS, CarRDieSBosSCypR, CarRDieSBosRCypS, CarRDieRBosSCypR, and CarRDieSBosRCypR). CarRDieSBosSCypS and CarRDieSBosSCypR were the most common phenotypes, occurring at eight and seven locations, respectively. After 10 successive transfers on fungicide-free potato dextrose agar, tested resistant isolates retained levels of resistance similar to or comparative with the initial generation, indicating the stability of these resistances. Fitness evaluations based on investigation of mycelial growth, osmotic sensitivity, sporulation in vitro and in vivo, and virulence revealed the uncompromising fitness in resistant isolates, except that decreased virulence was observed in BosR isolates. The molecular basis of carbendazim, diethofencarb, and boscalid resistance was investigated. Results showed that all 13 sequenced carbendazim-resistant isolates harbored the mutation E198V or E198A in the β-tubulin gene and the five isolates with dual resistance to carbendazim and diethofencarb showed the mutation E198K in the same gene. BosR isolates possessed the H272R mutation in succinate dehydrogenase subunit B gene. The results achieved in this study challenge the current management strategies for B. cinerea, which largely depend on applications of these fungicides.

A Novel pH-Sensitive Microsphere Composed of CM-Chitosan and Alginate for Sulforaphane Delivery

2011 ◽  
Vol 687 ◽  
pp. 539-547 ◽  
Author(s):  
Hui Wang ◽  
Hao Liang ◽  
Qi Peng Yuan ◽  
Tian Xin Wang
Keyword(s):  
Sodium Sulfate ◽  
Anticancer Agent ◽  
Ph Sensitive ◽  
Water Soluble ◽  
Ph Values ◽  
Swelling Studies ◽  
The Stability ◽  
Carboxymethylated Chitosan

Sulforaphane (SF) has been proved to be an effective anticancer agent according to its experiments bothin vitroandin vivo. To date, there is few reported method to deliver SF for increasing its bioactivity and stability. In this study, a novel pH-sensitive microsphere composed of water-soluble carboxymethylated chitosan (CMCS) and alginate mixed with sodium sulfate was developed for SF delivery. Swelling studies and release characteristics under different pH values of microspheres were investigated. Then, the release of SF from test microspheres was studied in simulated gastric and segmented intestinal media. It has been found that the SF cumulated release in 5h was increased from 55.89% to 76.73% when the microspheres mixed with sodium sulfate. In addition, the stability of SF embedded in CMCS/alginate microspheres was also significantly improved. Under pH 7.4, free SF had a severe degradation of approximate 100% within 210 min, whereas the change of the SF in microspheres was only a decrease of about 10%. The results suggested that the microspheres of CMCS and alginate could be a suitable pH-sensitive carrier to increase the stability of SF in the segmented intestine.

scholarly journals Abnormally aggregation-induced emissions observed from hydrogen- and silyl-substituted siloles

2020 ◽  
Vol 43 (1) ◽  
pp. 125-131
Author(s):  
Zhengang Han ◽  
Kefeng Xie
Keyword(s):  
Hydrogen Atom ◽  
Theoretical Calculations ◽  
Quantum Yields ◽  
Aggregation Induced Emission ◽  
Fluorescence Quantum Yields ◽  
High Fluorescence ◽  
First Time

AbstractSilacyclopentadienes (siloles) are currently of great interest because of their intriguing aggregation-induced emission (AIE) characteristics. In this work, abnormally AIE phenomena were observed from silyl- and hydrogen-substituted siloles respectively. We propose silyl-substituent and hydrogen atom effects to explain this via both experiments and theoretical calculations. It was discovered that trimethylsilyl substituents destroyed the p-interactions with the silole core and resulted in the non-fluorescence. Additionally, 1-chloro-1-H-2,3,4,5-tetraphenylsilole featuring strong fluorescences in both solid and solution states with high fluorescence quantum yields represents a new potential photoelectric material. This work not only reports the silole with strong fluorescences in both solid and solution states for the first time but also contributs to enrich the AIE research of siloles.

Stable CsPbBr3 perovskite quantum dots with high fluorescence quantum yields

2018 ◽  
Vol 42 (12) ◽  
pp. 9496-9500 ◽  
Author(s):  
Min Zhang ◽  
Zhi-Quan Tian ◽  
Dong-Liang Zhu ◽  
He He ◽  
San-Wei Guo ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Quantum Yields ◽  
Fluorescence Quantum Yields ◽  
Perovskite Quantum Dots ◽  
High Fluorescence ◽  
The Stability

After replacing oleylamine with (3-aminopropyl)triethoxysilane, the stability of the as-prepared CsPbBr3 QDs was significantly improved.

Pyclen Based Ln(III) Complexes as Highly Luminescent Bioprobes for in Vitro and in Vivo 1P and 2P Bioimaging Applications

2020 ◽  
Author(s):  
Nadège Hamon ◽  
Amandine Roux ◽  
Maryline Beyler ◽  
Jean-Christophe Mulatier ◽  
Chantal Andraud ◽  
...  
Keyword(s):  
High Performance ◽  
Breast Cancer Cells ◽  
Positive Impact ◽  
Quantum Yields ◽  
Human Breast ◽  
Zebrafish Model ◽  
High Brightness ◽  
First Time

Two pyclen based lanthanide chelators, <b>L<sup>4b</sup> </b>and <b>L<sup>4c</sup>, </b>bearing two specific picolinate 2P antennas (tailor-made for each targeted metal) and one acetate arm arranged in a dissymmetrical manner, have been synthesized to form, with the already described ligand <b>L<sup>4a</sup>,</b> a complete family of lanthanide luminescent bioprobes: [Eu<b>L<sup>4a</sup></b>], [Sm<b>L<sup>4a</sup></b>], [Yb<b>L<sup>4b</sup></b>], [Tb<b>L<sup>4c</sup></b>] and [Dy<b>L<sup>4c</sup></b>]. Additionally, symmetrically arranged regioisomer <b>L<sup>4a’</sup> </b>was also synthesized as well as its [Eu<b>L<sup>4a’</sup></b>] complex to highlight the astonishing positive impact of the dissymmetrical <i>N</i>-distribution of the functional chelating arm. The investigation clearly shows the high performance of each bioprobe, which, depending on the complexed lanthanide, could be used in various applications. Each presents high brightness, quantum yields and lifetimes. Staining of the complexes into living human breast cancer cells was observed. In addition, <i>in vivo</i> 2P-microscopy was performed for the first time on a living Zebrafish model with [Eu<b>L<sup>4a</sup></b>]. No apparent toxicity was detected on the growth of the zebrafish and images of high quality were obtained.

scholarly journals In Vitro Study of MefenamateStarch as Drug Delivery System

2013 ◽  
Vol 10 (3) ◽  
pp. 954-964
Author(s):  
Baghdad Science Journal
Keyword(s):  
In Vitro Study ◽  
Controlled Drug Release ◽  
Gastric Fluid ◽  
Molar Ratio ◽  
Ph Values ◽  
Ft Ir ◽  
Uv Visible ◽  
The Stability ◽  
Hydrolysis Of

Mefenamic acid was esterified with starchwith[1:1] Molar ratio, as drug substituted with natural polymer, to prolongthe period of hydrolysis of drug polymer with other advantages. The new prodrug starch was characterized by FT-IR and UV-Visible and 1H-NMR spectroscopies. The physical properties were studied and controlled drug release was studied in different pH values at 37oC. The stability of drug was carried out by measuring the absorbance of mefenamic starch which hydrolyzed in HCl solution of pH 1.1 (artificial gastric fluid) and phosphate buffer of pH 7.4 (simulating intestinal fluid SIF) at 37oC for several days. The thermal analysis such as DSC was studied.

Pyclen Based Ln(III) Complexes as Highly Luminescent Bioprobes for in Vitro and in Vivo 1P and 2P Bioimaging Applications

2020 ◽  
Author(s):  
Nadège Hamon ◽  
Amandine Roux ◽  
Maryline Beyler ◽  
Jean-Christophe Mulatier ◽  
Chantal Andraud ◽  
...  
Keyword(s):  
High Performance ◽  
Breast Cancer Cells ◽  
Positive Impact ◽  
Quantum Yields ◽  
Human Breast ◽  
Zebrafish Model ◽  
High Brightness ◽  
First Time

Two pyclen based lanthanide chelators, <b>L<sup>4b</sup> </b>and <b>L<sup>4c</sup>, </b>bearing two specific picolinate 2P antennas (tailor-made for each targeted metal) and one acetate arm arranged in a dissymmetrical manner, have been synthesized to form, with the already described ligand <b>L<sup>4a</sup>,</b> a complete family of lanthanide luminescent bioprobes: [Eu<b>L<sup>4a</sup></b>], [Sm<b>L<sup>4a</sup></b>], [Yb<b>L<sup>4b</sup></b>], [Tb<b>L<sup>4c</sup></b>] and [Dy<b>L<sup>4c</sup></b>]. Additionally, symmetrically arranged regioisomer <b>L<sup>4a’</sup> </b>was also synthesized as well as its [Eu<b>L<sup>4a’</sup></b>] complex to highlight the astonishing positive impact of the dissymmetrical <i>N</i>-distribution of the functional chelating arm. The investigation clearly shows the high performance of each bioprobe, which, depending on the complexed lanthanide, could be used in various applications. Each presents high brightness, quantum yields and lifetimes. Staining of the complexes into living human breast cancer cells was observed. In addition, <i>in vivo</i> 2P-microscopy was performed for the first time on a living Zebrafish model with [Eu<b>L<sup>4a</sup></b>]. No apparent toxicity was detected on the growth of the zebrafish and images of high quality were obtained.

scholarly journals LncRNA CRNDE Promotes ATG4B-Mediated Autophagy and Alleviates the Sensitivity of Sorafenib in Hepatocellular Carcinoma Cells

2021 ◽  
Vol 9 ◽  
Author(s):  
Lingxi Chen ◽  
Liangbo Sun ◽  
Xufang Dai ◽  
Tao Li ◽  
Xiaojing Yan ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Colorectal Neoplasia ◽  
Promoting Effect ◽  
Non Coding Rna ◽  
Novel Target ◽  
The Stability ◽  
First Time ◽  
Hcc Cells

Autophagy is closely related to the growth and drug resistance of cancer cells, and autophagy related 4B (ATG4B) performs a crucial role in the process of autophagy. The long non-coding RNA (lncRNA) colorectal neoplasia differentially expressed (CRNDE) promotes the progression of hepatocellular carcinoma (HCC), but it is unclear whether the tumor-promoting effect of CRNDE is associated with the regulation of ATG4B and autophagy. Herein, we for the first time demonstrated that CRNDE triggered autophagy via upregulating ATG4B in HCC cells. Mechanistically, CRNDE enhanced the stability of ATG4B mRNA by sequestrating miR-543, leading to the elevation of ATG4B and autophagy in HCC cells. Moreover, sorafenib induced CRNDE and ATG4B as well as autophagy in HCC cells. Knockdown of CRNDE sensitized HCC cells to sorafenib in vitro and in vivo. Collectively, these results reveal that CRNDE drives ATG4B-mediated autophagy, which attenuates the sensitivity of sorafenib in HCC cells, suggesting that the pathway CRNDE/ATG4B/autophagy may be a novel target to develop sensitizing measures of sorafenib in HCC treatment.

scholarly journals Study of stability of proline-containing derivatives of dopamine and serotonin in the biological media in vitro experiments

2019 ◽  
Vol 65 (6) ◽  
pp. 498-506 ◽  
Author(s):  
K.V. Shevchenko ◽  
L.A. Andreeva ◽  
I.Yu. Nagaev ◽  
V.P. Shevchenko ◽  
N.F. Myasoedov
Keyword(s):  
Leucine Aminopeptidase ◽  
Biologically Active ◽  
Carboxypeptidase Y ◽  
Vital Activity ◽  
Prolonged Action ◽  
Carboxypeptidase B ◽  
The Stability ◽  
First Time ◽  
Derivatives Of

Boc-Gly-Pro-DP, Z-Gly-Pro-DP, LA-Gly-Pro-DP, Boc-Gly-Pro-Srt, Z-Gly-Pro-Srt were synthesized for the first time. The stability of these compounds in the presence of leucine aminopeptidase, carboxypeptidase Y, carboxypeptidase B and proline endopeptidase (PEP) was determined. It turned out that the compounds are stable in the presence of aminopeptidases and carboxypeptidases. In the presence of PEP, dopamine (DP) and serotonin (Srt) are cleaved from the synthesized preparations. Thus, new proline-containing Srt and DP derivatives were obtained, Srt and DP could be gradually released from them. This suggest the possibility of a prolonged action of these biologically active compounds on the vital activity of cells and, consequently, of the whole organism.

scholarly journals Spectral Properties of Dyes with Interfragmental Charge Transfer: Solvatochromism and Solvatofluorochromism of 2-(3-Coumaroyl)-benzopyrylium Perchlorates

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Edward V. Sanin ◽  
Alexander I. Novikov ◽  
Alexander D. Roshal
Keyword(s):  
Charge Transfer ◽  
Spectral Properties ◽  
Spectral Characteristics ◽  
Quantum Yields ◽  
Absorption Bands ◽  
Long Wavelength ◽  
Solvent Parameters ◽  
Wavelength Absorption ◽  
Solvent Molecules ◽  
The Stability

The paper deals with the spectral properties of dyes with interfragmental charge transfer, in particular, derivatives of 2-(3-coumaroyl)benzopyrylium (CBP) perchlorates. The positions of long-wavelength absorption and emission bands, quantum yields, and lifetimes of fluorescence were measured, mostly in aprotic solvents. Regression analysis of the relationship between the spectral characteristics of CBP and the main solvent parameters (polarity, polarisability, nucleophilicity, and electrophilicity) was carried out. It was found that the characteristics of CBP depend mainly on two solvent parameters—polarity and/or nucleophilicity. An increase in these parameters results in a hypsochromic shift of absorption bands and a decrease of lifetimes and fluorescence intensity. The positions of the emission bands can demonstrate either hypsochromism or bathochromism, depending on the nature of the substituents. The solvatofluorochromic effects are not as strong as the solvatochromic ones. We believe that the spectral behaviour of CBP can be explained by cation depolarisation and by a decrease in the stability of nucleophilic complexes with solvent molecules that is associated with interfragmental charge transfer following excitation, relaxation, and radiative deactivation of the excited state.

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