scholarly journals Zoonotic Genotypes of Enterocytozoon bieneusi in Wild Living Invasive and Native Carnivores in Poland

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1478
Author(s):  
Agnieszka Perec-Matysiak ◽  
Kinga Leśniańska ◽  
Katarzyna Buńkowska-Gawlik ◽  
Dorota Merta ◽  
Marcin Popiołek ◽  
...  
Keyword(s):  
Native Species ◽  
Its Region ◽  
Enterocytozoon Bieneusi ◽  
Pathogen Transmission ◽  
Gene Marker ◽  
Rrna Gene ◽  
Red Foxes ◽  
Wild Carnivores ◽  
Carnivore Species ◽  
Raccoon Dogs

Wild carnivores, both introduced and native species, are able to adapt well to peri-urban environments, facilitating cross-species pathogen transmission with domestic animals, and potentially humans. The role of wild living reservoir hosts cannot be ignored because of their known carriage of E. bieneusi zoonotic genotypes. In the past decades, populations of wild living carnivores, i.e., native, such as red foxes, and invasive, such as raccoon dogs and raccoons, have increased and adapted to synanthropic environments across Europe, including Poland. The knowledge concerning E. bieneusi genotype identification and distribution in wild carnivores is limited worldwide. A total of 322 individual fecal samples from six carnivore species, i.e., raccoon, raccoon dog, red fox, European badger, pine and beech martens, were collected and then analysed for the presence of E. bieneusi using the nested PCR method. Overall prevalence of the pathogen was estimated to be as high as 27.3%. The infection rates for E. bieneusi varied between the carnivore species, from 13.7% in beech martens to 40.4% in raccoon dogs. Based on sequence analysis of the ITS region of the rRNA gene marker, we detected five known genotypes of E. bieneusi in examined animals. In the invasive species, E. bieneusi NCF2 and D genotypes have been identified, whereas in the native ones, E. bieneusi NCF2, D, C, EbCar2 and Type IV genotypes were identified. All E. bieneusi genotypes recorded in this survey clustered in Group 1, showing their zoonotic potential. Our results provide the first description of the occurrence and genotypes of the microsporidian E. bieneusi in wild living population of raccoon dogs in Europe. Our findings are important for the study of pathogen epidemiology and emphasize the fact that the invasive and the native wild living carnivores, both widely distributed, should be considered more seriously as significant sources of zoonotic pathogens hazardous to domestic and farmed animals and humans.

scholarly journals The role of wild carnivores in the maintenance of trichinellosis in the Tatras National Park, Slovakia

2007 ◽  
Vol 44 (1) ◽  
pp. 18-20 ◽  
Author(s):  
Z. Hurníková ◽  
B. Chovancová ◽  
D. Bartková ◽  
P. Dubinský
Keyword(s):  
National Park ◽  
Brown Bear ◽  
Red Foxes ◽  
Trichinella Britovi ◽  
Wild Carnivores ◽  
Carnivore Species ◽  
Wild Predators

AbstractA survey on trichinellosis in wild carnivores and their role in the transmission of parasite in the model territory of the Tatras National Park was conducted in years 2005–2006. Muscle samples of 102 wild predators representing 10 species belonging to families Canidae, Mustelidae and Ursidae were examined. The highest prevalence of Trichinella britovi was established among red foxes (19.7 %) and martens (37.5 %). The parasite was also detected in European polecat and brown bear. No infection was present in wolves, badgers, otters, weasels, and stoats. The results show that in circulation of trichinellosis in nature various carnivore species are included. The high number of infected martens is indicative of their role in maintenance of trichinellosis that should be considered due to synantropic behaviour of these animals.

scholarly journals Host-adaptation of the rare Enterocytozoon bieneusi genotype CHN4 in Myocastor coypus (Rodentia: Echimyidae) in China

2020 ◽  
Author(s):  
Fuchang Yu ◽  
Yangwenna Cao ◽  
Haiyan Wang ◽  
Qiang Liu ◽  
Aiyun Zhao ◽  
...  
Keyword(s):  
Infection Rate ◽  
Ribosomal Rna ◽  
Pcr Amplification ◽  
Its Region ◽  
Host Adaptation ◽  
Enterocytozoon Bieneusi ◽  
Rrna Gene ◽  
Myocastor Coypus ◽  
Gastrointestinal Pathogen ◽  
Infection Source

Abstract Background: Enterocytozoon bieneusi is a zoonotic gastrointestinal pathogen and can infect both humans and animals. The coypu (Myocastor coypus) is a semi-aquatic rodent, in which few E. bieneusi infections have been reported and the distribution of genotypes and zoonotic potential remains unknown.Methods: A total of 308 fresh fecal samples were collected from seven coypu farms in China to determine the infection rate and the distribution of genotypes of E. bieneusi from coypus using nested-PCR amplification of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene.Results: Enterocytozoon bieneusi was detected with an infection rate of 41.2% (n = 127). Four genotypes were identified, including three known genotypes (CHN4 (n = 111), EbpC (n = 8) and EbpA (n = 7)) and a novel genotype named CNCP1 (n = 1). Conclusions: The rare genotype CHN4 was the most common genotype in the present study, and the transmission dynamics of E. bieneusi in coypus were different from other rodents. To the best of our knowledge, this is the first report of E. bieneusi infections in coypus in China. Our study reveals that E. bieneusi in coypus may be a potential infection source to humans.

scholarly journals Occurrence and potentially zoonotic genotypes of Enterocytozoon bieneusi in wild rhesus macaques (Macaca mulatta) living in Nanwan Monkey Island, Hainan, China: a public health concern

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Wei Zhao ◽  
Huan-Huan Zhou ◽  
Guang-Xu Ren ◽  
Yu Qiang ◽  
Hui-Cong Huang ◽  
...  
Keyword(s):  
Public Health ◽  
Macaca Mulatta ◽  
Rhesus Macaques ◽  
Its Region ◽  
Enterocytozoon Bieneusi ◽  
Health Concern ◽  
Pcr Analysis ◽  
Rrna Gene ◽  
Internal Transcribed Spacer Region ◽  
Type Iv

Abstract Background Enterocytozoon bieneusi, a microsporidian species, is a zoonotic pathogen found in both humans and animals. Here, we determined the prevalence, explored the different genotypes of E. bieneusi in wild rhesus macaques (Macaca mulatta) (Hainan Island of China), and assessed their zoonotic potential. Methods We collected 173 fecal specimens from wild rhesus macaques living in Nanwan Monkey Island, Hainan, China. Subsequently, we identified and genotyped E. bieneusi using nested PCR analysis amplification of the internal transcribed spacer region (ITS) of the rRNA gene. Lastly, a neighbor-joining tree was built based on gene sequences from the ITS region of E. bieneusi. Results Of the 173 specimens from wild rhesus macaques, 26 (15%) were infected with E. bieneusi. We identified six genotypes of E. bieneusi, of which five were known: PigEBITS7 (n = 20), D (n = 2), Type IV (n = 1), Peru6 (n = 1), Henan-III (n = 1), and a novel genotype: HNM-IX (n = 1). From the phylogenetic analysis, the six genotypes identified here were all clustered into zoonotic group 1. Conclusion This study is the first report to detect E. bieneusi infection in wild rhesus macaques from Hainan, China. Human-pathogenic genotypes D, Henan-III, Peru6, PigEbITS7, and Type IV in the wild rhesus macaques support these animals infected with E. bieneusi have a public health significance.

scholarly journals “Enterocytozoon bieneusi and Cryptosporidium: a cross-sectional study conducted throughout Thailand”

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Rapeepun Prasertbun ◽  
Hirotake Mori ◽  
Yaowalark Sukthana ◽  
Supaluk Popruk ◽  
Teera Kusolsuk ◽  
...  
Keyword(s):  
Rural Areas ◽  
Its Region ◽  
Epidemiological Data ◽  
Cross Sectional Study ◽  
Small Subunit ◽  
Enterocytozoon Bieneusi ◽  
Rrna Gene ◽  
Intestinal Protozoa ◽  
Cross Sectional ◽  
Epidemiological Characteristics

Abstract Background Enterocytozoon bieneusi and Cryptosporidium spp. are prevalent zoonotic parasites associated with a high burden among children. To date only limited molecular epidemiological data on E. bieneusi and Cryptosporidium spp. in humans living in Thailand has been published. Methods PCR-based tools were used to detect and characterize E. bieneusi and Cryptosporidium spp. The internal transcribed spacer (ITS) region of the rRNA gene was used to investigate E. bieneusi, and the small subunit (SSU) rRNA gene was used to investigate Cryptosporidium spp., and 697 fecal samples from villagers and school children in rural areas in Thailand were analyzed. Results The infection rates were 2.15% (15/697) for E. bieneusi and 0.14% (1/697) for Cryptosporidium spp. The prevalence of E. bieneusi was significantly high in Loei province. Sequence analysis indicated that the Cryptosporidium isolate was C. parvum. Nine E. bieneusi genotypes were identified, EbpC, Peru12, TMH6, TMH3, TMH7, H, D, and two novel genotypes TMLH1 and TMLH2. E. bieneusi prevalence was significantly higher in male participants than in female participants, and in children aged 3–15 years than in participants aged > 15 years. Conclusions The prevalence, genotypes, and zoonotic potential of E. bieneusi were found to vary significantly high even in one country. Transmission routes and key animal carriers of E. bieneusi may be associated with differences in hygiene, sanitation, and cultural behaviors. Further molecular studies including longitudinal studies will be required to unveil epidemiological characteristics of these opportunistic intestinal protozoa in all over the countries.

scholarly journals Occurrence and genetic diversity of piroplasms and other apicomplexa in wild carnivores

2016 ◽  
Vol 2 ◽  
Author(s):  
J. F. BARANDIKA ◽  
A. ESPÍ ◽  
B. OPORTO ◽  
A. DEL CERRO ◽  
M. BARRAL ◽  
...  
Keyword(s):  
Abundant Species ◽  
Meles Meles ◽  
Domestic Animals ◽  
18S Rrna Gene ◽  
Ixodid Ticks ◽  
Rrna Gene ◽  
Northern Spain ◽  
Red Foxes ◽  
Wild Carnivores ◽  
Pcr Targeting

SUMMARYTick-borne apicomplexan haemoparasites infect wild and domestic animals, but studies on their distribution among free-living animals are comparatively fewer. Tissues from 241 wild carnivores of eight Mustelidae, two Canidae, one Viverridae and one Felidae species were collected in Northern Spain, and analysed by real-time PCR targeting the 18S rRNA gene and sequencing.Babesia vulpes(formerly known asTheileria annae) was the only piroplasm detected in red foxes (Vulpes vulpes). Badgers (Meles meles) were shown to harbour two novelBabesiasp. sequence types (A and B) that only shared 96.7% homology between them and were closely related (ca. 97–98%) to, but distinct fromB. vulpesand other babesia from carnivores. Analysis of PCR-derived sequences also revealed the presence ofCystoisosporasp. andHepatozoonsp. in badgers and showed that wild cats (Felis silvestris catus) were infected withCytauxzoonsp. Forty-two per cent of the animals subjected to a detailed external examination were parasitized by ixodid ticks, beingIxodes hexagonusandIxodes ricinusthe most abundant species. This study provided novel data on the different haemoparasites that can infect European wild carnivores and showed that they can be hosts for a range of haemoparasites and pose a potential risk for transmission to domestic animals.

scholarly journals Host-adaptation of the rare Enterocytozoon bieneusi genotype CHN4 in Myocastor coypus (Rodentia: Echimyidae) in China

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Fuchang Yu ◽  
Yangwenna Cao ◽  
Haiyan Wang ◽  
Qiang Liu ◽  
Aiyun Zhao ◽  
...  
Keyword(s):  
Infection Rate ◽  
Ribosomal Rna ◽  
Pcr Amplification ◽  
Its Region ◽  
Host Adaptation ◽  
Enterocytozoon Bieneusi ◽  
Rrna Gene ◽  
Myocastor Coypus ◽  
Gastrointestinal Pathogen ◽  
Infection Source

Abstract Background Enterocytozoon bieneusi is a zoonotic gastrointestinal pathogen and can infect both humans and animals. The coypu (Myocastor coypus) is a semi-aquatic rodent, in which few E. bieneusi infections have been reported and the distribution of genotypes and zoonotic potential remains unknown. Methods A total of 308 fresh fecal samples were collected from seven coypu farms in China to determine the infection rate and the distribution of genotypes of E. bieneusi from coypus using nested-PCR amplification of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene. Results Enterocytozoon bieneusi was detected with an infection rate of 41.2% (n = 127). Four genotypes were identified, including three known genotypes (CHN4 (n = 111), EbpC (n = 8) and EbpA (n = 7)) and a novel genotype named CNCP1 (n = 1). Conclusions The rare genotype CHN4 was the most common genotype in the present study, and the transmission dynamics of E. bieneusi in coypus were different from other rodents. To the best of our knowledge, this is the first report of E. bieneusi infections in coypus in China. Our study reveals that E. bieneusi in coypus may be a potential infection source to humans.

scholarly journals Host-adaptation of Rare Enterocytozoon bieneusi genotype CHN4 in Coypus (Myocastor coypus) in China

2020 ◽  
Author(s):  
Fuchang Yu ◽  
Yangwenna Cao ◽  
Haiyan Wang ◽  
Qiang Liu ◽  
Aiyun Zhao ◽  
...  
Keyword(s):  
Infection Rate ◽  
Ribosomal Rna ◽  
Pcr Amplification ◽  
Its Region ◽  
Host Adaptation ◽  
Enterocytozoon Bieneusi ◽  
Rrna Gene ◽  
Myocastor Coypus ◽  
Gastrointestinal Pathogen ◽  
Infection Source

Abstract Background: Enterocytozoon bieneusi is a zoonotic gastrointestinal pathogen and can infect both humans and animals. Coypus (Myocastor coypus) are semi-aquatic rodents, in which few E. bieneusi infections have been reported and the distribution of genotypes and zoonotic potential remains unknown.Methods: A total of 308 fresh fecal samples were collected from seven coypu farms in China to determine the infection rate and the distribution of genotypes of E. bieneusi from coypus using nested-PCR amplification of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene.Results: E. bieneusi was detected with an infection rate of 41.2% (n = 127). Four genotypes were identified, including three known genotypes: CHN4 (n = 111), EbpC (n = 8) and EbpA (n = 7) and a novel genotype named CNCP1 (n = 1). Conclusions: The rare genotype CHN4 was the most common one in the present study, and the transmission dynamics of E. bieneusi in coypus were different from other rodents. This is the first report of E. bieneusi infections in coypus in China. Our study reveals that E. bieneusi in coypus may be potential infection source to humans.

scholarly journals Abrupt Temporal Fluctuations in the Chicken Fecal Microbiota Are Explained by Its Gastrointestinal Origin

2012 ◽  
Vol 78 (8) ◽  
pp. 2941-2948 ◽  
Author(s):  
M. Sekelja ◽  
I. Rud ◽  
S. H. Knutsen ◽  
V. Denstadli ◽  
B. Westereng ◽  
...  
Keyword(s):  
Barrier Function ◽  
16S Rrna Gene Sequencing ◽  
Fecal Microbiota ◽  
Pathogen Transmission ◽  
Rrna Gene ◽  
Multivariate Statistical ◽  
Content Type ◽  
Factors Affecting ◽  
Temporal Fluctuations ◽  
Gastric Barrier

ABSTRACTOne of the main challenges in understanding the composition of fecal microbiota is that it can consist of microbial mixtures originating from different gastrointestinal (GI) segments. Here, we addressed this challenge for broiler chicken feces using a direct 16S rRNA gene-sequencing approach combined with multivariate statistical analyses. Broiler feces were chosen because of easy sampling and the importance for pathogen transmission to the human food chain. Feces were sampled daily for 16 days from chickens with and without a feed structure-induced stimulation of the gastric barrier function. Overall, we found four dominant microbial phylogroups in the feces. Two of the phylogroups were related to clostridia, one to lactobacilli, and one toEscherichia/Shigella. The relative composition of these phylogroups showed apparent stochastic temporal fluctuations in feces. Analyses of dissected chickens at the end of the experiment, however, showed that the two clostridial phylogroups were correlated to the microbiota in the cecum/colon and the small intestine, while the upper gut (crop and gizzard) microbiota was correlated to the lactobacillus phylogroup. In addition, chickens with a stimulated gizzard also showed less of the proximate GI dominating bacterial group in the feces, supporting the importance of the gastric barrier function. In conclusion, our results suggest that GI origin is a main determinant for the chicken fecal microbiota composition. This knowledge will be important for future understanding of factors affecting shedding of both harmful and beneficial gastrointestinal bacteria through feces.

scholarly journals Savitreea pentosicarens gen. nov., sp. nov., a yeast species in the family Saccharomycetaceae isolated from a grease trap

2020 ◽  
Vol 70 (11) ◽  
pp. 5665-5670
Author(s):  
Varunya Sakpuntoon ◽  
Jirameth Angchuan ◽  
Chanita Boonmak ◽  
Pannida Khunnamwong ◽  
Noémie Jacques ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Yeast Species ◽  
Sequence Divergence ◽  
Its Region ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
D2 Domain ◽  
The Family ◽  
Lsu Rrna Gene ◽  
Lsu Rrna

Two strains (DMKU-GTCP10-8 and CLIB 1740) representing a novel anamorphic yeast species were isolated from a grease sample collected from a grease trap in Thailand and from an unidentified fungus collected in French Guiana, respectively. On the basis of phylogenetic analysis based on the combined D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, Lachancea fermentati CBS 707T was the closely related species with 12.8 % sequence divergence (70 nucleotide substitutions and three gaps in 571 nucleotides) and 28.1 % sequence divergence (93 nucleotide substitutions and 90 gaps in 651 nucleotides) in the D1/D2 domain of the LSU rRNA gene and the ITS region, respectively. Phylogenetic analysis based on the concatenated sequences of the five genes including the small subunit rRNA gene, the D1/D2 domain of the LSU rRNA gene, the ITS region, translation elongation factor-1 alpha (TEF1) and RNA polymerase II subunit 2 (RPB2) genes confirmed that the two strains (DMKU-GTCP10-8 and CLIB 1740) were well-separated from other described yeast genera in Saccharomycetaceae. Hence, Savitreea pentosicarens gen. nov., sp. nov. is proposed to accommodate these two strains as members of the family Saccharomycetaceae. The holotype is S. pentosicarens DMKU-GTCP10-8T (ex-type strain TBRC 12159=PYCC 8490; MycoBank number 835044).

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