scholarly journals Dietary Supplements of Shiikuwasha Extract Attenuates Osteoarthritis Progression in Meniscal/ligamentous Injury and Obese Rats

Nutrients ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1312 ◽  
Author(s):  
Yu-Wen Yen ◽  
Ying-Jiun Lai ◽  
Zwe-Ling Kong
Keyword(s):  
Cruciate Ligament ◽  
Density Lipoprotein ◽  
Cartilage Degeneration ◽  
Degenerative Joint Disease ◽  
Janus Kinase ◽  
Joint Disease ◽  
Sprague Dawley ◽  
Bioactive Substances ◽  
Necrosis Factor Alpha ◽  
Anterior Cruciate

Osteoarthritis (OA), also called degenerative joint disease, is characterized by joint cartilage loss and is strongly linked to obesity. Medicine to alleviate pain is currently the only treatment. Shiikuwasha extract (SE) has been reported to possess valuable bioactive substances exhibiting anti-inflammatory, antiobesity, and anticancer effects. Research is limited to the use of SE in the treatment of OA and obesity. We performed both anterior cruciate ligament transections and medial meniscectomies to induce OA on Sprague–Dawley rats after 11 weeks of a high fat diet followed by 9 weeks of oral SE administration (300, 600, and 1500 mg/kg). This study showed that SE treatment could reduce weight gain and joint pain. Additionally, SE significantly decreased triglycerides and total cholesterol in plasma of the S1500 group but increased high-density lipoprotein cholesterol in the plasma of the S600 group. Meanwhile, plasma levels of tumor necrosis factor alpha (TNF-α) was significantly reduced in the S1500 groups. Histopathological findings confirmed administration of SE attenuated cartilage degeneration. Immunohistochemistry examination demonstrated that caspase 3 and phospho-Janus kinase 2 (p-JAK2) expression levels on chondrocytes were downregulated by SE treatment. Our findings demonstrate that SE can alleviate OA progression by improving obesity.

Neurogenic origin of articular hyperemia in early degenerative joint disease

1999 ◽  
Vol 276 (3) ◽  
pp. R745-R752 ◽  
Author(s):  
Jason J. McDougall ◽  
William R. Ferrell ◽  
Robert C. Bray
Keyword(s):  
Joint Instability ◽  
Cruciate Ligament ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Calcitonin Gene ◽  
Neurogenic Origin ◽  
Vasomotor Activity ◽  
Anterior Cruciate ◽  
Supporting Structures ◽  
Acl Transection

It has been speculated that joint instability resulting from anterior cruciate ligament (ACL) rupture could be exacerbated by changes in vasomotor activity in the remaining supporting structures. In this study, the effect of ACL transection on medial collateral ligament (MCL) basal perfusion and its responsiveness to calcitonin gene-related peptide (CGRP) and sympathetic adrenergic influences was examined. Using urethan-anesthetized rabbits, we tested the effects of CGRP and its antagonist CGRP-(8—37) by topical application of these agents to the exposed knee while sympathetic influences were tested by electrically stimulating the saphenous nerve. It was found that MCL basal perfusion was elevated in ACL-sectioned joints; however, this effect was abrogated by prior resection of the articular nerve supply. At the doses tested, the normal vasodilator response to CGRP was abolished in ACL-sectioned joints, whereas the response to CGRP-(8—37) was attenuated. Even under the influence of increased constrictor tone, MCL and capsule blood vessels still showed substantially reduced responses to exogenous CGRP administration. By contrast, nerve-mediated constrictor responses were mostly unaffected by joint instability. This study suggests that posttraumatic knee joint hyperemia is neurogenically mediated, possibly by increased secretion of CGRP.

Dynamic Compression in the Presence of TNF-Alpha Differentially Effects Gene Expression in Tibial Plateau Cartilage Covered and Uncovered by the Meniscus

2008 ◽  
Author(s):  
Scott L. Bevill ◽  
Thomas P. Andriacchi
Keyword(s):  
Tibial Plateau ◽  
Dynamic Compression ◽  
Cruciate Ligament ◽  
Cartilage Degeneration ◽  
Joint Loading ◽  
Necrosis Factor Alpha ◽  
Joint Injury ◽  
Factor Alpha ◽  
Anterior Cruciate ◽  
Necrosis Factor

It has been suggested that altered joint loading may be a cause of the cartilage degeneration commonly observed following joint destabilizing events such as anterior cruciate ligament (ACL) injury [1]. Changes in the biochemical environment of the joint accompany these changes in joint loading, though, with acute and chronic increases in synovial concentrations of proinflammatory cytokines such as tumor necrosis factor-alpha (TNFα) [2, 3]. TNFα is a potent catabolic factor associated with increased expression [4] and synthesis [5] of matrix proteases in articular cartilage, and therefore may play in important role in the degenerative events following joint injury.

scholarly journals Acupuncture reduces pain in rats with osteoarthritis by inhibiting MCP2/CCR2 signaling pathway

2020 ◽  
Vol 245 (18) ◽  
pp. 1722-1731
Author(s):  
Bocun Li ◽  
Li Jing ◽  
Li Jia ◽  
Tan Qian ◽  
Chen Jianyi ◽  
...  
Keyword(s):  
Protective Effect ◽  
Cruciate Ligament ◽  
Alternative Therapy ◽  
Cartilage Degeneration ◽  
Underlying Mechanism ◽  
Sprague Dawley ◽  
Nerve Sprouting ◽  
Anterior Cruciate ◽  
The Right ◽  
And Cartilage

Acupuncture is an emerging alternative therapy that has been beneficial for the pain of osteoarthritis (OA). However, the underlying mechanism of protective effect remains unclear. MCP1/CCR2 axis can be stimulated in various periods of OA, and we hypothesize that acupuncture may treat OA by regulating the MCP1/CCR2 axis. This study aimed to explore the effect of acupuncture at points ST35 and ST36 on the effects of hyperalgesia and cartilage in OA rats including the expression of chemokines, nerve growth factor (NGF), and inflammatory-related proteins. OA was induced in male Sprague–Dawley rats by anterior cruciate ligament transection at the right knee. The first acupuncture intervention was performed on the seventh day after surgery and once a day for seven weeks. The knee-pain-related behaviors, histology, and related protein were examined in this study. We have found that electroacupuncture at ST35 and ST36 can significantly alleviate the hyperalgesia and cartilage degeneration as well as reducing nerve sprouting in OA knee joint. Moreover, acupuncture treatment may inhibit the MCP1/CCR2 axis as well as down-regulate inflaming factor and NGF in cartilage and synovial tissue. The data presented here indicate that acupuncture exerts a protective effect against hyperalgesia and cartilage degeneration, and the mechanism might involve in chemokines and NGF pathway.

scholarly journals Comparison of early-stage changes of osteoarthritis in cartilage and subchondral bone between two different rat models

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8934 ◽  
Author(s):  
Yutao Yang ◽  
Peiran Li ◽  
Songsong Zhu ◽  
Ruiye Bi
Keyword(s):  
Subchondral Bone ◽  
Early Stage ◽  
Cruciate Ligament ◽  
Cartilage Damage ◽  
Cartilage Degeneration ◽  
The Elderly ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Bone Change ◽  
Subchondral Bone Change

Osteoarthritis (OA) is a chronic degenerative joint disease and the major cause of joint pain and disability in the elderly. It is mainly characterized by articular cartilage degradation and subchondral bone remodeling. There are two main types of OA: natural occurring OA and secondary OA, mainly associated with aging and trauma, respectively. In this study, we established two OA models in rat knee joints to simulate the two types of OA, using the type II collagenase injection (CI) and anterior cruciate ligament transection (ACLT), respectively. After intervention for 2–6 weeks, cartilage and subchondral bone changes were detected in histological staining, immunochemistry, and micro-CT. Results showed that both models with typical pathology changes of OA were successfully induced, while the development and severity of OA process in the models were different. In ACLT rats, the cartilage damage was milder, lasted for a shorter time, and subchondral bone reconstruction occurred earlier, compared with the changes in CI rats. The cartilage damage was secondary to subchondral bone change in ACLT rats, while subchondral bone change was secondary to cartilage degeneration in CI rats. In conclusion, the interaction between cartilage and subchondral bone is different between the natural-occurring and secondary OA models. These two models not only suggest potential different mechanisms of the two types of OA, but also provide new directions for OA treatment and prevention.

Will the Cranial Cruciate Ligament-Deficient Caprine Stifle Joint Develop Degenerative Joint Disease?

1994 ◽  
Vol 07 (01) ◽  
pp. 14-17
Author(s):  
L. A. Thomson ◽  
J. E. F. Houlton ◽  
N. Rushton ◽  
M.J. Allen
Keyword(s):  
Cruciate Ligament ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Long Term Effects ◽  
Cranial Cruciate Ligament ◽  
Anterior Cruciate Ligament Deficiency ◽  
Stifle Joint ◽  
Anterior Cruciate ◽  
Meniscal Damage

SummaryUnilateral cranial cruciate ligament (CCL) resection was performed in six goats. Controls for this procedure included the contralateral (non-operated) joints and six normal joints. All CCL-deficient joints had a positive cranial drawer movement throughout the study, whereas all other joints were stable.None of the joints showed gross evidence of degenerative joint disease at necropsy 52 weeks after the operation. In addition, there were no statistically significant differences between either the frequency or severity of radiographic abnormalities in the two groups of joints.Despite long-term joint instability, degenerative joint disease did not develop in the CCL-deficient caprine stifle joint. When the goat is used as a model for anterior cruciate ligament-deficiency in man, the significance of any results should be assessed in the light of these findings.The long-term effects of experi-mentally induced cranial cruciate ligament (CCL) deficiency were studied in goats. All CCL-deficient joints had a positive cranial drawer movement, both immediately after surgery and at the end of the 52 week study. However, there was no evidence of cartilage or meniscal damage at postmortem examination, and stifle radiographs did not reveal evidence of degenerative joint disease.

scholarly journals HDAC2 interacts with microRNA-503-5p to regulate SGK1 in osteoarthritis

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Zheng Wang ◽  
Nan Zhou ◽  
Wengang Wang ◽  
Yangke Yu ◽  
Lei Xia ◽  
...  
Keyword(s):  
Histone Deacetylase ◽  
Cruciate Ligament ◽  
Cartilage Degeneration ◽  
Joint Disease ◽  
Luciferase Assay ◽  
Altered Expression ◽  
Histone Deacetylase 2 ◽  
Elevated Expression ◽  
Associated Proteins ◽  
Anterior Cruciate

Abstract Background Osteoarthritis (OA) is a disabling joint disease that causes articular cartilage degeneration. It has been implicated that altered expression of histone deacetylase 2 (HDAC2) is found in patients with OA. However, the specific role of HDAC2 in the development of OA still remains enigmatic. Hence, we sought to characterize the functional relevance of HDAC2 in the development of OA. Methods Anterior cruciate ligament surgery was performed to generate the rat model of OA. Luciferase assay was performed to evaluate the relationship between microRNA-503-5p (miR-503-5p) and serum- and glucocorticoid-inducible kinase-1 (SGK1). Functional experiments were conducted to examine the functional significance of miR-503-5p, histone deacetylase 2 (HDAC2), and SGK1 on the progression of OA by determining proliferation, apoptosis, and expression of apoptosis-associated proteins and inflammatory cytokines. Results HDAC2 could inhibit miR-503-5p expression. SGK1 was the target gene of miR-503-5p. Upregulation of miR-503-5p or silencing of HDAC2 contributed to enhanced proliferation, suppressed apoptosis (reduced expression of Caspase-3 and Bax but elevated expression of Bcl2), and promoted inflammation in chondrocytes of OA rats. Conclusion In conclusion, our study demonstrated that HDAC2 could promote OA through miR-503-5p/SGK1 axis, which might function as a therapeutic target for OA treatment.

Maltol inhibits the progression of osteoarthritis via the nuclear factor-erythroid 2-related factor-2/heme oxygenase-1 signal pathway in vitro and in vivo

2021 ◽  
Author(s):  
Ding-Chao Zhu ◽  
Yi-Han Wang ◽  
Jia-Hao Lin ◽  
Zhi-Min Miao ◽  
Jia-Jing Xu ◽  
...  
Keyword(s):  
Cartilage Degeneration ◽  
Degenerative Joint Disease ◽  
Signal Pathway ◽  
Joint Disease ◽  
Heme Oxygenase 1 ◽  
Related Factor ◽  
Nuclear Factor ◽  
Articular Cartilage Degeneration

Osteoarthritis (OA) is a common degenerative joint disease characterized by articular cartilage degeneration and inflammation. Currently, there is hardly any effective treatment for OA due to its complicated pathology and...

scholarly journals Treadmill Exercise after Controlled Abnormal Joint Movement Inhibits Cartilage Degeneration and Synovitis

Life ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 303
Author(s):  
Yuichiro Oka ◽  
Kenij Murata ◽  
Kaichi Ozone ◽  
Takuma Kano ◽  
Yuki Minegishi ◽  
...  
Keyword(s):  
Treadmill Exercise ◽  
Cruciate Ligament ◽  
Cartilage Degeneration ◽  
Research Society ◽  
Therapeutic Effects ◽  
Joint Movement ◽  
Knee Oa ◽  
Anterior Cruciate ◽  
Safranin O ◽  
Walking Group

Cartilage degeneration is the main pathological component of knee osteoarthritis (OA), but no effective treatment for its control exists. Although exercise can inhibit OA, the abnormal joint movement with knee OA must be managed to perform exercise. Our aims were to determine how controlling abnormal joint movement and treadmill exercise can suppress cartilage degeneration, to analyze the tissues surrounding articular cartilage, and to clarify the effect of treatment. Twelve-week-old ICR mice (n = 24) underwent anterior cruciate ligament transection (ACL-T) surgery on their right knees and were divided into three groups as follows: ACL-T, animals in the walking group subjected to ACL-T; controlled abnormal joint movement (CAJM), and CAJM with exercise (CAJM + Ex) (n = 8/group). Walking-group animals were subjected to treadmill exercise 6 weeks after surgery, including walking for 18 m/min, 30 min/day, 3 days/week for 8 weeks. Safranin-O staining, hematoxylin-eosin staining, and immunohistochemical staining were performed. The OARSI (Osteoarthritis research Society international) score was lower in the CAJM group than in the ACL-T group and was even lower in the CAJM + Ex group. The CAJM group had a lower meniscal injury score than the ACL-T group, and the CAJM + Ex group demonstrated a less severe synovitis than the ACL-T and CAJM groups. The observed difference in the perichondrium tissue damage score depending on the intervention method suggests different therapeutic effects, that normalizing joint motion can solve local problems in the knee joint, and that the anti-inflammatory effect of treadmill exercise can suppress cartilage degeneration.

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