scholarly journals Co-Inoculation of Mesorhizobium ciceri with Either Bacillus sp. or Enterobacter aerogenes on Chickpea Improves Growth and Productivity in Phosphate-Deficient Soils in Dry Areas of a Mediterranean Region

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 571
Author(s):  
Imane Benjelloun ◽  
Imane Thami Alami ◽  
Mohamed El Khadir ◽  
Allal Douira ◽  
Sripada M. Udupa
Keyword(s):  
Field Experiments ◽  
Enterobacter Aerogenes ◽  
Dry Weight ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Sp ◽  
Mesorhizobium Ciceri ◽  
Shoot Dry Weight ◽  
Combined Application ◽  
Dry Areas ◽  
Phosphate Solubilizing

Biological nitrogen fixation requires a large amount of phosphorus (P). However, most of the soils are P-deficient and the extensive use of P- chemical fertilizers constitute a serious threat to the environment. In this context, two field experiments were carried out to investigate the effect of co-inoculation of Mesorhizobium ciceri with phosphate solubilizing bacteria (PSB), Bacillus sp., and Enterobacter aerogenes, on chickpea as an alternative to chemical nitrogen (N) and phosphorous fertilizers in P-deficient soils in dry areas of Morocco. The results revealed that combined inoculation of chickpea with rhizobia and PSB showed a significant enhancement of chickpea nodulation, biomass production, yields and N, P, and protein content in grains as compared to single inoculation or single application of N or P. A significantly higher increase was obtained by inoculating chickpea with Mesorhizobium sp. MA72 combined with E. aerogenes P1S6. This combination allowed an enhancement of more than 270% in nodulation, 192% in shoot dry weight and 242% in grain yield. The effect of this combination was equivalent to the effect of combined application of N and P fertilizers. Formulation of biofertilizers based on tasted strains could be used for chickpea co-inoculation in P-deficient soils for an eco-friendly sustainable production of chickpea.

scholarly journals Isolasi Rhizobakteri dan Pengaruh Aplikasinya dengan Pupuk N-P terhadap Mutu Benih dan Pertumbuhan Bibit Tetua Betina Jagung

2018 ◽  
Vol 45 (3) ◽  
pp. 255
Author(s):  
Pitri Ratna Asih ◽  
Memen Surahman ◽  
Dan Giyanto
Keyword(s):  
Field Experiments ◽  
Seed Quality ◽  
Female Parent ◽  
Dry Weight ◽  
Hybrid Seed ◽  
Phosphate Solubilizing Bacteria ◽  
Isolation And Identification ◽  
Seed Field ◽  
P Fertilizer ◽  
Phosphate Solubilizing

Increased productivity of maize can be done with the use of high quality seeds from improved varieties such as hybrid seed. The objectives of this study were Increasing productivity of maize female parent is important in order to reduce the price of hybrid seed. The objectives of this study were to determine the nitrogen fixing bacteria compatible with phosphate solubilizing bacteria (PSB), and using those bacteria to increase physiological seed quality and seedling growth of maize female parent. The research consisted of laboratory and field experiments. Laboratory experiment for the isolation and identification of rhizobacteria resulted in 25 Azotobacter and 29 Actinomycetes non-pathogenic isolates capable of fixing nitrogen and PSB selected for compatibility tests were AB3, B28, P12, P14, P24, and P31. The compatibility test showed 25 pairs of BPF with Azotobacter and 16 pairs of BPF with Actinomycetes were mutually compatible. The BPF pair with Azotobacter or Actinomycetes P24-AzL7, P24-AzL9, B28-AcCKB4, P24-AcCKB9, P24-AcCKB20, and P24-AcCKW5 were able to increase the vigor index of hybrid maize female parent seed. Field experiment was arranged in a split plot design with three replications. The main plot was dosage of N-P fertilizer (0%, 25%, 50%, 75%, and 100% of recommendation dosage), and the subplot was 12 rhizobacteria treatments selected from 25 compatible pairs of BPF with Azotobacter and 16 pairs of BPF with Actinomycetes and 1 control. The application of compatible pairs of bacteria had a significant effect on plant height, the number of leaves at 3 and 4 weeks after planting and plant dry weight. However, the best treatment i.e. B28-AcCKB4 was not significantly different with the nutrient broth treatment (as control).<br /><br /><br />

scholarly journals Sustainable Management with Mycorrhizae and Phosphate Solubilizing Bacteria for Enhanced Phosphorus Uptake in Calcareous Soils

Agriculture ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 334 ◽  
Author(s):  
Fazli Wahid ◽  
Shah Fahad ◽  
Subhan Danish ◽  
Muhammad Adnan ◽  
Zhen Yue ◽  
...  
Keyword(s):  
Sustainable Management ◽  
Field Experiments ◽  
Block Design ◽  
Wheat Yield ◽  
Phosphorus Uptake ◽  
Calcareous Soils ◽  
P Uptake ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Sp ◽  
Phosphate Solubilizing

Low availability of phosphorus (P) in calcareous soils is a major problem for sustainable improvement in cereals crops yield. A higher amount of calcium in soils precipitates the P, thus making it immobile in soil. Inoculation of arbuscular mycorrhizal fungi (AMF) and phosphate solubilizing bacteria (PSB) could be helpful in the sustainable management of immobile P in soil. However, their combined use in releasing P from rock phosphate (RP) in alkaline calcareous soils have been little investigated. In this regard, two successive field experiments were conducted to assess the interactive inoculation potential of AMF and PSB strain Bacillus sp. PIS7 with RP on the yield and P uptake of maize (Zea mays L.) and wheat (Triticum aestivum L.) crops in alkaline calcareous soil. The first field experiment was conducted in a complete randomized block design with 10 treatments and three replications by inoculating maize seeds with AMF and Bacillus sp. PIS7 inoculum alone and in combination with RP. Their performance was compared with single super phosphate (SSP) inorganic fertilizer. Afterward, the residual effects of inoculated AMF and Bacillus sp. PIS7 were investigated on wheat as a subsequent crop. Maize and wheat yield parameters, P uptake, AMF root colonization, and PSB population was measured. The results of both trials indicated the beneficial effects of AMF and Bacillus sp. PIS7 with RP in increasing the plants grain yield and P uptake until the second season after inoculation, as compared to controls. Likewise, maize and wheat roots colonization, PSB population density, and post-harvest soil properties were also improved by the combined inoculation of AMF and Bacillus sp. PIS7 with RP. It is concluded that PSB solubilizes the unavailable forms of P in combination with RP fertilizers in soil, and AMF ultimately transfers it to plants for growth promotion. Moreover, the combined inoculation of AMF and PSB with ground RP had more potential to improve maize-wheat yields and P uptake comparable to those obtained by using expensive phosphatic fertilizers in P deficient calcareous pH soils.

KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Suliasih Suliasih
Keyword(s):  
Alkaline Phosphatase ◽  
Soil Sample ◽  
Rhizosphere Soil ◽  
Botanical Garden ◽  
Soil Enzyme ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Sp ◽  
Phosphate Solubilizing ◽  
Serratia Rubidaea

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 – 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of “Lavender”.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase

Investigation of a chlorsulfuron-resistant chickweed [Stellaria media (L.) Vill.] population

1994 ◽  
Vol 74 (4) ◽  
pp. 693-697 ◽  
Author(s):  
J. T. O'Donovan ◽  
G. M. Jeffers ◽  
M. P. Sharma ◽  
D. Maurice
Keyword(s):  
Field Experiments ◽  
Dry Weight ◽  
Sulfonylurea Herbicides ◽  
Percentage Germination ◽  
Flower Number ◽  
Stellaria Media ◽  
Shoot Dry Weight ◽  
Excellent Control ◽  
Two Populations ◽  
Growth Analyses

A chickweed population (R) from a farm near Stony Plain, Alberta, was more resistant to chlorsulfuron than a population (S) collected near Vegreville, Alberta. In greenhouse experiments, the S population was controlled completely by chlorsulfuron applied at 5 g ha−1, whereas 22 g ha−1 was required to reduce dry weight of the R population by 50%. Experiments conducted in a germinator indicated that percentage germination of the R population was higher than that of the S population up to ~ 60 h. Growth analyses in the greenhouse indicated that leaf number, leaf area, shoot dry weight, days to flowering, flower number, seed weight and relative yields differed little between the two populations. In field experiments, control of the R population was poor with the sulfonylurea herbicides, chlorsulfuron, metsulfuron methyl, triasulfuron, amidosulfuron and thifensulfuron. Good to excellent control was obtained with cyanazine/MCPA, linuron, metribuzin, mecoprop, bentazon, metribuzin + MCPA, linuron + MCPA, and mecoprop + bentazon. Key words: Sulfonylurea herbicides, chlorsulfuron, herbicide resistance, relative competitiveness

scholarly journals Isolation and Characterization of Phosphate-Solubilizing Bacteria from Mushroom Residues and their Effect on Tomato Plant Growth Promotion

2017 ◽  
Vol 66 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Jian Zhang ◽  
Peng Cheng Wang ◽  
Ling Fang ◽  
Qi-An Zhang ◽  
Cong Sheng Yan ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Wood Flour ◽  
Dry Weight ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Sequence Comparisons ◽  
Isolation And Characterization ◽  
Phosphate Solubilizing

Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Highly efficient phosphate-solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. In this study, 13 isolates were obtained from waste mushroom residues, which were composed of cotton seed hulls, corn cob, biogas residues, and wood flour. NBRIP solid medium was used for isolation according to the dissolved phosphorus halo. Eight isolates produced indole acetic acid (61.5%), and six isolates produced siderophores (46.2%). Three highest phosphate-dissolving bacterial isolates, namely, M01, M04, and M11, were evaluated for their beneficial effects on the early growth of tomato plants (Solanum lycopersicum L. Wanza 15). Strains M01, M04, and M11 significantly increased the shoot dry weight by 30.5%, 32.6%, and 26.2%, and root dry weight by 27.1%, 33.1%, and 25.6%, respectively. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, strains M01 and M04 belonged to the genus Acinetobacter, and strain M11 belonged to the genus Ochrobactrum. The findings suggest that waste mushroom residues are a potential resource of plant growth-promoting bacteria exhibiting satisfactory phosphate-solubilizing for sustainable agriculture.

scholarly journals Isolation of Phosphate Solubilizing Bacteria and their Use for Plant Growth Promotion in Tomato Seedling and Plant

2018 ◽  
Vol 13 (2) ◽  
pp. 61-70
Author(s):  
Rajiv Pathak ◽  
Vipassana Paudel ◽  
Anupama Shrestha ◽  
Janardan Lamichhane ◽  
Dhurva. P. Gauchan
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
In Vitro Study ◽  
Dry Weight ◽  
Greenhouse Experiment ◽  
Phosphate Solubilizing Bacteria ◽  
Tomato Seedling ◽  
Phosphate Solubilizing ◽  
Phosphorous Compounds

Phosphorous (P) is an essential macronutrient and most soils contain high levels of P. However, its availability to plant is limited by rapid immobilization of phosphorous compounds to insoluble forms and hence plant available forms of P in soils are found in low amounts. Phosphate solubilizing bacteria provide an eco-friendly alternative to convert insoluble phosphates into plant available forms. In the present study, three phosphate solubilizing bacterial isolates (PB-1, PB-4 and VC-01) with visually significant phosphate solubilizing abilities were isolated from tomato rhizosphere soil. In-vitro study in pikovskaya’s agar revealed that isolate PB-1 had the highest phosphate solubilizing ability with a phosphate solubilizing index of 2.08±0.07 followed by isolate VC-01 (1.31±0.09) and PB-4 (1.24±0.08). Isolates were used as bacterial inoculum to assess their ability to promote tomato (Lycopersicon esculentum var. Srijana) seedling and plant growth in in-vitro and greenhouse experiment respectively. Isolate PB-4 showed best growth promotion in seedling assay whereas isolate PB-1 and VC-01 also promoted seedling growth compared to control. In greenhouse experiment however, isolates VC-01 and PB-1 significantly enhanced all parameters (shoot length, root length, shoot and root dry weight) compared to uninoculated control whereas isolate PB-4 had a positive effect on all parameters except root length.Kathmandu University Journal of Science, Engineering and TechnologyVol. 13, No. 2, 2017, page: 61-70

Management of emerged weeds in irrigated blackgram (Vigna mungo L.) through post-emergence herbicides

2015 ◽  
Author(s):  
T. Ramesh ◽  
S. Rathika
Keyword(s):  
Field Experiments ◽  
Dry Weight ◽  
Cost Ratio ◽  
Treatment Schedule ◽  
Benefit Cost Ratio ◽  
Net Returns ◽  
Combined Application ◽  
Hand Weeding ◽  
Benefit Cost ◽  
Quizalofop Ethyl

Field experiments were conducted during summer (April to June), 2010 and 2011 to study the effect of post-emergence herbicides in irrigated blackgram (ADT 5). Treatments comprising of three levels (50, 75 and 100 g /ha) of the post- emergence herbicides viz., Imazethapyr 10% SL and Quizalofop ethyl 5% EC were tested alone and in combination and compared with hand weeding twice on 15 and 30 DAS(farmers’ practice). Herbicides were sprayed on 15 DAS when the weeds were 2-4 leaves stage using flat-fan nozzle as per treatment schedule. The results revealed that minimum total weed density (8.44) and weed dry weight ( 27.2 g/m2) and higher weed control efficiency (84.4%) were obtained under combined application of imazethapyr and quizalofop ethyl as tank mix at 100 g /ha. However, this was comparable with application of both the herbicides at 75g /ha. Application of imazethapyr + quizalofop ethyl each at 75 g/ha registered significantly taller plants, higher dry matter production, more number of pods per plant, seeds per pod and higher grain yield (826 kg/ha), net returns (Rs.26621/ha) and benefit cost ratio (2.76). Thus, application of post- emergence herbicides imazethapyr + quizalofop ethyl each at 75 g /ha as tank mix on 15 DAS could be recommended for controlling of emerged weeds in irrigated blackgram.

Pre-maturity gradients in shoot size and in number and size of florets for spring barley treated with mepiquat chloride

1988 ◽  
Vol 110 (3) ◽  
pp. 633-639
Author(s):  
S. R. Waddington ◽  
Phyllis Cartwright
Keyword(s):  
Field Experiments ◽  
Spring Barley ◽  
Dry Weight ◽  
Stem Length ◽  
Small Scale ◽  
Main Shoot ◽  
Mepiquat Chloride ◽  
Shoot Dry Weight ◽  
Spike Development ◽  
Shoot Size

SummaryEffects of mepiquat chloride on gradients of shoot growth within spring barley plants (cv. Koru) were determined in two small-scale field experiments. Mepiquat chloride was applied at either the lemma primordium stage or the late carpel primordium stage of spike development in the main shoot. The shoot dry weight, stem length, spike length, number and size of florets and floret developmental score were measured for all shoots, over the period from the lemma primordium stage of main shoot spike development to the late milk stage of grain development.Lemma primordium mepiquat chloride increased the size and developmental score of later-formed shorter shoots pre-anthesis while delaying the spike development of the main shoot and other longer shoots by up to 4 days. The overall effect was to produce plants with a reduced range of shoot and spike sizes from the end of floret initiation in the main shoot until maturity. In addition, there was a slight increase in the number of shoots per plant in both experiments. Gradients of carpel development and carpel width along the spike were reduced in all treated spikes by the time of floret abortion in the main shoot but the effects were more noticeable in shorter shoots. The number of florets initiated per plant was increased by 21–22%. All shoots contributed to the larger number of florets but again the major additions came from the shorter shoots. The few extra florets initiated in longer shoots were lost during abortion of distal florets while the 2–5 extra florets on shorter shoots were retained to give extra grains at maturity.Application of mepiquat chloride at the late carpel primordium stage had little effect on gradients of spike size or on number, size and development of florets.

scholarly journals Selection and evaluation of phosphate-solubilizing bacteria from grapevine rhizospheres for use as biofertilizers

2016 ◽  
Vol 14 (4) ◽  
pp. e1106 ◽  
Author(s):  
Min Liu ◽  
Xu Liu ◽  
Bao-Sen Cheng ◽  
Xue-Lei Ma ◽  
Xiao-Tong Lyu ◽  
...  
Keyword(s):  
Enterobacter Aerogenes ◽  
Acc Deaminase ◽  
Northwest China ◽  
Fertilizer Application ◽  
Vitis Vinifera L ◽  
Cabernet Sauvignon ◽  
Phosphate Solubilizing Bacteria ◽  
Alkaline Soils ◽  
Pgp Traits ◽  
Phosphate Solubilizing

Phosphate-solubilizing bacteria (PSB) have the ability to solubilize insoluble phosphorus (P) and release soluble P. Extensive research has been performed with respect to PSB isolation from the rhizospheres of various plants, but little is known about the prevalence of PSB in the grapevine rhizosphere. In this study, we aimed to isolate and identify PSB from the grapevine rhizosphere in five vineyards of Northwest China, to characterize their plant-growth-promoting (PGP) traits, evaluate the effect of stress on their phosphate-solubilizing activity (PSA), and test their ability to stimulate the growth of Vitis vinifera L. cv. Cabernet Sauvignon. From the vineyard soils, 66 PSB isolates were screened, and 10 strains with high PSA were identified by 16S rRNA sequencing. Sequence analysis revealed that these 10 strains belonged to 4 genera and 5 species: Bacillus aryabhattai, B. megaterium, Klebsiella variicola, Stenotrophomonas rhizophila, and Enterobacter aerogenes. The selected PSB strains JY17 (B. aryabhattai) and JY22 (B. aryabhattai) were positive for multiple PGP traits, including nitrogen fixation and production of indole acetic acid (IAA), siderophores, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, chitinase, and protease. JY17 and JY22 showed strong PSA under stress conditions of high pH, high salt, and high temperature. Therefore, these two isolates can be used as biofertilizers in saline-alkaline soils. The inoculation with PSB significantly facilitated the growth of V. vinifera cv. Cabernet Sauvignon under greenhouse conditions. Use of these PSB as biofertilizers will increase the available P content in soils, minimize P-fertilizer application, reduce environmental pollution, and promote sustainable agriculture.

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