scholarly journals Correlation between Antibacterial Activity and Free-Radical Scavenging: In-Vitro Evaluation of Polar/Non-Polar Extracts from 25 Plants

Processes ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 117 ◽  
Author(s):  
Mahmoud Rayan ◽  
Baheer Abu-Farich ◽  
Walid Basha ◽  
Anwar Rayan ◽  
Saleh Abu-Lafi
Keyword(s):  
Antibacterial Activity ◽  
Free Radical ◽  
Plant Species ◽  
Plant Extracts ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Wild Plant ◽  
Foeniculum Vulgare ◽  
Highly Active ◽  
Depth Analysis

Objectives: The current study aimed to measure the antioxidant and antibacterial activities of 25 wild Palestinian edible plants, which were subjected to extraction by polar and non-polar solvents. Correlations between free radical scavenging activity and antibacterial activity of the extracts were assessed for both polar and non-polar fractions. Materials: Twenty-five wild edible plant species that are frequently consumed by people in Palestine (mainly in a rural area) were examined. Among them, 10 plant species were among those with the highest mean cultural importance values, according to an ethnobotanical survey that was conducted in the West Bank, Palestine, a few years ago. Method: The protocol of the DPPH assay for testing free-radical scavenging was utilized for determining EC50 values, while microdilution tests were conducted to determine the 50% inhibitory concentration (IC50) of the extracts for the microorganism Staphylococcus mutans. Results and Discussion: Eight extracts (non-polar fractions) were found to possess an antibacterial IC50 of less than 20 ppm, such as Foeniculum vulgare, Salvia palaestinafruticose, Micromeria fruticose, Trigonella foenum-graecum, Cichorium pumilum jacq, Salvia hierosolymitana boiss, Ruta chalepensis, and Chrysanthemum coronarium. The polar fractions possess higher antioxidant activity, while non-polar fraction possess higher antibacterial activity. Looking at all the results together can deceive and lead to the conclusion that there is no correlation between antibacterial activity against S. mutans and free radical scavenging (R2 equals 0.0538). However, in-depth analysis revealed that non-polar plant extracts with an EC50 of free radical scavenging ≤100 ppm have a four-fold order of enrichment toward more activity against S. mutans. These findings are of high importance for screening projects. A four-fold order of enrichment could save plenty of time and many in screening projects. The antibacterial active extracts marked by low-medium free radical scavenging might act through a mechanism of action other than that of highly active, free radical scavenging extracts. Conclusion: The screening of antioxidant and antimicrobial activity performed on 25 selected wild plant extracts revealed a satisfactory free radical scavenging and antimicrobial potential that could be of value in the management of oxidative stress. Further studies are recommended to explore novel and highly active natural antibacterial products.

scholarly journals Bioactivity of extracts of Centaurea polyclada dc. (Asteraceae)

2009 ◽  
Vol 61 (3) ◽  
pp. 447-452 ◽  
Author(s):  
Melanie Granger ◽  
Emilie Samson ◽  
Severine Sauvage ◽  
Anisha Majumdar ◽  
Poonam Nigam ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Free Radical ◽  
Solid Phase Extraction ◽  
Brine Shrimp ◽  
Solid Phase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phase Extraction ◽  
Brine Shrimp Lethality Assay

The free radical-scavenging property, antibacterial activity, and brine shrimp toxicity of n-hexane, dichloromethane (DCM), and methanol (MeOH) extracts of Centaurea polyclada, an endemic Turkish species, were assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, the resazurin microtiter plate-based assay, and the brine shrimp lethality assay, respectively. The DCM and MeOH extracts of C. polyclada exhibited free radical-scavenging ability with RC50 values 1.17 and 0.015 mg/mL, respectively. Among solid-phase extraction fractions of the MeOH extract, the fraction eluted with 60% MeOH in water demonstrated the highest level of free radical-scavenging activity (RC50 = 0.016 mg/mL). Only the DCM extract showed considerable antibacterial activity against all nine test strains except Escherichia coli, with MIC ranging from 1.25 to 2.50 mg/mL. This antibacterial activity pattern was also observed with solid-phase extraction fractions of the DCM extract with varied potencies. None of the extracts showed any significant toxicity towards brine shrimps (LD50 = >1.00 mg/mL).

scholarly journals Free radical scavenging activity of Mozambican plant extracts

2015 ◽  
Vol 98 ◽  
pp. 185 ◽  
Author(s):  
I.T. Madamombe-Manduna ◽  
N.T. Mthakathi ◽  
S.S. Mashele ◽  
R. Chitondo ◽  
R.J. Matavele-Chissumba
Keyword(s):  
Free Radical ◽  
Plant Extracts ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity

Evaluation of Antioxidant Activities of Alpinia galanga (L.) Willd

2018 ◽  
Vol 15 (4) ◽  
pp. 899-908
Author(s):  
Khoirom Ratipiyari Devi ◽  
Paonam Priyobrata Singh ◽  
Moirangthem Medhapati Devi ◽  
Gurumayum Jitendra Sharma
Keyword(s):  
Free Radical ◽  
Plant Extracts ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Alpinia Galanga ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Promising Source

Present research was designed to evaluate the free radical scavenging capacities and antioxidant activities of rhizome extracts of Alpinia galanga prepared in different solvent systems (60% aqueous methanol, 60% aqueous ethanol and distilled water) using different in vitro chemical assays. Antioxidant components such as total phenolic content (TPC), total flavonoid content (TFC) and ascorbic acid contents of the ginger species were screened. Antioxidant assays employed included sulphur free radical reactivity assay, ferric ion reducing power assay, DPPH free radical scavenging capacity assay, hydroxyl radical scavenging assay, nitric oxide scavenging activity assay and hydrogen peroxide scavenging assay. The obtained data reveal that the plant extracts contained significant amount of the observed antioxidant components and also exhibited significant free radical scavenging capacities. Methanol (60%) extract exhibited highest antioxidant activity than other solvents. The polyphenolic constituents of the plant extracts appear to be largely responsible for the radical scavenging capacity. The plant extracts act as promising source of antioxidants, and may be useful for development of nutraceuticals and pharmaceutical drugs.

scholarly journals Bioactivity of Centaurea persica boiss. (Asteraceae)

2012 ◽  
Vol 64 (2) ◽  
pp. 517-523 ◽  
Author(s):  
Satyajit Sarker ◽  
Lutfun Nahar ◽  
Srikanth Gujja ◽  
Shazi Begum ◽  
Sezgin Celik
Keyword(s):  
Antibacterial Activity ◽  
Free Radical ◽  
Solid Phase ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Disc Diffusion ◽  
Dpph Assay ◽  
Meoh Extract ◽  
E Coli ◽  
Diffusion Assay

The free-radical-scavenging property, antibacterial activity and brine shrimp toxicity of petroleum ether (PE), dichloromethane (DCM) and methanol (MeOH) extracts of Centaurea persica, a Turkish medicinal plant, were assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, the resazurin microtiter plate based assay, and the brine shrimp lethality assay, respectively. Additionally, the disc diffusion assay was also used to assess antibacterial activity. Only the MeOH extract of C. persica exhibited a significant free-radical-scavenging property in the DPPH assay, with an RC50 value of 0.025 mg/mL. However, in the TLC-based qualitative assay, petroleum ether and DCM extracts showed an extremely low level of free-radical-scavenging property. Among the solid-phase extraction fractions of the MeOH extract, the fractions eluted with 60% and 80% MeOH in water exhibited the highest level of free-radicalscavenging activity (RC50 = 0.010 and 0.015 mg/mL, respectively). While DCM extract showed reasonable antibacterial activity against five out of the nine test strains both in the disc diffusion assay and in the resazurin assay, the MeOH extract was highly active against both Escherichia coli and ampicillin-resistant E. coli strains. Among the solid-phase extraction fractions of the MeOH extract, fractions eluted with 80% MeOH in water and 100% MeOH displayed significant antibacterial potencies against both E. coli species. None of the extracts showed any significant toxicity towards brine shrimps (LD50 = >1.00 mg/mL).

scholarly journals EVALUATION OF ANTIOXIDANT, ANTIMICROBIAL, AND ANTIFUNGAL POTENTIAL OF CUCURBITA PEPO VAR. FASTIGATA SEED EXTRACTS

2019 ◽  
pp. 289-293
Author(s):  
ROSHNI RS SONI ◽  
MANOJ BALI
Keyword(s):  
Antibacterial Activity ◽  
Free Radical ◽  
Cucurbita Pepo ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Seed Extract ◽  
Scavenging Activity ◽  
Antifungal Potential

Objective: The current study aims to study the antioxidant and antimicrobial and antifungal potential of the methanolic extract of Cucurbita pepo var. fastigata seeds (MECS). Methods: Extraction of the seeds has been carried out with solvents of increasing polarity (chloroform, acetone, and methanol) and the phytochemical study of the methanolic extract have been carried out using standard methods. The free radical scavenging activity of all the extracts was evaluated by DPPH and H2O2 methods. Standard disk diffusion method was used to evaluate antibacterial and antifungal activities. Results: Phytochemical evaluation showed the maximum presence of triterpenoids, phenolic compounds, tannins and small amount of Coumarins. Methanolic extract revealed momentous antioxidant activity as compared to chloroform and ethyl acetate extract. Hence, methanolic extract of C. pepo. seeds (MECS) at a dose level of 100, 200 and 300 μg/ml was evaluated for antioxidant potential. Maximum free radical scavenging activity of methanolic seed extract of cucurbita pepo var. fastigata has been found at a dose of 300 μg/ml to be 63±0.16 % by 1,1-diphenyl-2-picryl hydrazyl model and at a value of 78% at 300 μg/ml with H2O2 model. Methanolic extract also showed the presence of antibacterial activity. Conclusion: Presence of phytochemicals in the methanolic extract is responsible for the antioxidant potential. Extracts were investigated for antibacterial activity using the standard disc diffusion assay method against Bacillus subtilis, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa and for the antifungal activities against Aspergillus niger and Candida albicans. The seed extract showed the presence of antibacterial activity, but the antifungal activity was found to be absent in the extract.

scholarly journals Free Radical Scavenging and Antibacterial Activities, and GC/MS Analysis of Essential oils from Different Parts of Falcaria vulgaris from Two Regions

2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Ali Shafaghat
Keyword(s):  
Antibacterial Activity ◽  
Free Radical ◽  
Essential Oils ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Free Radical Scavenging ◽  
Major Constituent ◽  
Falcaria Vulgaris ◽  
Leaf Oil ◽  
Different Parts

The chemical composition of the essential oils obtained by hydrodistillation from flower, leaf and stem of Falcaria vulgaris Bernh., from two different localities in Iran, were analyzed by GC and GC/MS (sample A from Ardabil and sample B from Khalkhal area). α-Pinene was the major constituent of the flower, leaf and stem oils from sample A: (43.8%, 33.0% and 50.9%, respectively). Of the 10 components detected in the oil of F. vulgaris flower, high amounts of β-caryophyllene (25.2%) and 1,8-cineole (12.8%) were recorded. α-Terpinyl acetate (23.2%) and limonene (14.4%) were major constituents of the leaf oil. In the oils of sample B: α-pinene (16.1% in the flower oil, 31.5% in the leaf oil and 34.5% in the stem oil) was again the major compound. Limonene (14.2%) and germacrene D (32.1%) were also main constituents of the leaf oil. α-Terpinyl acetate (21.9% in the leaf oil) and limonene (29.8% in stem oil) were the other major compounds obtained from this sample. Antibacterial activity was determined by measurement of growth inhibitory zones, and the antioxidant activity was tested using the DPPH radical scavenging method. The essential oils from different parts and localities of Falcaria vulgaris showed free radical scavenging and antibacterial activity.

scholarly journals In vitro screening of antioxidant, free radical scavenging and antimicrobial potential of Micrococca mercurialis whole plant extracts

2019 ◽  
Vol 10 (4) ◽  
pp. 3251-3262
Author(s):  
Thendral Hepsibha B ◽  
Gayathri Devi R ◽  
Lijitha L
Keyword(s):  
Free Radical ◽  
Plant Extracts ◽  
Skin Diseases ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Whole Plant ◽  
Anti Inflammatory ◽  
Scientific Validation

Micrococca mercurialis is widely used for the treatment of sores, skin diseases, cold, fever, rheumatic pain, and infections. The present study aimed to study the antioxidant, free radical scavenging and antibacterial potential different whole plant extracts (aqueous, ethanol, petroleum ether) of Micrococca mercurialis by in vitro methods. The amount of phenols, tannins, flavonoids and Vitamin C were estimated by conventional methods. Free radical scavenging potential was assessed by DPPH and FRAP assays. HRBC membrane-stabilizing study was done to determine its anti-inflammatory property by in vitro method. Activity against Staphylococcus aureus and Bacillus subtilis were determined in Micrococca mercurialis extracts. The phytoconstituents in the extracts of Micrococca mercurialis were explored by GC-MS analysis. The extracts of Micrococca mercurialis exhibited varied in vitro antioxidant, free radical scavenging, antibacterial and anti-inflammatory activities. The GC–MS results confirmed the presence of twenty-nine phytoconstituents totally responsible for the biological activities of Micrococca mercurialis. This pilot study has provided a scientific validation for the folkloric use of Micrococca mercurialis against many infections and diseases.

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