scholarly journals Genetic Features of Triticale–Wheat Hybrids with Vaviloid-Type Spike Branching

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 58
Author(s):  
Irina G. Adonina ◽  
Andrey B. Shcherban ◽  
Maremyana V. Zorina ◽  
Sabina P. Mehdiyeva ◽  
Ekaterina M. Timonova ◽  
...  
Keyword(s):  
Hexaploid Wheat ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Synthetic Wheat ◽  
Spike Morphology ◽  
Telocentric Chromosomes ◽  
Genetic Features ◽  
Unpaired Chromosome ◽  
Wheat Hybrids ◽  
Hybrid Lines

Vaviloid spike branching, also called sham ramification, is a typical trait of Triticum vavilovii Jakubz. and is characterized by a lengthening of the spikelet axis. In this article, we present the results of a study of three triticale–wheat hybrid lines with differences in terms of the manifestation of the vaviloid spike branching. Lines were obtained by crossing triticale with hexaploid wheat, T. aestivum var. velutinum. The parental triticale is a hybrid of synthetic wheat (T. durum × Ae. tauschii var. meyrei) with rye, S. cereale ssp. segetale. Line 857 has a karyotype corresponding to hexaploid wheat and has a spike morphology closest to normal, whereas Lines 808/1 and 844/4 are characterized by the greatest manifestation of vaviloid spike branching. In Lines 808/1 and 844/4, we found the substitution 2RL(2DL). The karyotypes of the latter lines differ in that a pair of telocentric chromosomes 2DS is detected in Line 808/1, and these telocentrics are fused into one unpaired chromosome in Line 844/4. Using molecular genetic analysis, we found a deletion of the wheat domestication gene Q located on 5AL in the three studied hybrid lines. The deletion is local since an analysis of the adjacent gene B1 showed the presence of this gene. We assume that the manifestation of vaviloid spike branching in two lines (808/1 and 844/4) is associated with a disturbance in the joint action of genes Q and AP2L2-2D, which is another important gene that determines spike morphology and is located on 2DL.

scholarly journals TOPICALITY OF FORENSIC MOLECULAR GENETIC EXAMINATION AND ISSUES REGARDING ITS PERFORMING

2018 ◽  
Vol 18 ◽  
pp. 256-263
Author(s):  
V. V. Topchiy
Keyword(s):  
Dna Analysis ◽  
Human Life ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Molecular Genetic Analysis ◽  
Present Stage ◽  
Law Enforcement Agencies ◽  
Biological Origin ◽  
Genetic Features ◽  
Genetic Examination

Modern progress in forensic molecular genetic examination allow to obtain information about a particular person using traces variety of biological origin especially while committing grave crimes against human life and health, that are usually found at the scene and belong to a human body. A significant advantage of this method under crime investigation is precisely the safe exclusion of suspected persons not involved in the commission of a crime, in identifying those who committed a crime with a high probability level. At the present stage of forensic molecular genetic examination development there are significant gaps in legislation that are solved by adopting relevant normative and legal acts and improving existing ones. Effective method for of DNA analysis development is the creation of appropriate bases of genetic features of a person. However, the legislative consolidation of this process should take place in the context of respecting and protecting personal rights. However, terms of performing molecular genetic examination significantly exceed the terms of pre-trial investigation. This problem can be solved by expanding network of laboratories that perform such examination. Despite presence of a small number of problems, it is possible to affirm that DNA analysis is the most effective and reliable of all known methods of person identification at the present stage. At present, expert molecular genetic analysis develops not only as a section of molecular genetic research but also as a complete element of criminalistic knowledge that is aimed at investigating and disclosing crimes. Therefore, implementation of molecular genetic research methods into the practice of law enforcement agencies in Ukraine will significantly increase investigation effectiveness of many serious crimes against person.

scholarly journals Role of AGTR1 A/C polymorphism in the development of atrial fibrillation

2017 ◽  
Vol 89 (9) ◽  
pp. 48-52
Author(s):  
A V Kuskaeva ◽  
S Yu Nikulina ◽  
A A Chernova ◽  
N V Aksyutina ◽  
A P Kuskaev ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Molecular Genetic ◽  
Geographical Location ◽  
Risk Groups ◽  
Molecular Genetic Analysis ◽  
Climatic Conditions ◽  
Control Group ◽  
Study Group ◽  
Genetic Features ◽  
Electrocardiogram Monitoring

Aim. To investigate the AGTR1 A/C polymorphism associated with atrial fibrillation (AF) to form risk groups among patients who are prone to this disease. Subjects and methods. 90 probands with a confirmed diagnosis of AF and their 144 first-, second-, and third-degree relatives were examined. These families made up a study group. A control group was formed of 100 apparently healthy individuals without a history of cardiovascular diseases. Collection of medical history data and complaints, electrocardiography, electrocardiogram monitoring, as well as molecular genetic analysis, thyroid hormone tests were done in all the patients. Results. No statistically significant data on the correlation between the AGTR1 A/C polymorphism and the development of AF were obtained in any patient subgroup. The obtained results can be due to the genetic features of a Siberian population, which are dependent on climatic conditions and geographical location, and confirm that AF is a heterogeneous disease. Conclusion. There were no statistically significant differences between the patients in the study group and those in the control group. Our findings suggest the heterogeneity of AF and confirm its multifactorial nature.

NIMG-76. DISCRETE LOWER-GRADE GLIOMA (DLGG) VERSUS INFILTRATIVE LOWER-GRADE GLIOMA (ILGG): CORRELATION OF RADIOLOGICAL CHARACTERISTICS WITH CLINICAL OUTCOMES

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi146-vi147
Author(s):  
Ahsan Ali Khan ◽  
Mohammad Hamza Bajwa ◽  
Noman Khan ◽  
Muhammad Usman Khalid ◽  
Fatima Mubarak ◽  
...  
Keyword(s):  
Clinical Outcomes ◽  
Molecular Genetic ◽  
Tumor Grade ◽  
Molecular Genetic Analysis ◽  
Vital Role ◽  
Lower Grade ◽  
Radiographic Appearance ◽  
Patient Demographics ◽  
Lower Grade Glioma ◽  
Genetic Features

Abstract INTRODUCTION Lower grade gliomas encompass grade 2 and 3 tumors. However, this term is more generalized and does not include the spectrum of radiological and tumor morphological patterns seen. Here we have established two distinct patterns of radiographic appearance seen within lower grade gliomas: ILGG and DLGG. Imaging plays a vital role in diagnosis, surveillance, characterization, and monitoring of intracranial tumors. Of particular importance is the differentiation of tumor features to reliably predict malignancy, tumor grade, possible molecular or genetic features, disease progression and recurrence, potential for malignant transformation, and postoperative outcomes. Our study will look at these radiographic characteristics of diffuse and infiltrating lower grade gliomas and discuss their predictive value. Understanding the distinct nature of these varieties of LGG will help us in surgical decision-making, prognostication, biopsy target and precision medicine. METHODS Pre-operative and post-operative MRI images of Grade 2 and 3 tumors were identified and analyzed in order to extract radiographic data, and correlated with patient demographics, clinical outcomes, extent of surgical resection, and molecular genetic analysis. RESULTS Out of 35 patients evaluated, 22 (62.9%) were labeled ILGGs and 13 (37.1%) were deemed DLGGs according to the pre-defined criteria. T2 habitat was higher in ILGG (mean = 2162) than DLGG (mean = 1482) as well as size, in cm (6.02 vs. 4.92). ADC habitat, lesion ADC, and percentage of the lesion that showed contrast-enhancement were similar. T2-FLAIR mismatch was significantly higher in ILGG (p = 0.02). Post-operative KPS scores were significantly higher in the DLGG group (p = 0.03). CONCLUSION T2-FLAIR mismatch can be a significant classifier for lower-grade gliomas. Our study shows there are differences in tumor morphology of diffuse and infiltrative lower-grade gliomas which can be correlated to outcomes after surgery. *Indicates corresponding author.

scholarly journals Follicular Lymphoma Diagnostic Caveats and Updates

2018 ◽  
Vol 142 (11) ◽  
pp. 1330-1340 ◽  
Author(s):  
Sarah M. Choi ◽  
Bryan L. Betz ◽  
Anamarija M. Perry
Keyword(s):  
Follicular Lymphoma ◽  
Tissue Sample ◽  
Molecular Genetic ◽  
B Cell Lymphoma ◽  
Molecular Genetic Analysis ◽  
Genetic Studies ◽  
Diagnostic Strategies ◽  
General Pathology ◽  
Genetic Features ◽  
Precise Diagnosis

Context.— Follicular lymphoma is a common small B-cell lymphoma, likely to be encountered by any practicing pathologist, regardless of specialty. Although the features of typical follicular lymphoma are well known and in most instances easily identifiable, there are lesser-appreciated morphologic appearances that can raise alternative diagnostic possibilities. The limited tissue available in core needle biopsies can make it additionally challenging to thoroughly evaluate those features in the context of architecture. Furthermore, ancillary testing including immunohistochemistry and molecular/genetic analysis do not always show classic findings and may pose additional challenges to interpretation. Objectives.— To review the morphologic features of follicular lymphoma with a discussion of morphologic variants and mimics; to discuss pitfalls of ancillary testing and provide the practicing pathologist with an appropriate context for interpretation of immunohistochemical and molecular/genetic studies when follicular lymphoma is part of the differential diagnosis; and to propose diagnostic strategies when there is limited tissue for evaluation. Data Sources.— We used examples of follicular lymphoma from our institution as well as a review of the literature, with a focus on the diagnostic aspects that are broadly relevant to a general pathology practice. Conclusions.— Follicular lymphoma can occasionally present with atypical morphologic, immunohistochemical, or molecular/genetic features. In particular, those findings can be difficult to interpret in the setting of a limited tissue sample. Awareness of those possibilities will help guide the pathologist to a more accurate and precise diagnosis.

Molecular Characterization and Genetic Diversity Study in F3 Population of Rice

2013 ◽  
Vol 20 (1-2) ◽  
pp. 1-8
Author(s):  
MM Rahman ◽  
L Rahman ◽  
SN Begum ◽  
F Nur
Keyword(s):  
Genetic Distance ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Polymorphic Loci ◽  
Rice Genotypes ◽  
High Level ◽  
Genetic Diversity Study ◽  
Diversity Study

Random Amplified Polymorphic DNA (RAPD) assay was initiated for molecular genetic analysis among 13 F3 rice lines and their parents. Four out of 15 decamer random primers were used to amplify genomic DNA and the primers yielded a total of 41 RAPD markers of which 37 were considered as polymorphic with a mean of 9.25 bands per primer. The percentage of polymorphic loci was 90.24. The highest percentage of polymorphic loci (14.63) and gene diversity (0.0714) was observed in 05-6 F3 line and the lowest polymorphic loci (0.00) and gene diversity (0.00) was found in 05-12 and 05-15 F3 lines. So, relatively high level of genetic variation was found in 05-6 F3 line and it was genetically more diverse compared to others. The average co-efficient of gene differentiation (GST) and gene flow (Nm) values across all the loci were 0.8689 and 0.0755, respectively. The UPGMA dendrogram based on the Nei’s genetic distance differentiated the rice genotypes into two main clusters: PNR-519, 05-19, 05-14, 05-12 and 05-17 grouped in cluster 1. On the other hand, Baradhan, 05-9, 05-13, 05-11, 05-5, 05-6, 05-1, 05-4, 05-15 and 05-25 were grouped in cluster 2. The highest genetic distance (0.586) was found between 05-4 and 05-17 F3 lines and they remain in different cluster.DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16839 Progress. Agric. 20(1 & 2): 1 – 8, 2009

Diagnosis and Management of Cardiomyopathies – A Focus on Genetics, Cardiac Magnetic Resonance and Clinical Features

2011 ◽  
Vol 7 (3) ◽  
pp. 225
Author(s):  
Gianfranco Sinagra ◽  
Michele Moretti ◽  
Giancarlo Vitrella ◽  
Marco Merlo ◽  
Rossana Bussani ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Cardiac Magnetic Resonance ◽  
Magnetic Resonance ◽  
Imaging Techniques ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Routine Clinical Practice ◽  
Clinical Approach ◽  
Diagnosis And Management ◽  
Made In

In recent years, outstanding progress has been made in the diagnosis and treatment of cardiomyopathies. Genetics is emerging as a primary point in the diagnosis and management of these diseases. However, molecular genetic analyses are not yet included in routine clinical practice, mainly because of their elevated costs and execution time. A patient-based and patient-oriented clinical approach, coupled with new imaging techniques such as cardiac magnetic resonance, can be of great help in selecting patients for molecular genetic analysis and is crucial for a better characterisation of these diseases. This article will specifically address clinical, magnetic resonance and genetic aspects of the diagnosis and management of cardiomyopathies.

scholarly journals MOLECULAR GENETIC ANALYSIS OF THE DILUTE-SHORT EAR (d-se) REGION OF THE MOUSE

Genetics ◽  
1986 ◽  
Vol 112 (2) ◽  
pp. 321-342
Author(s):  
Eugene M Rinchik ◽  
Liane B Russell ◽  
Neal G Copeland ◽  
Nancy A Jenkins
Keyword(s):  
Physical Map ◽  
Leukemia Virus ◽  
Molecular Genetic ◽  
Break Point ◽  
Virus Genome ◽  
Molecular Genetic Analysis ◽  
Chromosome 9 ◽  
Genetic Complementation ◽  
Induced Mutations ◽  
Radiation Induced

ABSTRACT Genes of the dilute-short ear (d-se) region of mouse chromosome 9 comprise an array of loci important to the normal development of the animal. Over 200 spontaneous, chemically induced and radiation-induced mutations at these loci have been identified, making it one of the most genetically well-characterized regions of the mouse. Molecular analysis of this region has recently become feasible by the identification of a dilute mutation that was induced by integration of an ecotropic murine leukemia virus genome. Several unique sequence cellular DNA probes flanking this provirus have now been identified and used to investigate the organization of wild-type chromosomes and chromosomes with radiation-induced d-se region mutations. As expected, several of these mutations are associated with deletions, and, in general, the molecular and genetic complementation maps of these mutants are concordant. Furthermore, a deletion break-point fusion fragment has been identified and has been used to orient the physical map of the d-se region with respect to the genetic complementation map. These experiments provide important initial steps for analyzing this developmentally important region at the molecular level, as well as for studying in detail how a diverse group of mutagens acts on the mammalian germline.

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