scholarly journals Synthesis of Carbon Quantum Dot Nanoparticles Derived from Byproducts in Bio-Refinery Process for Cell Imaging and In Vivo Bioimaging

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 387 ◽  
Author(s):  
Caoxing Huang ◽  
Huiling Dong ◽  
Yan Su ◽  
Yan Wu ◽  
Robert Narron ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Cell Imaging ◽  
Degradation Products ◽  
Green Fluorescence ◽  
One Pot ◽  
Carbon Nanoparticle ◽  
Carbon Quantum Dot ◽  
Potential Applications ◽  
Main Components

The carbon quantum dot (CQD), a fluorescent carbon nanoparticle, has attracted considerable interest due to its photoluminescent property and promising applications in cell imaging and bioimaging. In this work, biocompatible, photostable, and sustainably sourced CQDs were synthesized from byproducts derived from a biorefinery process using one-pot hydrothermal treatment. The main components of byproducts were the degradation products (autohydrolyzate) of biomass pretreated by autohydrolysis. The as-synthesized CQDs had a size distribution from 2.0–6.0 nm and had high percentage of sp2 and sp3 carbon groups. The CQDs showed blue-green fluorescence with a quantum yield of ~13%, and the fluorescence behaviors were found to be stable with strong resistance to photobleaching and temperature change. In addition, it is found that the as-synthesized CQDs could be used for imaging of cells and tumors, which show potential applications in bioimaging and related fields such as phototherapy and imaging.

scholarly journals Monitoring of Enzymatic Proteolysis Using Self-Assembled Quantum Dot-Protein Substrate Sensors

2008 ◽  
Vol 2008 ◽  
pp. 1-10 ◽  
Author(s):  
Aaron R. Clapp ◽  
Ellen R. Goldman ◽  
H. Tetsuo Uyeda ◽  
Eddie L. Chang ◽  
Jessica L. Whitley ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Proteolytic Enzymes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Enzymatic Digestion ◽  
Enzymatic Proteolysis ◽  
Potential Applications ◽  
Multiple Copies ◽  
Self Assembled

We have previously utilized hybrid semiconductor quantum dot- (QD-) peptide substrates for monitoring of enzymatic proteolysis. In this report, we expand on this sensing strategy to further monitor protein-protease interactions. We utilize QDs self-assembled with multiple copies of dye-labeled proteins as substrates for the sensing of protease activity. Detection of proteolysis is based on changes in the rate of fluorescence resonance energy transfer (FRET) between the QDs and the proximal dye-labeled proteins following protein digestion by added enzyme. Our study focused on two representative proteolytic enzymes: the cysteine protease papain and the serine protease endoproteinase K. Analysis of the enzymatic digestion allowed us to estimate minimal values for the enzymatic activities of each enzyme used. Mechanisms of enzymatic inhibition were also inferred from the FRET data collected in the presence of inhibitors. Potential applications of this technology include drug discovery assays and in vivo cellular monitoring of enzymatic activity.

scholarly journals A novel AIE-active dye for fluorescent nanoparticles by one-pot combination of Hantzsch reaction and RAFT polymerization: synthesis, molecular structure and application in cell imaging

RSC Advances ◽  
2019 ◽  
Vol 9 (56) ◽  
pp. 32601-32607 ◽  
Author(s):  
Yali Chen ◽  
Zengfang Huang ◽  
Xiaobo Liu ◽  
Liucheng Mao ◽  
Jinying Yuan ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Cell Imaging ◽  
Raft Polymerization ◽  
Fluorescent Nanoparticles ◽  
Hantzsch Reaction ◽  
One Pot ◽  
Fluorescent Polymers ◽  
Potential Applications

This work reported the fabrication of a novel AIE-active dye and its amphiphilic PEG-TPD fluorescent polymers via one-pot combination of RAFT polymerization and Hantzsch reaction, which showed potential applications in bioimaging.

Developing Electropositive Citric Acid-Polyethylenimine Carbon Quantum Dot with High Biocompatibility and Labeling Performance for Mesenchymal Stem Cells in Vitro and in Vivo

2022 ◽  
Author(s):  
Bo Jiang ◽  
Hui Yang ◽  
Ying Guo ◽  
Cong Liu ◽  
Hua Song ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Citric Acid ◽  
Quantum Dot ◽  
Carbon Quantum Dot

Mesenchymal stem cells (MSCs) have been widely adopted to cure many types of diseases and how they migrate to the damaged tissues is still not clear. Thus, it is significant...

Simultaneous near-infrared and green fluorescence from single conjugated polymer dots with aggregation-induced emission fluorogen for cell imaging

2018 ◽  
Vol 6 (47) ◽  
pp. 7871-7876 ◽  
Author(s):  
Sitong Chen ◽  
Shuang Cui ◽  
Rongxin Du ◽  
Ming Liu ◽  
Wei-Kai Tsai ◽  
...  
Keyword(s):  
Conjugated Polymer ◽  
Near Infrared ◽  
Cell Imaging ◽  
Green Fluorescence ◽  
Aggregation Induced Emission ◽  
Polymer Dots

Highly fluorescent conjugated polymer dots with aggregation-induced emission fluorogen were applied in in vitro and in vivo cell imaging.

Facile green and one-pot synthesis of purple perilla derived carbon quantum dot as a fluorescent sensor for silver ion

Talanta ◽  
2019 ◽  
Vol 201 ◽  
pp. 1-8 ◽  
Author(s):  
Xinyi Zhao ◽  
Sen Liao ◽  
Lumin Wang ◽  
Qi Liu ◽  
Xiaoqing Chen
Keyword(s):  
Quantum Dot ◽  
Fluorescent Sensor ◽  
Silver Ion ◽  
One Pot ◽  
Carbon Quantum Dot ◽  
One Pot Synthesis
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