scholarly journals Development of Systems for the Production of Plant-Derived Biopharmaceuticals

Plants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 30 ◽  
Author(s):  
Ki-Beom Moon ◽  
Ji-Sun Park ◽  
Youn-Il Park ◽  
In-Ja Song ◽  
Hyo-Jun Lee ◽  
...  
Keyword(s):  
Open Field ◽  
Recombinant Proteins ◽  
Food Crops ◽  
Expression Systems ◽  
Minimally Processed ◽  
Vertical Farming ◽  
Rapid Production ◽  
Hydroponic Systems ◽  
Whole Plants ◽  
Intrinsic Safety

Over the last several decades, plants have been developed as a platform for the production of useful recombinant proteins due to a number of advantages, including rapid production and scalability, the ability to produce unique glycoforms, and the intrinsic safety of food crops. The expression methods used to produce target proteins are divided into stable and transient systems depending on applications that use whole plants or minimally processed forms. In the early stages of research, stable expression systems were mostly used; however, in recent years, transient expression systems have been preferred. The production of the plant itself, which produces recombinant proteins, is currently divided into two major approaches, open-field cultivation and closed-indoor systems. The latter encompasses such regimes as greenhouses, vertical farming units, cell bioreactors, and hydroponic systems. Various aspects of each system will be discussed in this review, which focuses mainly on practical examples and commercially feasible approaches.

scholarly journals The multifunctionality of expression systems in Bacillus subtilis: Emerging devices for the production of recombinant proteins

2021 ◽  
pp. 153537022110301
Author(s):  
Caio Coutinho de Souza ◽  
Jander Matos Guimarães ◽  
Soraya dos Santos Pereira ◽  
Luis André Morais Mariúba
Keyword(s):  
Bacillus Subtilis ◽  
Recombinant Proteins ◽  
Large Scale ◽  
Recombinant Expression ◽  
Academic Research ◽  
Future Research ◽  
Expression Systems ◽  
Bioactive Substances ◽  
Exogenous Dna ◽  
Chemical Inducers

Bacillus subtilis is a successful host for producing recombinant proteins. Its GRAS (generally recognized as safe) status and its remarkable innate ability to absorb and incorporate exogenous DNA into its genome make this organism an ideal platform for the heterologous expression of bioactive substances. The factors that corroborate its value can be attributed to the scientific knowledge obtained from decades of study regarding its biology that has fostered the development of several genetic engineering strategies, such as the use of different plasmids, engineering of constitutive or double promoters, chemical inducers, systems of self-inducing expression with or without a secretion system that uses a signal peptide, and so on. Tools that enrich the technological arsenal of this expression platform improve the efficiency and reduce the costs of production of proteins of biotechnological importance. Therefore, this review aims to highlight the major advances involving recombinant expression systems developed in B. subtilis, thus sustaining the generation of knowledge and its application in future research. It was verified that this bacterium is a model in constant demand and studies of the expression of recombinant proteins on a large scale are increasing in number. As such, it represents a powerful bacterial host for academic research and industrial purposes.

scholarly journals Different expression systems for production of recombinant proteins in Saccharomyces cerevisiae

2012 ◽  
Vol 109 (5) ◽  
pp. 1259-1268 ◽  
Author(s):  
Zihe Liu ◽  
Keith E.J. Tyo ◽  
José L. Martínez ◽  
Dina Petranovic ◽  
Jens Nielsen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Recombinant Proteins ◽  
Expression Systems

scholarly journals N- and O-Glycosylation Pathways in the Microalgae Polyphyletic Group

2020 ◽  
Vol 11 ◽  
Author(s):  
Elodie Mathieu-Rivet ◽  
Narimane Mati-Baouche ◽  
Marie-Laure Walet-Balieu ◽  
Patrice Lerouge ◽  
Muriel Bardor
Keyword(s):  
Recombinant Proteins ◽  
Metabolic Pathways ◽  
Phylogenetic Diversity ◽  
State Of The Art ◽  
Protein Glycosylation ◽  
Expression Systems ◽  
Post Translational Modification ◽  
Photosynthetic Organisms ◽  
Biological Features ◽  
Heterologous Expression Systems

The term microalga refers to various unicellular and photosynthetic organisms representing a polyphyletic group. It gathers numerous species, which can be found in cyanobacteria (i.e., Arthrospira) as well as in distinct eukaryotic groups, such as Chlorophytes (i.e., Chlamydomonas or Chlorella) and Heterokonts (i.e., diatoms). This phylogenetic diversity results in an extraordinary variety of metabolic pathways, offering large possibilities for the production of natural compounds like pigments or lipids that can explain the ever-growing interest of industrials for these organisms since the middle of the last century. More recently, several species have received particular attention as biofactories for the production of recombinant proteins. Indeed, microalgae are easy to grow, safe and cheap making them attractive alternatives as heterologous expression systems. In this last scope of applications, the glycosylation capacity of these organisms must be considered as this post-translational modification of proteins impacts their structural and biological features. Although these mechanisms are well known in various Eukaryotes like mammals, plants or insects, only a few studies have been undertaken for the investigation of the protein glycosylation in microalgae. Recently, significant progresses have been made especially regarding protein N-glycosylation, while O-glycosylation remain poorly known. This review aims at summarizing the recent data in order to assess the state-of-the art knowledge in glycosylation processing in microalgae.

scholarly journals Indoor Farming Marjoram Production—Quality, Resource Efficiency, and Potential of Application

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1769
Author(s):  
Sabine Wittmann ◽  
Ivonne Jüttner ◽  
Heike Mempel
Keyword(s):  
Open Field ◽  
Food Industry ◽  
Growth Period ◽  
Raw Material ◽  
Growth And Yield ◽  
Simultaneous Increase ◽  
Layer System ◽  
High Resource ◽  
Vertical Farming ◽  
Field Production

Indoor vertical farming offers great opportunities regarding a sustainable and consistent production of high-quality herbs and raw materials all year round for the perfume, chemical, or food industry. Cultivation takes place in an enclosed structure, operating predominantly independent from external conditions in multi-layer systems equipped with artificial lighting, enabling extremely high resource use efficiencies with a simultaneous increase in yield. On the other hand, field production in terms of plant quality and harvesting times is highly influenced by environmental conditions, making it difficult to maintain homogenous raw material qualities throughout the year. To show how different light qualities affect the overall efficiency and quality of Origanum majorana grown in an indoor farm, the resource consumption, yield, and cultivation time as well as the essential oil quantity was analyzed, and the efficiencies in terms of energy and land use efficiency calculated. The experimental setup clearly demonstrated that the yield regarding fresh as well as dry matter and oil content was comparable to one square meter of open field production. Based on this, the multi-layer system and the noticeable lowered growth period result in a significantly higher area efficiency compared to the open field, leading to a potential increase of annual yields of dried leave weight and oil contents by up to 21 times. It was also shown that a white spectrum (W) showed similar influence on plant growth and yield as a spectrum consisting of blue and red (B/R). Nevertheless, the LED treatment W did show higher light use efficiencies as well as a better working conditions inside the cultivation chamber. By an integration of indoor vertical farming into existing industrial processes, new and innovative opportunities for a flexible and low-risk supply chain seem feasible and according to German food industry meet the interests of existing stakeholders.

scholarly journals Evaluation of Sedative and Hypnotic Activity of Ethanolic Extract ofScoparia dulcisLinn.

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Md. Moniruzzaman ◽  
Md. Atikur Rahman ◽  
Afia Ferdous
Keyword(s):  
Open Field ◽  
Scientific Information ◽  
Ethanolic Extract ◽  
Perennial Herb ◽  
Reference Drug ◽  
Scoparia Dulcis ◽  
Hole Board ◽  
Thiopental Sodium ◽  
Whole Plants ◽  
Hypnotic Activity

Scoparia dulcisLinn. (SD) is a perennial herb that has been well studied for its antioxidant, anti-inflammatory, antidiabetic, and hepatoprotective effects. However, scientific information on SD regarding the neuropharmacological effect is limited. This study evaluated the sedative and hypnotic effect of the ethanolic extract of whole plants ofScoparia dulcis(EESD). For this purpose, the whole plants ofS. dulciswere extracted with ethanol following maceration process and tested for the presence of phytochemical constituents. The sedative and hypnotic activity were then investigated using hole cross, open field, hole-board, rota-rod, and thiopental sodium-induced sleeping time determination tests in mice at the doses of 50, 100, and 200 mg/kg of EESD. Diazepam at the dose of 1 mg/kg was used as a reference drug in all the experiments. We found that EESD produced a significant dose-dependent inhibition of locomotor activity of mice both in hole cross and open field tests (P<0.05). Besides, it also decreased rota-rod performances and the number of head dips in hole-board test. Furthermore, EESD significantly decreased the induction time to sleep and prolonged the duration of sleeping, induced by thiopental sodium. Taken together, our study suggests that EESD may possess sedative principles with potent hypnotic properties.

scholarly journals Comparative analysis of Aquaponics and Hydroponic systems

2021 ◽  
Vol 5 ◽  
pp. 31-43
Author(s):  
Vilma Petkova ◽  
Kristina Krusteva
Keyword(s):  
Life Cycle ◽  
Comparative Analysis ◽  
Middle Ages ◽  
Chemical Elements ◽  
Arable Land ◽  
Urban Environments ◽  
Controlled Environment ◽  
Food Crops ◽  
Hydroponic System ◽  
Hydroponic Systems

This article presents an analysis of the Aquaponics and Hydroponic systems. The principles of action have been known since the Middle Ages, but in present times they have developed due to an increase in the planet’s population, food and water scarcity, arable land, and environmental pollution. The Hydroponic system is groundless cultivation of food crops, mainly vegetables in a controlled environment. In Aquaponic system the nutrients circulates while combining the life cycle of suitable plants and aquatic fishes. This ensures the functioning of a system that resembles the circles of biogenic chemical elements in nature. Both systems are highly efficient and ecological cleaning, making them particularly suitable for use in urban environments.

scholarly journals Improving plant transient expression through the rational design of synthetic 5′ and 3′ untranslated regions

Plant Methods ◽  
2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Hadrien Peyret ◽  
James K. M. Brown ◽  
George P. Lomonossoff
Keyword(s):  
Recombinant Protein ◽  
Transient Expression ◽  
Recombinant Proteins ◽  
Rational Design ◽  
Critical Role ◽  
Regulatory Elements ◽  
Plant Viruses ◽  
Untranslated Regions ◽  
Expression Vectors ◽  
Expression Systems

Abstract Background The growing field of plant molecular farming relies on expression vectors that allow high yields of recombinant proteins to be produced through transient gene expression. While numerous expression vectors currently exist for this purpose, there are very few examples of systematic efforts to improve upon these. Moreover, the current generation of expression systems makes use of naturally-occurring regulatory elements, typically selected from plant viruses, to maximise yields. This study aims to use rational design to generate synthetic sequences that can rival existing ones. Results In this work, we present the rational design of novel synthetic 5′ and 3′ untranslated regions (UTRs) which can be used in various combinations to modulate accumulation levels of transiently-expressed recombinant proteins. Using the pEAQ-HT expression vector as a point of comparison, we show that pre-existing expression systems can be improved by the deployment of rationally designed synthetic UTRs. Notably, we show that a suite of short, synthetic 5′UTRs behave as expression enhancers that outperform the HT 5′UTR present in the CPMV-HT expression system. Furthermore, we confirm the critical role played by the 3′UTR of cowpea mosaic virus RNA-2 in the performance of the CPMV-HT system. Finally, we use the knowledge obtained from these results to develop novel expression vectors (named pHRE and pHREAC) that equal or outperform pEAQ-HT in terms of recombinant protein yield. These new vectors are also domesticated for the use of certain Type IIS restriction enzymes, which allows for quicker cloning and straightforward assessment of different combinations of UTRs. Conclusions We have shown that it is possible to rationally design a suite of expression modulators in the form of synthetic UTRs. We have created novel expression vectors that allow very high levels of recombinant protein expression in a transient expression context. This will have important consequences for future efforts to develop ever-better plant transient overexpression vectors for research or industrial applications.

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