scholarly journals Seroprevalence and Epidemiology of Toxoplasma gondii in Animals in the Qinghai-Tibetan Plateau Area, China

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 432
Author(s):  
Guojing Li ◽  
Wangli Zheng ◽  
Jinfang Yang ◽  
Tongsheng Qi ◽  
Yongcai He ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Tibetan Plateau ◽  
Animal Species ◽  
Elisa Test ◽  
Ovis Aries ◽  
Control Measures ◽  
Worldwide Distribution ◽  
Plateau Area ◽  
Tibetan Sheep ◽  
Wide Range

Toxoplasma gondii belongs to the Apicomplexan protozoa—an obligate intracellular parasite—causing toxoplasmosis that has a worldwide distribution and is very harmful to both human health and the livestock industry. However, the information on toxoplasmosis in the Qinghai-Tibetan Plateau Area (QTPA) and the seroprevalence of T. gondii in the food-borne animals in that area has been limited. Therefore, this study focused to T. gondii and toxoplasmosis to perform an indirect ELISA test based on recombinant TgSAG2 protein to establish a comprehensive record of the seroprevalence of T. gondii infections in a wide range of animals, including Tibetan sheep (Ovis aries), yaks (Bos grunniens), cows, chicken, pigs, and horses, in the QTPA. Overall, the seropositive rates of the specific-T. gondii IgG and IgM antibodies in all investigated animals were 44.1% (1179/2673) and 18.0% (469/2612), respectively. The 14.9% (389/2612) sera were determined to be both IgG and IgM positive samples, 30.2% (789/2673) were single-IgG seropositive, and a total of 80 in 2612 animals (3.0%) were single-IgM seropositive. Moreover, for the animal species, the pig was the most prevalent animal (90.2%, 304/337) for IgG positivity, followed by Tibetan sheep (50.7%, 460/907), chickens (45.8%, 229/500), yaks (21.1%, 140/663), cows (18.5%, 38/205) and horses (13.1%, 8/61), respectively. For the IgM antibody positivity, the pig was also the most prevalent animal (41.8%, 141/337), followed by Tibetan sheep (21.2%, 191/907), cows (15.1%, 31/205), chickens (12.4%, 62/500) and yaks (6.6%, 44/663), respectively. The significant differences in the prevalent distribution of T. gondii were found in the different altitudes. In conclusion, this study found the high seroprevalence for T. gondii infections among these animal species in the QTPA, and provides new data to facilitate further research for development of control measures against T. gondii infections in the surveyed locations.

scholarly journals The First Case of Diarrhoea in Tibetan Sheep, Ovis aries, Caused by Balantidium coli in the Qinghai Tibetan Plateau Area, China

2018 ◽  
Vol 56 (6) ◽  
pp. 603-607 ◽  
Author(s):  
Ying-Na Jian ◽  
Ge-Ping Wang ◽  
Xiu-Ping Li ◽  
Xue-Yong Zhang ◽  
Li-Qing Ma
Keyword(s):  
Tibetan Plateau ◽  
Ovis Aries ◽  
Balantidium Coli ◽  
First Case ◽  
Plateau Area ◽  
Tibetan Sheep

scholarly journals Phylogeography and Phylogenetic Evolution in Tibetan Sheep Based on MT-CYB Sequences

Animals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1177
Author(s):  
Jianbin Liu ◽  
Zengkui Lu ◽  
Chao Yuan ◽  
Fan Wang ◽  
Bohui Yang
Keyword(s):  
Mitochondrial Dna ◽  
Tibetan Plateau ◽  
Clustering Analysis ◽  
Genetic Resource ◽  
Population Studies ◽  
Ovis Aries ◽  
Base Pairs ◽  
Branching Order ◽  
Tibetan Sheep ◽  
Phylogenetic Evolution

To date, molecular genetics and population studies in Tibetan sheep (Ovis aries) have been limited, and little is known about the phylogenetic evolution and phylogeography of Tibetan sheep populations. The aim of the present research was to explore phylogeography and phylogenetic evolution of Tibetan sheep populations, on the basis of mitochondrial DNA (mtDNA) gene MT-CYB (1140 base pairs). Our dataset consisted of 641 MT-CYB sequences from the same amount of animals belonging to 15 populations of Tibetan sheep living in the Qinghai–Tibetan Plateau, China. Haplotype and nucleotide diversities were 0.748 ± 0.010 and 0.003 ± 0.001, respectively. The analysis of phylogeography revealed the presence of two formerly described haplogroups in 15 populations of Tibetan sheep, however only one haplogroup was present in Awang sheep. Moreover, 641 Tibetan sheep were distributed into a minimum of two clusters by clustering analysis. The 15 Tibetan sheep populations and 19 reference populations of 878 individuals were separated into six main groups based on their substitutions per site, from which we constructed a phylogenetic tree. Minor differences in branching order of various taxa between trees acquired from either gene were observed. This study provides insights on the origins and phylogenetic evolution of populations residing in the Qinghai–Tibetan Plateau, which will aid information of future conservation programs aimed at conserving this valuable genetic resource.

Rumen bacterial diversity of Tibetan sheep (Ovis aries) associated with different forage types on the Qinghai-Tibetan Plateau

2019 ◽  
Vol 65 (12) ◽  
pp. 859-869 ◽  
Author(s):  
Xiongxiong Cui ◽  
Zhaofeng Wang ◽  
Tianhai Yan ◽  
Shenghua Chang ◽  
Hong Wang ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Bacterial Community ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Diet Group ◽  
Ovis Aries ◽  
Bacterial Composition ◽  
Rumen Microbiome ◽  
Tibetan Sheep ◽  
Native Pasture

Diet is the great determinant of bacterial composition in the rumen. However, little is known about the rumen bacterial community of Tibetan sheep living in the special ecological environment of the Qinghai-Tibetan Plateau (QTP) of China. In the present study, we used high-throughput sequencing to investigate the rumen bacterial community of Tibetan sheep associated with two primary diets: alpine pasture diet (a continuation of the sheep’s natural grazing diet) and oat (Avena sativa) hay diet on the QTP. The results showed that bacterial community richness and species diversity of the oat hay diet group were significantly greater than that of the native pasture diet group (p < 0.05). Principal co-ordinate analysis and analysis of similarities revealed that the bacterial community of the oat hay diet group was distinctly different from that of the native pasture diet group (p < 0.05). Bacteroidetes and Firmicutes were the predominant microbial phyla in the rumen. The rumen of oat-hay-fed sheep had higher proportions of Proteobacteria and novel bacteria species than the rumen of native-pasture-fed sheep. Actinobacteria, an uncommon bacterial phylum, occurred only in the oat-hay-fed group. At the genus level, Komagataeibacter, Ruminococcaceae_UCG-014, and Ruminococcaceae_NK4A214 showed significantly higher relative abundance in the oat-hay-fed sheep than in the native-pasture-fed sheep (p < 0.05). This study is the first of the QTP to employ high-throughput sequencing to examine the influence of diet on the rumen microbiome of Tibetan sheep.

scholarly journals Descriptive Comparison of ELISAs for the Detection of Toxoplasma gondii Antibodies in Animals: A Systematic Review

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 605
Author(s):  
K. L. D. Tharaka D. Liyanage ◽  
Anke Wiethoelter ◽  
Jasmin Hufschmid ◽  
Abdul Jabbar
Keyword(s):  
Systematic Review ◽  
Toxoplasma Gondii ◽  
Animal Species ◽  
Specific Binding ◽  
Protein A ◽  
Cross Reactivity ◽  
Meat Juice ◽  
Wide Range ◽  
Eimeria Spp ◽  
Species Specific

Toxoplasma gondii is the zoonotic parasite responsible for toxoplasmosis in warm-blooded vertebrates. This systematic review compares and evaluates the available knowledge on enzyme-linked immunosorbent assays (ELISAs), their components, and performance in detecting T. gondii antibodies in animals. Four databases were searched for published scientific studies on T. gondii and ELISA, and 57 articles were included. Overall, indirect (95%) and in-house (67%) ELISAs were the most used types of test among the studies examined, but the ‘ID Screen® Toxoplasmosis Indirect Multi-species’ was common among commercially available tests. Varying diagnostic performance (sensitivity and specificity) and Kappa agreements were observed depending on the type of sample (serum, meat juice, milk), antigen (native, recombinant, chimeric) and antibody-binding reagents used. Combinations of recombinant and chimeric antigens resulted in better performance than native or single recombinant antigens. Protein A/G appeared to be useful in detecting IgG antibodies in a wide range of animal species due to its non-species-specific binding. One study reported cross-reactivity, with Hammondia hammondi and Eimeria spp. This is the first systematic review to descriptively compare ELISAs for the detection of T. gondii antibodies across different animal species.

scholarly journals Combination satellite and in-situ data for the determination of evapotranspiration over heterogeneous landscape of the Tibetan Plateau

2013 ◽  
Vol 13 (3) ◽  
pp. 8435-8453
Author(s):  
Y. Ma ◽  
Z. Zhu ◽  
L. Zhong ◽  
B. Wang ◽  
C. Han ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Land Surface ◽  
The Tibetan Plateau ◽  
Modis Data ◽  
In Situ Data ◽  
Heterogeneous Landscape ◽  
Plateau Area ◽  
Wide Range ◽  
Avhrr Data

Abstract. In this study, a new parameterization method based on MODIS (Moderate Resolution Imaging Spectroradiometer) data, AVHRR (Advanced Very High-Resolution Radiometer) data and in-situ data is constructed and tested for deriving the regional evaporative fraction (EF) over heterogeneous landscape. As a case study, the methodology was applied to the Tibetan Plateau area. Eight images of MODIS data (17 January 2003, 14 April 2003, 23 July 2003 and 16 October 2003; 30 January 2007, 15 April 2007, 1 August 2007 and 25 October 2007) and four images of AVHRR data (17 January 2003, 14 April 2003, 23 July 2003 and 16 October 2003) were used in this study for the comparison among winter, spring, summer and autumn and the annual variation analysis. The derived results were also validated by using the "ground truth" measured in the stations of the Tibetan Observation and Research Platform (TORP) and the CAMP/Tibet (CEOP (Coordinated Enhanced Observing Period) Asia-Australia Monsoon Project (CAMP) on the Tibetan Plateau). The results show that the derived EF in four different seasons over the Tibetan Plateau area is in good accordance with the land surface status. The EF show a wide range due to the strong contrast of surface features over the Tibetan Plateau. Also, the estimated EF is in good agreement with the ground measurements, and their absolute percent difference (APD) is less than 10% in the validation sites. The results from AVHRR were also in agreement with MODIS, with the latter usually displaying a higher level of accuracy. It is therefore concluded that the proposed methodology is successful for the retrieval of EF using the MODIS data, AVHRR data and in-situ data over the Tibetan Plateau area, and the MODIS data is the better one and it should be used widely for the evapotranspiration (ET) research over this region.

scholarly journals Oat hay as winter feed improves digestibility, nitrogen balance and energy utilization of Tibetan sheep (Ovis aries) in the Qinghai Tibetan Plateau

2019 ◽  
Vol 230 ◽  
pp. 103854
Author(s):  
Nadir Abdelraheem ◽  
Fuhou Li ◽  
Penghui Guo ◽  
Yi Sun ◽  
Yang Liu ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Nitrogen Balance ◽  
Ovis Aries ◽  
Energy Utilization ◽  
Tibetan Sheep

scholarly journals Serological study of toxoplasmosis spread among unmarried female university students using LAT, ELISA and IgG avidity

2016 ◽  
Vol 13 (4) ◽  
pp. 714-720
Author(s):  
Baghdad Science Journal
Keyword(s):  
Toxoplasma Gondii ◽  
Elisa Test ◽  
Latex Agglutination ◽  
Female Students ◽  
Infectious Agents ◽  
Serum Samples ◽  
Serological Tests ◽  
Worldwide Distribution ◽  
Igm Elisa ◽  
Positive Results

Toxoplasma gondii has a worldwide distribution and it is one of the most prevalent infectious agents in Iraq. The study was conducted on 200 serum samples of unmarried female university of students age ranged between 18 to 26 years to detect Toxoplasma gondii antibodies. The aim of this study was to detect T. gondii antibodies among unmarried female students in Iraqi universities using different serological tests. Seventy six (38%) serum samples out of 200 subjects were positive for toxoplasma antibodies by Latex agglutination test (LAT). Among 76 LAT sera positive ,only 58 (29%) serum samples were positive with toxoplasma IgG ELISA test , however , the results of IgM ELISA assay were positive only for 3 (1.5%) unmarried female sample .None of negative LAT serum samples gave positive results with neither IgG nor IgM ELISA.

scholarly journals SEROPREVALENCE OF INFECTION WITH TOXOPLASMA GONDII AMONG THE RESIDENTS OF SOUTH BACKA DISTRICT, SERBIA

2019 ◽  
Vol 12 (1) ◽  
pp. 33-41
Author(s):  
Ivana Hrnjaković Cvjetković ◽  
Vesna Milošević ◽  
Tamaš Petrović ◽  
Dušan Petrić ◽  
Jelena Radovanov ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Acute Infection ◽  
Automatic Device ◽  
Asymptomatic Infection ◽  
Elisa Test ◽  
Past Infection ◽  
Recent Infection ◽  
Wide Range ◽  
Avidity Test ◽  
Life Threatening

Toxoplasma gondii is a ubiquitous coccidian protozoan that can infect a wide range of warm-blooded animals, including humans. For immunocompetent humans it is a well-adapted parasite that usually causes asymptomatic infection. However, in congenitally infected infants and immunocompromised patients it can cause a serious life threatening disease. The aim of this study was to determine the levels of anti-toxoplasma antibodies among residents of the South Backa District, Serbia. During the period from January 2014 to December 2018, sera from 11,288 persons from South Backa District were tested on the presence of IgM and IgG antibodies against Toxoplasma gondii, using ELISA test (Euroimmun, Lübeck, Germany). The testing was performed on the automatic device Euroimmun Analyzer I-2P. The avidity of IgG WNV antibodies was determined for IgG positive sera using commercial avidity test (Euroimmun, Lübeck, Germany). In total, out of 11,288 patients who were tested for toxoplasma antibodies, the results were positive for 2,513 (22.26%). In 2014 seropositivity for Toxoplasma gondii was 25.78% (464/1800), in 2015 it was 23.30% (400/1717), in 2016 it amounted 20.99% (474/2258), in 2017 it was 21.47% (529/2464) and in 2018 seropositivity was 20.96% (639/3049). Seropositivity of 26.53% (390/1470) was found in males and it amounted 21.62% (2123/9818) in females. Possible recent infection within the last 12 months was found in 1.70% (192/11288) patients. Possible acute infection or false - positive IgM result was detected in 1.31% (148/11288) patients. Past infection was found in 2173/11288 (19.25%) patients. Equivocal results were found in 1.48% of samples (167/11288). The lowest frequency of anti-toxoplasma antibodies was detected in pre-school children 13.07% (97/742) and the highest in persons older than 65 years 60.18% (65/108). Comparing the results of the research done from 2014 to 2018 with the data from 1989, a significant decline of seroprevalence in general population and women of generative age was found.

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