scholarly journals The Distribution of Puumala orthohantavirus Genome Variants Correlates with the Regional Landscapes in the Trans-Kama Area of the Republic of Tatarstan

Pathogens ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1169
Author(s):  
Yuriy N. Davidyuk ◽  
Emmanuel Kabwe ◽  
Anton F. Shamsutdinov ◽  
Anna V. Knyazeva ◽  
Ekaterina V. Martynova ◽  
...  
Keyword(s):  
Bank Vole ◽  
Phylogenetic Analyses ◽  
Genetic Material ◽  
Hemorrhagic Fever ◽  
Federal District ◽  
Genetic Lineage ◽  
Bank Voles ◽  
European Part Of Russia ◽  
The Republic ◽  
River Valleys

In the European part of Russia, the highest number of hemorrhagic fever with renal syndrome (HFRS) cases are registered in the Volga Federal District (VFD), which includes the Republic of Tatarstan (RT). Puumala orthohantavirus (PUUV) is the main causative agent of HFRS identified in the RT. The goal of the current study is to analyze the genetic variations of the PUUV strains and possible presence of chimeric and reassortant variants among the PUUV strains circulating in bank vole populations in the Trans-Kama area of the RT. Complete S segment CDS as well as partial M and L segment coding nucleotide sequences were obtained from 40 PUUV-positive bank voles and used for the analysis. We found that all PUUV strains belonged to RUS genetic lineage and clustered in two subclades corresponding to the Western and Eastern Trans-Kama geographic areas. PUUV strains from Western Trans-Kama were related to the previously identified strain from Teteevo in the Pre-Kama area. It can be suggested that the PUUV strains were introduced to the Teteevo area as a result of the bank voles’ migration from Western Trans-Kama. It also appears that physical obstacles, including rivers, could be overcome by migrating rodents under favorable circumstances. Based on results of the comparative and phylogenetic analyses, we propose that bank vole distribution in the Trans-Kama area occurred upstream along the river valleys, and that watersheds could act as barriers for migrations. As a result, the diverged PUUV strains could be formed in closely located populations. In times of extensive bank vole population growth, happening every 3–4 years, some regions of watersheds may become open for contact between individual rodents from neighboring populations, leading to an exchange of the genetic material between divergent PUUV strains.

scholarly journals Prevalence of the Puumala orthohantavirus Strains in the Pre-Kama Area of the Republic of Tatarstan, Russia

Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 540 ◽  
Author(s):  
Yuriy Davidyuk ◽  
Anton Shamsutdinov ◽  
Emmanuel Kabwe ◽  
Ruzilya Ismagilova ◽  
Ekaterina Martynova ◽  
...  
Keyword(s):  
Hemorrhagic Fever ◽  
Federal District ◽  
European Part ◽  
Genome Segment ◽  
Genetic Lineage ◽  
Geographical Distance ◽  
Mild Form ◽  
Bank Voles ◽  
European Part Of Russia ◽  
The Republic

Puumala orthohantavirus (PUUV) causes nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS) commonly diagnosed in Europe. The majority of HFRS cases in the European part of Russia are diagnosed in the Volga Federal District, which includes the Republic of Tatarstan (RT). The current study aims to analyze the genetic variability of PUUV in Pre-Kama region of the RT bounded by the Volga, Kama, and Vyatka rivers. In 2017, bank voles were caught in seven isolated forest traps in the Pre-Kama region and for the 26 PUUV-positive samples, the partial small (S), medium (M), and large (L) genome segment sequences were obtained and analyzed. It was determined that all identified PUUV strains belong to the Russian (RUS) genetic lineage; however, the genetic distance between strains is not directly correlated with the geographical distance between bank vole populations. One of the identified strains has S and L segments produced from one parental strain, while the M segment was supplied by another, suggesting that this strain could be the reassortant. We suggest that the revealed pattern of the PUUV strains distribution could be the result of a series of successive multidirectional migratory flows of the bank voles to the Pre-Kama region in the postglacial period.

scholarly journals Detection and Genetic Characterization of Puumala Orthohantavirus S-Segment in Areas of France Non-Endemic for Nephropathia Epidemica

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 721
Author(s):  
Séverine Murri ◽  
Sarah Madrières ◽  
Caroline Tatard ◽  
Sylvain Piry ◽  
Laure Benoit ◽  
...  
Keyword(s):  
Amino Acid ◽  
Bank Vole ◽  
Phylogenetic Analyses ◽  
Hemorrhagic Fever ◽  
Nephropathia Epidemica ◽  
Amino Acid Sequences ◽  
Bank Voles ◽  
History Of ◽  
Endemic Areas

Puumala virus (PUUV) in Europe causes nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS). The incidence of NE is highly heterogeneous spatially, whereas the geographic distribution of the wild reservoir of PUUV, the bank vole, is essentially homogeneous. Our understanding of the processes driving this heterogeneity remains incomplete due to gaps in knowledge. Little is known about the current distribution and genetic variation of PUUV in the areas outside the well-identified zones of NE endemicity. We trapped bank voles in four forests in French regions in which NE is considered non-endemic, but sporadic NE cases have been reported recently. We tested bank voles for anti-PUUV IgG and characterized the S segment sequences of PUUV from seropositive animals. Phylogenetic analyses revealed specific amino-acid signatures and genetic differences between PUUV circulating in non-endemic and nearby NE-endemic areas. We also showed, in temporal surveys, that the amino-acid sequences of PUUV had undergone fewer recent changes in areas non-endemic for NE than in endemic areas. The evolutionary history of the current French PUUV clusters was investigated by phylogeographic approaches, and the results were considered in the context of the history of French forests. Our findings highlight the need to monitor the circulation and genetics of PUUV in a larger array of bank vole populations, to improve our understanding of the risk of NE.

scholarly journals Detection of the RNA for new multicomponent virus in patients with Crimean-Congo hemorrhagic fever in southern Russia

2020 ◽  
Author(s):  
Vladimir Ternovoi ◽  
Anastasia Gladysheva ◽  
Alexandra Sementsova ◽  
Anna Zaykovskaya ◽  
Anna Volynkina ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Hemorrhagic Fever ◽  
European Part ◽  
Viral Rna ◽  
Crimean Congo Hemorrhagic Fever ◽  
Viral Genomes ◽  
Cchf Virus ◽  
European Part Of Russia ◽  
Human Sera ◽  
Southern Russia

Background: Recently, a new multicomponent RNA-containing virus was described and called as Jingmen tick virus (JMTV) supposedly belonging to flaviviruses. A virus consists of four viral particles and JMTV was firstly isolated from ticks in China and South America. Aims: Detection viral RNA specific for JMTV complex, sequencing genome fragments and taxonomy identification novel virus from JMTV complex in patients with Crimean-Congo hemorrhagic fever (CCHF) from southern European part of Russia. Materials and methods: Panel of 20 randomly selected sera from patients with confirmed Crimean-Congo hemorrhagic fever was collected in 2016 and was used for detection JMTV and CCHF viral RNA by PCR with experimental primers. Subsequent sequencing of isolated fragments of viral genomes was used for identification JMTV and CCHF virus genetic materials and phylogenetic analyses. Results: The viral RNAs of the CCHF virus and JMTV were detected in blood of four patients. Sequencing of the isolated PCR fragment of S segment CCHF virus allowed identifying these RNA isolates as Europe 1 lineage, subgroups Va and Vb of the CCHF virus that is a typical for the southern European part of the Russia. The nucleotide sequences of segment 2 (GP glycoprotein) of the JMTV were also detected by RP PCR and sequencing in these human sera. The new JMTV isolates were clustered together by phylogenetic analysis. The level of nucleotide identity for newly discovered JMTV isolates was only about 81-82% with comparison to the previously described European variants (Kosovo) of the JMTV. Conclusions: The results suggest that viral genomic RNA for new multicomponent flavivirus named as Manych virus and related to the JMTV complex was discovered in sera of CCHF patients in Russia.

scholarly journals Nephropathia epidemica in Norway: antigen and antibodies in rodent reservoirs and antibodies in selected human populations

1984 ◽  
Vol 93 (1) ◽  
pp. 139-146 ◽  
Author(s):  
T. Traavik ◽  
Ann-Inger Sommer ◽  
R. Mehl ◽  
B. P. Berdal ◽  
K. Stavem ◽  
...  
Keyword(s):  
Bank Vole ◽  
Fluorescent Antibody ◽  
Hemorrhagic Fever ◽  
Nephropathia Epidemica ◽  
Clethrionomys Glareolus ◽  
Distribution Area ◽  
Human Populations ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Bank Voles

SUMMARYNephropathia epidemica (NE) antigen was detected by IFAT (indirect fluorescent antibody technique) in the lungs of 14 of 97 bank voles (Clethrionomys glareolus) collected in three endemic areas. The distribution of antigen positive voles within an endemic location was scattered. Antibodies to Korean hemorrhagic fever (KHF) virus antigens were detected by IFAT in 12 of 14 NE antigen positive bank voles and in 15 of 83 that were antigen negative. NE antigen positive voles exhibited higher antibody titres. Antibodies to KHF were demonstrated in sera from C. rutilus and C. rufocanus collected more than 200 km north of the distribution area for C. glareolus. It appears likely that these vole species can serve as virus vectors for NE cases occurring north of the bank vole area. NE antibodies cross-reacting with KHF virus seem to diminish with time after infection in some NE patients, while for others such cross-reacting antibodies were detected up to 12 years after the disease. Antibodies to KHF were detected in eight of 106 healthy forestry workers with no clinical history of NE. No serological cross-reactions were detected between NE/KHF antigens and representative Bunyaviridae present in Norway. NE/KHF-like viruses appear widespread in Norway, both within and outside of the distribution area of the bank vole.

scholarly journals Accumulation of point mutations and reassortment of genomic RNA segments are involved in the microevolution of Puumala hantavirus in a bank vole (Myodes glareolus) population

2008 ◽  
Vol 89 (7) ◽  
pp. 1649-1660 ◽  
Author(s):  
Maria Razzauti ◽  
Angelina Plyusnina ◽  
Heikki Henttonen ◽  
Alexander Plyusnin
Keyword(s):  
Genetic Diversity ◽  
Bank Vole ◽  
Local Population ◽  
Point Mutations ◽  
Myodes Glareolus ◽  
Stabilizing Selection ◽  
Genetic Lineage ◽  
Nucleotide Substitutions ◽  
Bank Voles ◽  
L Segment

The genetic diversity of Puumala hantavirus (PUUV) was studied in a local population of its natural host, the bank vole (Myodes glareolus). The trapping area (2.5×2.5 km) at Konnevesi, Central Finland, included 14 trapping sites, at least 500 m apart; altogether, 147 voles were captured during May and October 2005. Partial sequences of the S, M and L viral genome segments were recovered from 40 animals. Seven, 12 and 17 variants were detected for the S, M and L sequences, respectively; these represent new wild-type PUUV strains that belong to the Finnish genetic lineage. The genetic diversity of PUUV strains from Konnevesi was 0.2–4.9 % for the S segment, 0.2–4.8 % for the M segment and 0.2–9.7 % for the L segment. Most nucleotide substitutions were synonymous and most deduced amino acid substitutions were conservative, probably due to strong stabilizing selection operating at the protein level. Based on both sequence markers and phylogenetic clustering, the S, M and L sequences could be assigned to two groups, ‘A’ and ‘B’. Notably, not all bank voles carried S, M and L sequences belonging to the same group, i.e. SAMALA or SBMBLB. A substantial proportion (8/40, 20 %) of the newly characterized PUUV strains possessed reassortant genomes such as SBMALA, SAMBLB or SBMALB. These results suggest that at least some of the PUUV reassortants are viable and can survive in the presence of their parental strains.

scholarly journals First isolation and genetic characterization of Puumala orthohantavirus strains from France

2020 ◽  
Author(s):  
Johann Vulin ◽  
Séverine Murri ◽  
Sarah Madrières ◽  
Maxime Galan ◽  
Caroline Tatard ◽  
...  
Keyword(s):  
Bank Vole ◽  
Endemic Area ◽  
High Throughput Sequencing ◽  
Hemorrhagic Fever ◽  
Amino Acid Sequences ◽  
Myodes Glareolus ◽  
Wild Rodents ◽  
Bank Voles ◽  
A Cell ◽  
The Impact

AbstractPuumala orthohantavirus (PUUV) causes a mild form of hemorrhagic fever with renal syndrome (HFRS) named nephropathia epidemica (NE), regularly diagnosed in Europe. France represents the Western frontier of NE expansion in Europe with two distinct areas: the endemic area (Northeast) where PUUV circulates in rodent populations and where many cases of NE are detected in humans and non-endemic area (Southwest) where the virus is not detected and only a few human cases have been reported. The country is a pertinent target to study factors that influence the evolution of PUUV distribution. In this study, we describe for the first time the isolation of two PUUV strains from two distinct French geographical areas: Ardennes (endemic area) and Loiret (non-endemic area). To isolate PUUV efficiently, we selected wild rodents (Myodes glareolus, the specific reservoir of PUUV) from these areas that were seronegative for anti-PUUV IgG (ELISA) but associated with viral RNA load in lung (qRT-PCR). With this design, we are able to cultivate and maintain these two strains in VeroE6 cells but also to propagate efficiently and rapidly both strains in a bank vole colony. Complete coding sequences of S and M segments were determined by Sanger sequencing of RNA extracted from positive bank voles (naturally and experimentally infected) and from supernatant of Vero E6. For the M segment, nucleotidic sequences were 100% identical for both strains. For the S segment, the amino acid sequences from each strain revealed one mismatch between sequences obtained from tissue and from supernatant, revealing a “bank vole” and a “cell” signature. High throughput sequencing confirmed Sanger results, and provided a better assessment of the impact of isolation methods on intra-host viral diversity.

scholarly journals Analysis of Epidemiological Situation on Crimean Hemorrhagic Fever in the Russian Federation in 2020 and Prognosis for 2021

2021 ◽  
pp. 17-22
Author(s):  
A. S. Volynkina ◽  
O. V. Maletskaya ◽  
O. N. Skudareva ◽  
I. V. Tishchenko ◽  
E. I. Vasilenko ◽  
...  
Keyword(s):  
Russian Federation ◽  
Hemorrhagic Fever ◽  
Federal District ◽  
Natural Focus ◽  
Modes Of Transmission ◽  
The North ◽  
The Russian Federation ◽  
The Republic ◽  
Epidemiological Situation

The review presents an analysis of the epidemiological and epizootiological situation on Crimean hemorrhagic fever in the Russian Federation in 2020. During the stated period, 32 CHF cases were registered, which is the minimum indicator since the activation of the natural focus of CHF in 1999. The mortality rate was 3.1 %. The incidence was recorded in the Rostov, Astrakhan Regions, Stavropol Territory, the Republics of Dagestan and Kalmykia. A significant decrease in the incidence of CHF was noted in all entities of the Southern Federal District and the North-Caucasian Federal District. An imported from the Republic of Crimea case of CHF was detected in Moscow. The seasonality of morbidity, occupational, and age composition of CHF patients, modes of transmission, features of the clinical course of the disease in 2020 corresponded to the data of long-term observations. Epizootiological monitoring of the territory of the CHF natural focus showed that the abundance of Hyalomma marginatum adults and the percentage of Ixodidae tick pools positive for the presence of CCHF virus markers corresponded to the average long-term indicators at stationary observation points in 2020 , which indicates the persisting epizootiological disadvantage of the territory of the natural CHF focus in the Russian Federation. The persisting high numbers and infection rate of H. marginatum ticks can contribute to the development of an unfavorable epidemiological situation in the south of the country with a possible increase in the incidence of CHF in the Russian Federation in 2021. 

scholarly journals Microevolution of bank voles (Myodes glareolus) at neutral and immune-related genes during multiannual dynamic cycles: Consequences for Puumala hantavirus epidemiology

2016 ◽  
Author(s):  
Dubois Adelaïde ◽  
Maxime Galan ◽  
Jean-François Cosson ◽  
Bertrand Gauffre ◽  
Heikki Henttonen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Flow ◽  
Bank Vole ◽  
Hemorrhagic Fever ◽  
Research Area ◽  
Density Fluctuations ◽  
Myodes Glareolus ◽  
Bank Voles ◽  
Active Research ◽  
Immune Related Genes

ABSTRACTUnderstanding how host dynamics, including variations of population size and dispersal, may affect the epidemiology of infectious diseases through ecological and evolutionary processes is an active research area. Here we focus on a bank vole (Myodes glareolus) metapopulation surveyed in Finland between 2005 and 2009. Bank vole is the reservoir of Puumala hantavirus (PUUV), the agent of nephropathia epidemica (NE, a mild form of hemorrhagic fever with renal symptom) in humans.M glareoluspopulations experience multiannual density fluctuations that may influence the level of genetic diversity maintained in bank voles, PUUV prevalence and NE occurrence. We examine bank vole metapopulation genetics at presumably neutral markers and immune-related genes involved in susceptibility to PUUV (Tnf-promoter,Mhc-Drb, Tlr4,Tlr7andMx2gene) to investigate the links between population dynamics, microevolutionary processes and PUUV epidemiology. We show that genetic drift slightly and transiently affects neutral and adaptive genetic variability within the metapopulation. Gene flow seems to counterbalance its effects during the multiannual density fluctuations. The low abundance phase may therefore be too short to impact genetic variation in the host, and consequently viral genetic diversity. Environmental heterogeneity does not seem to affect vole gene flow, which might explain the absence of spatial structure previously detected in PUUV in this area. Besides, our results suggest the role of vole dispersal on PUUV circulation through sex-specific and density-dependent movements. We find little evidence of selection acting on immune-related genes within this metapopulation. Footprint of positive selection is detected atTlr-4gene in 2008 only. We observe marginally significant associations betweenMhc-Drbhaplotypes and PUUV serology, and betweenMx2genotype and PUUV genogroups. These results show that microevolutionary changes and PUUV epidemiology in this metapopulation are mainly driven by neutral processes, although the relative effects of neutral and adaptive forces could vary temporally with density fluctuations.

scholarly journals Cytokine, Chemokine, and Metalloprotease Activation in the Serum of Patients with Nephropathia Epidemica from the Republic of Tatarstan and the Republic of Mordovia, Russia

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 527
Author(s):  
Ekaterina Martynova ◽  
Yuriy Davidyuk ◽  
Emmanuel Kabwe ◽  
Ekaterina E. Garanina ◽  
Venera Shakirova ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Hemorrhagic Fever ◽  
Human Infection ◽  
Nephropathia Epidemica ◽  
Matrix Metalloproteases ◽  
Genetic Lineage ◽  
Serum Cytokine ◽  
Serum Samples ◽  
Leukocyte Chemotaxis ◽  
The Republic

Nephropathia Epidemica (NE), endemic to several Volga regions of Russia, including the Republic of Tatarstan (RT) and the Republic of Mordovia (RM), is a mild form of hemorrhagic fever with renal syndrome caused by infection with rodent-borne orthohantaviruses. Although NE cases have been reported for decades, little is known about the hantavirus strains associated with human infection in these regions. There is also limited understanding of the pathogenesis of NE in the RT and the RM. To address these knowledge gaps, we conducted comparative analyses of patients with NE in the RT and the RM. Clinical symptoms were more severe in patients with NE from the RM with longer observed duration of fever symptoms and hospitalization. Analysis of patient sera showed changes in the levels of numerous cytokines, chemokines, and matrix metalloproteases (MMPs) in patients with NE from both the RT and the RM, suggesting leukocyte activation, extracellular matrix degradation, and leukocyte chemotaxis. Interestingly, levels of several cytokines were distinctly different between patients NE from the RT when compared with those from the RM. These differences were not related to the genetic variation of orthohantaviruses circulating in those regions, as sequence analysis showed that Puumala virus (PUUV) was the causative agent of NE in these regions. Additionally, only the “Russia” (RUS) genetic lineage of PUUV was detected in the serum samples of patients with NE from both the RT and the RM. We therefore conclude that differences in serum cytokine, chemokine, and MMP levels between the RT and the RM are related to environmental factors and lifestyle differences that influence individual immune responses to orthohantavirus infection.

scholarly journals How Bank Vole-PUUV Interactions Influence the Eco-Evolutionary Processes Driving Nephropathia Epidemica Epidemiology—An Experimental and Genomic Approach

Pathogens ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 789
Author(s):  
Sarah Madrières ◽  
Caroline Tatard ◽  
Séverine Murri ◽  
Johann Vulin ◽  
Maxime Galan ◽  
...  
Keyword(s):  
Bank Vole ◽  
Hemorrhagic Fever ◽  
Cross Infection ◽  
Nephropathia Epidemica ◽  
Serological Response ◽  
Mild Form ◽  
Bank Voles ◽  
Genomic Approach ◽  
Endemic Strain ◽  
Excretory Organs

In Europe, Puumala virus (PUUV) is responsible for nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS). Despite the presence of its reservoir, the bank vole, on most of French territory, the geographic distribution of NE cases is heterogeneous and NE endemic and non-endemic areas have been reported. In this study we analyzed whether bank vole-PUUV interactions could partly shape these epidemiological differences. We performed crossed-experimental infections using wild bank voles from French endemic (Ardennes) and non-endemic (Loiret) areas and two French PUUV strains isolated from these areas. The serological response and dynamics of PUUV infection were compared between the four cross-infection combinations. Due to logistical constraints, this study was based on a small number of animals. Based on this experimental design, we saw a stronger serological response and presence of PUUV in excretory organs (bladder) in bank voles infected with the PUUV endemic strain. Moreover, the within-host viral diversity in excretory organs seemed to be higher than in other non-excretory organs for the NE endemic cross-infection but not for the NE non-endemic cross-infection. Despite the small number of rodents included, our results showed that genetically different PUUV strains and in a lesser extent their interaction with sympatric bank voles, could affect virus replication and diversity. This could impact PUUV excretion/transmission between rodents and to humans and in turn at least partly shape NE epidemiology in France.

Sign in / Sign up

Export Citation Format

Share Document