Fabrication and Properties of Electrospun Collagen Tubular Scaffold Crosslinked by Physical and Chemical Treatments

Tissue engineered scaffold was regarded as a promising approach instead of the autograft. In this study, small diameter electrospun collagen tubular scaffold with random continuous smooth nanofibers was successfully fabricated. However, the dissolution of collagen in concentrated aqueous (conc. aq.) acetic acid caused to the serious denaturation of collagen. A novel method ammonia treatment here was adopted which recovered the collagen triple helix structure according to the analysis of IR spectra. Further dehydrothermal (DHT) and glutaraldehyde (GTA) treatments were applied to introduce the crosslinks to improve the properties of collagen tube. The nanofibrous structure of collagen tube in a wet state was preserved by the crosslinking treatments. Swelling ratio and weight loss decreased by at least two times compared to those of the untreated collagen tube. Moreover, tensile strength was significantly enhanced by DHT treatment (about 0.0076 cN/dTex) and by GTA treatment (about 0.075 cN/dTex). In addition, the surface of crosslinked collagen tube kept the hydrophilic property. These results suggest that DHT and GTA treatments can be utilized to improve the properties of electrospun collagen tube which could become a suitable candidate for tissue engineered scaffold.

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Faculty Opinions recommendation of Crystal structure of the collagen triple helix model [(Pro-Pro-Gly)(10)](3).

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1004876.56455 ◽

2002 ◽

Author(s):

Patricia C Weber

Keyword(s):

Crystal Structure ◽

Triple Helix ◽

Collagen Triple Helix ◽

Triple Helix Model

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Use of biocontrol agents in combination with physical and chemical treatments: efficacy assessment

Stewart Postharvest Review ◽

10.2212/spr.2014.1.2 ◽

2014 ◽

Vol 10 (1) ◽

pp. 1-4 ◽

Cited By ~ 3

Keyword(s):

Biocontrol Agents ◽

Chemical Treatments ◽

Efficacy Assessment ◽

Physical And Chemical

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The Role of Collagen Triple Helix Repeat-Containing 1 Protein (CTHRC1) in Rheumatoid Arthritis

International Journal of Molecular Sciences ◽

10.3390/ijms22052426 ◽

2021 ◽

Vol 22 (5) ◽

pp. 2426

Author(s):

Askhat Myngbay ◽

Limara Manarbek ◽

Steve Ludbrook ◽

Jeannette Kunz

Keyword(s):

Rheumatoid Arthritis ◽

Drug Targets ◽

Triple Helix ◽

Cancer Metastasis ◽

Bone Erosion ◽

Cartilage Destruction ◽

Patient Treatment ◽

Collagen Triple Helix ◽

Potential Biomarker

Rheumatoid arthritis (RA) is a chronic autoimmune disease causing inflammation of joints, cartilage destruction and bone erosion. Biomarkers and new drug targets are actively sought and progressed to improve available options for patient treatment. The Collagen Triple Helix Repeat Containing 1 protein (CTHRC1) may have an important role as a biomarker for rheumatoid arthritis, as CTHRC1 protein concentration is significantly elevated in the peripheral blood of rheumatoid arthritis patients compared to osteoarthritis (OA) patients and healthy individuals. CTHRC1 is a secreted glycoprotein that promotes cell migration and has been implicated in arterial tissue-repair processes. Furthermore, high CTHRC1 expression is observed in many types of cancer and is associated with cancer metastasis to the bone and poor patient prognosis. However, the function of CTHRC1 in RA is still largely undefined. The aim of this review is to summarize recent findings on the role of CTHRC1 as a potential biomarker and pathogenic driver of RA progression. We will discuss emerging evidence linking CTHRC1 to the pathogenic behavior of fibroblast-like synoviocytes and to cartilage and bone erosion through modulation of the balance between bone resorption and repair.

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Modulating the collagen triple helix formation by switching: Positioning effects of depsi-defects on the assembly of [Gly-Pro-Pro]7 collagen mimetic peptides

European Polymer Journal ◽

10.1016/j.eurpolymj.2018.12.045 ◽

2019 ◽

Vol 112 ◽

pp. 301-305 ◽

Cited By ~ 3

Author(s):

Matthias Röber ◽

Sophie Laroque ◽

Sandra A. Lopez ◽

Thomas Scheibel ◽

Hans G. Börner

Keyword(s):

Triple Helix ◽

Collagen Triple Helix ◽

Helix Formation ◽

Mimetic Peptides ◽

Triple Helix Formation ◽

Collagen Mimetic Peptides

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Effects of physical and chemical treatments upon biophysical properties and micro-relief of human skin

Archives of Dermatological Research ◽

10.1007/s00403-008-0849-y ◽

2008 ◽

Vol 300 (5) ◽

pp. 243-251 ◽

Cited By ~ 14

Author(s):

Nicolas Atrux-Tallau ◽

Ngoc T. T. Huynh ◽

Laurie Gardette ◽

Cyril Pailler-Mattéi ◽

Hassan Zahouani ◽

...

Keyword(s):

Human Skin ◽

Biophysical Properties ◽

Chemical Treatments ◽

Physical And Chemical

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Monitoring of End-Mill Temperature with Infrared Thermography and Wireless Tool Holder System

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1017.624 ◽

2014 ◽

Vol 1017 ◽

pp. 624-629 ◽

Cited By ~ 1

Author(s):

Masatoshi Shindou ◽

Ryo Matsuda ◽

Tatsuya Furuki ◽

Toshiki Hirogaki ◽

Eiichi Aoyama

Keyword(s):

End Milling ◽

Small Diameter ◽

Sampling Time ◽

End Mill ◽

Monitoring Tool ◽

Tool Temperature ◽

Wireless System ◽

Response Measurement ◽

Tool Holder ◽

Novel Method

Nowadays, infrared thermographic technology has been attracting attention in various industrial fields. We therefore focus on it as a novel method for monitoring tool temperature to improve end-milling conditions for difficult-to-cut materials. However, a problem has emerged; it is difficult to measure the tool temperature when there is a coolant because the coolant prevents monitoring of the surface of the end-mill tool. Thus, we developed a wireless tool holder system equipped with a thermocouple in the end mill to monitor the tool temperature under coolant conditions. In this report, we compared the temperature measured by infrared thermographic imagery with that measured by a wireless tool holder system when end milling the stainless steel under dry coolant conditions. The thermocouple, which has a small diameter of 0.12 mm, was used to ensure high response measurement in the proposed wireless tool holder. We obtained the tool temperatures by infrared thermographic imagery and by wireless tool holder equipped with a thermocouple at a sampling time of 1/30 of a second. We confirmed that the temperature measured by the wireless tool holder agrees with that measured by infrared thermographic imagery. As a result, we demonstrated that the developed method with a wireless system is effective to estimate the tool temperature in end-milling processes and makes it feasible to measure it under coolant conditions.

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Puparia Cleaning Techniques for Forensic and Archaeo-Funerary Studies

Insects ◽

10.3390/insects12020104 ◽

2021 ◽

Vol 12 (2) ◽

pp. 104

Author(s):

Jennifer Pradelli ◽

Fabiola Tuccia ◽

Giorgia Giordani ◽

Stefano Vanin

Keyword(s):

Sodium Hydroxide ◽

Molecular Identification ◽

Sodium Hydroxide Solution ◽

Species Level ◽

Post Mortem ◽

Molecular Analyses ◽

Chemical Treatments ◽

Wrong Identification ◽

Physical And Chemical

Diptera puparia may represent both in forensic and archaeo-funerary contexts the majority of the entomological evidence useful to reconstruct the peri and post-mortem events. Puparia identification is quite difficult due to the lack of identification keys and descriptions. In addition, external substances accumulated during the puparia permanence in the environment make the visualization of the few diagnostic characters difficult, resulting in a wrong identification. Six different techniques based on physical and chemical treatments have been tested for the removal of external substances from puparia to make identification at species level feasible. Furthermore, the effects of these methods on successful molecular analyses have also been tested as molecular identification is becoming an important tool to complement morphological identifications. The results of this study indicate that cleaning via warm water/soap, the sonication and treatment with a sodium hydroxide solution are the best methods to achieve a good quality of the samples.

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