Optimization of a Cryopreservation Method for the Endangered Korean Species Pogostemon yatabeanus Using a Systematic Approach: The Key Role of Ammonium and Growth Regulators

Cryopreservation provides a secure long-term conservation option for rare and endangered plant species with non-orthodox or limitedly available seeds. Wide application of cryopreservation to biobank wild flora is hampered by the need to re-optimize nearly all protocol steps for every new species. We applied a systematic approach to simplify optimization of a multi-stage droplet-vitrification method for the endangered wetland Korean species, Pogostemon yatabeanus. This approach consisted of a standard procedure pre-selected based on material type and size, which was complemented with 11 additional treatments to reveal the most impactful conditions. Effect of ammonium nitrate at various protocol steps was also tested. The highest shoot tip survival (92%) and plant regeneration (90%) after cryopreservation were achieved using preculture with 10% sucrose followed by 40 min osmoprotection and 60 min treatment with vitrification solution A3-80% (33.3% glycerol + 13.3% dimethyl sulfoxide + 13.3% ethylene glycol + 20.1% sucrose) on ice. A three-step regrowth procedure starting with ammonium-free medium with 1 mg/L GA3 and 1 mg/L BA followed by ammonium-containing medium with and without growth regulators was essential for the development of healthy plants from cryopreserved shoot tips. This approach enables fast optimization of the cryopreservation procedure for new osmotic stress-sensitive plant species.

Download Full-text

Suitability of Cryopreservation for the Long-term Storage of Rare and Endangered Plant Species: a Case History for Cosmos atrosanguineus

Annals of Botany ◽

10.1093/aob/mcg009 ◽

2002 ◽

Vol 91 (1) ◽

pp. 65-74 ◽

Cited By ~ 37

Author(s):

T. WILKINSON

Keyword(s):

Plant Species ◽

Case History ◽

Endangered Plant ◽

Endangered Plant Species ◽

Long Term Storage ◽

Rare And Endangered ◽

Term Storage

Download Full-text

Ex situ conservation of plant species of the Red Book of Russia in botanical gardens

АгроЭкоИнфо ◽

10.51419/20212211 ◽

2021 ◽

Vol 2 (44) ◽

pp. 11-11

Author(s):

Alexander Saakian ◽

◽

Keyword(s):

Plant Species ◽

Russian Federation ◽

Ex Situ Conservation ◽

Ex Situ ◽

Endangered Plants ◽

Botanical Gardens ◽

Rare And Endangered ◽

The Russian Federation

Russian botanical gardens are actively involved in the conservation of rare and endangered plants. They pay special attention to the species included in the Red Book of the Russian Federation. At present, 377 species of higher plants are cultivated in living collections of botanical gardens out of 514 species presented in the Red Book of Russia, which is 73%. Thus, the Russian Federation has practically met the requirement of goal 8 of the Global Strategy for Plant Conservation. The vast majority of rare plant species are represented by samples in the collections of many botanical gardens and the reliability of their protection under cultural conditions is beyond doubt. In the Main Botanical Garden of the Russian Academy of Sciences, an in vitro collection of rare and endangered plants is preserved, including 82 species, which is 17.3% of the total number of angiosperms included in the Red Book of the Russian Federation. As a result of many years of research, the features of the cultivation and preservation of ex situ plants belonging to different families have been revealed. The main methodological aspects at the stages of obtaining a sterile culture, micropropagation proper and long-term deposition are reflected. The compositions of nutrient media and cultivation factors were optimized for slowed growth of explants of the studied cultures and preservation of their viability. Keywords: RARE AND ENDANGERED PLANT SPECIES, RED BOOK OF THE RUSSIAN FEDERATION, EX SITU CONSERVATION, LONG-TERM IN VITRO CONSERVATION

Download Full-text

Materials on the distribution of plant species which are rare for the Volgograd oblast

BIO Web of Conferences ◽

10.1051/bioconf/20191600032 ◽

2019 ◽

Vol 16 ◽

pp. 00032

Author(s):

Stepan Senator ◽

Vladimir Vasjukov ◽

Eugene Zibzeev ◽

Andrey Korolyuk

Keyword(s):

Endangered Species ◽

Plant Species ◽

Vascular Plants ◽

Regional Security ◽

Volgograd Oblast ◽

Rare And Endangered Species ◽

Management Measures ◽

Threatened Plant Species ◽

Rare And Endangered

The information about the distribution of 28 rare and endangered species of vascular plants under Federal and regional security, registered in 2018 on the territory of the Volgograd oblast under the Federal and regional security is given. Among them, 2 species (Crambe tataria Sebeok и Stipa zalesskii Wilensky ex P. A. Smirn.) are included in the List of Threatened plant species of Europe. Each location is linked to the grid of Atlas Flora Europaea. The obtained materials about the distribution of rare and endangered species of vascular plants represent an important data for long-term monitoring and also for environmental management measures at the regional level.

Download Full-text

Establishing relationships with a foreign cooperative partner in the context of the implementation of the international cooperation method

Economics. Finances. Law ◽

10.37634/efp.2021.1.5 ◽

2021 ◽

pp. 25-28

Author(s):

Liubov ZALISKA ◽

Liubov HANAS

Keyword(s):

International Cooperation ◽

Graphical Representation ◽

Multi Stage ◽

Cooperative Partner ◽

Common Goals ◽

Search And Selection ◽

Definition Of ◽

Selection Of

Introduction. One of the most important stages of the company's participation in international cooperation is undoubtedly the search and selection of a cooperating partner and establishing relationships with him. This plays a key role in achieving the goals and desired results of companies' participation in international cooperation and requires detailed study, systematization and graphical representation. The purpose of the paper is to improve the participation of enterprises in international cooperation by developing a sequence of establishing relationships with foreign cooperating partners in the context of using a certain method of international cooperation. Results. As participation in international industrial cooperation involves long-term partnership and pooling of resources to achieve common goals, the effectiveness of international cooperation depends directly on the cooperating partner. The process of finding and selecting a cooperating partner, establishing relationships with him is long-term and multi-stage, so it requires a clear definition of its components, their sequence and consistency. Thus, one of the necessary conditions for an enterprise to participate effectively in international cooperation is to develop a clear sequence of establishing relationships with a foreign cooperating partner, which consists of seven stages. The result of the successful completion of each of the stages of the proposed sequence of networking is the signing of an agreement with a foreign cooperating partner on the use of a certain method of international cooperation. If at a certain stage the actual results don`t coincide with the planned ones, it is possible to return to the problem areas and make certain adjustments in order to eliminate the shortcomings. Conclusion. Against the background of globalization and integration processes, the role of international cooperation as one of the effective ways to improve the competitiveness of both individual enterprises and the country's economy as a whole is constantly growing. However, the effectiveness of participation in international cooperation depends not only on the company itself, but also on the chosen cooperating partner. The proposed sequence of establishing relationships with a foreign cooperating partner in the context of the implementation of the method of international cooperation is a practically significant and universal tool that companies can adapt to their own goals and peculiarities and repeatedly apply in the future to choose a cooperating partner.

Download Full-text

Body shaming among school-going adolescents: prevalence and predictors

International Journal of Community Medicine and Public Health ◽

10.18203/2394-6040.ijcmph20201075 ◽

2020 ◽

Vol 7 (4) ◽

pp. 1324

Author(s):

Rahul Taye Gam ◽

Shivendra Kumar Singh ◽

Manish Manar ◽

Sujita Kumar Kar ◽

Abhishek Gupta

Keyword(s):

Cross Sectional Study ◽

Sample Population ◽

Single Gender ◽

Cross Sectional ◽

Multi Stage ◽

One Year ◽

The Individual ◽

Relationship Of

Background: The transition from childhood to adulthood is marked by dramatic physical, mental, sexual, psychological and social developmental changes. These tumultuous years can have long term consequences for the individual, especially when it comes to mental health. Body shaming, weight shaming or appearance-based harassment can be described as the act of mocking or humiliating someone based on their physical appearance. It often leads to low self-esteem, low body dissatisfaction, and depressive symptoms, which is consistent with the growing body of work emphasizing the harmful role of appearance-based harassment among youth. The objectives of the study were to estimate the prevalence of body shaming among school-going adolescents and to assess the distribution and relationship of body shaming with various factors.Methods: A cross-sectional study was conducted in 4 schools of Lucknow with 200 participants from each school. They filled anonymous self-administered questionnaires and as a part of the study their height and weight measurements were also taken. Multi-stage random sampling was done to select the sample population from classes 9th to 12th.Results: A total of 44.9 percent (n=359) participants responded to have been body shamed at least once in the past one year. It is maximum among boys in co-ed schools and least among girls in single-gender schools.Conclusions: The prevalence observed in the present study is higher than global reports, thus drawing attention to the need for similar studies on the subject.

Download Full-text

Role of micro-reserves in conservation of endemic, rare and endangered plants of the Valencian region (Eastern Spain)

Israel Journal of Plant Sciences ◽

10.1080/07929978.2016.1256131 ◽

2016 ◽

Vol 63 (4) ◽

pp. 320-332 ◽

Cited By ~ 8

Author(s):

Emilio Laguna ◽

Simón Fos ◽

Juan Jiménez ◽

Sergei Volis

Keyword(s):

Protected Areas ◽

Plant Species ◽

Endangered Plants ◽

Natural Parks ◽

Internal Network ◽

Rare And Endangered ◽

Valencian Region ◽

Large Protected Areas ◽

Number Of Plant Species

Since 1998 the Valencian Community (Spain) has pioneered the establishment of plant micro-reserves (PMR), which has resulted in a network currently comprising 299 sites. The PMR are compatible with large protected areas including natural parks (NP, 22 areas). In fact, 73 PMR are included within the NP network (internal subnet of PMR) and 226 PMR are outside NP (external subnet). Here we analyze how the PMR network complements that of NP in capturing rare (RS, twp categories), endemic (ES, three categories) and endangered (TS, four categories) plants. The external subnet increases the number of plant species with territorial protection by 10.8% in RS, 15.8% in ES and 21.0% in TS categories. Additionally, when comparing number of species in the external and internal PMR subnets not shared with the alternative subnet type, the former has higher absolute and relative values for the nine categories analyzed. We propose that the internal network should be increased only to capture populations of the species that are not included in the external subnet.

Download Full-text

Coming Together Through Falling Apart

Rorschachiana Journal of the International Society for the Rorschach ◽

10.1027/1192-5604/a000115 ◽

2019 ◽

Vol 40 (2) ◽

pp. 151-168

Author(s):

Heather Churchill ◽

Jeremy M. Ridenour

Keyword(s):

Data Analysis ◽

Qualitative Data ◽

Structural Changes ◽

Systematic Approach ◽

Qualitative Approach ◽

Psychological Assessments ◽

Transference And Countertransference ◽

Testing Data ◽

Psychoanalytically Oriented Psychotherapy

Abstract. Assessing change during long-term psychotherapy can be a challenging and uncertain task. Psychological assessments can be a valuable tool and can offer a perspective from outside the therapy dyad, independent of the powerful and distorting influences of transference and countertransference. Subtle structural changes that may not yet have manifested behaviorally can also be assessed. However, it can be difficult to find a balance between a rigorous, systematic approach to data, while also allowing for the richness of the patient’s internal world to emerge. In this article, the authors discuss a primarily qualitative approach to the data and demonstrate the ways in which this kind of approach can deepen the understanding of the more subtle or complex changes a particular patient is undergoing while in treatment, as well as provide more detail about the nature of an individual’s internal world. The authors also outline several developmental frameworks that focus on the ways a patient constructs their reality and can guide the interpretation of qualitative data. The authors then analyze testing data from a patient in long-term psychoanalytically oriented psychotherapy in order to demonstrate an approach to data analysis and to show an example of how change can unfold over long-term treatments.

Download Full-text