scholarly journals Green Synthesized Silver Nanoparticles Using Tridax Procumbens for Topical Application: Excision Wound Model and Histopathological Studies

Pharmaceutics ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1754
Author(s):  
Farhat Fatima ◽  
Mohammed F. Aldawsari ◽  
Mohammed Muqtader Ahmed ◽  
Md. Khalid Anwer ◽  
Maimuna Naz ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Particle Analysis ◽  
Histopathological Study ◽  
Treated Animal ◽  
Excision Wound ◽  
Wound Model ◽  
Tridax Procumbens ◽  
Bet Analysis ◽  
Green Synthesized Silver Nanoparticles

The objective of this study was to synthesize silver nanoparticles from the leaves of Tridax procumbens and develop its topical gels using chitosan to investigate the wound healing efficacy concomitant with the histopathological study. Green synthesized silver nanoparticles (AgNPs) were prepared by reacting silver nitrate (0.3 M) with leaf extract and characterized by particle analysis, FTIR, XRD, SEM, BET, and TGA. The results revealed formed AgNPs were nano-sized (138 ± 2.1 nm), monodispersed (PDI: 0.460 ± 0.3), inter-particle repulsion (zeta: −20.4 ± 5.20 mV), stabilized, crystalline and, spherical with size ranging from 80–100 nm as per SEM micro photos. The BET analysis of AgNPs presents the surface area (12.861 m2/g), pore volume (0.037 cc/g), and pore radius (24.50 nm).TGA results show a loss of 13.39% up to 300 °C. The topical formulation was developed by loading AgNPs in chitosan-based gels, evaluated by pH, thermal cycling, centrifugal, and spreadability tests. AgNPs chitosan gels results showed skin compatibility, higher stability, and spreading ability. The maximum antibacterial zone of inhibition was found to be 25 ± 0.98 mm for bacillus subtitles and 30 ± 1.99 mm for Klebsiella pneumoniae, respectively. Nanosilver-containing gel also showed excellent compatibility with erythrocytes. Excision wound model was used to assess the wound healing property of the developed AgNP gels, the results of which indicated a significantly progressive healing process in test-group of animals treated with chitosan-based gels containing AgNPs. A histopathological study further confirmed the almost normal skin structure of treated animal tissue compared to standard and negative control. Thus, green synthesized AgNPs loaded chitosan-based topical gel can potentially be used for wound healing application.

scholarly journals EFFECT OF HEALOL OIL ON WOUND HEALING IN WISTAR RATS: A NOVEL STUDY

2018 ◽  
Vol 11 (3) ◽  
pp. 152
Author(s):  
Sujith V ◽  
Poornima G ◽  
Balaji O ◽  
Bairy Kl ◽  
Praveen K ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Chronic Wounds ◽  
Statistical Significance ◽  
Silver Sulfadiazine ◽  
Histopathological Analysis ◽  
Burn Wound ◽  
Excision Wound ◽  
Burn Wounds ◽  
Wound Model

 Objective: Wound healing is a complex process, and various plant extracts have been used to study the effect of medicinal plants on wound healing. Healol oil is used in some tribal areas for ulcer healing and treatment of burn wounds. There are no animal studies done so far using healol oil to find out its role in the treatment of wounds. Hence, the aim of our study is to find the effect of healol oil in excision as well burn wounds in Wistar rats.Methods: Thirty-six Wistar rats were used, 18 rats in excision wound model and remaining in burn wound model. Each model consists of three groups of six rats each. Povidone-iodine was used as a standard control in excision wound model and silver sulfadiazine was used as a standard control in burn wound model. Healol oil was used as test drug in both the models. Period of epithelization and wound contracture rates were analyzed. Histopathological analysis of the skin tissue was done. One-way analysis of variance was used followed by Tukey’s post hoc test for statistical analysis using SPSS version 23 software, P value of <0.05 was taken as statistical significance.Results: Both standard and test groups showed significantly faster wound healing (p<0.001) compared to control rats treated with paraffin wax in both the models. In excision model standard povidone was significantly (p<0.001) better compared to healol oil whereas in burn model, silver sulfadiazine and healol oil showed comparable results with respect to period of epithelization without statistical significance (p>0.05).Conclusion: Wound healing property of healol oil is proved, and further clinical trial can be done to find out the effect of healol oil on chronic wounds due to various etiologies.

scholarly journals EVALUATION OF WOUND HEALING ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII (A. DC.) IN WISTAR ALBINO RATS

2014 ◽  
Vol 1 (1) ◽  
pp. 73-77
Author(s):  
Uma Maheswari P ◽  
Shalimol A ◽  
Arumugasamy A ◽  
Udhaya Sankar M.R
Keyword(s):  
Wound Healing ◽  
Dry Weight ◽  
Wound Contraction ◽  
Albino Rats ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Model ◽  
Wistar Albino Rats ◽  
Incision Wound ◽  
Wound Healing Activity

The present experimental study was designed to evaluate the wound healing activity of methanolic extract of Smilax wightii A. DC. on incision and excision wound models in Wistar albino rats. The parameters studied were wound breaking strength, wound contraction area, epithelialization period, granulation tissuewet, dry weight and hydroxyproline content in incision wound model, percentage of wound contraction and period of epithelialization in excision wound model. The rats were administered topically with 100mg/kg b.wt. (low dosage), 200mg/kg b.wt. (moderate dosage) and 500mg/kg b.wt. (high dosage) of methanolicextract of Smilax wightii (MESW). The activity of the extract treated groups were compared with that of the control 1% Spirit. Framycetin sulphate 0.2% w/w was used as the standard drug. In incision wound model, there was a significant increase in the wound breaking strength in all the experimental groups treated withMESW than that of the control. Similarly, significant (P<0.001) decrease in wound contraction area and period of epithelialization were also observed in the test group animals treated with MESW and the standard drug treated groups when compared to that of the control. , a significant increase was observed in granulation tissue wet and dry weight and hydroxyproline content in the test groups treated with MESW compared to the control. In exicision wound model, there was a significant increase (P<0.01) in the percentage of wound contraction and decrease in period of epithelialization in the experimental groups treated with 200mg/kgb.wt. (moderate dosage) and 500mg/kgb.wt (high dosage) of MESW. The extract treated groups showed significant improvement in all the wound healing parameters of incision, and excision wound models.

Wound healing potential of green synthesized silver nanoparticles prepared from Lansium domesticum fruit peel extract

2015 ◽  
Vol 5 (2) ◽  
pp. 159-164 ◽  
Author(s):  
Shiv Shankar ◽  
Lily Jaiswal ◽  
Rangabhatla Sai Laxmi Aparna ◽  
Rangabhatla GunneswaraS V Prasad ◽  
Govindappa Prem Kumar ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Fruit Peel ◽  
Green Synthesized Silver Nanoparticles ◽  
Lansium Domesticum ◽  
Peel Extract

scholarly journals Determination of wound healing activity of Shark liver oil using the excision wound model in Wistar rats

2020 ◽  
Vol 11 (4) ◽  
pp. 7301-7308
Author(s):  
Auti S A ◽  
Jat R K ◽  
Das S K ◽  
Shukla S T
Keyword(s):  
Wound Healing ◽  
Disease Control ◽  
Wistar Rats ◽  
Chronic Wounds ◽  
Wound Contraction ◽  
Standard Drug ◽  
Wound Area ◽  
Excision Wound ◽  
Shark Liver Oil ◽  
Wound Model

There are various promising preclinical models, such as in mice, wistar rats, rabbits and pigs, which can be utilized to initiate acute or chronic wounds. These can be persuaded by many distinctive techniques, with excision the most common. After determining a proper model for a study, investigators need to choose an appropriate and reproducible technique that will permit the monitoring of the wound improvement over time. In this study, the healing power of Shark Liver Oil Emulgel (SLO) in Wistar rats were analyzed by using the excision wound model. The shark liver oil was prepared as emulgel at a concentration of 5%,10% and 15%, respectively and both Standard drug(Povidone-Iodine Ointment USP 5% w/w-PI) and SLO is applied at a concentration of 1mg/mm2; Topically. The parameters integrated for the assessment of the effects of SLO were Relative body weight changes, wound area contraction in mm2, relative wound percentage and epithelialization time. Wound area contraction was measured on 1st, 3rd, 6th, 9th, 12th, 15th, 18th and 21st day. Wistar rats treated with SLO showed substantial variations in epithelialization period and improved wound contraction in the excision wound treatment as compared to disease control. The biochemical biomarkers like SOD(Superoxide dismutases), GSH(Reduced Glutathione)and LPO(Lipid peroxidase) in the treated group have shown a significant change in the improvement of wound healing. Histopathological studies and microscopic observations specify that the topical use of Shark liver oil Emulgel extensively improved wound contraction, collagenation and epithelialization with well-organized dermis devoid of inflammatory cells in contrast to disease control.

WOUND HEALING ACTIVITY OF TRIPHALA IN EXPERIMENTALLY INDUCED DIABETIC RATS

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (04) ◽  
pp. 70-75
Author(s):  
M. P. Singh ◽  
S Vashisht ◽  
V. Chawla ◽  
P. Kumari ◽  
Keyword(s):  
Wound Healing ◽  
Oral Administration ◽  
Dead Space ◽  
Diabetic Rats ◽  
Wound Contraction ◽  
Control Group ◽  
Excision Wound ◽  
Wound Model ◽  
Diabetic Wound Healing ◽  
Healing Property

Aim of the present study was to explore the wound healing potential of Triphala extract. Triphala ointment (15% w/w) was used in the study for excision wound model. For dead space wound model Triphala extract (200 mg/kg) was administered orally. Extract of Triphala topically applied in excision wound model was found to possess wound healing property. On day 11th, the extract treated animals showed wound contraction to the extent of 87.63% and 94.77%, compared with control group of animals. In dead space wound model, oral administration of the extract was found to increase the wet and dry granuloma weight of tissue. The extract treated animals showed high quantity of hydroxyproline content when compared with the control group animals. The topical and oral administration of Triphala extract promotes diabetic wound healing in streptozotocin induced diabetic rats with increased rate of wound contraction and collagen turnover.

WOUND HEALING ACTIVITY OF THE LEAVES OF WRIGHTIA TINCTORIA (ROXB) R. BR

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (03) ◽  
pp. 40-46
Author(s):  
M. C. Divakar ◽  
◽  
S Lakshmi Devi
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Excision Wound ◽  
Wound Model ◽  
Methanolic Extracts ◽  
Wrightia Tinctoria ◽  
Incision Wound ◽  
Wound Healing Activity

The study was aimed to evaluate wound healing activity of 70% ethanolic and methanolic extracts of leaves of Wrightia tinctoria (Roxb) R. Br (Apocynaceae) using incision and excision wound models on Wistar rats. Wound contraction and period of epithelization were assessed in excision wound model whereas wound tensile strength was determined in case of incision wound model. 4% Wrightia tinctoria methanolic leaf extract phytosome exhibited significant wound healing potential when compared with standard 0.2% nitrofurazone ointment.

WOUND HEALING ACTIVITY OF ETHANOLIC EXTRACT OF FICUS RACEMOSA LEAVES

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (11) ◽  
pp. 15-24
Author(s):  
V.P Londhe ◽  
◽  
S. S Nipate ◽  
A.H. Tiwari ◽  
P.D. Chaudhari
Keyword(s):  
Wound Healing ◽  
Ethanolic Extract ◽  
Biochemical Parameter ◽  
Histopathological Study ◽  
Phytochemical Study ◽  
Wound Model ◽  
Ficus Racemosa ◽  
Incision Wound ◽  
Regeneration Of Skin ◽  
Wound Healing Activity

Ficus racemosa (Family: Moracae) is a prominent plant in India and it was embedded in traditional medicinal texts of India as a wound healer. Hence to study its wound healing assets, the ethanolic leaves extract was studied on animals. Excision and incision wound models were used to study wound healing activity. For both models, extract was given in the form of ointment (5% and 10 % w/w). In the excision model, all extract treated animals showed significant (P<0.01) increase in percentage wound contraction. The incision wound model showed significant (P<0.01) increase in breaking strength when compared to control. In biochemical parameter, hydroxyproline level was significantly (P<0.01) increased in all drugs treated groups as compared to control in the excision wound model. Histopathological study also showed good regeneration of skin compared to control. Phytochemical study of the leaves showed presence of flovonoids and tannins which might be the key constituents for the wound healing activity.

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