Doxorubicin-Conjugated Iron Oxide Nanoparticles Synthesized by Laser Pyrolysis: In Vitro Study on Human Breast Cancer Cells

Even today, breast cancer remains a global public problem, with a high mortality rate among women. Nanoparticle (NP) based systems are developed to enhance drug delivery, reducing the toxic effect of medicine molecules. By using iron oxide nanoparticles for cancer treatment, several advantages were highlighted: the ability to target specific locations derived from their magnetic properties and reduced side effects. The aim of this study was to examine on breast cancer cell line the anticancer potential of γ-Fe2O3 NPs loaded with doxorubicin (DOX) and stabilized with carboxymethylcellulose sodium (CMCNa). The γ-Fe2O3 NPs were synthesized by laser pyrolysis technique and their nanometric size and crystallinity were confirmed by X-ray diffraction and transmission electron microscopy. The loading efficiency was estimated by using absorption and fluorescence spectroscopy. The DOX conjugated//CMCNa coated γ-Fe2O3 NPs proved through the biological studies to have a good anticancer effect through the inhibition of tumoral cell proliferation, disruption of the cellular membrane, induction of cell death and reduced effects on normal breast cells. Our data showed that DOX cytotoxicity increases significantly when conjugated with ɣ-Fe2O3 and ɣ-Fe2O3_CMCNa, a 50% reduction of cancer cell viability was obtained with a concentration around 0.1 µg/mL.

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Magnetic iron oxide nanoparticles mediated gene therapy for breast cancer — An In Vitro study

Journal of Huazhong University of Science and Technology [Medical Sciences] ◽

10.1007/s11596-006-0628-y ◽

2006 ◽

Vol 26 (6) ◽

pp. 728-730 ◽

Cited By ~ 2

Author(s):

Weizhong Wei ◽

Chunfang Xu ◽

Hua Wu

Keyword(s):

Breast Cancer ◽

Gene Therapy ◽

Iron Oxide ◽

Iron Oxide Nanoparticles ◽

In Vitro Study ◽

Vitro Study ◽

Oxide Nanoparticles ◽

Magnetic Iron Oxide Nanoparticles ◽

Magnetic Iron Oxide

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Polymer Coated Iron Nanoparticles: Radiolabeling & In Vitro Studies

Current Radiopharmaceuticals ◽

10.2174/1874471013666200430094113 ◽

2020 ◽

Vol 13 ◽

Author(s):

Selin Yılmaz ◽

Çiğdem İçhedef ◽

Kadriye Buşra Karatay ◽

Serap Teksöz

Keyword(s):

Breast Cancer ◽

Drug Delivery ◽

Iron Oxide ◽

Cancer Cells ◽

Iron Oxide Nanoparticles ◽

Breast Cancer Cells ◽

Cancer Drug ◽

Oxide Nanoparticles ◽

Sem Images

Backgorund: Superparamagnetic iron oxide nanoparticles (SPIONs) have been extensively used for targeted drug delivery systems due to their unique magnetic properties. Objective: In this study, it’s aimed to develop a novel targeted 99mTc radiolabeled polymeric drug delivery system for Gemcitabine (GEM). Methods: Gemcitabine, an anticancer agent, was encapsulated into polymer nanoparticles (PLGA) together with iron oxide nanoparticles via double emulsion technique and then labeled with 99mTc. SPIONs were synthesized by reduction–coprecipitation method and encapsulated with oleic acid for surface modification. Size distribution and the morphology of the synthesized nanoparticles were caharacterized by dynamic light scattering(DLS)and scanning electron microscopy(SEM), respectively. Radiolabeling yield of SPION-PLGAGEM nanoparticles were determined via Thin Layer Radio Chromatography (TLRC). Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells in vitro. Results: SEM images displayed that the average size of the drug-free nanoparticles was 40 nm and the size of the drug-loaded nanoparticles was 50 nm. The diameter of nanoparticles were determined as 366.6 nm by DLS, while zeta potential was found as-29 mV. SPION was successfully coated with PLGA, which was confirmed by FTIR. GEM encapsulation efficiency of SPION-PLGA was calculated as 4±0.16 % by means of HPLC. Radiolabeling yield of SPION-PLGA-GEM nanoparticles were determined as 97.8±1.75 % via TLRC. Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells. SPION-PLGA-GEM showed high uptake on MCF-7, whilst incorporation rate was increased for both cell lines which external magnetic field application. Conclusion: 99mTc labeled SPION-PLGA nanoparticles loaded with GEM may overcome some of the obstacles in anti-cancer drug delivery because of their appropriate size, non-toxic, and supermagnetic characteristics.

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Development of a T cell-targeted siRNA delivery system against HIV-1 using modified superparamagnetic iron oxide nanoparticles: An in vitro study

Journal of Pharmaceutical Sciences ◽

10.1016/j.xphs.2021.10.018 ◽

2021 ◽

Author(s):

Sara Kamalzare ◽

Vahid Iranpur Mobarakeh ◽

Farnaz Sadat Mirzazadeh Tekie ◽

Maliheh Hajiramezanali ◽

Farhad Riazi-Rad ◽

...

Keyword(s):

Iron Oxide ◽

T Cell ◽

Iron Oxide Nanoparticles ◽

Sirna Delivery ◽

Superparamagnetic Iron Oxide ◽

In Vitro Study ◽

Oxide Nanoparticles ◽

Hiv 1 ◽

Sirna Delivery System

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Analysis on Homoeopathic Preparation of Asterias Rubens for Evaluating Anti Breast Cancer Cell Line using Similia Principle

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl4.4075 ◽

2020 ◽

Vol 11 (SPL4) ◽

pp. 805-808

Author(s):

Ravikumar Raju ◽

Teja ◽

Sravanathi P ◽

Muthu Babu K

Keyword(s):

Breast Cancer ◽

Cell Line ◽

Cancer Cell ◽

Cancer Cell Line ◽

In Vitro Study ◽

In Vivo Studies ◽

Asterias Rubens ◽

Mcf 7

Breast cancer is the subsequent foremost reason of cancer death in a woman and ranks as the primary foremost reason of death in India. In its conduct, several measures and recommendation are considered. Homoeopathic medicines are one of the part of a corresponding, and another medicine is utilized for the treatment of cancer. The main purpose of the investigation is to evaluate the anticancer action of homoeopathic arrangements of Asterias rubens on the basis of the similia principle. We directed an in vitro study using MTT assay to control the result of ultra diluted homoeopathic preparation in contradiction of two human breast glandular cancer cell lines(MCF-7 and MDA-MD- 231), frequently used for the breast cancer treatment, by testing the feasibility of breast cancer (MCF-7 and MDA-MD-231) cell line, with various attenuations of Asterias rubens at 24 hrs. Multiple comparisons between tested reagents at different concentrations confirmed the significance of the said results. At a dilution of 1:25 6CH and 30CH potency shown superior activity on MCF-7 and no such significant changes on MDA-MD-231 at any dilutions As it fails to offer estrogen receptor(ER) Also progesterone receptor (PR) expression, and also HER2 (human epidermal development variable receptor2) so continuously a triple-negative breast cancer it will be a hostility manifestation for breast cancer with restricted medicine choices. However, further potency needs to be tested. These preliminary significant results warrant further in vitro and in vivo studies to estimate the possible of Asterias rubens a medicine to treat breast cancer.

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