scholarly journals Biomaterials for Soft Tissue Repair and Regeneration: A Focus on Italian Research in the Field

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1341
Author(s):  
Maria Cristina Bonferoni ◽  
Carla Caramella ◽  
Laura Catenacci ◽  
Bice Conti ◽  
Rossella Dorati ◽  
...  
Keyword(s):  
Tissue Repair ◽  
Biological Response ◽  
Tissue Response ◽  
Biomedical Science ◽  
Bioactive Materials ◽  
Tissue Repair And Regeneration ◽  
Interdisciplinary Field ◽  
Advanced Therapy ◽  
Living Tissues

Tissue repair and regeneration is an interdisciplinary field focusing on developing bioactive substitutes aimed at restoring pristine functions of damaged, diseased tissues. Biomaterials, intended as those materials compatible with living tissues after in vivo administration, play a pivotal role in this area and they have been successfully studied and developed for several years. Namely, the researches focus on improving bio-inert biomaterials that well integrate in living tissues with no or minimal tissue response, or bioactive materials that influence biological response, stimulating new tissue re-growth. This review aims to gather and introduce, in the context of Italian scientific community, cutting-edge advancements in biomaterial science applied to tissue repair and regeneration. After introducing tissue repair and regeneration, the review focuses on biodegradable and biocompatible biomaterials such as collagen, polysaccharides, silk proteins, polyesters and their derivatives, characterized by the most promising outputs in biomedical science. Attention is pointed out also to those biomaterials exerting peculiar activities, e.g., antibacterial. The regulatory frame applied to pre-clinical and early clinical studies is also outlined by distinguishing between Advanced Therapy Medicinal Products and Medical Devices.

scholarly journals Adaptive immunity and skin wound healing in amphibian adults

2019 ◽  
Vol 14 (1) ◽  
pp. 420-426 ◽  
Author(s):  
Antonella Franchini
Keyword(s):  
Wound Healing ◽  
Tissue Repair ◽  
Tissue Injury ◽  
Skin Wound ◽  
Tissue Response ◽  
Cellular Mechanisms ◽  
Skin Wound Healing ◽  
Whole Process ◽  
Tissue Repair And Regeneration ◽  
Adaptive Immune Cells

AbstractRegeneration and repair with scarring of the skin are two different responses to tissue injury that proceed depending on the animal species. Several studies in multiple organisms have shown that the effectiveness of tissue repair gradually decreases with age in most vertebrates, while the molecular and cellular mechanisms underlying the diverse potentials remain incompletely understood. It is clear, however, that immune system actively participates in the whole process and immune-related activities can mediate both negative and positive roles to influence the quality and diversity of tissue response to damage. Compared with innate immunity, our understanding of the significance of adaptive immune cells in normal repair outcome is limited and deserves further investigation. Here, experimental evidence supporting the contribution of lymphocytes and the involvement of lymphoid organs in skin wound healing are discussed, focusing on the findings emerged in adult amphibians, key animal models for tissue repair and regeneration research.

Biomimetic Bone Graft with Higher Bioactivity

2007 ◽  
Vol 330-332 ◽  
pp. 943-946 ◽  
Author(s):  
Roberta Martinetti ◽  
L. Dolcini ◽  
Luisia Merello ◽  
Silvia Scaglione ◽  
Rodolfo Quarto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Tissue Repair ◽  
Experimental Approach ◽  
Physiological Activity ◽  
Physical Support ◽  
Support Matrix ◽  
Living Tissues

The use of 3D osteoconductive scaffolds provides an informative substrate serving as a physical support matrix for in vivo tissue regeneration. In the last few years the use of bioengineered 3D scaffolds has been becoming the most promising experimental approach for the regeneration of living tissues. Stem cells are typically used, in combination with 3D substrates, to promote in vivo bone regeneration and repair. For tissue engineering applications, biomaterials should therefore be able to support the functional properties of osteo-progenitor cells, giving them the optimal microenvironment to perform their physiological activity. Inorganic biomaterials are particularly relevant for bone regeneration; calcium phosphate ceramics have in fact been shown to strongly interact with bone tissue. The aim of the present work was to evaluate two different scaffolds with a defined design and different composition developed to guide/promote tissue repair.

The role of polyethylene wear in joint replacement failure

1997 ◽  
Vol 211 (1) ◽  
pp. 65-72 ◽  
Author(s):  
M A McGee ◽  
D W Howie ◽  
S D Neale ◽  
D R Haynes ◽  
M J Pearcy
Keyword(s):  
Aseptic Loosening ◽  
Joint Replacement ◽  
Polyethylene Wear ◽  
Biological Response ◽  
Tissue Response ◽  
Cause Of Failure ◽  
Polyethylene Particles

Aseptic loosening is the major cause of failure of joint replacement prostheses. Polyethylene implants removed at revision surgery regularly show wear. It is proposed that the polyethylene particles released into the tissues as a consequence of this wear induce a tissue response that precedes aseptic loosening. This paper presents the results of recent in vivo and in vitro studies of the biological response to polyethylene wear particles undertaken in the authors' laboratories. A clinical perspective is provided by the inclusion of the authors' recent observations of retrieval analyses of joint replacement implant wear and the tissue response to polyethylene in humans.

In vitro and in vivo evaluation of the effect of nano-sized collagen molecules and nicotinamide on mesenchymal stem cell differentiation

2016 ◽  
Vol 4 (22) ◽  
pp. 3892-3902 ◽  
Author(s):  
Chin-Tsu Ma ◽  
Yi-Jhen Wu ◽  
Han Hsiang Huang ◽  
Pei-Leun Kang ◽  
Kuan Yin Hsiao ◽  
...  
Keyword(s):  
Tissue Repair ◽  
Stem Cell Differentiation ◽  
In Vivo Evaluation ◽  
Cell Replacement ◽  
Tissue Repair And Regeneration ◽  
Mesenchymal Stem Cell Differentiation ◽  
Collagen Molecules ◽  
Stromal Stem Cells

Advances and improvements in mesenchymal stromal/stem cells (MSCs) and cell replacement therapies have been promising approaches to treat diabetes mellitus (DM) since their potent capacities for differentiation into various functional cells match the demands of tissue repair and regeneration.

scholarly journals Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Hengwei Jin ◽  
Kuo Liu ◽  
Juan Tang ◽  
Xiuzhen Huang ◽  
Haixiao Wang ◽  
...  
Keyword(s):  
Tissue Repair ◽  
Reliable Method ◽  
Additional Data ◽  
Pleural Cavity ◽  
Genetic System ◽  
Lineage Tracing ◽  
Tissue Repair And Regeneration ◽  
Genetic Targeting ◽  
Organ Invasion

AbstractDuring injury, monocytes are recruited from the circulation to inflamed tissues and differentiate locally into mature macrophages, with prior reports showing that cavity macrophages of the peritoneum and pericardium invade deeply into the respective organs to promote repair. Here we report a dual recombinase-mediated genetic system designed to trace cavity macrophages in vivo by intersectional detection of two characteristic markers. Lineage tracing with this method shows accumulation of cavity macrophages during lung and liver injury on the surface of visceral organs without penetration into the parenchyma. Additional data suggest that these peritoneal or pleural cavity macrophages do not contribute to tissue repair and regeneration. Our in vivo genetic targeting approach thus provides a reliable method to identify and characterize cavity macrophages during their development and in tissue repair and regeneration, and distinguishes these cells from other lineages.

Bioglass Middle Ear Prosthesis: Preliminary Report

1986 ◽  
Vol 95 (1) ◽  
pp. 78-82 ◽  
Author(s):  
Gerald E. Merwin
Keyword(s):  
Middle Ear ◽  
Tissue Response ◽  
Current Status ◽  
Ear Reconstruction ◽  
Bioactive Materials ◽  
Hearing Results ◽  
Middle Ear Prosthesis ◽  
Early Results ◽  
Living Tissues ◽  
Middle Ear Reconstruction

The current status of materials for use in middle ear reconstruction is discussed with particular emphasis given to alloplastic implants including bioinert and bioactive materials. Bioglass is a bioactive transparent glass which forms a bond with living tissues. Its development and characteristics are discussed, and the early results of a clinical trial using a Bioglass middle ear prosthesis are presented. The satisfactory tissue response and hearing results found in this preliminary group of patients encourage further clinical evaluation of the Bioglass middle ear prosthesis for use in ossicular reconstruction.

scholarly journals Assessment of the Bone Healing Process Mediated by Periosteum-Derived Mesenchymal Stem Cells’ Secretome and a Xenogenic Bioceramic—An In Vivo Study in the Rabbit Critical Size Calvarial Defect Model

Materials ◽  
2021 ◽  
Vol 14 (13) ◽  
pp. 3512
Author(s):  
Mindaugas Pranskunas ◽  
Egidijus Šimoliūnas ◽  
Milda Alksne ◽  
Victor Martin ◽  
Pedro Sousa Gomes ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Critical Size ◽  
Healing Process ◽  
Rabbit Model ◽  
Biological Response ◽  
Calvarial Bone ◽  
Tissue Repair And Regeneration ◽  
Tissue Healing ◽  
Bone Tissue Formation

The mesenchymal stem cell (MSC) secretome has been considered an innovative therapeutic biological approach, able to modulate cellular crosstalk and functionality for enhanced tissue repair and regeneration. This study aims to evaluate the functionality of the secretome isolated from periosteum-derived MSCs, from either basal or osteogenic-induced conditions, in the healing of a critical size calvarial bone defect in the rabbit model. A bioceramic xenograft was used as the vehicle for secretome delivery, and the biological response to the established biocomposite system was assessed by clinical, histological, histomorphometric, and microtomographic analysis. A comparative analysis revealed that the osteogenic-induced secretome presented an increased diversity of proteins, with emphasis on those related to osteogenesis. Microtomographic and histological morphometric analysis revealed that bioceramic xenografts implanted with secretomes enhanced the new bone formation process, with the osteogenic-induced secretome inducing the highest bone tissue formation. The application of the MSC secretome, particularly from osteogenic-induced populations, may be regarded as an effective therapeutic approach to enhance bone tissue healing and regeneration.

scholarly journals The Application of Three-Dimensional Collagen-Scaffolds Seeded with Myoblasts to Repair Skeletal Muscle Defects

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Jianqun Ma ◽  
Kyle Holden ◽  
Jinhong Zhu ◽  
Haiying Pan ◽  
Yong Li
Keyword(s):  
Soft Tissue ◽  
Muscle Tissue ◽  
Tissue Repair ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Tissue Repair And Regeneration ◽  
Tissue Constructs ◽  
Defect Sites

Three-dimensional (3D) engineered tissue constructs are a novel and promising approach to tissue repair and regeneration. 3D tissue constructs have the ability to restore form and function to damaged soft tissue unlike previous methods, such as plastic surgery, which are able to restore only form, leaving the function of the soft tissue often compromised. In this study, we seeded murine myoblasts (C2C12) into a collagen composite scaffold and cultured the scaffold in a roller bottle cell culture system in order to create a 3D tissue graftin vitro. The 3D graft createdin vitrowas then utilized to investigate muscle tissue repairin vivo. The 3D muscle grafts were implanted into defect sites created in the skeletal muscles in mice. We detected that the scaffolds degraded slowly over time, and muscle healing was improved which was shown by an increased quantity of innervated and vascularized regenerated muscle fibers. Our results suggest that the collagen composite scaffold seeded with myoblasts can create a 3D muscle graftin vitrothat can be employed for defect muscle tissue repairin vivo.

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