scholarly journals Antineoplastic Activity of Rhus trilobata Nutt. (Anacardiaceae) against Ovarian Cancer and Identification of Active Metabolites in This Pathology

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2074
Author(s):  
Luis Varela-Rodríguez ◽  
Blanca Sánchez-Ramírez ◽  
Erika Saenz-Pardo-Reyes ◽  
José Juan Ordaz-Ortiz ◽  
Rodrigo Daniel Castellanos-Mijangos ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gallic Acid ◽  
Metabolite Profile ◽  
Antineoplastic Activity ◽  
Apoptosis Induction ◽  
Cytostatic Effect ◽  
Active Metabolites ◽  
Active Compounds ◽  
Lipid Compounds ◽  
Mtt Assays

Rhus trilobata (RHTR) is a medicinal plant with cytotoxic activity in different cancer cell lines. However, the active compounds in this plant against ovarian cancer are unknown. In this study, we aimed to evaluate the antineoplastic activity of RHTR and identify its active metabolites against ovarian cancer. The aqueous extract (AE) and an active fraction (AF02) purified on C18-cartridges/ethyl acetate decreased the viability of SKOV-3 cells at 50 and 38 μg/mL, respectively, compared with CHO-K1 (>50 μg/mL) in MTT assays and generated changes in the cell morphology with apoptosis induction in Hemacolor® and TUNEL assays (p ≤ 0.05, ANOVA). The metabolite profile of AF02 showed a higher abundance of flavonoid and lipid compounds compared with AE by UPLC-MSE. Gallic acid and myricetin were the most active compounds in RHTR against SKOV-3 cells at 50 and 166 μg/mL, respectively (p ≤ 0.05, ANOVA). Antineoplastic studies in Nu/Nu female mice with subcutaneous SKOV-3 cells xenotransplant revealed that 200 mg/kg/i.p. of AE and AF02 inhibited ovarian tumor lesions from 37.6% to 49% after 28 days (p ≤ 0.05, ANOVA). In conclusion, RHTR has antineoplastic activity against ovarian cancer through a cytostatic effect related to gallic acid and myricetin. Therefore, RHTR could be a complementary treatment for this pathology.

scholarly journals Consumption of Phenolic-Rich Food and Dietary Supplements as a Key Tool in SARS-CoV-19 Infection

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2084
Author(s):  
José David Flores-Félix ◽  
Ana C. Gonçalves ◽  
Gilberto Alves ◽  
Luís R. Silva
Keyword(s):  
Immune Response ◽  
Angiotensin Converting Enzyme ◽  
Dietary Supplements ◽  
Gallic Acid ◽  
Health Systems ◽  
Converting Enzyme ◽  
Active Compounds ◽  
Angiotensin Converting Enzyme 2 ◽  
High Severity ◽  
Viral Proteases

The first cases of COVID-19, which is caused by the SARS-CoV-2, were reported in December 2019. The vertiginous worldwide expansion of SARS-CoV-2 caused the collapse of health systems in several countries due to the high severity of the COVID-19. In addition to the vaccines, the search for active compounds capable of preventing and/or fighting the infection has been the main direction of research. Since the beginning of this pandemic, some evidence has highlighted the importance of a phenolic-rich diet as a strategy to reduce the progression of this disease, including the severity of the symptoms. Some of these compounds (e.g., curcumin, gallic acid or quercetin) already showed capacity to limit the infection of viruses by inhibiting entry into the cell through its binding to protein Spike, regulating the expression of angiotensin-converting enzyme 2, disrupting the replication in cells by inhibition of viral proteases, and/or suppressing and modulating the host’s immune response. Therefore, this review intends to discuss the most recent findings on the potential of phenolics to prevent SARS-CoV-2.

scholarly journals Preliminary Report on the Effect of Savanna Plants Leucaena leucocephala, Parkia platycephala and Senna alata against Eggs and Immature Stages of Trichostrongylid Nematodes In Vitro

Pathogens ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 986
Author(s):  
Benta Natânia Silva Figueiredo ◽  
Marcello Otake Sato ◽  
Laiane Teixeira Sousa Moura ◽  
Sandra Maria Botelho Mariano ◽  
Tarso da Costa Alvim ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Ellagic Acid ◽  
Leucaena Leucocephala ◽  
Phytochemical Analysis ◽  
Active Components ◽  
Active Metabolites ◽  
Egg Hatching ◽  
Senna Alata ◽  
Anthelmintic Effect

The current study evaluated the anthelmintic effect of different extracts of Leucaena leucocephala, Parkia platycephala, and Senna alata on trichostrongylid eggs and infective larvae and determined the potential active components of each plant. Dried and macerated plant material was concentrated using rotaevaporation to obtain the crude extract (CE), followed by solvent partitioning to obtain hexanic (HexE), acetatic (AcE), and butanolic (BuE) extracts used for phytochemical analysis and anthelmintic efficacy testing in vitro. All the crude and partitioned extracts tested showed inhibition activity in the hatching of trichostrongylid eggs. Larvicidal efficacy was observed at CE concentrations of 2.5, 5.0, and 7.5 mg/mL for P. platycephala and S. alata. However, L. leucocephala CE did not significantly reduce the number of living larvae in the tested concentrations. Chromatographic analysis revealed several active metabolites; gallic acid, ellagic acid, naringin, morin, and kaempferol on AcE of P. platycephala; gallic acid, rutin, and ellagic acid on BuE of P. platycephala; and gallic acid and naringin on BuE of L. leucocephala. The extracts of P. platycephala, L. leucocephala, and S. alata leaves showed egg hatching inhibition and larvicidal activity, probably produced by tannins and flavonoids, which may act alone or by synergism.

LED-activated pheophorbide a in ovarian cancer cells: Cytotoxicity and apoptosis induction

Laser Physics ◽  
2011 ◽  
Vol 21 (2) ◽  
pp. 423-426 ◽  
Author(s):  
L. Liu ◽  
C. S. Xu ◽  
X. S. Xia ◽  
A. W. N. Leung
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Apoptosis Induction ◽  
Pheophorbide A

scholarly journals Targeted microbubbles for ultrasound mediated gene transfection and apoptosis induction in ovarian cancer cells

2013 ◽  
Vol 20 (1) ◽  
pp. 171-179 ◽  
Author(s):  
Shufang Chang ◽  
Juan Guo ◽  
Jiangchuan Sun ◽  
Shenyin Zhu ◽  
Yu Yan ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Gene Transfection ◽  
Ovarian Cancer Cells ◽  
Apoptosis Induction ◽  
Targeted Microbubbles

Lachnellins A, B, C, D, and Naphthalene-l,3,8-triol, Biologically Active Compounds from a Lachnellula Species (Ascomycetes)

1996 ◽  
Vol 51 (7-8) ◽  
pp. 500-512 ◽  
Author(s):  
Martin Semar ◽  
Heidrun Anke ◽  
Wolf-Rüdiger Arendholz ◽  
Robert Veiten ◽  
Wolfgang Steglich
Keyword(s):  
Serum Albumin ◽  
Reactive Intermediates ◽  
Antimicrobial Activities ◽  
Malate Synthase ◽  
Biologically Active ◽  
Inhibiting Activity ◽  
Active Metabolites ◽  
Submerged Cultures ◽  
Active Compounds ◽  
Biologically Active Metabolites

Abstract In the course of our search for new biologically active metabolites, lachnellin A (1), a metabolite with high cytotoxic and antimicrobial activities, the structurally related lachnellins B, C and D (3, 4, 7), and naphthalene-1,3,8-triol (8), an inhibitor of malate synthase (EC 4.1.3.2), were isolated from submerged cultures of the ascomycete Lachnellula sp. A 32 -8 9 . The antimicrobial, cytotoxic and phytotoxic activities of lachnellin A depended on its reactivity and could be abolished by the addition of cysteine. The enzyme inhibiting activity of (8) was due to reactive intermediates during melanization and was no longer observed in the presence of serum albumin. In addition, rac-scytalone (9), (+)-trans-3,4-dihydro-3,4,8-trihy-droxy-1(2H)-naphthalenone (10). 2,5-dihydroxytoluene (11), and (R)-(-)-5-methylmellein (12) were obtained from the same source and biologically characterized.

scholarly journals Effect of Gallic acid and Myricetin on ovarian cancer models: a possible alternative antitumoral treatment

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Luis Varela-Rodríguez ◽  
Blanca Sánchez-Ramírez ◽  
Verónica Ivonne Hernández-Ramírez ◽  
Hugo Varela-Rodríguez ◽  
Rodrigo Daniel Castellanos-Mijangos ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gallic Acid ◽  
Cancer Models

Cytotoxic Activity of Gallic Acid and Myricetin against Ovarian Cancer Cells by Production of Reactive Oxygen Species

2021 ◽  
Vol 7 (1) ◽  
pp. 7
Author(s):  
Luis Varela-Rodríguez ◽  
Patricia Talamás-Rohana ◽  
Blanca Sánchez-Ramírez ◽  
Verónica Ivonne Hernández-Ramírez ◽  
Hugo Varela-Rodríguez
Keyword(s):  
Ovarian Cancer ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Gallic Acid ◽  
Therapeutic Potential ◽  
Chromatin Condensation ◽  
Vehicle Group ◽  
Ovarian Cancer Cells ◽  
Ros Generation ◽  
In Silico Studies

Some studies demonstrate that gallic acid (GA) and myricetin (MYR) isolated from Rhus trilobata provide the therapeutic activity of this plant against cancer. However, few reports demonstrate that both compounds could also have therapeutic potential in ovarian cancer. Therefore, evaluating the cytotoxic activity of GA and MYR against ovarian cancer cells and determining the possible action mechanism present are important. For this purpose, SKOV-3 cells (ovarian adenocarcinoma; HTB-77™, ATCC®) were cultivated according to the supplier’s instructions (37 °C and 5% CO2) to determine the biological activity of GA and MYR by confocal/transmission electron microscopy, PI-flow cytometry, H2DCF-DA, MTT, and Annexin-V assays. Possible molecular targets of the compounds were determined by the Similarity Ensemble approach. Results showed that GA and MYR treatments decreased the viability of SKOV-3 cells at 50 and 166 μg/mL, respectively (p ≤ 0.05, ANOVA vs. vehicle group). They also induced morphological changes (cytoplasmic reduction, nuclear chromatin condensation, cytoplasmic vesicles increment, polymerized actin, and stabilized tubulin), cell cycle arrest (GA: 8.3% G2/M and MYR: 78% G1), and apoptosis induction (GA: 18.9% and MYR: 8.1%), due to ROS generation (34 to 42%) for 24 h (p ≤ 0.05, ANOVA vs. vehicle group). In silico studies demonstrated that GA and MYR interact with carbonic anhydrase-IX and PI3K, respectively. In conclusion, GA and MYR show cytotoxic activity against SKOV-3 cells through ROS production, which modifies the cytoskeleton and induces apoptosis. Therefore, GA and MYR could be considered as base compounds for the development of new treatments in chemotherapy for ovarian cancer.

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